Abstract: The invention relates to new asparaginases having improved properties, preferably improved thermotolerance, such as improved activity at high temperatures and/or improved thermostability. The invention also relates to DNA sequences encoding such improved asparaginases, their production in a recombinant host cell, as well as methods of using the asparaginases, in particular for reduction of acrylamide in foods. The invention furthermore relates to methods of generating and preparing asparaginase variants having improved properties.
Type:
Grant
Filed:
November 6, 2009
Date of Patent:
November 9, 2010
Assignee:
Novozymes A/S
Inventors:
Tomoko Matsui, Esben Peter Friis, Akihiko Yamagishi
Abstract: The present invention relates to subtilase subtilases with an altered immunogenicity, particularly subtilases with a reduced allergenicity. Furthermore, the invention relates to expression of said subtilase variants and subtilases and to their use, such as in detergents and oral care products.
Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.
Type:
Grant
Filed:
September 21, 2005
Date of Patent:
October 19, 2010
Assignee:
Novozymes A/S
Inventors:
Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.
Type:
Grant
Filed:
September 21, 2005
Date of Patent:
October 12, 2010
Assignee:
Novozymes A/S
Inventors:
Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
Abstract: The present invention relates to a new wild-type lipase, its nucleic acid sequences and the amino acid sequences thereof. This new lipase shows washing performance in the form of wild-type. The present invention also relates to the method for producing the lipase and the uses of the lipase in baking.
Type:
Grant
Filed:
July 6, 2007
Date of Patent:
August 24, 2010
Assignee:
Novozymes A/S
Inventors:
Xiao Mei Niu, Ye Liu, Jurgen Carsten Franz Knotzel, Signe Munk
Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.
Type:
Grant
Filed:
September 21, 2005
Date of Patent:
June 22, 2010
Assignee:
Novozymes A/S
Inventors:
Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
Abstract: A method for testing cleaning effect of a compound or compositions is disclosed. The method comprises: (a) Preparing a liquid sample of less than 10 ml comprising the test compound, (b) applying liquid sample to a stained surface, (c) applying mechanical stress to the stained surface by contacting it with a body present in the liquid sample, (d) evaluating the cleaning effect of applying solution and mechanical stress on the stained surface.
Type:
Grant
Filed:
June 26, 2006
Date of Patent:
June 15, 2010
Assignee:
Novozymes A/S
Inventors:
Georg Rudolf Theobald Bechmann, Steven Paul Georges Cooremans, Michael Godskesen, Soren Kjaerulff
Abstract: The invention relates to new asparaginases having improved properties, preferably improved thermotolerance, such as improved activity at high temperatures and/or improved thermostability. The invention also relates to DNA sequences encoding such improved asparaginases, their production in a recombinant host cell, as well as methods of using the asparaginases, in particular for reduction of acrylamide in foods. The invention furthermore relates to methods of generating and preparing asparaginase variants having improved properties.
Type:
Grant
Filed:
March 7, 2008
Date of Patent:
February 23, 2010
Assignee:
Novozymes A/S
Inventors:
Tomoko Matsui, Esben Peter Friis, Akihiko Yamagishi
Abstract: The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.
Type:
Grant
Filed:
July 9, 2008
Date of Patent:
December 29, 2009
Assignee:
Novozymes A/S
Inventors:
Kim Borch, Luise Erlandsen, Jesper Vind, Allan Svendsen, Christel Thea Jorgensen
Abstract: The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
Type:
Grant
Filed:
December 6, 2007
Date of Patent:
December 15, 2009
Assignee:
Novozymes A/S
Inventors:
Kirsten Bojsen, Allan Svendsen, Claus Crone Fuglsang, Shamkant Anant Patkar, Kim Borch, Jesper Vind, Andreas Petri, Sanne O. Schroder Glad, Gitte Budolfsen
Abstract: The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.
Type:
Grant
Filed:
April 29, 2004
Date of Patent:
December 16, 2008
Assignee:
Novozymes A/S
Inventors:
Kim Borch, Luise Erlandsen, Jesper Vind, Allan Svendsen, Christel Thea Jorgensen