Abstract: Specified classes and subclasses of leukocyte blood cells are identified by immunohematology procedures, based on utilization of antigenic determinants on the cell surface, their reactivity with antibodies which fluoresce under known circumstances, and identified by utilization of principles of flow cytometry or morphology.This invention particularly concerns improvements in the lysing and fixing method used prior to detection and identifying of the cells. In this method, a whole blood sample first is incubated with a reagent including antibodies to the cell subclass to be identified, the antibodies having directly or indirectly made fluorescently responsive to a particular light (e.g. argon ion laser). The red blood cells then are lysed with a reagent containing saponin. Next follows a leukocyte fixing treatment, preferably using a cross-linking dialdehyde composition, such as glyoxal or glutaraldehyde.

Abstract: An isotonic multipurpose blood diluent, and a method for use of this diluent with a weak lysing reagent system which is especially suitable for routine enumeration of traditional hemogram values, and also the determination of lymphoid-myeloid populations of leukocytes, particularly in automatic particle counting systems.This blood diluent is capable of affording accurate, reproducible test results. It is an osmotically balanced aqueous solution of preselected pH containing Procaine hydrochloride for maintaining erythrocyte morphology during operation, N-(2-acetamido)iminodiacetic acid (ADA) as a blood cell stabilizing agent, and bacteriostatic agents including sodium 1-hydroxypyridine-2-thione, and dimethylolurea which, together with the ADA, allow preferential determination of myeloid-lymphoid leukocytes, and other hematological values.The lysing agent is a mixture of an aqueous solution of at least one quaternary ammonium salt having surface active properties, and an alkali metal cyanide.

Abstract: Liquid acid anhydrides are excellent curatives for epoxy resins but state of the art processing methods require cures at temperatures in excess of 100.degree. C. to obtain well cured polymers. It has been found that a resinous liquid polyol prepared from a polyol and an epoxy resin in the presence of a quaternary ammonium compound acts as an excellent promoter for the epoxy resin anhydride reaction producing well cured polymers at temperatures of about 70.degree. C. The use of a metallo-organic compound in addition to the resinous polyol provides for rapid gelation and development of cure at 70.degree. C.

Abstract: This invention describes a reagent system, including saponin in a lysing reagent and a rapidly active cross-linking agent such as glutaraldehyde as a fixing reagent, which reproducably affects whole blood to cause the red blood cells to stromatolyze and modifies the leukocytes to generate data to define four distinct clusters for detection and classification by flow analysis instrumentation. The clusters represent the four major leukocyte types found in blood: lymphocytes, monocytes, neutrophils and eosinophils, thus providing a method of leukocyte differential analysis. The parameters used for the leukocyte classification include combinations of two or more of DC (Coulter) volume, high frequency (RF) size, opacity (RF size/DC volume), light scatter at various angular ranges, and fluorescence at various wavelengths of illumination.

Abstract: This invention primarily is directed to a hematology reference control solution, the three separate white cell control portions thereof consisting of three types of fixed red cells of determined size distribution for checking the operation of a particle analyzing instrument, including its predetermined lower and upper threshold settings for each class or subclass of leukocytes.For preparing a human granulocyte analogue, nurse shark erythrocytes are altered and fixed in a chilled solution to simulate in number, size and distribution the granulocytes in human whole blood.

Abstract: A method is described for stabilizing the viscosity of a liquid epoxy-anhydride system which is a blend of a liquid epoxy resin, a liquid anhydride curing agent and a quaternary ammonium salt promoter for an epoxy-anhydride reaction. This system is especially useful in the manufacture of tubing, piping and other geometric structures. The method employs at least one of the following modifications:(1) adding a minor amount of a solid epoxy resin to the liquid epoxy resin;(2) adding to the liquid anhydride a minor amount of solid anhydride of high reactivity;(3) adding to the epoxy-anhydride system a solid resinous particulate material in finely ground form which is capable of reacting with the epoxy resin and acid anhydride, and is soluble in the epoxy-anhydride system at temperatures below the gelation temperature; and(4) adding a metallo-organic compound which lowers the initiation temperature or accelerates the epoxy-anhydride reaction.

Abstract: A method and reagent system is described for differential determination of more that one class of leukocyte populations, using an automatic blood cell analyzer. The reagent system includes a blood diluent and stromatolyzing reagent. The blood diluent is an osmotically balanced aqueous solution of ingredients for maintaining erythrocyte morphology, blood cell stabilizing, buffering and bacteriostatic action. The stromatolyzing reagent is a mixture of an aqueous solution of quaternary ammonium salts, a chromagen forming agent and an additive which is a non-cationic surfactant. The quaternary ammonium salts, with the additive, serve the purpose of positioning, relative to one another and a volume reference point, the lymphoid and myeloid populations. The additive also acts to prevent or to reduce the amount of protein deposit in the sensing orifices of the analyzer, which deposit tends to accumulate from the cell debris resulting from blood cell stromatolyzation.

Abstract: An isotonic multipurpose blood diluent, and a method for use of this diluent with a weak lysing reagent system which is especially suitable for routine enumeration of traditional hemogram values, and also the determination of lymphoid-myeloid populations of leukocytes, particularly in automatic particle counting systems.This blood diluent is capable of affording accurate, reproducible test results. It is an osmotically balanced aqueous solution of preselected pH containing Procaine hydrochloride for maintaining erythrocyte morphology during operation, N-(2-acetamido)iminodiacetic acid (ADA) as a blood cell stabilizing agent, and bacteriostatic agents including sodium 1-hydroxypyridine-2-thione, and dimethylolurea which, together with the ADA, allow preferential determination of myeloid-lymphoid leukocytes, and other hematological values.The lysing agent is a mixture of an aqueous solution of at least one quaternary ammonium salt having surface active properties, and an alkali metal cyanide.

Abstract: A method and reagent system is described for differential determination of three-populations of leukocytes (lymphocyte, monocyte and granulocyte), using an automatic counting system. The reagent system includes a blood diluent and lysing reagent. The blood diluent is an osmotically balanced aqueous solution of ingredients at a preselected pH for maintaining erythrocyte morphology, blood cell stabilizing, buffering and bacteriostatic action. The lysing reagent is a mixture of an aqueous solution of quaternary ammonium salts, which lysing reagent is added to the diluted blood under more mild conditions of concentration and at a slower rate than is current practice in order to obtain an unexpected volumetric modification of at least one of the three-populations of leukocytes, whereby volumetric differential analysis can be accomplished.

Abstract: This invention utilizes for stabilization of platelets a combination of iodoacetamide and an iminodiacetic acid or salt thereof, together with a compatible bacteriostatic agent, in an aqueous electrolytic solution which is maintained at a preselected range of pH and osmolality.In a preferred formulation, the stabilizing solution contains iodoacetamide, N-(2-acetamido)iminodiacetic acid and sodium penicillin. Ethylenediaminetetraacetic acid, or salts thereof, are optionally present as an additional ingredient.

Abstract: This invention relates to a device for use in straightening or relaxing overly-curly or wavy, unmanageable hair, using caustic compositions which can irritate the scalp or skin if brought into contact therewith. The device includes a planar base member or board, adjustable for head size and hair length, with means for covering with paper or similar material which overlaps the top edge of the board. The board covered with the paper is placed next to the scalp and the hair overlaps the board and paper at the top edge. The paper is cut off at a distance from the length of a lock of hair placed thereupon. The relaxing composition is then applied to the lock of hair and worked into the hair for suitable time, as determined by the operator. The board is then removed and the process repeated for another lock of hair.

Abstract: An isotonic cleansing agent and method for its use in electronic instrumentation systems for determining hematological components of blood samples are described. The cleansing agent comprises an aqueous solution of a non-hemolytic polyoxyethylated alkylphenol detergent, dimethylolurea, and an isotonic and isoconductive saline base which is free of phosphates. The dimethylolurea serves as a cell stabilizer, and also acts as a germicidal agent, together with other added germicidal agents, particularly sodium 1-hydroxypyridine-2-thione.

Abstract: An isotonic multipurpose blood diluent, and a method for use of this diluent with a weak lysing reagent system which is especially suitable for routine enumeration of traditional hemogram values, and also the determination of lymphoid-myeloid populations of leukocytes, particularly in automatic particle counting systems.This blood diluent is capable of affording accurate, reproducible test results. It is an osmotically balanced aqueous solution of preselected pH containing Procaine hydrochloride for maintaining erythrocyte morphology during operation, N-(2-acetamido)iminodiacetic acid (ADA) as a blood cell stabilizing agent, and bacteriostatic agents including sodium 1-hydroxypyridine-2-thione, dimethylolurea and chlorhexidene diacetate which, together with the ADA, allow preferential determination of myeloid-lymphoid leukocytes, and other hematological values.

Abstract: A decorative wall panel for use individually as an accent piece, or for mounting in multiples to a wall or the like, to define a decorative wall area such as, for example, around an open fireplace. Each panel is formed of a suitable sheet metal material, such as copper, including a main panel area, bounded by peripheral turned-in edge portions to nest a support block. The main panel area is randomly subdivided into smaller, variously sized and configurated panel portions by grooves formed therein which are filled with a suitable material such as solder; and various individual panel portions are provided with design characterists in relief while various colorations may be selectively applied to any of the panel portions.

Abstract: A card game for amusement requiring an active physical participation of the players causing exercising of the human body, comprising a pair of deck of cards of a substantial rectangular shape, said cards of one of said pair of deck of cards having the word Toucher printed on a back side thereof and the other of said pair of deck of cards having the word Touchee printed on a back side thereof for being stacked into two different and distinct stacks of cards, said cards of said pair of deck of cards each having a pictorial representation of a human body part on a front playing side thereof, whereby one player who picked a card from the toucher deck of cards touches with his body part according to the human body part shown on the front playing side of said card the body part of another player according to the human body part shown on the front playing side of a card picked by the other player from the touchee deck of cards thereby causing the players to assume various physical positions resulting in a physical e

Abstract: A process for preparing whole blood reference controls having long term stability up to six months for devices using electronic means for whole blood determinations including platelet count and mean cell volume and red blood distribution width. The preconditioning diluent for the red blood cells consists essentially of an aqueous solution of lactose, sodium azide, and a non-ionic surfactant and is pH buffered and osmolality adjusted. The media of the whole blood control includes lactose, fungicides and antibiotics, and supplementary agents including purine nucleosides. It also includes additional components which alter red blood cell membranes including bile salts and cholic acid derivatives, phenothiazine compounds and the salts thereof having antihistamine properties, and 4-aminobenzoic acid ester derivatives and their salts having local anesthetic properties.

Abstract: This invention relates to a lytic diluent and a method for rapid lysing of red blood cells in whole blood for a differential determination of lymphoid and myeloid populations of leukocytes, and measurement of hemoglobin, particularly for use in automatic particle counting systems.The diluent is a mixture of at least one quaternary ammonium salt having surface active properties, and an additive in suitable proportions, buffered to an acid pH of 3.5 to 5.0. The additive includes 2-phenoxyethanol and other short chain alkanols substituted by one phenyl or phenoxy, as well as certain polyhydroxy compounds.When the reagent is employed in lower concentrations, an alkaline reagent comprising a quaternary ammonium salt and an alkali metal cyanide, buffered to an alkaline pH, may be added to complete the chromagen formation.

Abstract: A method is described for preparing a human platelet analog for use as a reference control in particle analysis instrumentation of the Coulter type. Goat erythrocytes from individual goats which have been selected with respect to age, sex, selective breeding, a controlled environment, prior veterinary treatment, and diet are altered in size and/or combined with samples from several goats, as needed, to obtain a blend for use as a reference control for automated particle counting equipment in which the goat erythrocytes simulate in number, size and distribution the platelets in human whole blood. The controls are useful as a free-standing reference for determination of human platelets only, or can be added to whole blood reference controls for multi-parameter instruments.

Abstract: An in situ turbidity meter, for measuring water quality of a body of water comprising a pulsed infrared laser which illuminates the water, thereby producing scattered radiation from particles suspended in the water. A portion of the scattered radiation is collected and correlated to the turbidity of the water.

Abstract: A stable calibrator composition, useful in equipment for determining hematological values in blood samples, comprises a hemolysate of red blood cells which have been lysed with a dilute aqueous solution of saponin to which has been added a halophenol that has a phenol coefficient of between 20 and 90 to provide a concentration from about 1:1000 to about 1:1750 w/v of said halophenol in said hemolysate, said calibrator composition having a pH between 4 and 7. The preferred halophenol is 4-chloro-3,5-xylenol.