Abstract: The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.

Abstract: The invention is directed to a security element for the electronic surveillance of articles. This security element is detected by surveillance systems operating in different frequency ranges and it includes at least two materials of high permeability which, when excited by an external alternating magnetic field emit each a characteristic signal. The maximum signal components of the two materials lie in different frequency ranges and the signal components of the one material are negligibly ow in that particular frequency range in which the signal component of the other material is at a maximum level. The invention also relates to surveillance systems that incorporate these security elements.

Abstract: In order to improve precision and reliability of a hydraulic pressure control apparatus for a brake system of a vehicle, this invention provides a hydraulic pressure control apparatus for a brake system of a vehicle including a control unit for controlling operation of the hydraulic pressure control apparatus, a pressurized fluid generating unit for pressurizing brake fluid and supplying the pressurized fluid, and a hydraulic pressure distributing unit for mechanically selecting and transmitting at least one of hydraulic pressure of the pressurized fluid supplied from the pressurized fluid generating unit and hydraulic pressure supplied from a master cylinder of the brake system to a wheel brake cylinder of the brake system.

Abstract: A novel predator bacterium Burkholderia casidae is disclosed. The invention is directed to the isolation and use of Burkholderia casidae to control microbial diseases of plants. The genetic, biochemical and physiological characteristics of Burkholderia casidae are described. Biocontrol compositions comprising Burkholderia casidae, and antimicrobial compounds and antimicrobial preparations prepared from Burkholderia casidae are also disclosed, as are methods for accomplishing all of the foregoing.

Abstract: There is a time delay between the moment of fuel injection and the moment when air with the injected fuel is drawn into cylinders. At the time of fuel injection, air-mass that will be drawn into cylinders after the delay time is estimated on the basis of expectation values of throttle opening, air-mass drawn into an intake-manifold, and intake-manifold pressure. Fuel amount is in turn calculated based on the estimated air-mass.

Abstract: The present invention is directed to the isolation and use of super-infective, tumor-specific vectors that are strains of parasites including, but not limited to bacteria, fungi and protists. In certain embodiments the parasites include, but are not limited to, the bacterium Salmonella spp., such as Salmonella typhimurium, the bacterium Mycobacterium avium and the protozoan Leishmania amazonensis. In other embodiments, the present invention is concerned with the isolation of super-infective, tumor-specific, suicide gene-containing strains of parasites for use in treatment of solid tumors.

Abstract: A position tracking system for a plurality of mobile tractors and trailers includes an unique electronic identifier for each of a plurality of trailers. For each tractor of a plurality of tractors, the position tracking system includes a navigation system for determining a current position of the tractor, an electronic identifier reader for acquiring the electronic identifier of a trailer coupled to the tractor, and a position-reporting subsystem for reporting to a remote system the current position of the tractor and the electronic identifier of the trailer coupled to the tractor. In addition, the position tracking system includes, at each facility of a plurality of fixed-location facilities, one or more electronic identifier readers for acquiring the electronic identifier of one or more trailers located within the facility, and a trailer-presence reporting subsystem for reporting to the remote system the presence of the one or more trailers located within the facility.

Abstract: An engine ladder frame with minimized noise and vibrations, improved stability of the oil filter, and reduced air in the oil flowing from the cylinder head to the oil pan. Inside an elongate rectangular outer block, three partitioning walls are formed equidistantly, so as to form four crank chambers. The crank chambers respectively have bottom faces which are not perforated or open but are completely closed. On the side parts of the bottom faces, there are respectively formed oil flow faces which gradually become deeper in the direction of the revolutions of the crankshaft. Among the oil flow faces, those of the first, second and third crank chambers have oil drain holes respectively at their ends. Thus the bottoms of the crank chambers are closed so as to close the space between the cylinder block and the oil pan, resulting in the reduction of the noise and vibrations.

Abstract: A programmable I/O element for an I/O terminal of a logic array is suitable for operating according to high speed I/O modes such as double data rate and zero bus turnaround. The I/O element may include an input block with two registers for registering input signals from the terminal upon alternate clock edges. In addition or alternatively, it may include an output block with two registers that separately register output signals from the array on the same clock edge and a multiplexer that alternately outputs those signals. For bidirectional terminals, the multiplexer output is connectable to the I/O terminal via an output buffer, and an output enable block provides an enable signal to a gating input of the output buffer. Programmable delays may be included in the input, output, and output enable paths, in particular to provide a slower turn-on time than turn-off time for the output buffer.

Abstract: A clock signal desired to be transmitted to various components of the electronic system is combined with a noise signal to generate a spread spectrum clock signal which, in turn, is distributed with an associated reference signal to selected components of the system using two-channel communication links. A receiving circuit within each of the selected components recovers the original clock signal from the spread spectrum clock signal and its associated reference signal. In one embodiment, the clock signal is combined with the noise signal in an exclusive-OR logic gate to generate a spread spectrum clock signal which is distributed to receiving components using a first channel. The noise signal is transmitted as the reference signal using the second channel. The two channel signals are combined in an exclusive-OR gate of one or more receiving circuits to recover the clock signal.

Abstract: The present invention relates to a purified, easily produced poly-&bgr;-1→4-N-acetylglucosamine (p-GlcNAc) polysaccharide species. The p-GlcNAc of the invention is a polymer of high molecular weight whose constituent monosaccharide sugars are attached in a &bgr;-1→4 conformation, and which is free of proteins, and substantially free of single amino acids, and other organic and inorganic contaminants. In addition, derivatives and reformulations of p-GlcNAc are described. The present invention further relates to methods for the purification of the p-GlcNAc of the invention from microalgae, preferably diatom, starting sources. Still further, the invention relates to methods for the derivatization and reformulation of the p-GlcNAc. Additionally, the present invention relates to the uses of pure p-GlcNAc, its derivatives, and/or its reformulations.

Abstract: The change in the circular birefringence of a sample is measured by passing a light beam comprised of a left circularly polarized (LCP) wave and a right circularly polarized (RCP) wave through a sample and measuring the change in the phase difference between the RCP and LCP waves.

Abstract: Compounds that modulate the estrogen receptor (ER) are disclosed, as well as pharmaceutical compositions containing the same. In a specific embodiment, the compounds are selective modulators for ER-&bgr; over ER-&agr;. Methods are disclosed for modulating ER-&bgr; in cell and/or tissues expressing the same, including cells and/or tissue that preferentially express ER-&bgr;. More generally, methods for treating estrogen-related conditions are also disclosed, including conditions such as is breast cancer, testicular cancer, osteoporosis, endometriosis, cardiovascular disease, hypercholesterolemia, prostatic hypertrophy, prostatic carcinomas, obesity, hot flashes, skin effects, mood swings, memory loss, urinary incontinence, hairloss, cataracts, natural hormonal imbalances, and adverse reproductive effects associated with exposure to environmental chemicals.

Abstract: A shape memory alloy step drive mechanism includes at least one shape memory alloy element (1) with a fixed end, the other end being connected to one end of a lever (2). The lever (2) is provided with a pawl (4) which is located to move on the teeth of a ratchet wheel (5) mounted on a main shaft (6) along with the lever (2). A stopper pin (7) is provided which also guides the pawl (4) on the ratchet wheel (5). A bias spring (9) is provided on a mandril on the main shaft (6) for returning the lever (2) to its original position. A detent wheel (8) with at least one detent lever (10) is mounted on the main shaft (6) to provide the detent torque required to hold the main shaft (6) in position when the lever (2) returns to its original position. A pair of support brackets (11) are provided for supporting and holding the various components together in an assembly.

Abstract: A postage printing system enables users to generate postage indicia and print mail pieces at client computers. Upon user determination of a mail piece print failure, a mail metering application on the client computer sends reprint request to an authorizing authority at a remote location. The reprint request includes information identifying an account for printing a postage indicia. A server computer used by the authorizing authority receives the reprint request from a client computer and evaluates the request. When the account is verified and other criteria are satisfied, a reply is sent to the client computer that includes a reprint authorization. Otherwise, a negative reply is sent to the client computer that does not include the reprint authorization. Upon receiving a reprint authorization from the authorizing authority, the client computer prints a replacement mail piece with the postage indicia.

Abstract: A method for performing a transaction without the use of spoken communication is disclosed. The method consists of providing at least one transactional flash card having information about the transaction and the transaction goal displayed on the card in text understandable to the user and different text understandable to the party from whom the user wishes to purchase goods or services. The method may include displaying such a flash card in printed form, or on a personal electronic device. The method may further include providing flash cards having inquiries about price, payment methods, or shipping terms. Also disclosed are transactional flash cards used with the method.

Abstract: When a reflective wave reflected from a preceding object is received, the reflective wave being of an original radar beam scanned at a variable vertical angle, the vertical angle is detected, an absolute speed of the preceding object is calculated based on a vehicle speed and a relative speed calculated from the reflective wave, and accordingly a driving environment is determined based on the vertical angle and the absolute speed.

Abstract: A bus system for use with addressable memory has a global bus of uni-directional signal lines. The global bus has a first end and a second end. A master device transmits data to and receives data from the global bus. First and second global bus terminators are coupled to the first and second ends of the global bus, respectively. One or more subsystems are connected in parallel to each other and to the master device via the global bus. Each subsystem includes a local bus, one or more slave devices coupled to the local bus, a write buffer that receives incoming signals from the master device via the global bus and transmits the incoming signals to the one or more slave devices via the local bus, and a read buffer that receives outgoing signals from the one or more slave devices via the local bus and transmits the outgoing signals to the master device via the global bus.

Abstract: In order to reduce confusion about a displayed absolute altitude and to enhance reliability and correctness, the present invention provides a display method of an altitude display apparatus for displaying an absolute altitude wherein an initial atmospheric pressure is detected, and an absolute altitude is calculated and displayed based on a pressure difference between the initial atmospheric pressure and a current atmospheric pressure if the detected initial atmospheric pressure is larger than a predetermined atmospheric pressure.