Abstract: The present invention relates to the field of biotechnology and genetic engineering and in particular a novel nucleotide sequence which codes for a streptokinase, as well as the recombinant DNA obtained therefrom which is used for the transformation of various host organisms.The present invention is based on the isolation of a new gene which codes for streptokinase from Streptococcus equisimilis of type C (strain ATCC-9542) and the cloning and expression thereof in prokaryotic (E. coli) and eukaryotic (Pichia pastoris) hosts, for which it includes the vehicles of expression which contain the genetic sequences of said gene, as well as the microorganisms transformed with these vectors capable of producing streptokinase.The protein obtained thereby can be used in clinical medicine as a therapeutic agent, in the treatment of disorders such as thromboembolic obstructions including coronary thrombosis.
Type:
Grant
Filed:
May 22, 1991
Date of Patent:
March 22, 1994
Assignee:
Centro de Ingenieria Genetica y Biotecnologia
Inventors:
Mario P. E. Garcia, Aimee P. Felipe, Roger R. Chaplen, Ricardo S. Doce, Luciano F. H. Marrero, Pedro R. Collazo, Anaisel C. Ramirez, Emilio A. M. Munoz, Walfrido B. Martinez, Magalys C. Somavilla, Alicia P. Fernandez, Jose d. d. Garcia, Luis S. H. Martinez
Abstract: A technique for use in, illustratively, a transform coder for imparting robustness against burst (or similar) errors to data patterns, such as illustratively two-dimensional image data, that exhibit local redundancy. Robustness is provided, in the case of images, by passing localized (blocked) image data, i.e. either pixel values or transformed, illustratively discrete cosine transform (DCT), image coefficient values therefor, through a global block transform, such as a global block Hadamard transform, prior to compression coding in order to produce "holographic-like" compressed data for subsequent transmission and/or storage. Specifically, globally transforming an image in this fashion effectively spreads (diffuses) the image data in each block of pixels in that image or in the transform coefficients therefor in a regularly ordered pre-defined global manner throughout the entire image to create what is, in effect, intentionally "smeared" image data.
Type:
Grant
Filed:
April 10, 1991
Date of Patent:
March 8, 1994
Assignee:
International Business Machines Corporation
Inventors:
Ephraim Feig, William B. Pennebaker, Jr., Keith S. Pennington
Abstract: Apparatus and a method for generating a multi-level halftone image (104) from a digitally sampled continuous tone image (101). The apparatus includes a control circuit (140), a preference matrix (150) having as its matrix elements addresses of a plurality of look-up tables (160) and a plurality of look-up tables (160) in the form of a look-up table stack (155). Generally, the control circuit (140) instructs the preference matrix (150) to select a look-up table (160) from the look-up table stack (155) in a pre-defined manner. The selected table (160) is used to convert an intensity value (102) into a multi-level pixel value (106) in the halftone image (104). To accomplish the conversion, each look-up table (160) contains a quantized one-dimensional transfer function (165') having as an input the magnitude of the intensity value (102). The magnitude is mapped into an output level by the transfer function as one of a plurality of available levels.
Abstract: The present invention is concerned with a method for the isolation of a nucleotide sequence which codes for a protein having a molecular weight of about 64,000 daltons, which is located on the outer membrane of N. meningitidis, as well as with the recombinant DNA obtained therefrom, which is used for the transformation of a host microorganism. The technical object pursued with the invention is the identification of a nucleotide sequence coding for a highly conserved and common protein for the majority of pathogenic Neisseria strains, the production of this protein with a high level of purity and in commercially useful amounts using the recombinant way, so that it can be used in diagnostic methods and vaccine preparations with a broad immunoprotection spectrum.
Type:
Grant
Filed:
September 5, 1991
Date of Patent:
February 15, 1994
Assignee:
Centro de Ingenieria Genetica y Biotecnologia
Inventors:
Silva Rodriquez, Selman H. Sosa, Guillen Nieto, Saturnino H. Martinez, Julio R. F. Maso, Lidia I. N. Perez, Juan M. Grillo, Vivian M. Cordova, Sonia G. Blanco, Beatriz T. Santos, Jesus A. del Valle Rosales, Evelin C. Menendez, Anabel A. Acosta, Edelgis C. Rodriquez, Silian C. Leon, Alexis M. Lasa
Abstract: Circuitry for transforming a transfer function characteristic into a specified input impedance includes: a hybrid arrangement having input, transmit, receive and balance ports; and a two-port network connected from the transmit to receive ports of the hybrid. The relation between the input impedance at the input port to the network transfer function is expressible as a function of the actual impedance seen looking into the two-wire input port of the hybrid, and the desired impedance to be simulated.
Abstract: Sixty-four regions are established on an image sensed picture from a video camera, where each evaluating value r.sub.ij, b.sub.ij, and y.sub.ij is obtained by averaging color difference signals R-Y and B-Y and luminance signal Y obtained from an image sensed signal over 1 field period for each region. When determination is made that luminance evaluating value y.sub.ij out of these evaluating values exceeds a predetermined value, the color evaluating values of the corresponding region are attenuated by a predetermined amount, in response to gain control signals derived from the color difference signals. As a result, the effect of an object of high luminance towards white balance adjustment is reduced.
Abstract: Apparatus, and accompanying methods for use therein, for translating virtual page addresses in one address space, e.g. virtual, to page addresses in a second address space, e.g. real, and specifically for increasing the speed of such translations by translating multiple contiguous virtual page addresses upon the occurrence of a miss in a translation lookaside buffer (TLB). In response to a TLB miss, the address of each virtual page in a pre-defined block of, e.g. four, contiguous virtual pages, is separately translated through segment and/or page table lookup operations to yield corresponding page frame addresses. The virtual and corresponding page frame addresses for this block are then stored within a single TLB entry.
Type:
Grant
Filed:
May 24, 1990
Date of Patent:
January 25, 1994
Assignee:
International Business Machines Corporation
Abstract: Method and apparatus for cleaning a storage or transport tank by spraying a cleaning agent against the interior wall using at least one spray nozzle, said nozzle making a rotating movement in a plane, while said plane is simultaneously revolved around an axis which makes an angle with the axis of rotation of the nozzle, the point of impingement of a jet cleaning agent delivered by the nozzle describing a track over the interior wall of the container, said track passing a plurality of times a closed circumferential line on the wall of the tank, which line is chosen as a reference, wherein the nozzle or each nozzle is so controlled that passages of the impingement track that is being described substantially occur in the greatest as yet unintersected portion of said circumferential line, which portion is located between earlier points of intersection of the impingement track and said circumferential line namely, at distances from said earlier points of intersection which substantially bear a ratio of 1:(1/2.
Abstract: A MUSE sound decoder detects a broadcasting/non-broadcasting identification flag included in the control signals in an applied MUSE signal to determine whether the MUSE signal is broadcasted or non-broadcasted. The MUSE sound decoder further includes a frame synchronization protection circuit for protecting frame synchronization over a predetermined frame synchronization protection time period when the frame synchronization pattern is not detected at the proper timing. The frame synchronization protection circuit sets the frame synchronization protection time period to a long time period when the MUSE signal is determined to be a broadcasted MUSE signal, and to a short time period when the MUSE signal is determined to be a non-broadcasted MUSE signal. Appropriate muting of a sound signal can be carried out according to the condition of the received MUSE signal.
Abstract: The invention relates to the field of nutrition and the sugar industry and presents a method and apparatus for producing glucose-fructose liquors on an industrial scale from sugar or liquors thereof. The invention uses reactors packed with a catalyst with high hydrolytic activity, and these are installed within a sugar refining factory or in an industry which dissolves it, such that glucose-fructose syrup is produced in a single operation by a continuous flow of the sugar liquor. High levels of hydrolysis may be attained by modification of the residence time. The process of hydrolysis of the sugar does not significantly alter the color of the solution. The product obtained on an industrial scale can be used both in the food industry and in the pharmaceutical industry.
Type:
Grant
Filed:
May 6, 1992
Date of Patent:
December 14, 1993
Assignee:
Centro de Ingenieria Genetica y Biotecnologia
Abstract: A printed circuit board for connecting, in n different ways, connection pins of an integrated circuit mounted in a dual-in-line (DIL) package to printed circuitry on the printed circuit board, where n is an integer .gtoreq.2. The printed circuit board comprises contact holes which are electrically connected to the printed circuitry and are each suitable for receiving a connection pin of the DIL package. To avoid the use of so-called jumper blocks for the different ways of connection, the contact holes form a matrix of p columns and q rows, with p being at least equal to the number of connection pins on a long side of the DIL package, q being at least equal to 2n, and the distance between successive rows of contact holes being such that n-1 rows of free contact holes are located between the rows of connection pins on the opposite long sides of the DIL package when these rows of connection pins have been mounted in two rows of contact holes.
Abstract: An application, executing on a first processing element in a MP system without an asymmetric feature, issues an instruction requiring that feature to complete. A program check interruption gives control to interrupt handlers, which create a high-priority, non-preemptable work unit control block and enters the dispatcher to enqueue the work unit on a processor-related queue associated with a second processing element having the asymmetric feature. When the dispatcher executes in the second processing element, it executes the non-preemptable work unit, which transfers control to the application at the point of interruption. Subsequently the application has only whatever processor affinity obtained prior to the program check.
Abstract: Exposure control apparatus, and various accompanying methods, for use in a photographic camera for improving the overall quality of photographed images, i.e. increasing the number of acceptable and higher quality images, that are produced by the camera for user-selected non-standard display sizes and/or different focal length photographing modes over that obtainable by adherence to ISO/ANSI exposure standards. The quality improvement is attained through user selection of a desired display size and/or focal length photographing mode for each image to be captured followed by an optimization, for that size and mode, of various photographic exposure parameters (exposure settings and, where appropriate, flash parameters). The invention violates the ISO/ANSI exposure standards where necessary to improve image quality, for the desired display size and focal length photographing mode, beyond that which would result from adherence to these standards.
Abstract: The present invention relates to a magnetic recording/reproduction device (such as VTRs and DATs). A cue signal becomes a mark of its position for automatic operation and automatic editing and is to be recorded in advance with the contents of a program. The frequency of the cue signal is selected within the audio signal band. On an auxiliary audio track (24) in a magnetic tape (21), an audio signal as well as a cue signal is recorded in time division manner. Determination is made of a correct cue signal by providing a mute period before the cue signal recording region and detecting a cue signal by a filter after the detection of a mute period. The frequency ratio of the cue signal to control signal (CTL) is determined by a counter (6) with a CPU (7) being used to identify a cue signal in response to the frequency ratio. By dividing the reproduced signal into blocks, the cue signal is detected from the cycle number distribution of the frequency.
Abstract: The present invention relates to an automatic focusing camera in which an automatic focusing operation is performed by detecting a high frequency component of a video signal obtained from an image sensor device at every predetermined period, and has a theme that the automatic focusing operation can be performed within an extremely short time. To this end, by a search means, a focus lens is moved from a first position to a second position with relatively coarse steps to obtain a focus evaluating value for each step. In a first method, after the focus lens is moved to the vicinity of a lens position corresponding to a maximum focus evaluating value obtained by the search means, the focus lens is moved with small incremental steps to find a true maximum focus evaluating value.
Abstract: Apparatus and accompanying methods for inclusion in a Coriolis meter that substantially eliminate temperature induced measurement errors which might otherwise be produced by performance differences existing between the separate input channels contained in the meter. Specifically, two pairs of input channels are used in the meter. In operation, the meter repetitively measures the internal phase delay of each of these pairs and then subtracts the delay associated with each pair from actual flow based measurement data subsequently obtained therefrom. While one channel pair is measuring actual flow, the other channel pair is measuring its internal phase delay, with the channels being continuously cycled between these functions.
Abstract: Method and device for enciphering data words of a word width of n bits, in particular data words to be written in a computer storage, wherein a product cipher circuit comprises alternately one from a plurality of permutation boxes with n inputs and n outputs and one from a plurality of substitution boxes with n inputs and n outputs, each of these boxes being under the control of a specific part of an m-bits key. In the product cipher circuit the data words are consecutively enciphered in whole and the enciphering device can be regarded as a delay line. The data words to be enciphered can be combined with coding words which depend on the specific sector of the computer storage, in particular a hard storage disk unit, where the data words are stored. The sector-specific coding words and/or the m-bits key can be combined with a key to be entered by a user.
Abstract: Apparatus and accompanying methods for inclusion in a Coriolis meter for measuring and updating a mechanical zero value for the meter using only zero flow measurements that have a sufficiently low noise content. Specifically, the meter produces zero flow measurements while no fluid is flowing through the meter. If a standard deviation of these zero flow measurements is less than a pre-defined limit value, then a resulting mechanical zero value, based on those measurements, is subsequently subtracted from the flow based measurement data.
Abstract: A digital quadrature modulator having a first storage device for receiving an I phase component of a digital baseband signal and digital carrier phase information as a higher order address and a lower order address, respectively, and a second storage device for receiving a Q phase component of the digital baseband signal and the digital carrier phase information as a higher order address and a lower order address, respectively. The carrier phases of the carrier phase information applied to the first and the second storage devices are out of phase from each other by .pi./2. The first storage device outputs a multiplication result of the I phase component and a carrier signal and the second storage device outputs a multiplication result of the Q phase component and the carrier signal based on these addresses. These outputs are added to each other in a digital manner and then converted into an analog signal which is supplied as a modulated signal.