Abstract: A method for the assay of N samples each containing a compound to be tested, comprises providing N reaction vessels each containing a population of carrier beads and other reagents for performing the assay, where N is at least 2 e.g. 80-4000. Each population of carrier beads is distinguishable from every other population. After adding the samples to the reaction vessels and performing the assays, the contents of all the reaction vessels are mixed and subjected to analysis by flow cytometry. By means of flow cytometry, each carrier bead is rapidly analysed to identify its population and also to determine the presence or concentration or biological activity of the compound to be tested.
Abstract: Exploitation of suitably functionalized heterocyclic molecules, in the design and synthesis of Fluorescence Resonance Energy Transfer (FRET) cassettes and their corresponding dideoxynucleotide terminators culminated into efficient reagents for DNA sequencing. Additionally, these FRET cassettes/terminators, of the present invention, derived from different classes of heterocyclic systems have high potential to be used for general labelling of biological molecules to generate highly sensitized signals. Their preparation, energy transfer efficiency, and use as labels, specifically, in DNA sequencing reactions is disclosed.
Abstract: A method for producing IgG from plasma for medical applications, comprising at least: (i′) removal of albumin resulting in an IgG fraction, (ii′) purifying IgG from an IgG fraction, which is derived from the IgG fraction obtained in step (i′), by adsorbing IgG to a cation exchanger and collecting the adsorbed IgG fraction, and (iii′) virus inactivation in an IgG fraction derived from the IgG fraction collected in step (ii′). The method is characterized in; (I) concentrating the IgG fraction obtained in step (i′), (II) adjusting pH to 4±0.1 in the IgG fraction released from the cation exchanger in step (ii′), and preferably maintaining the pH below 6.0 during the remaining steps of the method; and (III) carrying out the virus inactivation (step iii′) by using chemicals at a temperature of 30° C.±2° C. for at least 4 hours. Anticomplementary activity is typically below 1 CH50/mg immunoglobulin.
Abstract: The present invention relates to analysis devices having means (3, 5, 7) for producing a plurality of ion beams of samples substantially simultaneously; mass separating means for individually mass separating each ion beam in parallel and detecting means (131-13n) for detecting said mass separated ion beams substantially simultaneously, and to methods for using such devices.
Abstract: Disclosed is a non-fluorescent cyanine dye that may be used as an acceptor in fluorescence energy transfer assays involving the detection of binding and/or cleavage events in reactions involving biological molecules, and assay methods utilising such dyes.
February 15, 2001
Date of Patent:
December 7, 2004
Amersham Biosciences UK Limited
Alan L. Hamilton, Martyn N. Birch, Malcolm J. Hatcher, Nigel Bosworth, Brian Scott
Abstract: Laser pulses from laser head 12 pass through objective lens 16 to a focal spot in sample spot 17. If a pulse hits a fluorophore molecule in the focal spot a fluorescence photon is emitted which is collected by lens 16, reflected by dichroic mirror 15 through filter 20, which blocks photons of other wavelengths, to single photon counting photomultiplier unit 21. On detecting a photon, photomultiplier unit 21 generates an output pulse which is coupled, with a short delay, by pulse controller 30 to driver circuit 24 which causes laser head 12 to generate another laser pulse. Thus the interval between the laser pulses is substantially equal to the time between excitation of, and fluorescence emission by, the fluorophore molecule. Computer 23 coupled to photomultiplier unit 21 and driver circuit 24 determines the decay time by measurement of a number of fluorescence events.
Abstract: A novel method for separating a target substance, for example, metal ion, drug or biological component is provided. According to the method, the surface of a packing undergoes a chemical or physical environmental change under a physical stimulus so that the interaction of a substance interacting with the target substance is reversibly changed in an aqueous solution, thus effecting separation.
May 9, 2003
Date of Patent:
October 19, 2004
Japan Chemical Innovation Institute, The Agency of Industrial Science and Technology of the
Ministry of International Trade and Industry
Abstract: Porous hollow polymer fiber membranes having convoluted inside and/or outside surfaces, as well as filter devices comprising a plurality of the hollow fiber membranes, the devices preferably being arranged to direct fluid flow from the inside surface of the membranes to the outside surface, methods of making the membranes, and methods of using the filter devices, are disclosed.
Abstract: A micro-channel plate (MCP) detector system (30) comprising a MCP detector, a data acquisition unit (20), wherein the detector comprises a first and a second MCP electron multiplier (12, 14), one or more anodes (16) connected to the data acquisition unit (20) and a gate electrode (32) disposed between the first and the second MCP electron multiplier (12, 14), wherein the detector system further comprises a data storage unit (36) and a gain control unit (34) which is connected to the gate electrode (32) and to the data storage unit (36), wherein a pilot spectrum is stored in the data storage unit (36), and wherein the gain control unit (34) is arranged to read the pilot spectrum from the data storage unit (36), and to control the potential on the gate electrode (32) as a function of m/z or time in response to said pilot spectrum, such that the transmission of electrons to the second MCP electron multiplier (14) is lowered when abundant protein ions appear, whereby a high sensitivity is maintained during the re
Abstract: This invention relates to the improvement of arrays of porous polymer pads on solid supports used in biological assays. The invention involves freeze drying the porous polymer pads to increase pore size. The increased pore size results in an enhanced ability of the porous polymer pads to bind specific binding substances such as DNA, RNA and polypeptides.
Abstract: The present invention relates to a method, a measuring cell and a system for measuring very small heat changes in a sample. The system comprises a measuring cell 16 for containing the sample during the measurement process, at least one electromagnetic radiation unit 14 for radiating one or several samples with modulated monochromatic or polychromatic radiation 46 inside said measuring cell 16. Said measuring cell 16 comprises at least one acoustic transducer 22 for generating a first output signal V(t) and at least one heat measuring device 24 for generating a second output signal T(t). Both signals are connectable to a combining unit 18 that generates an information signal by means of a reference signal f(t). Said information signal is connectable to a signal processing unit 20 for determining at least one relevant reaction parameter as a function of the measured heat change.
Abstract: Disclosed is an apparatus for use in the measurement of the heat generated in a chemical or biochemical reaction, by detecting and measuring a change in the conformation of a polymer transducer responsive to a heat change, the transducer being bound to the surface of the waveguides of an interferometer. The conformational change is detected by optical means and is compared with a control. The polymer transducer may be selected from an organic polymer or a biological macromolecule.
Abstract: An inlet device for a reactor vessel (5) intended for a separation method comprising retaining a substance on a separation medium from a liquid passing through the reactor vessel (5) which contains the separation medium. The device comprises (a) a distributor block (7) enabling a liquid flow to pass through the block into the reactor vessel (5), (b) an inlet block (3), (c) a distribution chamber (1) defined by a space between the distributor block (7) and the outlet side (11) of the inlet block (3), (d) at least one conduit (I; 13a,13b . . . ) passing through the inlet block (3) from the inlet side (10) to the outlet side (11) of said inlet block (3) and ending in the distribution chamber (1), said at least one conduit being able to distribute liquid into the distribution chamber (1), (e) a gross liquid flow direction going perpendicular to the plane of the interface between the distribution block (7) and the distribution chamber (1).
May 6, 2003
Date of Patent:
June 15, 2004
Amersham Biosciences AB
Joachim Walter, Uwe Striffler, Jan Feuser
Abstract: The monoisotopic mass peak of an experimentally obtained mass spectrum (262) for a sample molecule is determined using a cross correlation method. A model spectrum (261), having a known monoisotopic peak position, is created based on knowledge of the sample molecule. A set of correlation values are calculated from intensity values IMODEL, IEXP for a selected mutual alignment between the spectra. A cross correlation analysis is used to find the best agreement between the model and the experimental mass spectrum, represented by a best value of a quality factor. The position of the monoisotopic peak of the model spectrum showing the best agreement with the experimental mass spectrum is selected as the best approximation of the monoisotopic peak of the experimental spectrum. Knowing the charge state of the analysed section of the mass spectrum it is possible to determine the monoisotopic mass of the sample molecule.
Abstract: The present invention provides a single analog-to-digital converter system which is capable of converting an input signal having a high dynamic range into a digital signal which is within the range of the analog-to-digital converter. Additionally, the present invention provides an analog-to-digital converter which is capable of converting an input signal having a high dynamic range into a digital signal by controlling the amplification of the signal to achieve the optimal gain that falls within the range of the analog-to-digital converter.
Abstract: The present invention relates to a method for the quantitative release of natural or recombinant proteins, polypeptides (thermostable immunoligands) able to bind to the Fc-part of immunoglobulins (antibodies, in particular of the IgG class and primarily becoming bound outside the paratope) from complexes in various sample matrixes in order to make these released natural or recombinant proteins, polypeptides or peptides quantitatively available in immunochemical assays and to keep them quantitatively available. The method is characterized by mixing the sample with reagent compound that is able to bind non-specifically to immunoglobulins, and thereafter subjecting the sample to a heat treatment step followed by a cooling step.
Abstract: A liquid chromatographic process performed at least partly in a fluidised bed contained in a vessel and comprising particle fluidised by an upwardly directed liquid flow, said process comprising (a) a capture step in which one or more compounds of a sample are captured by the particles and (b) a wash and/or releasing step in which the particles is in form of a fluidised bed through which a liquid flow is passing. The characteristic feature is that the liquid (liquid 1) used in the wash or the releasing step (step 1) is immediately followed by a liquid (liquid 2, step 2) having a higher density than liquid 1 while maintaining the bed in a fluidised state. A liquid chromatographic process having an actual sequence of steps comprising (c) at least a capture step in which a sample deriving from an animal is bound to the particles and (d) two consecutive steps (step 1 and step 2) in which the bed is fluidised by a liquid flow passing through said vessel.
Abstract: A method for the removal of a substance carrying a negative charge and being present in an aqueous liquid (I). The method comprises the steps of: (i) contacting the liquid with a matrix carrying a plurality of ligands comprising a positively charged structure and a hydrophobic structure, and (ii) desorbing the substance.
September 16, 2002
Date of Patent:
March 9, 2004
Amersham Biosciences AB
Bo-Lennart Johansson, Mikael Andersson, Jan Gustavsson, Makonnen Belew, Jean-Luc Maloisel
Abstract: A method of assaying for an analyte which occurs at least partially bound as a complex with its soluble receptor or binding protein, the method comprising the steps of:
i) mixing a biological fluid serum sample containing the analyte to be determined with a detergent for dissociating said complex,
ii) mixing the sample from step i) with reagents, including a specific binding partner of the analyte for binding to the analyte, for performing a specific binding assay for the analyte,
iii) and mixing the sample from step i) with a sequestrant for the detergent, whereby the binding of step ii) is performed in the presence of the sequestrant.