Patents Represented by Attorney Stephen G. Ryan
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Patent number: 6518040Abstract: An in vitro method for production of a protein (polypeptide), characterised in that the mRNA encoding the protein is translated when bound to a solid phase. By allowing for a pause at a terminating stop codon and addition of release factor the final proteon may be obtained in almost pure form. By allowing for pauses and restart at internal codons and replacement of the amino acid mixture used before a pausing with an amino acid mixture that differs with resect to labelling, region-labelled polypeptides/proteins can be accomplished. A labelled polypeptide/protein, characterised in that its amino acid sequence contains one or more regions of two or more labelled amino acid residues in sequence. In a subaspect, the polypeptide/protein has at least one amino acid that is occurring twice and this amino acid differs in labelling at at least two positions.Type: GrantFiled: June 22, 2000Date of Patent: February 11, 2003Inventors: Mans Ehrenberg, Michail Pavlov
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Patent number: 6495017Abstract: An improved system for running electrophoresis gels “face up” is described. The system includes a single gel capacity strip holder, two electrodes, a sample cup, and a cover. The system provides a means of separating basic proteins on any length gel strip and the cup provides a user-friendly means of successfully putting the proteins on the gel face.Type: GrantFiled: April 10, 2000Date of Patent: December 17, 2002Assignee: Amersham Pharmacia Biotech, Inc.Inventors: Mohammed Rezaul Islam, Robert S. Jetter
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Patent number: 6479267Abstract: A purified recombinant thermostable DNA polymerase polymerase which exhibits at least about 80% activity at salt concentations of 50 mM and greater, at least about 70% activity at salt concentrations of 25 mM and greater, and having a processivity of about 30 nucleotides per binding event. An isolated nucleic acid that encodes the thermostable DNA polymerase, as well as a recombinant DNA vector comprising the nucleic acid and a recombinant host cell transformed with the vector, are also disclosed. A method of sequencing DNA using the DNA polymerase as well as a kit for sequencing DNA is also disclosed.Type: GrantFiled: June 17, 1999Date of Patent: November 12, 2002Assignee: Amersham Pharmacia Biotech, Inc.Inventors: Maria Cuozzo Davis, Carl W. Fuller, Lin Huang, Joseph Anthony Mamone
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Patent number: 6478042Abstract: A sliding valve having a flow line on which there may be placed a module having flow channel that can be connected and disconnected. The valve avoids the problem of liquid remaining in the flow channel of a first valve component by providing flow channel intersecting segment which intersects both components of the sliding valve so that in a module disconnecting orientation, each valve component defines separate segments of the flow channel while in the connected orientation, the valve components define a single flow channel. The construction allows for liquid flow through the valve independent of the module being in connected or disconnected position.Type: GrantFiled: December 4, 2000Date of Patent: November 12, 2002Assignee: Amersham Pharmacia Biotech ABInventor: Jan Bergström
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Patent number: 6468409Abstract: An apparatus for detecting fluorophore labelled substances in an electrophoretic separation medium comprises illuminating means (1) for illuminating the fluorophore labelled substances (8), detecting means (7) for detecting fluorescence emitted by said fluorophore labelled substances upon illumination, and illumination varying means (9) for varying the illumination of the fluorophore labelled substances (8) to vary the intensity of the fluorescence.Type: GrantFiled: September 13, 2000Date of Patent: October 22, 2002Assignee: Amersham Biosciences ABInventors: Allan Asp, Bengt-Goran Andersson
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Patent number: 6458966Abstract: Various classes of dyes are provided having acid, ester or amide groups for covalent linking to biomolecules. The dyes may be prepared by use of a compound of formula (I) where R1 comprises a linker and a carboxy including acid, salt, ester including N-hydroxysuccinimide, activated ester or amide group; R2, R3, R4 and R5 are H, C1-C10 alkyl or aralkyl or a group to modify solubility or electronic or spectral properties or a functional linking group: or R4-R5 and/or R2-R4 and/or R2-R3 are linked to form an extended ring system; and R6 is H or CHO or NO.Type: GrantFiled: April 24, 2000Date of Patent: October 1, 2002Assignee: Nycomed Amersham plcInventors: John Griffiths, John Mama, Valerie Millar, Mark Briggs, Alan Hamilton
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Patent number: 6454950Abstract: A method for the separation of a predetermined compound comprising i) partitioning the compound in a two-phase (system A) in which at least one of the phases is rich (phase 1) and the other is poor (phase 2) in a thermoseparating polymer (I), ii) collecting one phase (phase 1 or phase 2) containing said compound, and iii if desired, further working up said compound from the phase collected in step ii, characterized a) in that polymer (I) is a micell-forming thermoseparating polymer, and b) in that phase 2 possibly contains at least one agent that is cloud point-decreasing for thermoseparating polymer (I), such as a polymer (II) that is incompatible with polymer (I) or a salt.Type: GrantFiled: October 3, 2001Date of Patent: September 24, 2002Assignee: Amersham Pharmacia Biotech ABInventors: Folke Tjerneld, Josefine Persson, Hans Olof Johansson
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Patent number: 6450218Abstract: A fraction collector (1) for dispensing liquid from a liquid source into receptacles (23) carried on a rotatable turntable (6), wherein the turntable is connected to the fraction collector casing (5) via a support arm (7) such that the turntable is rotatably mounted at one end of said support arm, and said support arm at its other end being rotatably mounted to the fraction collector casing. Thereby it is possible to align any position of the turntable under a dispensing tube (4) to dispense a liquid into a receptacle placed anywhere on the turntable.Type: GrantFiled: February 2, 2001Date of Patent: September 17, 2002Assignee: Amersham Pharmacia Biotech ABInventor: Lars Andersson
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Patent number: 6448010Abstract: A method for detecting a mutation in a target nucleic acid sequence that comprises attaching oligonucleotide primers to a substrate, wherein the oligonucleotide primers have a sequence that is complementary to the target nucleic acid sequence, and wherein the oligonucleotide primers are grouped according to the identity of the first base which would be expected to be added to the primer through the process of primer extension; hybridizing to the oligonucleotide primers a sample nucleic acid sequence which possibly contains a mutation; extending each oligonucleotide primer by one base using a reaction mixture comprising labeled ddNTPs; and detecting a mutation in the sample nucleic acid sequence by detecting the presence of a labeled ddNTP which does not correspond to the identity of the base expected to be added to the primer through the process of primer extension. Mutations may be detected in mRNA or DNA.Type: GrantFiled: October 4, 2000Date of Patent: September 10, 2002Assignee: Amersham Pharmacia Biotech, Inc.Inventor: Xiaodong Zhao
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Patent number: 6444193Abstract: The invention provides a process for the preparation of gas-containing vesicles wherein a mixture of gas, liquid and vesicle membrane forming material is passed through a zone in which it is subject to shear forces exerted by surfaces moving relative to each other at a speed of at least 20 m/s.Type: GrantFiled: February 14, 2000Date of Patent: September 3, 2002Assignee: Nycomed Imaging ASInventors: Tore Omtveit, Bjorn Haugseter, Tony Pete
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Patent number: 6441235Abstract: A process for preparing 5-amino-N,N′-bis(R)isophthalamides, where A is 2,3-dihydroxypropyl or 1,3-dihydroxyisopropyl, useful as intermediates in preparing iodinated diagnostic agents, which comprises reacting a di-lower-alkyl 5-nitroisophthalate with a compound of the formula RNH2 in the presence of a basic catalyst and without isolating the intermediate 5-nitro-N,N′-bis(R)isophthalamide, catalytically hydrogenating the latter.Type: GrantFiled: May 14, 2001Date of Patent: August 27, 2002Assignee: Amersha, Health ASInventors: Edward David Parady, Karl Olaf Gelotte
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Patent number: 6428707Abstract: A method for adsorption of a substance from a liquid sample on a fluidized bead or stirred suspension, in which the beads used comprise a base matrix and exhibit a structure having affinity to the substance, characterized in that the structure is covalently bound to the base matrix via an extender. Populations of beads in which the beads contain a filler incorporated in a base matrix and an extender are also described.Type: GrantFiled: March 13, 2000Date of Patent: August 6, 2002Assignee: Amersham Pharmacia Biotech ABInventors: Hans Berg, Hasse Hansson, Lennart Kagedal
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Patent number: 6427731Abstract: This invention is directed to a cassette chemical immobilization and treatment system and method for enabling the performance of various complex chemistries with minimal human intervention, near-zero dead volume, and flow-through protocols pursuant to a predetermined instruction set encoded on a multiple-address chemical treatment cassette assembly. The cassette assembly comprises a plurality of analyte sample columns (“mini-columns”), reagent wells containeing pre-packaged reagents including powdered, micoencapsulated, liquid or lyophillized reagents, analyte loading funnels, alignment means for the analyte sample columns, and, a machine readable instruction code Bet for identifying a chemical treatment protocol. The mini-columns are improved columns having high pressure interface capability to permit direct insertion of the mini-column into high-pressure solvent line for use as a support column for HPLC analysis.Type: GrantFiled: December 13, 1999Date of Patent: August 6, 2002Assignee: Amersham Pharmacia Biotech ABInventor: Marcus J. Horn
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Patent number: 6428479Abstract: Prostate abnormalities such as cancer may be detected by ultrasonic determination of the in-flow kinetics of contrast agent-containing blood in the prostate and/or by observation of disease-related asymmetries in the spoke-like vascular pattern of the prostate.Type: GrantFiled: June 19, 2000Date of Patent: August 6, 2002Assignee: Nycomed Imaging ASInventors: Anne Kirsti Aksnes, Morten Eriksen, Else Kruger Hagen, Audun Tornes
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Patent number: 6426315Abstract: Process for, in layers of a porous matrix, exhibiting the groups A, introducing a functionality using a reagent I, introducing the functionality by a reaction with the groups A. The characterizing feature is that the matrix is contacted with a functional deficiency of reagent I and that conditions and reagent I are chosen so that the reaction between reagent I and the groups A is more rapid than diffusion of reagent I in the matrix.Type: GrantFiled: March 14, 2000Date of Patent: July 30, 2002Assignee: Amersham Pharmacia Biotech ABInventors: Jan Bergstrom, Rolf Berglund, Lennart Soderberg
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Patent number: 6420028Abstract: A polymer article having structure (I), where P comprises the basic polymer and groups —L—CH(—X(R1)p)—CH2(—Y(R2)p); L is a part of a pending group utilized for introducing —X(R1)p and —Y(R2)p; X and Y are halogen, N, S and O; R1 is hydrogen, alkyl, acyl or R2 when X is N, O or S; R2 is hydrogen, alkyl, alkylaryl or arylalkyl in which the alkyl part may contain 1-18 carbons, or —R3(—NH—CR4═O)q or —R3(—NH2)q, —CR4═O or poly alkyloxy that may have been terminally acylated or alkylated; p is an integer 0-3, with the provisos a) p depends on X being halogen, O, S or N, and b) that if several groups R2 are present they may be identical or different; R3 is alkyl, —O-alkyl, hydroxyalkyl, phenylalkyl, with up to 18 carbon atoms in the alkyl part, and R4 is C1-18 alkyl. q is an integer>O representing that one or more hydrogens in R3 may have been replaced with —NH2 or —NH—CR4═O.Type: GrantFiled: December 22, 1999Date of Patent: July 16, 2002Assignee: Amersham Pharmacia Biotech ABInventors: Steinar Hagen, Rune Ringberg, Geir Fonnum
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Patent number: 6411380Abstract: The present invention relates generally to a detection device for a spectrophotometer system, and in particular to a light detection device with an analogue input and a digital output. The object of the present invention is to provide a more accurate detection device, which requires substantially less space for accommodating the detection device in a spectrophotometer. This object is solved by using a successive approximation A/D converter (16) which has internal sample and hold circuits, and which heretofore has been used in the audio industry. This will increase the accuracy of the detection device and make it more resistant against disturbances. It will also decrease the space required for such detection device, down to one third compared to conventional detection devices.Type: GrantFiled: June 7, 1999Date of Patent: June 25, 2002Assignee: Amersham Pharmacia Biotech ABInventor: Björn Berg
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Patent number: 6409671Abstract: Local aberrations in the perfusion and/or compliance of vasculated tissue may be detected by contrast agent-enhanced ultrasound imaging procedures wherein a sequence of ultrasound image data is generated in respect of a region of interest in vasculated tissue, the image data is processed to generate waveforms representative of arterial pulsatility, and these waveforms are analyzed for variations such as phase distortions which are characteristic of local aberrations in tissue perfusion and/or compliance.Type: GrantFiled: June 19, 2000Date of Patent: June 25, 2002Assignee: Nycomed Imaging ASInventors: Morten Eriksen, Sigmund Frigstad
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Patent number: 6406680Abstract: This invention describes a new type of X-ray contrast agents. It has been found that iodinated alkenes containing one or several C═C double bonds substituted with electronic neutral substituents and iodine can be used as X-ray contrast agents. Also new iodoalkene compounds are described.Type: GrantFiled: September 6, 2000Date of Patent: June 18, 2002Assignee: Nycomed Imaging ASInventor: Hanno Priebe