Abstract: An acidic phospholipase is obtained from a strain of the genus Hyphozyma. It is able to hydrolyze both fatty acyl groups in intact phospholipid. Advantageously, it has no lipase activity and is active at very low pH; these properties make it very suitable for use in oil degumming, as enzymatic and alkaline hydrolysis (saponification) of the oil can both be suppressed. The phospholipase is not membrane bound, making it suitable for commercial production and purification.
Type:
Grant
Filed:
April 20, 1999
Date of Patent:
October 3, 2000
Assignee:
Novo Nordisk A/S
Inventors:
Miyoko Hasida, Noriko Tsutsumi, Torben Halkier, Mary Ann Stringer
Abstract: Divalent cation crystals of human growth factor (hGH) or derivatives thereof, and pharmaceutical preparations comprising divalent cation crystals of hGH. In specific embodiments, the divalent cation is Zn++ and the molar ration Zn++ and hGH is about 0.2 to about 10.
Abstract: The present invention relates to variants of a Family 7 cellulase comprising one or more mutations of the amino acid sequence of the cellulase in which each mutation is independently a substitution of an amino acid residue with another amino acid or an insertion of at least one amino acid residues, wherein the variant has a modified H-bonding potential and hydrophobic interaction relative to the cellulase. The present invention also relates to the use of the cellulase variants in detergent compositions, especially for soil removal or color clarification or preventing backstaining; in fabric softeners; for bio-polishing of textiles; for drainage improvement of paper pulp; for deinking of old paper; for plant degradation.
Type:
Grant
Filed:
September 9, 1996
Date of Patent:
September 12, 2000
Assignee:
Novo Nordisk A/S
Inventors:
Martin Schulein, Peter Rosholm, Jack Bech Nielsen, Svend Aage Hansen, Claus von der Osten
Abstract: This invention is a protease mutant wherein said mutant substitutes the naturally occurring tyrosine amino acids for other amino acids at positions 91, 167, 171, 192, 209, 214, and 263. The protease mutants are used in detergent compositions.
Type:
Grant
Filed:
December 13, 1999
Date of Patent:
August 29, 2000
Assignee:
Novo Nordisk A/S
Inventors:
Grethe Rasmussen, Egon Nielsen, Torben Halkier
Abstract: A method of improving properties of a dough and/or a baked product made from dough by adding an enzyme preparation to the dough and/or to any ingredient and/or to any mixture of the dough ingredients, in which the enzyme preparation comprises a lipase derived from Humicola, and a second enzyme which is an amylase or a xylanase.
Type:
Grant
Filed:
January 4, 1999
Date of Patent:
August 29, 2000
Assignee:
Novo Nordisk A/S
Inventors:
Tine Olesen, Joan Qi Si, Vahan Donelyan
Abstract: The present invention relates to protease variants, stabilized towards the inactivation caused by peroxidase systems, in which protease variants a naturally occurring tyrosine residue has been deleted or substituted with a different amino acid residue at one or more positions. The invention also relates to a method of stabilizing a protease towards the inactivation caused by peroxidase systems, and detergent compositions comprising a protease variant of the invention.
Type:
Grant
Filed:
May 7, 1997
Date of Patent:
July 11, 2000
Assignee:
Novo Nordisk A/S
Inventors:
Grethe Rasmussen, Egon Nielsen, Torben Halkier
Abstract: The present invention relates to the use of one or more oxidoreductases in combination with a mediator for the reduction of malodour. Malodour reducing compositions and products comprising such composition are also claimed.
Abstract: This invention relates to a process for the treatment of fabrics. More specifically the invention relates to a process for the treatment of fabrics, which process comprises treating the fabric at elevated temperatures with an effective amount of a thermostable lipolytic enzyme.
Type:
Grant
Filed:
January 16, 1998
Date of Patent:
June 20, 2000
Assignee:
Novo Nordisk A/S
Inventors:
Henrik Lund, Thomas Erik Nilsson, Tom Pickard
Abstract: A lipase variant of a parent lipase comprising a trypsin-like catalytic triad including an active serine located in a predominantly hydrophobic, elongated binding pocket of the lipase molecule and, located in a critical position of a lipid contact zone of the lipase structure, an amino acid residue different from an aromatic amino acid residue, which amino acid residue interacts with a lipid substrate at or during hydrolysis, in which lipase variant said amino acid residue has been replaced by an aromatic amino acid residue so as to confer to the variant an increased specific activity as compared to that of the parent lipase. The parent lipase may be a C. antarctica lipase A essentially free from other substances from C. antarctica, which comprises the amino acid sequence shown in SEQ ID No. 2, or a variant of said lipase which (1) has lipase activity, (2) reacts with an antibody reactive with at least one epitope of C. antarctica lipase A having th eamino acid sequence SEQ ID No.
Type:
Grant
Filed:
December 22, 1994
Date of Patent:
June 13, 2000
Assignee:
Novo Nordisk A/S
Inventors:
Allan Svendsen, Shamkant Anant Pathar, Michi Egel-Mitani, Kim Borch, Ib Groth Clausen, Mogens Trier Hansen
Abstract: The present invention relates to methods of constructing recombinant Bacillus strains encoding a polypeptide of interest, in which method a DNA construct comprising a DNA sequence encoding the polypeptide of interest is introduced into a recipient Bacillus cell by conjugation from a donor cell.
Type:
Grant
Filed:
July 22, 1998
Date of Patent:
May 23, 2000
Assignee:
Novo Nordisk A/S
Inventors:
Martin Tangney, Christian Hansen, Poul Erik Pedersen, Per Lina Jorgensen, Steen Troels Jorgensen
Abstract: A cholesterol esterase from Pseudomonas fragi FERM BP-1051 can be used to hydrolyze cholesterol ester. The enzyme can be used in cleaning compositions.
Abstract: A method of making an animal fodder stabilized against microbial degradation, by jet-cooking potato pulp at a temperature of between about 125.degree. C. and 140.degree. C. for between about 20 to 100 seconds, cooling the jet-cooked potato pulp, and adding a cell-wall degrading enzyme to the cooled potato pulp in order to enzymatically degrade the cell walls in the potato pulp, wherein a fodder stabilized against microbial degradation is produced.
Abstract: The present invention relates to a recombinant glucagonlike peptide-1 (GLP-1) receptor having the nucleotide sequence of SEQ ID NO:1 or SEQ ID NO:3, wherein the receptor polypeptide binds GLP-1 with a Kd of less than 100 nM.
Abstract: A method of treating wool, wool fibers or animal hair with a proteolytic enzyme and a transglutaminase. The described method results in improved shrink-resistance, handle, appearance, wettability, reduction of felting tendency, increased whiteness, reduction of pilling, improved softness, tensile strength retention, improved stretch, improved burst strength, and improved dyeing characteristics such as dye uptake and dye washfastness. Furthermore, relative to treatments with proteolytic enzymes alone (no transglutaminase), the described method results in reduced weight loss, reduced fiber damage, and improved strength.
Abstract: The present invention relates to Desulfurococcus amylopullulanase preparations and their use in producing sweeteners and ethanol from starch.
Abstract: A method for preparing a dough or a baked product produced from the dough, including adding to dough or dough ingredients an L-amino acid oxidase or a benzylamine oxidase in an amount effective to improve gluten strength, stickness, or rheological properties of dough, or specific volume of the baked product. Further includes are bread- or dough-improving compositions including an L-amino acid oxidase or a benzylamine oxidase.
Abstract: The present invention relates to a method of obtaining protein hydrolysates useful as flavoring agents. More specifically, the invention provides a method of obtaining from a proteinaceous sustrate a hydrolysate enriched in free glutamic acid and/or peptide bound glutamic acid residues, which method comprises the steps of subjecting the substrate to a deamidation process, and subjecting the substrate to the action of a specific acting proteolytic enzyme.
Abstract: Animal feed compositions and methods for treating one or more of soy, pea or rape-seed, or other material derived from Fabales or Cruciferaceae, with an enzyme having rhamnogalacturonase activity, wherein the enzyme having rhamnogalacturonase activity cleaves a rhamnogalacturonan backbone to produce rhamnose as a non-reducing end (RGase II) or cleaves a rhamnogalaturonan backbone to produce galacturonic acid as a non-reducing end.
Type:
Grant
Filed:
November 30, 1998
Date of Patent:
March 7, 2000
Assignee:
Novo Nordisk A/S
Inventors:
Lene Venke Kofod, Lene Nonboe Andersen, Henrik Dalb.o slashed.ge, Markus Sakari Kauppinen, Stephan Christgau, Hans Peter Heldt-Hansen, Claus Christophersen, Per Munk Nielsen, Alphons Gerard Joseph Voragen, Hendrik Arie Schols
Abstract: A modified Aspergillus oryzae cell having an area gene which does not express a functional AreA activator, DNA sequences encoding the area gene, and method for using the cell of the invention for production of a heterologous polypeptide.