Abstract: Apparatuses and methods for manipulating droplets on a printed circuit board (PCB) are disclosed. Droplets are actuated upon a printed circuit board substrate surface by the application of electrical potentials to electrodes defined on the PCB. The use of soldermask as an electrode insulator for droplet manipulation as well techniques for adapting other traditional PCB layers and materials for droplet-based microfluidics are also disclosed.

Abstract: Methods are provided for automatically crimping terminals or connectors to wires wherein the terminals or connectors are provided in strip form and may be of varying sizes, shapes, and pitches. A method according to the present invention includes providing a plurality of terminal connectors, the connectors being secured to a terminal strip in side-by-side relationship with their axes extending laterally from the strip; moving a crimping die on a ram through a working stroke towards, and a return stroke away from, a crimping anvil to crimp an individual terminal connector located therebetween onto a wire during each working stroke of the ram; and indexing the strip between successive crimping operations thereby to locate a next leading connector on the strip between the die and anvil, wherein the strip is indexed by a drive mechanism contacting the strip through pressure-engagement.

Abstract: An adaptor is shaped for allowing use of a micro SIM card in a mini SIM card implemented device. An adaptor body is shaped like a micro SIM card and may include a cutout region for receiving a micro SIM card therein.

Abstract: An apparatus and method for ground improvement includes a device having a plurality of tines extending downwardly from a top plate in a manner to achieve displacement of ground material downward and radially outward. The device is mechanically driven into the ground to achieve predetermined depths of penetration by the tines. The device is retracted and driven repeatedly to achieve densification. Optionally, voids made by the device can be filled with a flowable media.

Abstract: A droplet actuator and method of providing a droplet comprising an target substance on the droplet actuator, and including discrete flow and continuous flow functionality. Discrete flow function is controlled by electrodes arranged for conducting droplet operations on a substrate surface. The continuous flow function includes a fluid path arranged for flowing a fluid therethrough. The discrete flow and continuous flow functions may be by a barrier, including a second fluid path through the barrier. The continuous flow function may include a capture surface comprising a component having an affinity for the target substance. Methods of the invention may include flowing a fluid comprising the target substance through the fluid path; capturing the target substance on the capture surface; and forming a droplet in the discrete flow region via the second fluid path and the electrodes comprising the captured target substance.

Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a bead-containing droplet with a reduced concentration of a substance is provided, the method including providing a droplet microactuator including a droplet having a starting volume and including one or more beads and a starting concentration and starting quantity of the substance. The method further includes conducting one or more droplet operations to merge a wash droplet with the droplet provided in the above step to yield a combined droplet and including one or more droplet operations to divide the combined droplet to yield a set of droplets including a droplet including substantially all of the one or more beads and having a decreased concentration of the substance relative to the starting concentration and a droplet which is substantially lacking in the beads.

Abstract: The present invention relates to a droplet-based nucleic acid amplification apparatus and system. According to one embodiment, a droplet microactuator is provided made using a first substrate including a fluorescing material and including a detection region for detecting a fluorescence signal from a droplet, which detection region is coated with a light absorbing, low fluorescence or non-fluorescing material.

Abstract: Apparatus for inspection of a fluid comprising: a channel portion, the channel portion having a channel inlet and a channel outlet separate from the channel inlet; a piezoelectric sensor element provided at a sensor position of the channel and arranged to contact fluid flowing through the channel portion from said channel inlet to said channel outlet, the apparatus being configured to determine a difference value being a value corresponding to a difference between a resonant frequency of oscillation of said piezoelectric element at a given moment in time and a reference resonant frequency.

Abstract: A refill droplet facilitates the extraction of a droplet laterally from a channel in a microfluidic apparatus. Such extraction allows a discrete band of separated particles or solute molecules to be excised from a fluid stream and processed and analyzed separately. An extraction point is located along the length of the channel and includes an EWOD surface or similar microfluidic technology to extract a droplet. An opening in the channel opposite the extraction means is equipped with microfluidic technology to transport a refill droplet to the opening. The refill droplet is moved into the channel or column to occupy the area previously occupied by the extracted droplet. This prevents distortion or mixing of the bands of particles or molecules within the channel and prevents the draining of any portion of the fluidic system.

Abstract: An apparatus for manipulating droplets is provided. In one embodiment, the apparatus includes a substrate having a set of electrical leads for connecting electrodes to a controller, a first set of electrodes, each connected to a separate one of the electrical leads, and a second set of electrodes, all connected to a single one of the electrical leads. In another embodiment, the apparatus includes a substrate having a set of X electrodes, and a set of Y electrical leads, each connected to one or more electrodes, wherein X is greater than Y.

Abstract: Alternative approaches to fabricating printed circuit boards for use in droplet actuator operations are provided. In one embodiment, a method of manufacturing a droplet actuator for conducting droplet operations includes positioning a dielectric material between a first metal layer configured to include an electrode and a second metal layer configured to include an interconnect pad. The method additionally includes forming a connection between the first and second metal layers. Droplet actuators and methods of fabricating and supporting printed circuit boards of droplet actuators are also provided.

Abstract: Apparatuses and methods are provided for a wind diverter at least partially encircling a wind turbine support column to direct the wind from an area around a wind turbine to the rotor blades of the wind turbine. The wind diverter may also reduce turbulence and increases the laminar flow of the airstream delivered to the rotor blades. Additionally, the wind diverter increases both the volume of air and the velocity of the airstream reaching the rotor so as to increase the rotor speed. Increasing the rotor speed increases the energy available to the wind turbine. Energy increases as the cube of the rotor speed and the increased energy produces an increase in the power generated by the wind turbine. The wind diverter also allows a wind turbine to begin producing energy at lower initial wind speeds due to the concentration of air.

Abstract: The present invention relates to a nanoscale or microscale particle for encapsulation and delivery of materials or substances, including, but not limited to, cells, drugs, tissue, gels and polymers contained within the particle, with subsequent release of the therapeutic materials in situ, methods of fabricating the particle by folding a 2D precursor into the 3D particle, and the use of the particle in in-vivo or in-vitro applications The particle can be in any polyhedral shape and its surfaces can have either no perforations or nano/microscale perforations The particle is coated with a biocompatible metal, e g gold, or polymer e g parvlene, layer and the surfaces and hinges of the particle are made of any metal or polymer combinations.

Abstract: The present invention relates to a nanoscale or microscale container for encapsulation and delivery of materials or substances, including, but not limited to, cells, drugs, tissue, gels and polymers contained within the container, with subsequent release of the therapeutic materials in situ, methods of fabricating the container by folding a 2D precursor into the 3D container, and the use of the container in in-vivo or in-vitro applications. The container can be in any polyhedral shape and its surfaces can have either no perforations or nano/microscale perforations. The container is coated with a biocompatible metal, e.g. gold, or polymer, e.g. parylene, layer and the surfaces and hinges of the container are made of any metal or polymer combinations.

Abstract: Extensible shells and related methods for constructing a support pier are disclosed. A shell can define an interior for holding granular construction material and define an opening for receiving the granular construction material into the interior. The shell can be flexible such that the shell expands when granular construction material is compacted in the interior of the shell. A method may include positioning the shell in the ground and filling at least a portion of the interior of the shell with the granular construction material. The granular construction material may be compacted in the interior of the shell to form a support pier.

Abstract: A primary earth penetrating mandrel formed of a hollow shell steel plate octagonal in cross-section has an upper end and a blunt lower end joined by an upwardly and outwardly tapered wall. The mandrel is driven down in the earth to simultaneously form a vertical tapered cavity while compacting the sidewall of the cavity to provide structural integrity. The mandrel is then moved upwardly from the bottom of the cavity and aggregate is deposited in the bottom of the cavity then the mandrel is lowered so that its blunt lower end densifies the aggregate by vertical vibratory action and static force and repeating these until the pier top is near the surface of the earth at which time the upper aggregate portions are densified by the primary mandrel or a secondary mandrel having a substantially larger lower end surface than the lower end surface of the primary mandrel.

Abstract: A unique, synergistic formulation for the treatment of first-degree skin burns is provided for topical use and alleviates the full spectrum of symptoms and concerns associated with epidermal burns, including pain, blistering, redness, swelling, compromised skin integrity, risk of infection and scarring. The formulation contains natural homeopathic extracts combined with a pharmaceutically acceptable carrier suitable for topical administration. The formulation may include three or more of the following homeopathic extracts: Cantharis Vesicatoria, Echinacea Angustifolia, Calendula Officinalis and Hypericum Perforatum.

Abstract: An apparatus for manipulating droplets is provided. In one embodiment, the apparatus includes a substrate having electrodes and adjacent reference elements configured for manipulating a droplet on a surface of the substrate. Other embodiments having a top plate, a footprint, and a dielectric are also included.