Patents Assigned to 10X GENOMICS, INC.
  • Patent number: 10428326
    Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.
    Type: Grant
    Filed: February 2, 2018
    Date of Patent: October 1, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Kamila Belhocine, Rajiv Bharadwaj, Christopher Hindson, Michael Schnall-Levin, Bill Lin, Anthony Makarewicz, Pranav Patel, Katherine Pfeiffer, Andrew D. Price, Mohammad Rahimi Lenji, Tobias Daniel Wheeler, Yifeng Yin
  • Patent number: 10400280
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: September 3, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov, Paul Hardenbol
  • Patent number: 10400235
    Abstract: Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.
    Type: Grant
    Filed: December 14, 2017
    Date of Patent: September 3, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Kamila Belhocine, Geoffrey McDermott, Francesca Meschi, Xinying Zheng
  • Patent number: 10392662
    Abstract: Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences.
    Type: Grant
    Filed: April 29, 2019
    Date of Patent: August 27, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Sydney Brenner, Gi Mikawa, Robert Osborne, Andrew Slatter
  • Patent number: 10395758
    Abstract: The invention described herein solves challenges in providing a proficient, rapid and meaningful analysis of sequencing data. Methods and computer program products of the invention allow for a system to receive, analyze, and display sequencing data in real-time. The invention provides solutions to several difficulties encountered in assembling short sequencing-reads, and by doing so the invention improves the worth and significance of sequencing data.
    Type: Grant
    Filed: August 27, 2014
    Date of Patent: August 27, 2019
    Assignee: 10X GENOMICS, INC.
    Inventor: Michael Schnall-Levin
  • Publication number: 20190233878
    Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.
    Type: Application
    Filed: April 3, 2019
    Publication date: August 1, 2019
    Applicant: 10X Genomics, Inc.
    Inventors: Joshua DELANEY, Shalini GOHIL, Christopher HINDSON, Adam LOWE, Andrew D. PRICE, Joseph Francis SHUGA
  • Patent number: 10366777
    Abstract: Systems and methods for analyzing first and second strings against a ground truth string are provided. A construct representing a plurality of components is obtained, each component for a different portion of the truth string. The construct comprises a plurality of measurement string sampling pools each having an identifier and a corresponding plurality of measurement samplings corresponding to one or two of the components. Each sampling has the identifier and a portion of the first or second string. Samplings are assigned to first, second or third classes when coding a portion of the first string, second string, or both the first and second string. First and second positions are tested for events by calculating a plurality of event models using assumptions on the components having samplings encompassing the first and second positions and class assignments. These assumptions are updated using the calculated models and the models are recalculated.
    Type: Grant
    Filed: December 14, 2017
    Date of Patent: July 30, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Sofia Kyriazopoulou-Panagiotopoulou, Patrick Marks
  • Patent number: 10357771
    Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.
    Type: Grant
    Filed: May 11, 2018
    Date of Patent: July 23, 2019
    Assignee: 10X Genomics, Inc.
    Inventors: Rajiv Bharadwaj, Anthony Makarewicz, Bill Kengli Lin
  • Patent number: 10343166
    Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.
    Type: Grant
    Filed: May 16, 2017
    Date of Patent: July 9, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Rajiv Bharadwaj, Kevin Ness, Debkishore Mitra, Donald Masquelier, Anthony Makarewicz, Christopher Hindson, Benjamin Hindson, Serge Saxonov
  • Patent number: 10344329
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: July 25, 2018
    Date of Patent: July 9, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov
  • Patent number: 10347365
    Abstract: A visualization system comprising a persistent memory, storing a dataset, and a non-persistent memory implements a pattern visualizing method. The dataset contains discrete attribute values for each first entity in a plurality of first entities for each second entity in a plurality of second entities. The dataset is compressed by blocked compression and represents discrete attribute values in both compressed sparse row and column formats. The discrete attribute values are clustered to assign each second entity to a cluster in a plurality of clusters. Differences in the discrete attribute values for the first entity across the second entities of a given cluster relative to the discrete attribute value for the same first entity across the other clusters are computed thereby deriving differential values. A heat map of these differential values for each first entity for each cluster is displayed to reveal the pattern in the dataset.
    Type: Grant
    Filed: February 8, 2018
    Date of Patent: July 9, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Alexander Y. Wong, Jeffrey Mellen, Kevin Wu, Paul Ryvkin
  • Patent number: 10337061
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: September 21, 2018
    Date of Patent: July 2, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov, Paul Hardenbol, Rajiv Bharadwaj, Xinying Zheng, Phillip Belgrader
  • Patent number: 10337063
    Abstract: Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences.
    Type: Grant
    Filed: February 21, 2019
    Date of Patent: July 2, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Sydney Brenner, Gi Mikawa, Robert Osborne, Andrew Slatter
  • Patent number: 10323279
    Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.
    Type: Grant
    Filed: August 1, 2018
    Date of Patent: June 18, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Christopher Hindson, Michael Schnall-Levin, Andrew Price, Paul Hardenbol, Yuan Li
  • Patent number: 10323278
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: January 19, 2018
    Date of Patent: June 18, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Phillip Belgrader, Zachary Bent, Tarjei Sigurd Mikkelsen, Paul Ryvkin, Jessica Michele Terry
  • Publication number: 20190176152
    Abstract: A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.
    Type: Application
    Filed: August 23, 2017
    Publication date: June 13, 2019
    Applicant: 10X Genomics, Inc.
    Inventors: Rajiv Bharadwaj, Tobias Daniel Wheeler, Kevin Ness, Benjamin Hindson
  • Patent number: 10287623
    Abstract: The present invention is directed to methods, compositions and systems for capturing and analyzing sequence information contained in targeted regions of a genome. Such targeted regions may include exomes, partial exomes, introns, combinations of exonic and intronic regions, genes, panels of genes, and any other subsets of a whole genome that may be of interest.
    Type: Grant
    Filed: June 6, 2016
    Date of Patent: May 14, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Mirna Jarosz, Michael Schnall-Levin, Serge Saxonov, Benjamin Hindson, Xinying Zheng
  • Patent number: 10280459
    Abstract: Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences.
    Type: Grant
    Filed: January 29, 2019
    Date of Patent: May 7, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Sydney Brenner, Gi Mikawa, Robert Osborne, Andrew Slatter
  • Patent number: 10273541
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: August 1, 2018
    Date of Patent: April 30, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov, Paul Hardenbol
  • Patent number: 10253364
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: May 15, 2018
    Date of Patent: April 9, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mima Jarosz, Serge Saxonov, Paul Hardenbol, Rajiv Bharadwaj, Xinying Zheng, Phillip Belgrader