Patents Assigned to Affymax Technologies N.V.
  • Publication number: 20120258868
    Abstract: The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
    Type: Application
    Filed: June 14, 2011
    Publication date: October 11, 2012
    Applicants: Affymax Technologies N.V., AFFYMETRIX, INC.
    Inventors: Ronald J. Sapolsky, Robert J. Lipshutz, Thomas R. Gingeras
  • Publication number: 20050186592
    Abstract: A method and device for forming large arrays of polymers on a substrate (401). According to a preferred aspect of the invention, the substrate is contacted by a channel block (407) having channels (409) therein. Selected reagents are delivered through the channels, the substrate is rotated by a rotating stage (403), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodolgies.
    Type: Application
    Filed: November 24, 2004
    Publication date: August 25, 2005
    Applicants: Affymetrix, Inc., AFFYMAX TECHNOLOGIES N.V.
    Inventors: James Winkler, Stephen Fodor, Christopher Buchko, Debra Ross, Lois Aldwin, Douglas Modlin
  • Publication number: 20050186683
    Abstract: A method and device for forming large arrays of polymers on a substrate (401). According to a preferred aspect of the invention, the substrate is contacted by a channel block (407) having channels (409) therein. Selected reagents are delivered through the channels, the substrate is rotated by a rotating stage (403), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodolgies.
    Type: Application
    Filed: November 24, 2004
    Publication date: August 25, 2005
    Applicants: Affymetrix, Inc., AFFYMAX TECHNOLOGIES N.V.
    Inventors: James Winkler, Stephen Fodor, Christopher Buchko, Debra Ross, Lois Aldwin, Douglas Modlin
  • Publication number: 20050158744
    Abstract: Libraries of unimolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a related aspect, the present invention provides libraries of conformationally restricted probes on a solid support. The probes are restricted in their movement and flexibility using double-stranded oligonucleotides as scaffolding. The probes are also useful in various screening procedures associated with drug discovery and diagnosis. The present invention further provides methods for the preparation and screening of the above libraries.
    Type: Application
    Filed: November 19, 2004
    Publication date: July 21, 2005
    Applicants: Affymetrix, Inc., Affymax Technologies, N.V., a Netherlands Antilles corporation
    Inventors: David Lockhart, Dirk Vetter, Martin Diggelmann
  • Publication number: 20050124000
    Abstract: A method and device for forming large arrays of polymers on a substrate (401). According to a preferred aspect of the invention, the substrate is contacted by a channel block (407) having channels (409) therein. Selected reagents are delivered through the channels, the substrate is rotated by a rotating stage (403), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodolgies.
    Type: Application
    Filed: November 24, 2004
    Publication date: June 9, 2005
    Applicants: Affymetrix, Inc., AFFYMAX TECHNOLOGIES N.V., a Netherlands Antilles corporation
    Inventors: James Winkler, Stephen Fodor, Christopher Buchko, Debra Ross, Lois Aldwin, Douglas Modlin
  • Publication number: 20050026212
    Abstract: The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
    Type: Application
    Filed: September 16, 2004
    Publication date: February 3, 2005
    Applicants: Affymetrix, INC., Affymax Technologies N.V.
    Inventors: Ronald Sapolsky, Robert Lipshutz, Thomas Gingeras
  • Publication number: 20040265842
    Abstract: The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
    Type: Application
    Filed: October 29, 2003
    Publication date: December 30, 2004
    Applicants: Affymetrix, INC., Affymax Technologies N.V.
    Inventors: Ronald J. Sapolsky, Robert J. Lipshutz, Thomas R. Gingeras
  • Publication number: 20040029115
    Abstract: Means for simultaneous parallel sequence analysis of a large number of biological polymer macromolecules. Apparatus and methods may use fluorescent labels in repetitive chemistry to determine terminal manomers on solid phase immobilized polymers. Reagents which specifically recognize terminal manomers are used to label polymers at defined positions on a solid substrate.
    Type: Application
    Filed: February 14, 2002
    Publication date: February 12, 2004
    Applicant: Affymax Technologies, N.V.
    Inventors: William J. Dower, Stephen P.A. Fodor
  • Publication number: 20030082831
    Abstract: A synthetic strategy for the creation of large scale chemical diversity. Solid-phase chemistry, photolabile protecting groups, and photolithography are used to achieve light-directed spatially-addressable parallel chemical synthesis. Binary masking techniques are utilized in one embodiment. A reactor system, photoremovable protective groups, and improved data collection and handling techniques are also disclosed. A technique for screening linker molecules is also provided.
    Type: Application
    Filed: September 30, 2002
    Publication date: May 1, 2003
    Applicant: AFFYMAX TECHNOLOGIES, N.V., a Netherlands Antilles corporation
    Inventors: Stephen P.A. Fodor, Lubert Stryer, James L. Winkler, Christopher P. Holmes, Dennis W. Solas
  • Publication number: 20030082611
    Abstract: The present invention is generally directed to the evolution of new metabolic pathways and the enhancement of bioprocessing through a process herein termed recursive sequence recombination. Recursive sequence recombination entails performing iterative cycles of recombination and screening or selection to “evolve” individual genes, whole plasmids or viruses, multigene clusters, or even whole genomes. Such techniques do not require the extensive analysis and computation required by conventional methods for metabolic engineering.
    Type: Application
    Filed: September 17, 2002
    Publication date: May 1, 2003
    Applicant: Affymax Technologies N.V.
    Inventors: Jeremy Minshull, Willem P.C. Stemmer
  • Publication number: 20030008302
    Abstract: Means for simultaneous parallel sequence analysis of a large number of biological polymer macromolecules. Apparatus and methods may use fluorescent labels in repetitive chemistry to determine terminal manomers on solid phase immobilized polymers. Reagents which specifically recognize terminal manomers are used to label polymers at defined positions on a solid substrate.
    Type: Application
    Filed: February 14, 2002
    Publication date: January 9, 2003
    Applicant: Affymax Technologies, N.V.
    Inventors: William J. Dower, Stephen P.A. Fodor
  • Patent number: 6271232
    Abstract: Novel inhibitors of metalloproteases, in particular collagenase-1 and stromelysin-1, are disclosed. Such compounds are useful in pharmaceutical compositions and methods for treating or controlling disease states or conditions which involve tissue breakdown, such as rheumatoid arthritis.
    Type: Grant
    Filed: July 9, 1999
    Date of Patent: August 7, 2001
    Assignee: Affymax Technologies, N.V.
    Inventors: David Campbell, Gary C. Look, Anna Katrin Szardenings, Dinesh V. Patel
  • Patent number: 6265552
    Abstract: Biotinylation peptides can be fused to other peptides or proteins of interest using recombinant DNA techniques to provide efficient methods for biotinylating the resulting fusion proteins in vivo or in vitro.
    Type: Grant
    Filed: February 24, 2000
    Date of Patent: July 24, 2001
    Assignee: Affymax Technologies N.V.
    Inventor: Peter J. Schatz
  • Patent number: 6165717
    Abstract: A general stochastic method for synthesizing random oligomers on particles is disclosed. A further aspect of the invention relates to the use of identification tags on the particles to facilitate identification of the sequence of the monomers in the oligomer.
    Type: Grant
    Filed: May 13, 1998
    Date of Patent: December 26, 2000
    Assignee: Affymax Technologies N.V.
    Inventors: William J. Dower, Ronald W. Barrett, Mark A. Gallop
  • Patent number: 6165778
    Abstract: A device and method for efficiently synthesizing diverse molecular products on substrates. A parent vessel 200 contains a suspension of substrates. The suspension is pressurized with argon and transferred to a plurality of reaction vessels 201-209 in one or more reaction vessel banks where monomer addition reactions take place. Optionally, the substrates may be tagged with a tag monomer. A vortexing motor 300 vortexes the contents of reaction vessels 201-209 during monomer addition reactions to enhance synthesis. After the desired monomer and/or tag monomer addition reaction, the suspension is pressurized with argon and transferred back to parent vessel 200 for mixing. Thereafter, the suspension may be pressurized with argon and reallocated among reaction vessels 201-209 for further synthesis.
    Type: Grant
    Filed: July 2, 1998
    Date of Patent: December 26, 2000
    Assignee: Affymax Technologies N.V.
    Inventor: Haim Kedar
  • Patent number: 6156511
    Abstract: A random peptide library constructed by transforming host cells with a collection of recombinant vectors that encode a fusion protein comprised of a DNA binding protein and a random peptide and also encode a binding site for the DNA. binding protein can be used to screen for novel ligands. The screening method results in the formation of a complex comprising the fusion protein bound to a receptor through the random peptide ligand and to the recombinant DNA vector through the DNA binding protein.
    Type: Grant
    Filed: January 21, 1998
    Date of Patent: December 5, 2000
    Assignee: Affymax Technologies N.V.
    Inventors: Peter J. Schatz, Millard G. Cull, Jeff F. Miller, Willem Peter Christiaan Stemmer, Christian M. Gates
  • Patent number: 6143497
    Abstract: A general stochastic method for synthesizing random oligomers on particles is disclosed. A further aspect of the invention relates to the use of identification tags on the particles to facilitate identification of the sequence of the monomers in the oligomer.
    Type: Grant
    Filed: March 6, 1998
    Date of Patent: November 7, 2000
    Assignee: Affymax Technologies N.V.
    Inventors: William J. Dower, Ronald W. Barrett, Mark A. Gallop
  • Patent number: 6140493
    Abstract: A general stochastic method for synthesizing random oligomers can be used to synthesize compounds to screen for desired properties. The use of identification tags on the oligomers facilitates identification of oligomers with desired properties.
    Type: Grant
    Filed: September 11, 1998
    Date of Patent: October 31, 2000
    Assignee: Affymax Technologies N.V.
    Inventors: William J. Dower, Ronald W. Barrett, Mark A. Gallop, Michael C. Needels
  • Patent number: 6107059
    Abstract: A random peptide library constructed by transforming host cells with a collection of recombinant vectors that encode a fusion protein comprised of a carrier protein fused to a random peptide through a proteolytic cleavage site can be used to identify ligands that bind to a receptor. The screening method results in the formation of a complex comprising the fusion protein bound to a receptor through the random peptide ligand, and the random peptide can easily be identified and analyzed by virtue of the carrier protein and associated proteolytic cleavage site.
    Type: Grant
    Filed: April 29, 1992
    Date of Patent: August 22, 2000
    Assignee: Affymax Technologies N.V.
    Inventor: Charles P. Hart
  • Patent number: 6056926
    Abstract: A device and method for efficiently synthesizing diverse molecular products on substrates. A parent vessel 200 contains a suspension of substrates. The suspension is pressurized with argon and transferred to a plurality of reaction vessels 201-209 in one or more reaction vessel banks where monomer addition reactions take place. Optionally, the substrates may be tagged with a tag monomer. A vortexing motor 300 vortexes the contents of reaction vessels 201-209 during monomer addition reactions to enhance synthesis. After the desired monomer and/or tag monomer addition reaction, the suspension is pressurized with argon and transferred back to parent vessel 200 for mixing. Thereafter, the suspension may be pressurized with argon and reallocated among reaction vessels 201-209 for further synthesis.
    Type: Grant
    Filed: July 23, 1996
    Date of Patent: May 2, 2000
    Assignee: Affymax Technologies N.V.
    Inventors: Jeffrey H. Sugarman, Richard P. Rava, Haim Kedar