Abstract: Methods and systems for estimating gas supply pressure are described herein. The method can include measuring a runtime valve duty cycle for a valve of a gas chromatography system. The method can include estimating a runtime supply pressure from the runtime valve duty cycle and at least one of a runtime flow rate or downstream pressure based on one or more calibration valve duty cycles corresponding to one or more calibration supply pressures and one or more calibration flow rates. The method can include, responsive to determining the runtime supply pressure is greater than a threshold supply pressure value, generating a notification that the runtime supply pressure is greater than the threshold supply pressure value.

Abstract: A column including particles having an average particle size ranging from about 1 ?m to about 5 ?m; the particles have an average pore size ranging from about 450 ? to about 3000 ?; and the particles have an average pore volume ranging from about 0.1 cm3/g to about 5 cm3/g is disclosed. The column can be a size exclusion chromatography column. The column can be used in a method to separate monomers from viral analytes, which is also disclosed.

Abstract: A composition, including an alkaloid, and a diaryl ketone is disclosed. Also, disclosed is a kit including one or more containers in which each of the one or more containers includes the composition. A method of using a standard composition is also disclosed.

Abstract: The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in hybridization. Compositions for use in the invention include an aqueous composition comprising at least one nucleic acid sequence and at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.

Abstract: In some examples, a system may include at least two conveyors disposed at a specified distance apart and movable toward each other to reduce the specified distance or away from each other to increase the specified distance. The at least two conveyors may be operable to move a container that is contiguously disposable between the at least two conveyors along a first direction when the at least two conveyors are operated in the first direction and along a second direction that is opposite to the first direction when the at least two conveyors are operated in the second direction.

Abstract: There are disclosed methods and apparatus for automatic analysis of pharmaceutical dosage forms. In some aspects a dosage form may be grasped by a gripper which is used to bring the dosage form to a test location and to present the dosage form in a plurality of different alignments between delivery and collection optics. Probe light scattered through the dosage form is collected during each alignment. The collected probe light for the plurality of alignments is then used for Raman spectral analysis of the dosage form. In other aspects a rotation stage is used to rotate a dosage form on the stage to a preferred alignment before being grasped by a gripper. The gripper is then used to carry the dosage form to the test location for optical analysis.

Abstract: Novel tools and techniques are provided for implementing software licensing management and authentication. In various examples, a computing system might receive a request for an unlock code for a requested service from a user using a client device on which a first software is executed, the request comprising a generated unique identifier associated with the requested service and/or the first software. The generated unique identifier comprises licensing information associated with the requested service and/or the first software. In response to receiving the generated unique identifier, the computing system might determine whether the user should have access to the requested service using the first software based at least in part on the licensing information. If so, the computing system might generate an unlock code associated with the requested service using the first software, might deduct cost associated with the licensing information, and might send the unlock code to the user.

Abstract: A fluid processing apparatus includes a first fluid processing unit and a second fluid processing unit. A fluid unit is fluidically coupled to the first fluid processing unit and includes a first buffering unit configured for buffering fluid, and/or a mask flow source configured for providing a mask fluid or a flow thereof. A first coupling point is fluidically coupled between the first fluid processing unit and the fluid unit. A modulation unit is configured, selectively, for withdrawing fluid from the first coupling point, or for transferring withdrawn fluid into the second fluid processing unit. The apparatus may be utilized for chromatography.

Abstract: A vacuum pumping system includes a plurality of positive displacement vacuum pumps, and more particularly a plurality of positive displacement vacuum pumps working in parallel. The vacuum pumping system includes a management unit that carries out a synchronized control of all the positive displacement vacuum pumps of the vacuum pumping system and thus allows to check possible risk of contamination of the vacuum pumping system and carry out, if needed, the necessary corrective actions without requiring any modification to the construction of the vacuum pumping system.

Abstract: A vacuum pumping system includes a vacuum pump and a motor for driving the vacuum pump. A motor stator and a motor rotor are received in a pumping chamber of the vacuum pump. Thanks to such arrangement, the vacuum pumping can be made as a single, sealed unit and the need for dynamic seals can be avoided. Moreover, the vacuum pumping system can be more compact and lighter than prior vacuum pumping systems. A pump rotor of the vacuum pump may be at least partially made as a hollow body, and the motor may be received inside the pump rotor.

Abstract: A substrate orientation device includes a frame that supports a substrate on which liquid is dispensed, for example as may relate to (bio)chemical microarray fabrication. The frame is movable to different positions, such as for mounting the substrate to the frame, depositing droplets on the substrate, and applying a bulk liquid to the substrate. The substrate orientation device may be movable to different locations, such as different liquid dispensing devices, for example a printer and a flow cell.

Abstract: A method of handling a fluidic sample includes taking fluidic sample from a sample source by a sample shaping tool so that a pre-shaped planar fluidic sample is held by the sample shaping tool with at least one main surface, or both opposing main surfaces, of the pre-shaped planar fluidic sample being exposed, and processing the pre-shaped planar fluidic sample such as by separating the pre-shaped planar fluidic sample in a sample separation device.

Abstract: A configurable injector for injecting a fluidic sample in a separation path of a sample separation apparatus includes a sample accommodation volume for accommodating the fluidic sample to be injected into the separation path, a valve arrangement fluidically couplable with the separation path, fluidically coupled with the sample accommodation volume, and being controllable for injecting the fluidic sample into the separation path, an input interface configured for receiving input data indicative of an injection profile of an injector to be emulated by the configurable injector, and a control unit configured for controlling the configurable injector, in particular the valve arrangement, so that the configurable injector is operated in accordance with the injection profile to thereby emulate the injector to be emulated.

Abstract: Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.

Abstract: In some examples, a three-dimensional printed nanospray interface for mass spectrometry may include a body including a sheath inlet connected to a sheath outlet for passage of sheath liquid to an emitter. The emitter may include a sidewall including a stepped texture on an inner surface of the sidewall.

Abstract: A sample management device which comprises a source flow path in which a fluidic sample can flow, a volume flow adjustment unit configured for adjusting a volume flow of the fluidic sample to be branched off from the source flow path at a fluidic coupling point, and a fluidic valve fluidically coupled with the source flow path and with the volume flow adjustment unit, wherein the fluidic valve is switchable into a branch off state in which the fluidic coupling point is established within the source flow path to branch off an adjustable volume of the fluidic sample from the source flow path via the fluidic coupling point while a flow of the fluidic sample in the source flow path continues.

Abstract: The present invention is directed to a computer-implemented method of automatically identifying the presence of one or more elements in a sample via optical emission spectroscopy.

Abstract: A rocker valve for opening or closing a fluid connection includes a valve chamber into which at least one opening feeds, a pivotally mounted rocker switch, and a membrane surface located between the rocker switch and the opening. By pivoting the rocker switch, the opening can be fluidically opened or closed relative to the valve chamber. The rocker valve further includes a sealing element that is located between the membrane surface and the opening when the rocker switch closes the opening relative to the valve chamber.

Abstract: In some examples, an apparatus may include an advection flow structure including a passage to transport, via advection by a gas flow, an ablated sample from an ablation region to an ionization region.