Patents Assigned to Agilent Technology, Inc.
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Publication number: 20180262699Abstract: An apparatus and method for generating images of specimens is disclosed. The apparatus includes an imaging system, controller, and user interface. The imaging system generates a plurality of component images of a specimen, each component image corresponding to a different viewing condition. Each image is represented by an intensity as a function of location on the specimen. The controller stores the component images and generates a compound image from a plurality of the component images. The compound image includes a weighted sum of first and second ones of the component images, the controller displaying the compound image on a display controlled by the controller. The user interface is adapted to control a weighting factor used in generating the weighted sum in response to user input. The controller redisplays the compound image after the weighting factor is changed in response to user input.Type: ApplicationFiled: May 9, 2018Publication date: September 13, 2018Applicant: Agilent Technologies, Inc.Inventor: Andrew Ghetler
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Patent number: 10073012Abstract: A filter for filtering debris out of a fluid flowing along a fluid flow direction in a fluidic member of a sample separation device, the filter comprising a plurality of filter structures stacked along the fluid flow direction and each having pores with defined pore size, wherein the defined pore size of the stacked filter structures decreases along the fluid flow direction.Type: GrantFiled: September 4, 2011Date of Patent: September 11, 2018Assignee: Agilent Technologies, Inc.Inventors: Klaus Witt, Hans-Georg Haertl
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Patent number: 10073068Abstract: A liquid chromatography (LC) column includes a wall having a length along a central axis from the inlet end to the outlet end, the wall enclosing a column interior and having a column radius relative to the central axis, the wall comprising a structured portion configured such that the column radius varies along the length; and a plurality of particles packed in the column interior, wherein at least some of the particles are in contact with the structured portion.Type: GrantFiled: August 19, 2014Date of Patent: September 11, 2018Assignee: Agilent Technologies, Inc.Inventors: Hongfeng Yin, Reid A. Brennen, Eric Lyster, Roger Slocum
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Patent number: 10072261Abstract: Aspects of the present disclosure include methods for double coupling a nucleoside phosphoramidite during synthesis of an oligonucleotide. The method can include coupling a free hydroxyl group of a nucleoside residue with a first sample of a protected nucleoside phosphoramidite via an internucleoside P(III) linkage, followed by exposure to an oxidizing agent prior to a second coupling step with a second sample of the protected nucleoside phosphoramidite, and further exposure to an oxidizing agent. The method finds use in synthesizing an oligonucleotide on a solid phase support, such as a planar surface. The double coupling method can be utilized at one or more nucleotide positions during oligonucleotide synthesis thereby reducing single base deletion rates. Oligonucleotide containing compositions synthesized according to the disclosed methods are also provided.Type: GrantFiled: March 27, 2017Date of Patent: September 11, 2018Assignee: Agilent Technologies, Inc.Inventors: Joel Myerson, Siyuan Chen
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Automatic determination of demultiplexing matrix for ion mobility spectrometry and mass spectrometry
Patent number: 10037873Abstract: Multiplexed ion mobility spectrometry (IMS), mass spectrometry (MS) such as time-of-flight mass spectrometry (TOFMS), or hybrid IM-MS is carried out on a sample, and the resulting measurement data are deconvoluted. A pulse sequence controlling ion pulsing is utilized in conjunction with the multiplexing. The pulse sequence may be modified based on the raw measurement data acquired. A demultiplexing matrix based on the modified pulse sequence is utilized to improve deconvolution.Type: GrantFiled: October 2, 2015Date of Patent: July 31, 2018Assignee: Agilent Technologies, Inc.Inventors: Jun Wang, Ruwan T. Kurulugama -
Patent number: 10012574Abstract: Provided herein is a method comprising one or more of the following steps: (a) lysing cells of a biological sample and contacting the biological sample with an amount of ionic liquid sufficient to denature intracellular metabolic enzymes in the biological sample to produce a contacted cellular sample; (b) mixing the contacted cellular sample with an organic solvent to produce an ionic liquid-organic solvent composition; (c) mixing the contacted cellular sample with the organic solvent to produce a dispersed microdroplet ionic liquid-organic solvent composition; (d) contacting the ionic liquid-organic solvent composition with an ion exchange composition to produce a second ionic liquid-organic solvent composition; (d) separating the ionic liquid from the organic solvent; and (e) extracting metabolites from the ionic liquid. Kits and systems for practicing the subject methods are also provided.Type: GrantFiled: March 11, 2014Date of Patent: July 3, 2018Assignee: Agilent Technologies, Inc.Inventor: James Alexander Apffel, Jr.
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Patent number: 10012621Abstract: A sealed fluidic component for use in a fluidic flow path is made by providing a composite material comprising a first material and a second material, wherein the first material and the second material are different PAEK materials with the first material having a lower melting point than the second material. The composite material is heated to provide a sealing by the first material.Type: GrantFiled: May 2, 2011Date of Patent: July 3, 2018Assignee: Agilent Technologies, Inc.Inventor: Bernhard Dehmer
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Patent number: 10012624Abstract: An apparatus for deriving an operation mode from a first fluidic device to a second fluidic device, wherein the first fluidic device has a first target operation mode representing a desired behavior of the first fluidic device and has a first real operation mode representing the actual behavior of the first fluidic device, wherein the second fluidic device has a second target operation mode representing a desired behavior of the second fluidic device and has a second real operation mode representing the actual behavior of the second fluidic device, the apparatus comprising a first determining unit configured for determining the first real operation mode based on the first target operation mode and based on a preknown parameterization of the first fluidic device, and a second determining unit configured for determining the second target operation mode based on the determined first real operation mode and based on a preknown parameterization of the second fluidic device.Type: GrantFiled: July 20, 2016Date of Patent: July 3, 2018Assignee: Agilent Technologies, Inc.Inventors: Klaus Witt, Herbert Anderer, Alwin Ritzmann, Dominik Ruf
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Patent number: 10010884Abstract: A droplet-based microfluidic device having a first confining plate, a second confining plate, and an actuator. Each confining plate includes a respective substrate and hydrophobic layer having a planar major surface. The first confining plate additionally includes a common electrode between its hydrophobic layer and substrate. The second confining plate includes an electrode array between its hydrophobic layer and substrate. The confining plates are disposed opposite one another with their major surfaces separated from one another by a gap. The actuator is to impart oscillatory sliding motion between the confining plates in a direction principally parallel to the major surfaces. The oscillatory sliding motion effectively allows voltages applied between the common electrode and the electrodes of the electrode array to move a microfluidic droplet located in the gap across the major surfaces without sticking.Type: GrantFiled: January 14, 2014Date of Patent: July 3, 2018Assignee: Agilent Technologies, Inc.Inventors: Curt A. Flory, Arthur Schleifer, Gershon Perelman
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Patent number: 10003754Abstract: An apparatus and method for generating images of specimens is disclosed. The apparatus includes an imaging system, controller, and user interface. The imaging system generates a plurality of component images of a specimen, each component image corresponding to a different viewing condition. Each image is represented by an intensity as a function of location on the specimen. The controller stores the component images and generates a compound image from a plurality of the component images. The compound image includes a weighted sum of first and second ones of the component images, the controller displaying the compound image on a display controlled by the controller. The user interface is adapted to control a weighting factor used in generating the weighted sum in response to user input. The controller redisplays the compound image after the weighting factor is changed in response to user input.Type: GrantFiled: June 18, 2015Date of Patent: June 19, 2018Assignee: Agilent Technologies, Inc.Inventor: Andrew Ghetler
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Patent number: 9995421Abstract: In a vibration damper of a turbomolecular vacuum pump, a vacuum tightness function and a vibration damping function are both performed by one ore more toroidal rings made of an elastic material. The elastic rings assume a resting configuration while the vacuum pump is not operating and the damper is at atmospheric pressure, and they assume a compressed configuration while the vacuum pump is operating and the damper is under vacuum conditions. In the compressed configuration, the elastic rings are deformed due to a compression force. This deformation of the elastic rings prevents metal-to-metal contact between the vacuum pump and a vacuum chamber to which the pump is connected through the damper.Type: GrantFiled: March 27, 2014Date of Patent: June 12, 2018Assignee: Agilent Technologies, Inc.Inventors: Carmelo Quartarone, Luca Bonmassar, Silvio Giors, Vincenzo Pandolfo
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Publication number: 20180144464Abstract: A method of operating a data processing system to automatically process a color digital image of a specimen that has been stained with a first dye and a second different dye is disclosed. The method includes receiving a color image that includes a plurality of pixels, each pixel being characterized by a pixel vector having components determined by the intensity of light in each of a corresponding number of wavelength bands. A plurality of pixel vectors of the image are transformed to a hue space divided into a plurality of bins, each bin being characterized by a number of pixels that have been transformed into that bin. The data processing system automatically finds first and second color vectors characterizing the first and second dyes, respectively based on the number of pixels that were transformed into each of the bins.Type: ApplicationFiled: November 22, 2016Publication date: May 24, 2018Applicant: Agilent Technologies, Inc.Inventors: Amir Ben-Dor, Elad Arbel
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Patent number: 9976162Abstract: Provided herein is a method comprising: producing a first complex by annealing: a first nucleic acid comprising, in order, a first unique sequence, a first central sequence and a second unique sequence; and a second nucleic acid comprising, in order, said second unique sequence, a second central sequence and a third unique sequence; wherein the first, second and third unique sequences do not hybridize with each other; subjecting said first complex to multiple rounds of primer extension to extend the first and second nucleic acids using each other as a template, thereby producing a first product molecule that contains, in order, the first unique sequence, the first central sequence, the second unique sequence, the second central sequence and the third unique sequence; and circularizing said first product molecule by intramolecularly ligating the ends of said product molecule together. Kits and compositions relating to the method are also provided.Type: GrantFiled: November 5, 2013Date of Patent: May 22, 2018Assignee: Agilent Technologies, Inc.Inventors: Jeffrey Robert Sampson, Derek Lee Lindstrom
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Patent number: 9972480Abstract: An ion guide generates a radio frequency (RF) field to radially confine ions to an ion beam along a guide axis as the ions are transmitted through the ion guide. The effective potential of the RF field includes an alternating series of barriers and wells. Ions may be trapped in individual wells in mass-dependent order, with larger masses trapped closer to a guide exit than smaller masses. The RF field may be scanned so as to release the ions from the ion guide in mass-dependent order, with larger masses released before smaller masses. The operating conditions may be set such that ions over the entire mass range arrive at a desired downstream focal point simultaneously, for example at an accelerator of a time-of-flight analyzer.Type: GrantFiled: November 25, 2015Date of Patent: May 15, 2018Assignee: Agilent Technologies, Inc.Inventor: Trygve Ristroph
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Patent number: 9962695Abstract: A method of manufacturing a sealing fluidic component based on a capillary enclosing a fluid conduit and having an exterior surface being at least partially coated with a coating of a meltable material, wherein the method comprises melting the meltable material of the coating at least at an end portion of the capillary, and resolidifying the melted material to thereby form, at the end portion, a sealing integral with the coating and constituted at least partially by the meltable material.Type: GrantFiled: August 30, 2013Date of Patent: May 8, 2018Assignee: Agilent Technologies, Inc.Inventor: Thomas Reinhardt
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Publication number: 20180119140Abstract: Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.Type: ApplicationFiled: October 3, 2017Publication date: May 3, 2018Applicants: The Board of Trustees of the Leland Stanford Junior University, Agilent Technologies, Inc.Inventors: Matthew H. Porteus, Ayal Hendel, Joe Clark, Rasmus O. Bak, Daniel E. Ryan, Douglas J. Dellinger, Robert Kaiser, Joel Myerson
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Patent number: 9958363Abstract: A method for removing an ionic liquid from an aqueous sample is provided. In some embodiments, the method includes: (a) combining an aqueous sample including an ionic liquid with an ion exchanger composition including an ion exchanger counterion to produce a solution including a fluorous salt of the ionic liquid, where at least one of the ionic liquid and the ion exchanger counterion is fluorinated; (b) contacting the solution with a fluorous affinity material, thereby removing fluorous salt from the solution and producing an aqueous eluate; and (c) collecting the aqueous eluate. In certain embodiments, the method further includes: contacting a cell with an ionic liquid composition to lyse the cell and produce an aqueous sample; and contacting the aqueous sample with a reverse phase substrate, thereby adsorbing proteins and/or lipids of the cell on the substrate. Compositions, kits and systems for practicing the subject methods are also provided.Type: GrantFiled: May 28, 2015Date of Patent: May 1, 2018Assignee: Agilent Technologies, Inc.Inventors: Brian Phillip Smart, Brooks Bond-Watts, James Alexander Apffel, Jr.
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Patent number: 9945762Abstract: A method and a system for introducing a sample into a mobile phase of a chromatography system is provided. The method includes initially directing the mobile phase directly into a separation unit of the chromatography system, bypassing a sample loop, the mobile phase including a combined solvent, metered from a pressurized first solvent and a second solvent; loading the sample into the sample loop, while the mobile phase continues to be directed directly into the separation unit; pressurizing the sample in the sample loop with the pressurized first solvent, while the mobile phase continues to be directed directly into the separation unit; and switching the sample loop into the mobile phase, thereby introducing the pressurized sample to the separation unit.Type: GrantFiled: December 30, 2014Date of Patent: April 17, 2018Assignee: Agilent Technologies, Inc.Inventors: Edwin E. Wikfors, Brian A. Bidlingmeyer, Klaus Witt
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Patent number: 9945820Abstract: In a liquid chromatography system, a sample is injected into a column by flowing a solvent mixture from a mixer into the column along a solvent mixture flow path; and injecting a sample into the solvent mixture flow path downstream of the mixer. In another liquid chromatography system, a sample is injected into a column by loading an isolator fluid into a sample loop, loading a sample into the sample loop, and flowing the sample into the column as a plug in front of the isolator fluid.Type: GrantFiled: October 28, 2013Date of Patent: April 17, 2018Assignee: Agilent Technologies, Inc.Inventor: Robert William Albrecht, Jr.
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Patent number: D822848Type: GrantFiled: February 28, 2017Date of Patent: July 10, 2018Assignee: Agilent Technologies, Inc.Inventors: Gilbert Lemke, Thomas E. Price, Jonathan Michael Frentzel, Rafael Mulero