Patents Assigned to Akonni Biosystems, Inc.
  • Patent number: 10532352
    Abstract: An integrated sample analysis system is disclosed. The sample analysis system contains (1) a sample preparation/analysis module having sample purification device comprising a monolith that binds specifically to nucleic acids and a sample analysis device comprising a microarray enclosed in a reaction chamber having a hydrophilic interior surface; (2) a temperature control module comprising a thermocycler having a thermally conductive temperature-control bladder; and (3) an imaging device capable of capturing an image of the microarray in the reaction chamber.
    Type: Grant
    Filed: April 3, 2019
    Date of Patent: January 14, 2020
    Assignee: Akonni Biosystems, Inc.
    Inventors: Christopher G. Cooney, Phillip Belgrader, Arial Bueno, Steve Garber, Nitu Harshendu Thakore, Peter Qiang Qu
  • Patent number: 10300482
    Abstract: An integrated sample analysis system is disclosed. The sample analysis system contains (1) a sample preparation/analysis module having sample purification device comprising a monolith that binds specifically to nucleic acids and a sample analysis device comprising a microarray enclosed in a reaction chamber having a hydrophilic interior surface; (2) a temperature control module comprising a thermocycler having a thermally conductive temperature-control bladder; and (3) an imaging device capable of capturing an image of the microarray in the reaction chamber.
    Type: Grant
    Filed: December 8, 2011
    Date of Patent: May 28, 2019
    Assignee: AKONNI BIOSYSTEMS, INC.
    Inventors: Christopher G. Cooney, Phillip Belgrader, Arial Bueno, Steve Garber, Nitu Harshendu Thakore, Peter Qiang Qu
  • Patent number: 10138458
    Abstract: A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.
    Type: Grant
    Filed: December 22, 2015
    Date of Patent: November 27, 2018
    Assignee: Akonni Biosystems, Inc.
    Inventors: Phillip Belgrader, Benjamin Hindson
  • Patent number: 10125388
    Abstract: An integrated sample purification system includes a housing, a sample container rack, a filter holder, and a cylindrical magnet. The sample container rack and the filter device holder are disposed in the housing. The sample container rack is configured to hold one or more sample containers, the filter device holder is configured to hold one or more filter devices. The cylindrical magnet is adjacent to and external to the sample container rack, and is rotated about a central, longitudinal axis of the magnet by an electric motor disposed in the housing to lyse cells. Molecules of interest in the lysed cells are purified using filters that bind specifically to the molecules of interest. The system is readily amenable to automation and rapid purification and analysis of molecules of interest, such as nucleic acids and proteins.
    Type: Grant
    Filed: December 1, 2015
    Date of Patent: November 13, 2018
    Assignee: AKONNI BIOSYSTEMS, INC.
    Inventors: Christopher G. Cooney, Rebecca Holmberg, Phillip Belgrader, Peter Qiang Qu
  • Patent number: 10093919
    Abstract: Methods for automated extraction of nucleic acids are disclosed. Also disclosed are method and kits for isolating fetal nucleic acids from a plasma sample of a pregnant woman. The method includes flowing the plasma sample through a first filter under conditions that allow binding of the fetal and maternal nucleic acids to the first filter; eluting the fetal and maternal nucleic acids bound to the first filter to produce a concentrated nucleic acid sample; flowing the concentrated nucleic acid sample through a second filter under conditions that allow preferential binding of the maternal nucleic acids to the second filter; and recovering the fetal nucleic acid from the concentrated nucleic acid sample that flow through the second filter.
    Type: Grant
    Filed: July 20, 2016
    Date of Patent: October 9, 2018
    Assignee: Akonni Biosystems, Inc.
    Inventors: Rebecca Holmberg, Alissa Erin Burkey, Tinsley Janna Stokes, Phillip Belgrader
  • Patent number: 10081016
    Abstract: A molecular testing device comprises a heating and cooling module having a thin-film thermoelectric heating and cooling device, and a removable test module having a combined amplification and hybridization reaction chamber. The reaction chamber comprises a thermo-conductive exterior surface and a microarray on an interior surface. The thin-film thermoelectric heating and cooling device has a heat transfer surface that is adapted to make contact with the thermo-conductive exterior surface of the reaction chamber. The molecular testing device may be used to perform a PCR in the reaction chamber.
    Type: Grant
    Filed: June 18, 2015
    Date of Patent: September 25, 2018
    Assignee: Akonni Biosystems, Inc.
    Inventors: Christopher G. Cooney, Peter Qiang Qu, Arial Bueno
  • Patent number: 9753047
    Abstract: A positioning system for a sample analysis device is disclosed. The positioning system comprises (1) a carousel comprising a platform and a sample loading tray mounted on the platform, and (2) a stage comprising a positioning system for positioning the carousel under the optical path of an imaging system. The sample loading tray is configured for holding a cartridge comprising one or more lateral flow cells (LFCs).
    Type: Grant
    Filed: October 26, 2015
    Date of Patent: September 5, 2017
    Assignee: AKONNI BIOSYSTEMS INC.
    Inventors: Christopher Cooney, Alexander Perov, Arial Bueno, Charles Daitch
  • Patent number: 9493815
    Abstract: A microarray assembly for detection of a target molecule is disclosed. The microarray assemblies comprise an array chamber having a microarray located therein and features that facilitate liquid movement within the array chamber. Also disclosed are methods for making the microarray assembly using rollable films and methods for detecting microarray spots using an internal control fluorophore in the array spot.
    Type: Grant
    Filed: June 3, 2014
    Date of Patent: November 15, 2016
    Assignee: AKONNI BIOSYSTEMS, INC.
    Inventors: Christopher G. Cooney, Peter Qiang Qu, Alexander Perov, Jennifer Parker
  • Patent number: 9428746
    Abstract: Methods for automated extraction of nucleic acids are disclosed. Also disclosed are method and kits for isolating fetal nucleic acids from a plasma sample of a pregnant woman. The method includes flowing the plasma sample through a first filter under conditions that allow binding of the fetal and maternal nucleic acids to the first filter; eluting the fetal and maternal nucleic acids bound to the first filter to produce a concentrated nucleic acid sample; flowing the concentrated nucleic acid sample through a second filter under conditions that allow preferential binding of the maternal nucleic acids to the second filter; and recovering the fetal nucleic acid from the concentrated nucleic acid sample that flow through the second filter.
    Type: Grant
    Filed: August 27, 2013
    Date of Patent: August 30, 2016
    Assignee: Akonni Biosystems, Inc.
    Inventors: Rebecca Holmberg, Alissa Erin Gindlesperger, Tinsley Janna Stokes, Phillip Belgrader
  • Patent number: 9217174
    Abstract: A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: December 22, 2015
    Assignees: Akonni Biosystems, Inc., Bio-Rad Laboratories
    Inventors: Phillip Belgrader, Benjamin Hindson
  • Patent number: 9074245
    Abstract: A microarray-based sample analysis (MBSA) system includes a cartridge holder adapted to receive a replaceable cartridge that is configured to receive a detachable, replaceable sample analysis unit containing one or more reaction chambers for sample analysis; a fluid control subsystem that controls fluid flow; and an optical subsystem configured to capture an image of the microarray.
    Type: Grant
    Filed: July 5, 2012
    Date of Patent: July 7, 2015
    Assignee: AKONNI BIOSYSTEMS, INC
    Inventors: Phillip Belgrader, Christopher G. Cooney, Aleksandr N. Perov
  • Patent number: 8906652
    Abstract: Methods and devices for performing chemical reactions under controlled temperature are described. In one embodiment, the devices provided by the invention comprise a housing dimensioned to hold a reaction chamber disposed within an interior volume of the housing. The reaction chamber has thermally conductive interior and exterior surfaces defining an internal volume therein at a first temperature. The device also includes at least one temperature-control substance at a second temperature received into said bladder and expels said temperature-control substance, the bladder expands to about substantially at least a portion of said exterior surfaces of said reaction chamber to enable thermal exchange between said temperature-control substance and the said internal volume of reaction chamber.
    Type: Grant
    Filed: August 1, 2012
    Date of Patent: December 9, 2014
    Assignee: Akonni Biosystems, Inc.
    Inventor: Phillip Belgrader
  • Publication number: 20140287954
    Abstract: A microarray assembly for detection of a target molecule is disclosed. The microarray assemblies comprise an array chamber having a microarray located therein and features that facilitate liquid movement within the array chamber. Also disclosed are methods for making the microarray assembly using rollable films and methods for detecting microarray spots using an internal control fluorophore in the array spot.
    Type: Application
    Filed: June 3, 2014
    Publication date: September 25, 2014
    Applicant: AKONNI BIOSYSTEMS, INC.
    Inventors: Christopher G. COONEY, Peter Qiang QU, Alexander PEROV, Jennifer PARKER
  • Patent number: 8828912
    Abstract: A microarray assembly for detection of a target molecule is disclosed. The microarray assemblies comprise an array chamber having a microarray located therein and features that facilitate liquid movement within the array chamber. Also disclosed are methods for making the microarray assembly using rollable films and methods for detecting microarray spots using an internal control fluorophore in the array spot.
    Type: Grant
    Filed: April 13, 2012
    Date of Patent: September 9, 2014
    Assignee: Akonni Biosystems, Inc.
    Inventor: Christopher G. Cooney
  • Patent number: 8759085
    Abstract: A device for controlling temperature in a reaction chamber is disclosed. The device comprises: a bladder assembly comprising a housing dimensioned to hold a reaction chamber disposed within an interior volume of the housing; and a first temperature-control bladder disposed within the housing, the first temperature-control bladder is configured to receive a temperature-control fluid and comprises a flexible, heat conductive surface that comes in contact with at least a portion of an exterior surface of the reaction chamber after receiving the temperature-control fluid. Also disclosed are a bladder thermal cycler, a temperature-control bladder assembly and methods for producing a thermal cycle in a reaction chamber.
    Type: Grant
    Filed: April 29, 2011
    Date of Patent: June 24, 2014
    Assignee: Akonni Biosystems, Inc.
    Inventors: Phil Belgrader, Christopher G. Cooney, Robert Doebler, Anna Hickerson, Bruce Irvine, Ali Nadim, James Sterling, Reza Miraghaie
  • Patent number: 8680025
    Abstract: A microarray system is disclosed. The microarray system includes a microarray formed on a planar substrate and an incubation chamber formed around the microarray. The incubation chamber has a plurality of interior surfaces including a bottom surface on which the microarray is formed and a top surface that faces the bottom surface and is generally parallel to the bottom surface. At least one of a plurality of interior surfaces is a hydrophilic surface.
    Type: Grant
    Filed: May 9, 2008
    Date of Patent: March 25, 2014
    Assignee: Akonni Biosystems, Inc.
    Inventor: Christopher G. Cooney
  • Patent number: 8680026
    Abstract: A microarray system is disclosed. The microarray system includes a microarray formed on a planar substrate and an incubation chamber formed around the microarray. The incubation chamber has a plurality of interior surfaces including a bottom surface on which the microarray is formed and a top surface that faces the bottom surface and is generally parallel to the bottom surface. At least one of a plurality of interior surfaces is a hydrophilic surface.
    Type: Grant
    Filed: July 21, 2010
    Date of Patent: March 25, 2014
    Assignee: Akonni Biosystems, Inc.
    Inventors: Christopher G. Cooney, David E. Sipes, Rebecca C. Holmberg, Phillip Belgrader
  • Patent number: 8623789
    Abstract: An integrated cartridge for sample processing and analysis is disclosed. The integrated cartridge contains a sample preparation chamber having a sample inlet and a sample outlet, and a sample purification chamber adapted to receive a replaceable sample purification unit containing a housing and an extraction filter inside the housing. The extraction filter specifically binds to a molecule of interest. The sample purification chamber has a sample inlet that is in fluid communication with the sample outlet of the sample preparation chamber. Also disclosed is a microarray-based sample analysis (MBSA) system.
    Type: Grant
    Filed: September 20, 2010
    Date of Patent: January 7, 2014
    Assignee: Akonni Biosystems, Inc.
    Inventors: Phillip Belgrader, Christopher G. Cooney, Aleksandr N. Perov
  • Patent number: 8603783
    Abstract: A device for controlling temperature in a reaction chamber is disclosed. The device comprises: a bladder assembly comprising a housing dimensioned to hold a reaction chamber disposed within an interior volume of the housing; and a first temperature-control bladder disposed within the housing, the first temperature-control bladder is configured to receive a temperature-control fluid and comprises a flexible, heat conductive surface that comes in contact with at least a portion of an exterior surface of the reaction chamber after receiving the temperature-control fluid. Also disclosed are a bladder thermal cycler, a temperature-control bladder assembly and methods for producing a thermal cycle in a reaction chamber.
    Type: Grant
    Filed: August 1, 2012
    Date of Patent: December 10, 2013
    Assignee: Akonni Biosystems, Inc.
    Inventors: Phillip Belgrader, Christopher G. Cooney, Robert Doebler, Anna Hickerson, Bruce Irvine, Ali Nadim, James Sterling, Reza Miraghaie
  • Patent number: 8574923
    Abstract: A sample preparation device is disclosed. The sample preparation device includes a housing defining a passage way between a first opening and a second opening; and a sample filter occupying a section of said passage way. The sample filter contains a monolith adsorbent that specifically binds to nucleic acids. Also disclosed are sample filters containing glass frit is coated with an capture agent that binds specifically to an analyte of interest, sample filters containing a hydrophilic matrix with impregnated chemicals that lyses cell membranes, a cartridge base and an integrated sample preparation cartridge.
    Type: Grant
    Filed: November 20, 2012
    Date of Patent: November 5, 2013
    Assignee: Akonni Biosystems, Inc.
    Inventors: Christopher G. Cooney, Phillip Belgrader