Abstract: There is disclosed a system and methods for debriding soft tissue from bone using a high-pressure water debridement system. One embodiment includes a cylindrical sleeve bounded by endcaps and having a drainage port positioned for effluent drainage. A central shaft is configured to receive a bone segment and is disposed along a longitudinal center of the sleeve and rotatively coupled between the endcaps. At least one high-pressure water nozzle is disposed on each side of the sleeve, each of which is positioned to impact the bone segment with a high-pressure water stream. A rotational actuator is configured to rotate the central shaft and the bone segment relative to the sleeve and the water nozzles such that when the high-pressure water nozzles are operational, the high-pressure water streams debride the bone segment. Other embodiments are also disclosed.

Abstract: Embodiments of the present technology include a method of making a bone composite graft for administration to a patient. The method may include combining a human cadaveric bone material with a plurality of polymethyl methacrylate binder particles and spincasting the combined human cadaveric bone material and polymethyl methacrylate binder particles to produce the bone composite graft. The method may also include ablating the bone composite graft to increase the surface area of bone material exposed. The human cadaveric bone material may be immobilized in the plurality of polymethyl methacrylate binder particles. The human cadaveric bone material may be present in an amount that is 50 weight percent of the bone composite graft, or less. Additionally, the bone composite graft may have a yield strength that is at least 13,000 N/cm2 and no greater than 15,000 N/cm2.

Abstract: The present invention provides compositions for treating soft tissue injuries comprising a collagen matrix and mesenchymal stem cells adhered to the collagen matrix. Methods of making and using compositions comprising a collagen matrix and mesenchymal stem cells adhered to the collagen matrix are also provided.

Abstract: Compositions comprising a cartilage sheet comprising a plurality of interconnected cartilage tiles and a biocompatible carrier are provided. Methods of manufacturing cartilage compositions comprising a cartilage sheet comprising a plurality of interconnected cartilage tiles are also provided.

Abstract: There is disclosed a system and method for dispersing sterile water from a circulating high-purity water system to a processing field containing allograft tissue. One embodiment includes a fluid inlet that is fluidly coupled with and configured to receive sterile water from the water system. The dispersion system also includes at least first and second fluid outlets that are selectively operable to deliver respective first and second fluid streams into different areas of the processing field. At least one of the first and second fluid outlets may be associated with a regulator valve configured to provide an adjustable flowrate to conserve water pulled from the circulating water system to meet the needs of the application taking place within the processing field. Other embodiments are also disclosed.

Abstract: There is disclosed a system and method for dispersing sterile water from a circulating high-purity water system to a processing field containing allograft tissue. One embodiment includes a fluid inlet that is fluidly coupled with and configured to receive sterile water from the water system. The dispersion system also includes at least first and second fluid outlets that are selectively operable to deliver respective first and second fluid streams into different areas of the processing field. At least one of the first and second fluid outlets may be associated with a regulator valve configured to provide an adjustable flowrate to conserve water pulled from the circulating water system to meet the needs of the application taking place within the processing field. Other embodiments are also disclosed.

Abstract: There is disclosed a system and method for dispersing sterile water from a circulating high-purity water system to a processing field containing allograft tissue. One embodiment includes a fluid inlet that is fluidly coupled with and configured to receive sterile water from the water system. The dispersion system also includes at least first and second fluid outlets that are selectively operable to deliver respective first and second fluid streams into different areas of the processing field. At least one of the first and second fluid outlets may be associated with a regulator valve configured to provide an adjustable flowrate to conserve water pulled from the circulating water system to meet the needs of the application taking place within the processing field. Other embodiments are also disclosed.

Abstract: Compositions comprising a cartilage sheet comprising a plurality of interconnected cartilage tiles and a biocompatible carrier are provided. Methods of manufacturing cartilage compositions comprising a cartilage sheet comprising a plurality of interconnected cartilage tiles are also provided.

Abstract: Provided are systems and methods for cryopreserving tissue, particularly cartilage tissue and osteochondral tissue. Also provided are tissue products made using such systems and methods. Certain methods involve combining tissue with a cryopreservation solution in a processing vessel and applying resonant acoustic energy thereto prior to freezing the tissue. The resonant acoustic energy rapidly agitates the tissue with the cryopreservation solution by vibration. By applying resonant acoustic energy to the tissue during processing, the rate or efficiency of processing, or both, may be improved. Certain methods involve soaking tissue in a cryopreservation solution prior to freezing the tissue.

Abstract: Composite grafts including a biocompatible, synthetic scaffold; and a biological tissue component obtained or derived from a deceased donor tissue, wherein the biological tissue component is embedded in the biocompatible, synthetic scaffold, are provided as systems relating thereto. Methods of manufacture and methods of treatment using such grafts are also provided.

Abstract: There is disclosed a customizable system and methods of use for manufacturing and testing a variety of pre-sutured allograft tendon constructs sutured according to a variety of stitching patterns and featuring a variety of tissue lengths and types. One embodiment includes a triple-channel base having first, second, and third longitudinal channels extending between first and second ends of the base, as well as a plurality of tendon-manipulation accessories. Each of the tendon-manipulation accessories includes a locking-base assembly for selectively securing the accessory to the base such that the multiple tendon-manipulation accessories may be secured to the triple-channel base in a variety of custom arrangements suitable for preparing the variety of pre-sutured constructs and/or for pre-tensioning or testing the variety of the pre-sutured constructs. Other embodiments are also disclosed.

Abstract: Provided in this disclosure are various composite grafts having a trabecular scaffold with voids defined in at least a portion of the scaffold and a biological component positioned in at least some of the voids of the scaffold. The grafts may have a synthetic scaffold or a bone substrate scaffold. The grafts may be osteogenic, chondrogenic, osteochondrogenic, or vulnerary in nature. Also provided are methods of using the composite grafts to treat a tissue defect in a subject. Methods of manufacturing are also provided. Synthetic scaffolds are manufactured by additive manufacturing. Agitation is used to combine the biological component with the scaffold of the graft.

Abstract: Provided in this disclosure are various composite grafts having a trabecular synthetic scaffold with voids defined in at least a portion of the scaffold and a biological component positioned in at least some of the voids of the scaffold. The grafts may be osteogenic, chondrogenic, osteochondrogenic, or vulnerary in nature. Also provided are methods of using the composite grafts to treat a tissue defect in a subject. Methods of manufacturing are also provided. Grafts are manufactured by additive manufacturing. Agitation may be used to combine composite grafts with additional biological component.

Abstract: There is disclosed a system and method for laser cutting of human allograft tissue. One embodiment includes a laser canister having a housing fabricated with a sterilizable material. The housing defines an interior portion, an exterior portion, and a selectively operable opening into the interior portion. The selectively operable opening includes a hermetic seal to maintain a sterile environment from the exterior portion when the selectively operable opening is closed. The canister includes an infrared (IR) transmissive optical window disposed in the housing and configured to allow a laser beam to penetrate therethrough to the interior portion. The canister also includes an insert within the interior portion configured to support human allograft tissue in a sterile environment, the insert being removable from the housing to move the human allograft tissue into another sterile environment after the housing has been exposed to a non-sterile environment. Other embodiments are also disclosed.

Abstract: The disclosure provides apparatus and methods of use pertaining to a measurement template for use in allograft preparation and processing. Specifically, the template may be used to measure the trochlea angle of the trochlea groove of the distal human femur. The measurement template may be formed of a flat metal plate having first and second ends, each forming a different angle designed to fit against the trochlea groove and provide an indication of the angle of the groove in relation to the angles formed by the template. The trochlea angle of the allograft may then be documented, allowing for proper matching between allograft tissue and transplant recipient physiology. The measurement template may be adapted for a variety of configurations depending on desired and/or common allograft requirements. Other embodiments are also disclosed.

Abstract: There is disclosed a system and method for laser cutting of human allograft tissue. One embodiment includes a laser canister having a housing fabricated with a sterilizable material. The housing defines an interior portion, an exterior portion, and a selectively operable opening into the interior portion. The selectively operable opening includes a hermetic seal to maintain a sterile environment from the exterior portion when the selectively operable opening is closed. The canister includes an infrared (IR) transmissive optical window disposed in the housing and configured to allow a laser beam to penetrate therethrough to the interior portion. The canister also includes an insert within the interior portion configured to support human allograft tissue in a sterile environment, the insert being removable from the housing to move the human allograft tissue into another sterile environment after the housing has been exposed to a non-sterile environment. Other embodiments are also disclosed.

Abstract: There is disclosed a system and method for measuring the diameter or thickness of a tendon. In an embodiment, the device includes an adjustable tube having a variably sized cross-sectional diameter in a direction perpendicular to an axis extending between the first end and the second end, the adjustable tube being positionable from an open configuration in which the variably sized cross-sectional diameter is larger than a diameter of a tendon so as to allow loading of the tendon, to a closed configuration in which the variably sized cross-sectional diameter approximates the diameter of the tendon so as to allow measurement of the tendon. The device includes a measurement gradient in operable configuration with the adjustable tube so as to allow measurement of the tendon in the closed configuration. Other embodiments are also disclosed.

Abstract: There is disclosed a method of combining mesenchymal stem cells (MSCs) with a bone substrate. In an embodiment, the method includes obtaining tissue having MSCs together with unwanted cells. The tissue is digested to form a cell suspension having MSCs and unwanted cells. The cell suspension is added to the substrate. The substrate is cultured to allow the MSCs to adhere. The substrate is rinsed to remove unwanted cells. In various embodiments, the tissue is adipose tissue, muscle tissue, or bone marrow tissue. In an embodiment, there is disclosed an allograft product including a combination of MSCs with a bone substrate in which the combination is manufactured by culturing MSCs disposed on the substrate for a period of time to allow the MSCs to adhere to the substrate, and then rinsing the substrate to remove unwanted cells from the substrate. Other embodiments are also disclosed.

Abstract: There is disclosed a method of combining mesenchymal stem cells (MSCs) with a bone substrate. In an embodiment, the method includes obtaining tissue having MSCs together with unwanted cells. The tissue is digested to form a cell suspension having MSCs and unwanted cells. The cell suspension is added to the substrate. The substrate is cultured to allow the MSCs to adhere. The substrate is rinsed to remove unwanted cells. In various embodiments, the tissue is adipose tissue, muscle tissue, or bone marrow tissue. In an embodiment, there is disclosed an allograft product including a combination of MSCs with a bone substrate in which the combination is manufactured by culturing MSCs disposed on the substrate for a period of time to allow the MSCs to adhere to the substrate, and then rinsing the substrate to remove unwanted cells from the substrate. Other embodiments are also disclosed.