Abstract: There are disclosed systems and methods of debriding tissue. In an embodiment, a system includes a bone tube having an opening sized to receive a bone with tissue disposed thereon, the opening of the bone tube sized to exclude entry of an operator's hand; a spray nozzle configured to emit a water jet into the bone tube; and an outlet from the bone tube. In another embodiment, a method includes assembling a sterile debridement system; placing a sterile mesh bag over a bone tube having an opening sized to receive a bone with tissue disposed thereon, the opening of the bone tube sized to exclude entry of an operator's hand; inserting one end of a bone into the bone tube; actuating a spray nozzle directed into the bone tube, and the water jet configured to debride tissue from the bone within the bone tube. Other embodiments are also disclosed.

Abstract: Compositions comprising a plurality of cartilage particles from a human adult cadaveric donor, wherein the cartilage particles comprise viable chondrocytes, and a biocompatible carrier are provided. Methods of manufacturing cartilage compositions comprising a plurality of cartilage particles from a human adult cadaveric donor are also provided.

Abstract: There is disclosed a method of procuring allograft tissue. In an embodiment, the method includes inserting an allograft through an opening into a procurement extraction container, sealing the opening, adding extraction fluid through a second opening, agitating the fluid, collecting the fluid for detection of contamination, and sealing the second opening. There is disclosed a procurement extraction container. In one embodiment, the container includes a wall defining an interior to receive the allograft, an opening into the interior, sized to receive the allograft, a closure device for the opening, the closure device providing a hermetic seal, a second opening providing a second passageway into the interior, the second opening having a connector interface and a second closure device providing a hermetic seal so as to provide, with the first closure and the at least one wall, a sterile barrier. Other embodiments are also disclosed.

Abstract: There is disclosed a method of procuring allograft tissue. In an embodiment, the method includes inserting an allograft through an opening into a procurement extraction container, sealing the opening, adding extraction fluid through a second opening, agitating the fluid, collecting the fluid for detection of contamination, and sealing the second opening. There is disclosed a procurement extraction container. In one embodiment, the container includes a wall defining an interior to receive the allograft, an opening into the interior, sized to receive the allograft, a closure device for the opening, the closure device providing a hermetic seal, a second opening providing a second passageway into the interior, the second opening having a connector interface and a second closure device providing a hermetic seal so as to provide, with the first closure and the at least one wall, a sterile barrier. Other embodiments are also disclosed.

Abstract: Embodiments of the present invention encompass compositions containing an adipose derived carrier, matrix, or filler, in some cases optionally in combination with bone particles or other granular materials or substances, for delivery to a human patient. Methods of manufacture and use of such adipose derived compositions are also disclosed.

Abstract: There is disclosed a method of combining mesenchymal stem cells (MSCs) with an osteochondral allograft. In an embodiment, the method includes obtaining adipose tissue having MSCs with unwanted cells. The tissue is digested to form a cell suspension having MSCs and unwanted cells. The suspension is added to seed the allograft. The MSCs are allowed to attach to the allograft. There is disclosed an allograft product including MSCs with an osteochondral allograft. There is disclosed a method of combining MSCs with decellularized, morselized cartilage. In an embodiment, the method includes obtaining adipose tissue having MSCs with unwanted cells. The tissue is digested to form a cell suspension having MSCs and unwanted cells. The suspension is added to seed the cartilage. The MSCs are allowed to attach to the cartilage to. There is disclosed an allograft product including MSCs with morselized cartilage. Other embodiments are also disclosed.

Abstract: There is disclosed a system for cleaning and disinfecting allograft material. In an embodiment, the system includes a sterile bag and a motorized paddle blender. The sterile bag having an outer wall configured to withstand blending forces, at least one paddle contact section, and an allograft retaining section separate from the at least one paddle contact section. The motorized paddle blender having at least one paddle configured to apply blending forces on the sterile bag on the at least one paddle contact area, a motorized portion to actuate the at least one paddle, and a door component configured to hold the sterile bag adjacent the at least one paddle. The at least one paddle and the allograft retaining section are configured to prevent the paddle from contacting the allograft material when the blending forces are applied on the sterile bag on the at least one paddle contact area.

Abstract: There is disclosed a method of procuring allograft tissue. In an embodiment, the method includes inserting an allograft through an opening into a procurement extraction container, sealing the opening, adding extraction fluid through a second opening, agitating the fluid, collecting the fluid for detection of contamination, and sealing the second opening. There is disclosed a procurement extraction container. In one embodiment, the container includes a wall defining an interior to receive the allograft, an opening into the interior, sized to receive the allograft, a closure device for the opening, the closure device providing a hermetic seal, a second opening providing a second passageway into the interior, the second opening having a connector interface and a second closure device providing a hermetic seal so as to provide, with the first closure and the at least one wall, a sterile barrier. Other embodiments are also disclosed.

Abstract: There is disclosed a method of procuring allograft tissue. In an embodiment, the method includes inserting an allograft through an opening into a procurement extraction container, sealing the opening, adding extraction fluid through a second opening, agitating the fluid, collecting the fluid for detection of contamination, and sealing the second opening. There is disclosed a procurement extraction container. In one embodiment, the container includes a wall defining an interior to receive the allograft, an opening into the interior, sized to receive the allograft, a closure device for the opening, the closure device providing a hermetic seal, a second opening providing a second passageway into the interior, the second opening having a connector interface and a second closure device providing a hermetic seal so as to provide, with the first closure and the at least one wall, a sterile barrier. Other embodiments are also disclosed.

Abstract: Embodiments of the present invention encompass graft assemblies, and methods for their use and manufacture. An exemplary bone graft assembly includes first and second bone pieces having respective mating features which, when combined, define non-uniform press fit. Related embodiments encompass graft assemblies having enclosed or hidden mating features.

Abstract: Embodiments of the present invention encompass anti-adhesion wound dressings including patches made from amnion tissue obtained from human birth tissue. Exemplary amnion patches can be fabricated by folding a section of amnion over on itself with the epithelial layer on the outside of the folded patch and the fibroblast layer on the inside of the folded patch. Optionally, individual amnion tissue pieces can be sandwiched together to provide a multi-layer patch. Sufficient pressure is applied to the layered amnion to cause adherence between opposing faces of the fibroblast layers. The pressed fibroblast layers provide mechanical strength to hold the amnion patch together with the epithelial layers on the outsides of the amnion patch.

Abstract: There is disclosed a constant force compression tool for measuring a compressible material. In an embodiment, the tool includes a handle having a passageway receiving a plunger. The tool includes a biasing member in communication with the plunger and the handle. The tool includes a retaining element in communication with the plunger and the handle. There is disclosed a system for measuring a compressible material with a measurement tube and a constant force compression tool. There is disclosed a method of measuring a compressible material. In an embodiment, the method includes selecting a measurement tube. The method includes placing a compressible material into the measurement tube. The method includes pressing a plunger against the compressible material. The method includes monitoring an indicator to determine a full volume of compressible material between the end of the plunger and the conical tube. Other embodiments are also disclosed.

Abstract: There are disclosed systems and methods of delivering sterile fluids aseptically to a sealed canister. In an embodiment, a includes a canister having an inlet, a vent, and a drain outlet, a reagent manifold in communication with the inlet, a bubbler in communication with the vent, and a fluid communicator from the drain outlet. In another embodiment, a method includes providing a canister having an inlet, a vent, and a drain outlet; selectively providing reagents to the canister from a reagent manifold in communication with the inlet, allowing excess gasses to leave the canister with a bubbler in communication with the vent, and selectively purging the reagents from the canister through the drain outlet. Other embodiments are also disclosed.

Abstract: Apparatus for treating allografts, having a sonication tank configured to transmit ultrasonic energy to the interior of the tank; a treatment canister rotatably positioned in said sonication tank, and configured to receive allografts therein; and a treatment fluid source in fluid communication with said treatment canister. Methods of treating allografts and methods for determining microbial contamination using the apparatus.

Abstract: A method for determining microbial contamination of allograft products comprises providing an extraction vessel having an outer container and a removable inner basket, place the allograft product into the inner basket, providing an abstraction fluid in the outer container of the extraction vessel, positioning the inner basket in the outer container, so that the allograft product and the inner basket is submerged in the extraction fluid and agitating the extraction fluid for a predetermined amount of time and analyzing the extraction fluid from microbial contamination.

Abstract: A method for determining microbial contamination of allograft products includes providing an extraction vessel having an outer container and a removable inner basket, placing the allograft product into the inner basket, providing an extraction fluid in the outer container of the extraction vessel, positioning the inner basket in the outer container, so that the allograft product and the inner basket are submerged in the extraction fluid and agitating the extraction fluid for a predetermined amount of time and analyzing the extraction fluid for microbial contamination.

Abstract: Embodiments of the present invention encompass anti-adhesion wound dressings including patches made from amnion tissue obtained from human birth tissue. Exemplary amnion patches can be fabricated by folding a section of amnion over on itself with the epithelial layer on the outside of the folded patch and the fibroblast layer on the inside of the folded patch. Optionally, individual amnion tissue pieces can be sandwiched together to provide a multi-layer patch. Sufficient pressure is applied to the layered amnion to cause adherence between opposing faces of the fibroblast layers. The pressed fibroblast layers provide mechanical strength to hold the amnion patch together with the epithelial layers on the outsides of the amnion patch.

Abstract: There are disclosed systems and methods of delivering sterile fluids aseptically to a sealed canister. In an embodiment, a includes a canister having an inlet, a vent, and a drain outlet, a reagent manifold in communication with the inlet, a bubbler in communication with the vent, and a fluid communicator from the drain outlet. In another embodiment, a method includes providing a canister having an inlet, a vent, and a drain outlet; selectively providing reagents to the canister from a reagent manifold in communication with the inlet, allowing excess gasses to leave the canister with a bubbler in communication with the vent, and selectively purging the reagents from the canister through the drain outlet. Other embodiments are also disclosed.

Abstract: Apparatus for treating allografts, having a sonication tank configured to transmit ultrasonic energy to the interior of the tank; a treatment canister rotatably positioned in said sonication tank, and configured to receive allografts therein; and a treatment fluid source in fluid communication with said treatment canister. Methods of treating allografts and methods for determining microbial contamination using the apparatus.