Patents Assigned to Amano Enzyme Inc.
  • Publication number: 20240052323
    Abstract: An object of the present invention is to find a new mutation effective for improving transglutaminase, and to provide a highly useful modified transglutaminase. Disclosed is a highly useful modified transglutaminase having an amino acid substitution that results in a reduction in temperature stability, an improvement in heat resistance, an improvement in oxidation resistance, an improvement in reactivity, or conversion into deamidase.
    Type: Application
    Filed: June 21, 2023
    Publication date: February 15, 2024
    Applicant: AMANO ENZYME INC.
    Inventor: Kazunori Yoshida
  • Patent number: 11859222
    Abstract: The present invention has a purpose of providing a novel ?-galactosidase enzyme useful for the production of oligosaccharides. Disclosed is a ?-galactosidase enzyme comprising the amino acid sequence of any one of SEQ ID NOs: 1 to 4 or an amino acid sequence that is 80% or more identical to said amino acid sequence.
    Type: Grant
    Filed: October 7, 2022
    Date of Patent: January 2, 2024
    Assignee: AMANO ENZYME INC.
    Inventors: Yukiko Hoshi, Masamichi Okada, Akio Horii, Masayuki Hojo
  • Publication number: 20230413869
    Abstract: It is an object of the present invention to provide a browning agent for food product, which is capable of browning a food product in a step of heat-cooking the food product. According to the present invention, provided are: a browning agent for food product, comprising glycosidase, polyphenol oxidase or cellulase; a food product comprising the browning agent for food product; and a method for browning a food product, comprising adding glycosidase, polyphenol oxidase or cellulase to the food product, and then heating the food product.
    Type: Application
    Filed: November 15, 2021
    Publication date: December 28, 2023
    Applicant: AMANO ENZYME INC.
    Inventors: Ryosuke Tanaka, Hiroyuki Yachi
  • Publication number: 20230407356
    Abstract: This invention provides a method for producing allulose using an allulose-producing enzyme having high pH stability in a low-pH region. The method comprises a step of allowing a polypeptide consisting of the amino acid sequence shown in SEQ ID NO: 6 or a polypeptide consisting of a sequence having 90% or higher identity to the amino acid sequence shown in SEQ ID NO: 6 and capable of producing allulose to react with fructose under acidic conditions in which pH is less than 6.
    Type: Application
    Filed: October 8, 2021
    Publication date: December 21, 2023
    Applicant: AMANO ENZYME INC.
    Inventors: Kiyota Sakai, Hiroyuki Yachi, Kensuke Yuki, Masahiro Kamon
  • Patent number: 11833192
    Abstract: A method for improving intestinal flora increases the number of beneficial bacteria such as lactic acid bacteria and Bifidobacterium to improve intestinal flora by using an enzyme. The method involves providing a protease, which is a polypeptide having an amino acid sequence shown in SEQ ID NO: 1 that can increase beneficial bacteria such as lactic acid bacteria and Bifidobacterium in intestines to exert an excellent effect of improving intestinal flora.
    Type: Grant
    Filed: October 5, 2021
    Date of Patent: December 5, 2023
    Assignees: AMANO ENZYME INC., HIROSHIMA UNIVERSITY
    Inventors: Manabu Kuroda, Shotaro Yamaguchi, Norihisa Kato
  • Publication number: 20230279454
    Abstract: The usefulness of ?-galactosidases derived from Bacillus circulans is further enhanced. A modified ?-galactosidase in which one or more amino acids selected from the group consisting of proline 182 (P182), tyrosine 187 (Y187), serine 188 (S188), tryptophan 405 (W405), alanine 406 (A406), glutamine 407 (Q407), tyrosine 449 (Y449), threonine 483 (T483), serine 512 (S512), serine 531 (S531), threonine 533 (T533), serine 534 (S534), asparagine 550 (N550), glutamine 551 (Q551), tryptophan 593 (W593), tyrosine 598 (Y598), proline 602 (P602), proline 604 (P604), tyrosine 609 (Y609), lysine 612 (K612), and tyrosine 615 (Y615), or an amino acid(s) corresponding thereto, has/have been substituted by other amino acid in a ?-galactosidase consisting of the amino acid sequence of any of SEQ ID NOs. 1 to 4 or an amino acid sequence having 90% or more identity to the amino acid sequence set forth in any of SEQ ID NOs. 1 to 4.
    Type: Application
    Filed: December 14, 2022
    Publication date: September 7, 2023
    Applicant: AMANO ENZYME INC.
    Inventors: Genichi Nakamura, Yuko Kuritani, Satoru Ishihara, Tetsuya Takahashi
  • Patent number: 11718837
    Abstract: To find a novel lipase suitable for processing of oils and fats and to use and apply the lipase. Provided are a novel lipase including an amino acid sequence having 90% or more identity with an amino acid sequence of SEQ ID NO: 1 or 2, and an enzyme preparation containing the same as an active ingredient. The enzyme preparation is used for modifying and improving the physical properties of oils and fats.
    Type: Grant
    Filed: July 29, 2020
    Date of Patent: August 8, 2023
    Assignee: AMANO ENZYME INC.
    Inventors: Tomohiro Kimura, Tomoya Ryoki, Yusuke Tanaka, Naoto Katsumata, Yukihide Sato
  • Patent number: 11685914
    Abstract: A technique of using a compositional innovation of a dry enzyme composition to improve the stability of a protein deamidase composition enables improvement of the stability of a dry protein deamidase composition by making both a protein deamidase and magnesium chloride coexist in a dry enzyme composition. The technique involves making adjustments so that the pH is at least 2 and less than 5 when the composition is dissolved in water at a concentration of 1 w/v %.
    Type: Grant
    Filed: September 6, 2018
    Date of Patent: June 27, 2023
    Assignee: AMANO ENZYME INC.
    Inventor: Takakiyo Obara
  • Publication number: 20230193233
    Abstract: The present invention has a purpose of providing a novel ?-galactosidase enzyme useful for the production of oligosaccharides. Disclosed is a ?-galactosidase enzyme comprising the amino acid sequence of any one of SEQ ID NOs: 1 to 4 or an amino acid sequence that is 80% or more identical to said amino acid sequence.
    Type: Application
    Filed: October 7, 2022
    Publication date: June 22, 2023
    Applicant: AMANO ENZYME INC.
    Inventors: Yukiko Hoshi, Masamichi Okada, Akio Horii, Masayuki Hojo
  • Publication number: 20230114818
    Abstract: The object of the present invention is to provide highly safe collagenase that is useful for food or medical use, and the use thereof. Provided is an enzyme agent comprising, as an active ingredient, collagenase having an amino acid sequence having an identity of 90% or more to the amino acid sequence as set forth in SEQ ID NO: 1. This enzyme agent is useful for production of a collagen tripeptide or tenderization of edible meat.
    Type: Application
    Filed: March 30, 2021
    Publication date: April 13, 2023
    Applicant: AMANO ENZYME INC.
    Inventors: Masahiro Kamon, Kensuke Yuki, Hiroyuki Yachi
  • Patent number: 11555214
    Abstract: The usefulness of ?-galactosidases derived from Bacillus circulans is further enhanced. A modified ?-galactosidase in which one or more amino acids selected from the group consisting of proline 182 (P182), tyrosine 187 (Y187), serine 188 (S188), tryptophan 405 (W405), alanine 406 (A406), glutamine 407 (Q407), tyrosine 449 (Y449), threonine 483 (T483), serine 512 (S512), serine 531 (S531), threonine 533 (T533), serine 534 (S534), asparagine 550 (N550), glutamine 551 (Q551), tryptophan 593 (W593), tyrosine 598 (Y598), proline 602 (P602), proline 604 (P604), tyrosine 609 (Y609), lysine 612 (K612), and tyrosine 615 (Y615), or an amino acid(s) corresponding thereto, has/have been substituted by other amino acid in a ?-galactosidase consisting of the amino acid sequence of any of SEQ ID NOs. 1 to 4 or an amino acid sequence having 90% or more identity to the amino acid sequence set forth in any of SEQ ID NOs. 1 to 4.
    Type: Grant
    Filed: September 18, 2019
    Date of Patent: January 17, 2023
    Assignee: AMANO ENZYME INC.
    Inventors: Genichi Nakamura, Yuko Kuritani, Satoru Ishihara, Tetsuya Takahashi
  • Patent number: 11549130
    Abstract: An object of the present invention is to provide an enzyme showing random transesterification ability, which is suitable for food applications. Provided is a random transesterification lipase having high heat resistance in oils and fats.
    Type: Grant
    Filed: December 27, 2018
    Date of Patent: January 10, 2023
    Assignee: AMANO ENZYME INC.
    Inventors: Hiroki Fujioka, Saki Norifune, Tatsuya Hamada, Tetsuya Takahashi, Yusuke Tanaka, Toru Katase
  • Publication number: 20230002750
    Abstract: A method for preparing a stromal vascular fraction from an adipose tissue, including a step of treating an adipose tissue with an enzyme solution containing a collagenase and a neutral protease, preferably an enzyme solution free of clostripain and thermolysin, and showing not less than 1 U neutral protease activity with respect to 10,000 U collagenase activity, and recovering cells is provided by the present invention.
    Type: Application
    Filed: December 3, 2020
    Publication date: January 5, 2023
    Applicants: AMANO ENZYME INC., JICHI MEDICAL UNIVERSITY
    Inventors: Kazuhiro FURUKAWA, Yuki ISHIGAKI, Kotaro YOSHIMURA, Takako SHIRADO, Natsumi SAITO, Rintaro ASAHI, Masanori MORI
  • Patent number: 11517613
    Abstract: A lactase bulk powder and a lactase preparation both have improved storage stability. The stability of a lactase bulk powder is improved by regulating the acceptable amount of glucose and/or galactose, generated during manufacturing the lactase bulk powder, within a preset range. The lactase bulk powder includes lactase, galactose and/or glucose. The total amount of galactose and glucose is more than 0 ?mol and not more than 50 ?mol per 100,000 U of lactase. The lactase preparation has the lactase bulk powder as an active ingredient.
    Type: Grant
    Filed: May 31, 2018
    Date of Patent: December 6, 2022
    Assignee: AMANO ENZYME INC.
    Inventors: Manabu Kuroda, Masayuki Hojo
  • Patent number: 11512299
    Abstract: The present invention has a purpose of providing a novel ?-galactosidase enzyme useful for the production of oligosaccharides. Disclosed is a ?-galactosidase enzyme comprising the amino acid sequence of any one of SEQ ID NOs: 1 to 4 or an amino acid sequence that is 80% or more identical to said amino acid sequence.
    Type: Grant
    Filed: December 27, 2016
    Date of Patent: November 29, 2022
    Assignee: AMANO ENZYME INC.
    Inventors: Yukiko Hoshi, Masamichi Okada, Akio Horii, Masayuki Hojo
  • Publication number: 20220364070
    Abstract: It is necessary to establish a purification method effective for the removal of endotoxin, in order to obtain thermolysin containing a reduced amount of contaminant endotoxin. On the premise of the establishment, accurate measurement of the amount of endotoxin that contaminates thermolysin is required. The present invention addresses the problem of providing a novel measurement method which enables accurate measurement of the amount of endotoxin that contaminates thermolysin. The present invention also addresses the problem of providing thermolysin containing a reduced amount of contaminant thermolysin, and the use thereof. There is provided thermolysin containing contaminant endotoxin in an amount of 1 EU/mg or less. There is also provided a method for measuring endotoxin that contaminates thermolysin, the method including pretreatment of deactivating the thermolysin.
    Type: Application
    Filed: June 2, 2022
    Publication date: November 17, 2022
    Applicant: AMANO ENZYME INC.
    Inventors: Takafumi Koyama, Hiroki Ido, Shotaro Yamaguchi
  • Patent number: 11414654
    Abstract: An object of the present invention is to provide an enzyme preparation useful for reducing purine bodies, especially, in beer or beer-based beverages, and use thereof. Provided is a nucleosidase preparation having an activity ratio (U/U), which is guanine deaminase activity per nucleosidase activity, of 0.4 or less.
    Type: Grant
    Filed: January 30, 2019
    Date of Patent: August 16, 2022
    Assignees: AMANO ENZYME INC., SUNTORY HOLDINGS LIMITED
    Inventors: Takakiyo Obara, Keita Okuda, Tomoko Fujimura, Ikuma Mizuguchi, Tomoyuki Nakahama
  • Patent number: 11359190
    Abstract: A thermolysin solution has further improved stability as a result of the thermolysin solution containing thermolysin at a concentration that is 0.1 mg/mL or higher and the thermolysin solution having a pH that is adjusted to higher than 9.0. The thermolysin solution preferably has a pH in the range of 9.5-11.5.
    Type: Grant
    Filed: July 30, 2018
    Date of Patent: June 14, 2022
    Assignee: AMANO ENZYME INC.
    Inventor: Kazuhiro Furukawa
  • Publication number: 20220142210
    Abstract: Described herein are stable protein solutions that have a pH of from about 3.5 to about 7.0 and are stable against precipitation of the protein, as well as methods of making such stable protein solutions, and beverages and beverages additives for human or animal consumption comprising such stable protein solutions. These stable protein solutions comprise a protein, a stabilizer, and a protein deamidating enzyme.
    Type: Application
    Filed: February 25, 2020
    Publication date: May 12, 2022
    Applicants: AMANO ENZYME USA CO., LTD., AMANO ENZYME INC.
    Inventors: Theodore S. LIOUTAS, Keita OKUDA
  • Publication number: 20220117259
    Abstract: Methods including performing (I) a step of adding an acid to a water dispersion of a pea ground product and collecting the precipitate, and (II) a step of conducting an enzyme reaction by adding protein deamidase, in this order or reverse order, are useful for improving the solubility of a modified pea protein.
    Type: Application
    Filed: December 28, 2021
    Publication date: April 21, 2022
    Applicants: AJINOMOTO CO., INC., AMANO ENZYME INC.
    Inventors: Hiroshi TAKAYANAGI, Hiroyuki NAKAGOSHI, Rikiya ISHIDA