Patents Assigned to AmberGen, Inc.
  • Patent number: 12013392
    Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.
    Type: Grant
    Filed: September 2, 2021
    Date of Patent: June 18, 2024
    Assignee: AMBERGEN, INC.
    Inventors: Mark J Lim, Vladislav B Bergo, Kenneth J Rothschild
  • Publication number: 20240159749
    Abstract: Methods and compositions are described for the diagnosis of primary biliary cirrhosis. Novel autoantigens are described for use in assays which employ test samples from individuals.
    Type: Application
    Filed: November 28, 2023
    Publication date: May 16, 2024
    Applicants: AMBERGEN, INC., THE GENERAL HOSPITAL CORPORATION
    Inventors: MARK J. LIM, HEATHER P. OSTENDORFF, KENNETH J. ROTHSCHILD, DONALD B. BLOCH
  • Patent number: 11940447
    Abstract: The field of this invention relates to immunohistochemistry (IHC) and in situ hybridization (ISH) for the targeted detection and mapping of biomolecules (e.g., proteins and miRNAs) in tissues or cells for example, for research use and for clinical use such by pathologists (e.g., biomarker analyses of a resected tumor or tumor biopsy). In particular, the use of mass spectrometric imaging (MSI) as a mode to detect and map the biomolecules in tissues or cells for example. More specifically, the field of this invention relates to photocleavable mass-tag reagents which are attached to probes such as antibodies and nucleic acids and used to achieve multiplex immunohistochemistry and in situ hybridization, with MSI as the mode of detection/readout. Probe types other than antibodies and nucleic acids are also covered in the field of invention, including but not limited to carbohydrate-binding proteins (e.g., lectins), receptors and ligands.
    Type: Grant
    Filed: July 12, 2022
    Date of Patent: March 26, 2024
    Assignee: AMBERGEN, INC.
    Inventors: Mark J. Lim, Gargey Yagnik, Kenneth J. Rothschild
  • Patent number: 11906527
    Abstract: The field of this invention relates to immunohistochemistry (IHC) and in situ hybridization (ISH) for the targeted detection and mapping of biomolecules (e.g., proteins and miRNAs) in tissues or cells for example, for research use and for clinical use such by pathologists (e.g., biomarker analyses of a resected tumor or tumor biopsy). In particular, the use of mass spectrometric imaging (MSI) as a mode to detect and map the biomolecules in tissues or cells for example. More specifically, the field of this invention relates to photocleavable mass-tag reagents which are attached to probes such as antibodies and nucleic acids and used to achieve multiplex immunohistochemistry and in situ hybridization, with MSI as the mode of detection/readout. Probe types other than antibodies and nucleic acids are also covered in the field of invention, including but not limited to carbohydrate-binding proteins (e.g., lectins), receptors and ligands.
    Type: Grant
    Filed: August 11, 2021
    Date of Patent: February 20, 2024
    Assignee: AMBERGEN, INC.
    Inventors: Mark J. Lim, Gargey Yagnik, Kenneth J. Rothschild
  • Patent number: 11885802
    Abstract: Methods and compositions are described for the diagnosis of primary biliary cirrhosis. Novel autoantigens are described for use in assays which employ test samples from individuals.
    Type: Grant
    Filed: March 24, 2017
    Date of Patent: January 30, 2024
    Assignees: AMBERGEN, INC., MASSACHUSETTS GENERAL HOSPITAL
    Inventors: Mark J. Lim, Heather P. Ostendorff, Kenneth J. Rothschild, Donald B. Bloch
  • Patent number: 11846634
    Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.
    Type: Grant
    Filed: April 3, 2020
    Date of Patent: December 19, 2023
    Assignee: AMBERGEN, INC.
    Inventors: Mark J Lim, Vladislav B Bergo, Kenneth J Rothschild
  • Patent number: 11789027
    Abstract: The field of this invention relates to immunohistochemistry (IHC) and in situ hybridization (ISH) for the targeted detection and mapping of biomolecules (e.g., proteins and miRNAs) in tissues or cells for example, for research use and for clinical use such by pathologists (e.g., biomarker analyses of a resected tumor or tumor biopsy). In particular, the use of mass spectrometric imaging (MSI) as a mode to detect and map the biomolecules in tissues or cells for example. The field of this invention relates to photocleavable mass-tag reagents which are attached to probes such as antibodies and nucleic acids and used to achieve multiplex immunohistochemistry and in situ hybridization, with MSI as the mode of detection/readout, and also encompasses multi-omic MSI procedures, where MSI of photocleavable mass-tag probes is combined with other modes of MSI.
    Type: Grant
    Filed: August 11, 2021
    Date of Patent: October 17, 2023
    Assignee: AmberGen, Inc.
    Inventors: Mark J. Lim, Gargey Yagnik, Kenneth J. Rothschild
  • Patent number: 11782056
    Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.
    Type: Grant
    Filed: April 3, 2020
    Date of Patent: October 10, 2023
    Assignee: AmberGen, Inc.
    Inventors: Mark J Lim, Vladislav B Bergo, Kenneth J Rothschild
  • Patent number: 10088474
    Abstract: Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.
    Type: Grant
    Filed: October 6, 2015
    Date of Patent: October 2, 2018
    Assignee: AmberGen, Inc.
    Inventors: Mark J Lim, Kenneth J Rothschild
  • Patent number: 10060912
    Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.
    Type: Grant
    Filed: October 3, 2016
    Date of Patent: August 28, 2018
    Assignee: AmberGen, Inc.
    Inventors: Mark J Lim, Vladislav B Bergo, Kenneth J Rothschild
  • Patent number: 9910034
    Abstract: Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.
    Type: Grant
    Filed: January 14, 2015
    Date of Patent: March 6, 2018
    Assignee: AmberGen, Inc.
    Inventors: Mark J. Lim, Kenneth J. Rothschild
  • Patent number: 9523680
    Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.
    Type: Grant
    Filed: June 29, 2011
    Date of Patent: December 20, 2016
    Assignee: AmberGen, Inc.
    Inventors: Mark J. Lim, Kenneth J. Rothschild
  • Patent number: 9513285
    Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.
    Type: Grant
    Filed: December 17, 2013
    Date of Patent: December 6, 2016
    Assignee: AmberGen, Inc.
    Inventors: Mark J. Lim, Vladislav B. Bergo, Kenneth J. Rothschild
  • Patent number: 9334530
    Abstract: Methods of amplifying nucleic acid on a solid support are described. Beads and template, each in known concentrations, are employed so a range of template to bead ratios can be exploited. Where the beads contain primers, the template can be amplified. After amplification, non-covalently bound template is removed, so as to leave beads with extended primers (or beads with primers that were not extended).
    Type: Grant
    Filed: May 13, 2010
    Date of Patent: May 10, 2016
    Assignee: AmberGen, Inc.
    Inventors: Mark J. Lim, Kenneth J. Rothschild
  • Patent number: 8932879
    Abstract: Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.
    Type: Grant
    Filed: October 30, 2008
    Date of Patent: January 13, 2015
    Assignee: Ambergen, Inc.
    Inventors: Mark J. Lim, Kenneth J. Rothschild
  • Patent number: 8906700
    Abstract: Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.
    Type: Grant
    Filed: July 19, 2012
    Date of Patent: December 9, 2014
    Assignee: Ambergen, Inc.
    Inventors: Mark J. Lim, Kenneth J. Rothschild
  • Patent number: 8852956
    Abstract: Methods and compositions are described for the diagnosis of primary biliary cirrhosis. Novel autoantigens are described for use in assays which employ test samples from individuals.
    Type: Grant
    Filed: October 5, 2010
    Date of Patent: October 7, 2014
    Assignees: Ambergen, Inc., Massachusetts General Hospital
    Inventors: Mark J. Lim, Heather P. Ostendorf, Kenneth J. Rothschild, Donald B. Bloch
  • Publication number: 20140235471
    Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.
    Type: Application
    Filed: December 17, 2013
    Publication date: August 21, 2014
    Applicant: Ambergen, Inc.
    Inventors: Vladislav B. Bergo, Mark J. Lim, Kenneth J. Rothschild
  • Publication number: 20140081007
    Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes.
    Type: Application
    Filed: August 22, 2013
    Publication date: March 20, 2014
    Applicant: Ambergen, Inc.
    Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik
  • Patent number: 8507648
    Abstract: This invention relates to the detection and analysis by mass spec of nascent proteins, and in particular truncated proteins, translated within cellular or cell-free translation systems. N-terminal and C-terminal epitopes introduced into these nascent proteins permit rapid and efficient isolation, as well as mass difference.
    Type: Grant
    Filed: August 4, 2010
    Date of Patent: August 13, 2013
    Assignee: Ambergen, Inc.
    Inventors: Alex Garvin, Sadanand Gite, Vladislav B. Bergo, Kenneth J. Rothschild