Patents Assigned to AmberGen, Inc.
  • Patent number: 10088474
    Abstract: Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.
    Type: Grant
    Filed: October 6, 2015
    Date of Patent: October 2, 2018
    Assignee: AmberGen, Inc.
    Inventors: Mark J Lim, Kenneth J Rothschild
  • Patent number: 10060912
    Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.
    Type: Grant
    Filed: October 3, 2016
    Date of Patent: August 28, 2018
    Assignee: AmberGen, Inc.
    Inventors: Mark J Lim, Vladislav B Bergo, Kenneth J Rothschild
  • Patent number: 9910034
    Abstract: Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.
    Type: Grant
    Filed: January 14, 2015
    Date of Patent: March 6, 2018
    Assignee: AmberGen, Inc.
    Inventors: Mark J. Lim, Kenneth J. Rothschild
  • Patent number: 9523680
    Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.
    Type: Grant
    Filed: June 29, 2011
    Date of Patent: December 20, 2016
    Assignee: AmberGen, Inc.
    Inventors: Mark J. Lim, Kenneth J. Rothschild
  • Patent number: 9513285
    Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.
    Type: Grant
    Filed: December 17, 2013
    Date of Patent: December 6, 2016
    Assignee: AmberGen, Inc.
    Inventors: Mark J. Lim, Vladislav B. Bergo, Kenneth J. Rothschild
  • Patent number: 9334530
    Abstract: Methods of amplifying nucleic acid on a solid support are described. Beads and template, each in known concentrations, are employed so a range of template to bead ratios can be exploited. Where the beads contain primers, the template can be amplified. After amplification, non-covalently bound template is removed, so as to leave beads with extended primers (or beads with primers that were not extended).
    Type: Grant
    Filed: May 13, 2010
    Date of Patent: May 10, 2016
    Assignee: AmberGen, Inc.
    Inventors: Mark J. Lim, Kenneth J. Rothschild
  • Patent number: 8932879
    Abstract: Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.
    Type: Grant
    Filed: October 30, 2008
    Date of Patent: January 13, 2015
    Assignee: Ambergen, Inc.
    Inventors: Mark J. Lim, Kenneth J. Rothschild
  • Patent number: 8906700
    Abstract: Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.
    Type: Grant
    Filed: July 19, 2012
    Date of Patent: December 9, 2014
    Assignee: Ambergen, Inc.
    Inventors: Mark J. Lim, Kenneth J. Rothschild
  • Patent number: 8852956
    Abstract: Methods and compositions are described for the diagnosis of primary biliary cirrhosis. Novel autoantigens are described for use in assays which employ test samples from individuals.
    Type: Grant
    Filed: October 5, 2010
    Date of Patent: October 7, 2014
    Assignees: Ambergen, Inc., Massachusetts General Hospital
    Inventors: Mark J. Lim, Heather P. Ostendorf, Kenneth J. Rothschild, Donald B. Bloch
  • Publication number: 20140235471
    Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.
    Type: Application
    Filed: December 17, 2013
    Publication date: August 21, 2014
    Applicant: Ambergen, Inc.
    Inventors: Vladislav B. Bergo, Mark J. Lim, Kenneth J. Rothschild
  • Publication number: 20140081007
    Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes.
    Type: Application
    Filed: August 22, 2013
    Publication date: March 20, 2014
    Applicant: Ambergen, Inc.
    Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik
  • Patent number: 8507648
    Abstract: This invention relates to the detection and analysis by mass spec of nascent proteins, and in particular truncated proteins, translated within cellular or cell-free translation systems. N-terminal and C-terminal epitopes introduced into these nascent proteins permit rapid and efficient isolation, as well as mass difference.
    Type: Grant
    Filed: August 4, 2010
    Date of Patent: August 13, 2013
    Assignee: Ambergen, Inc.
    Inventors: Alex Garvin, Sadanand Gite, Vladislav B. Bergo, Kenneth J. Rothschild
  • Patent number: 8486634
    Abstract: Methods of amplifying nucleic acid are described. Primers on solid support, e.g. a population of beads, are employed. A population of nucleic acid template molecules, wherein the nucleic acid template molecules have been treated with bisulfite, is amplified so as to create loaded beads comprising amplified nucleic acid.
    Type: Grant
    Filed: November 6, 2008
    Date of Patent: July 16, 2013
    Assignee: Ambergen, Inc.
    Inventors: Mark J. Lim, Kenneth J. Rothschild
  • Patent number: 8288098
    Abstract: This invention relates to agents and conjugates to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed.
    Type: Grant
    Filed: October 27, 2011
    Date of Patent: October 16, 2012
    Assignee: AmberGen, Inc.
    Inventors: Kenneth J. Rothschild, Sanjay M. Sonar, Jerzy Olejnik
  • Patent number: 8278045
    Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)dyes.
    Type: Grant
    Filed: February 9, 2011
    Date of Patent: October 2, 2012
    Assignee: Ambergen, Inc.
    Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik
  • Publication number: 20120244562
    Abstract: Methods and compositions are described for the diagnosis of primary biliary cirrhosis. Novel autoantigens are described for use in assays which employ test samples from individuals.
    Type: Application
    Filed: October 5, 2010
    Publication date: September 27, 2012
    Applicants: AMBERGEN, INC., MASSACHUSETTS GENERAL HOSPITIAL
    Inventors: Mark J. Lim, Heather P. Ostendorf, Kenneth J. Rothschild, Donald B. Bloch
  • Patent number: 8148062
    Abstract: The invention is directed to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives, or substances recognized by the protein synthesizing machinery. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system. The invention also comprises proteins prepared using misaminoacylated tRNAs which can be utilized in pharmaceutical compositions for the treatment of diseases and disorders in humans and other mammals, and kits which may used for the detection of diseases and disorders.
    Type: Grant
    Filed: December 14, 2006
    Date of Patent: April 3, 2012
    Assignee: Ambergen, Inc.
    Inventors: Kenneth J. Rothschild, Sanjay M. Sonar, Jerzy Olejnik
  • Patent number: 8114587
    Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes.
    Type: Grant
    Filed: June 23, 2005
    Date of Patent: February 14, 2012
    Assignee: Ambergen, Inc.
    Inventors: Sadanand Gite, Jennifer A. McCullough, Kenneth J. Rothschild
  • Patent number: 7897335
    Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes.
    Type: Grant
    Filed: June 18, 2002
    Date of Patent: March 1, 2011
    Assignee: Ambergen, Inc.
    Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik
  • Patent number: 7807407
    Abstract: This invention relates to the detection and analysis by mass spec of nascent proteins, and in particular truncated proteins, translated within cellular or cell-free translation systems. N-terminal and C-terminal epitopes introduced into these nascent proteins permit rapid and efficient isolation, as well as a mass difference.
    Type: Grant
    Filed: July 30, 2004
    Date of Patent: October 5, 2010
    Assignee: AmberGen, Inc.
    Inventors: Alex M. Garvin, Sadanand Gite, Vladislav B. Bergo, Kenneth J. Rothschild