Abstract: Fluorescence reader (10) for an optical assay arrangement comprising a polymeric sample substrate (1) having a reaction site-surface and a substrate surface (3), the fluorescence reader comprising a light source (5) arranged to illuminate the reaction site-surface through the substrate surface, and a detector device (6) arranged to detect fluorescent light emitted from said reaction site-surface and transmitted through the substrate surface, the substrate surface provided with total-internal-reflection suppressing means (15).

Abstract: Manufacturing of miniaturized three-dimensional electric components are presented, as well as components manufactured by the methods. The manufacturing methods comprise micro-replication of at least one master structure, e.g. via a mould structure, in at least one polymer layer onto which layer at least one conductive path is provided.

Abstract: Method of manufacturing a microscale nozzle, comprising the steps of forming a microscale channel in the top surface of a substrate, said microscale channel comprising an inlet end and a nozzle-end, depositing a nozzle-forming layer in a section of the microscale channel, and removing material from the substrate at the nozzle-end of the microscale channel to expose at least a portion of said nozzle-forming layer. The manufactured microscale nozzle may be used for transferring a liquid sample form a microchip fluidic system into an external analytical device.

Abstract: The invention comprises a polymeric microarray support (1) for an optical assay arrangement (2) comprising optical means (3, 4, 6) for detection of light emitted from the support. The microarray support is provided with microfeatures comprising a surface enlarging pattern (5), i.e. grooves having a selected depth (8). The depth is selected such that the sum of the depth and of the variations in the thickness (7) of the support substantially corresponds to the depth of focus of the optical means.

Abstract: Optical reader (33, 43, 53, 63, 73, 83) for an optical assay arrangement comprising a polymeric sample substrate (1) having a reaction site-area (5) provided with protruding microstructures (2), the optical reader comprising a light source (6, 11) for illuminating the reaction site-area (5), and a detector device (7, 12) for detecting light emitted from said reaction site-area.

Abstract: An absorbing zone for establishing and/or maintaining fluid transport through or along said at least one fluid passage is manufactured on the basis of a non-porous substrate, having projections substantially perpendicular to said surface, and said projections having a height, diameter and a distance or distances between the projections such, that lateral capillary flow of said fluid in said zone is achieved.

Abstract: The invention relates to a matrix suitable for use in the replication of a plastic element having a positive microstructure, comprising a first wear-resistant layer that is supported by a carrier element, wherein the matrix comprises a heating means for supplying electrical heat energy through the wear-resistant layer or carrier element. The invention further relates to a plastic element producing machine and a method for the manufacture of a plastic element having a surface with a positive microstructure.

Abstract: The invention relates to a micro fluidic system comprising a substrate, and, provided on said substrate, at least one flow path interconnecting with functional means in which liquid samples can be treated by desired procedures. The flow paths are laid out to form a pattern for the transport of liquid samples to and from said functional means. These flow paths comprise a plurality of micro posts protruding upwards from said substrate, the spacing between the micro posts being small enough to induce a capillary action in a liquid sample applied anywhere within any of said flow paths, so as to force said liquid to move from where said liquid sample was applied.

Abstract: A method of producing a plastic product having a microstructured surface (3a), utilizing an arrangement for moulding plastic products. Said arrangement has at least two mould parts (2, 3) movable towards and away from each other, a predetermined volume of a viscous polymerizable plastic material (9) being supplied to a cavity formed between the mould parts, after which polymerization of the plastic material occurs. After said polymerization of the plastic material (9′), the mould parts are caused to move apart from each other thereby allowing removal of a finished plastic product the outer shape of which conforms to the shape of the cavity produced by the mould parts. Said viscous polymerizable plastic material (9) is supplied to the cavity (6) around a hole (10a) pertaining to the finished plastic product, and said viscous plastic material is distributed in a direction from said hole (10a) towards the peripheral edge area of the plastic product.

Abstract: The present invention relates to a method for the manufacture of a matrix and to a matrix (1) thus manufactured, at least one surface section (2) displaying a microstructure, which matrix (3) is suitable for inclusion as a mould insert in a mould cavity or in a cavity, in a unit producing plastic components, in order to assign said plastic components an opposing micostructure in a corresponding surface section. An original (3) with a surface section (4) displaying a microstructure less than 500 &mgr;m is used in order to apply on this original layer upon layer of a material (11, 12, 13, 14) and/or mixtures of material producing a matrix, and thereafter the matrix (1) is removed from said original (3) or the material in the original is removed.

Abstract: The invention relates to a method for manufacturing a mould body (40) serving as an original or master or, alternatively, a matrix, said mould body having at least one surface area (41a) in the form of a microstructure, designed so that it can be transferred as a complementary surface area to a matrix or an object. The mould body (40) is constructed from one or more prefabricated discrete elements (41). A base plate belonging to the mould body (40) is applied and secured in at least one of these discrete elements (41). This element shall be provided with said surface area (41a) in the form of a microstructure. A matrix or object formed by the mould body (40) is provided with a complementary microstructured surface area from said discrete elements. The invention also relates to a mould body produced in this way.

Abstract: The invention relates to a method of producing a matrix suitable for use in a plastic element-producing machine, a matrix produced in accordance with the method and use of such a matrix to form a plastic element. One side of the matrix is provided with a negative microstructure which is replicated in plastic material to form a positive microstructure on a plastic element, wherewith the matrix can be produced by applying a layer of material to the positive microstructure side of a master. The positive microstructure side of the master has a thin wear-resistant layer which serves as a first wear surface, wherewith said layer will present irregularities which correspond essentially to the microstructure, and wherein at least said irregularities are filled-in with a plastic composite so as to form a carrier element for supporting the first wear layer. The layer can further be provided with means for supplying heat energy to said matrix.