Patents Assigned to Antibioticos S.p.A.
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Patent number: 8313922Abstract: The present invention relates to an improved process for the production of Daptomycin by fermentation with Streptomyces roseosporus, in the presence of n-decanal or Cuphea oil as sources of the n-decanoyl side chain. These reagents allow to reduce toxicity effects on the bacteria and to avoid the use of solvents in the feeding solution.Type: GrantFiled: July 30, 2009Date of Patent: November 20, 2012Assignee: Antibioticos S.p.A.Inventors: Gianluca Bertetti, Antonella Malcangi, Roberto Muraca, Guido Trione, Alessia Rossi
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Patent number: 8247426Abstract: Disclosed is a crystalline form of irinotecan hydrochloride (I) and processes for the preparation thereof from crude irinotecan hydrochloride or another polymorphic form of irinotecan. Said crystalline form is particularly suitable for industrial use, because it is easily filtered and possesses characteristics of high stability and purity.Type: GrantFiled: November 10, 2009Date of Patent: August 21, 2012Assignee: Antibioticos S.p.A.Inventors: Giovanni Pozzi, Paolo Ghetti, Gaetano Balsamo, Ettore Negri, Marco Alpegiani, Angelo Bedeschi, Roberta Pizzocaro
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Patent number: 7405294Abstract: Disclosed are salts of the general formula (I) wherein R1, R2 and B are defined in the description. These salts are useful intermediates for the preparation of cefdinir. Also, disclosed are processes for the preparation thereof from the compounds of the general formula (II) and (III). Further, disclosed is a method of producing cefdinir from the salts of the general formula (I) including the steps of removing protecting groups.Type: GrantFiled: September 26, 2003Date of Patent: July 29, 2008Assignee: Antibioticos S.p.A.Inventors: Giovanni Pozzi, Patricio Martin Gomez, Marco Alpegiani, Walter Cabri
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Patent number: 7399624Abstract: Enzymes with cephalosporin C acylase activity obtained by site-directed, random or “site-saturation” mutagenesis of a native sequence optimised for expression in E. coli.Type: GrantFiled: January 11, 2005Date of Patent: July 15, 2008Assignee: Antibioticos S.p.A.Inventors: Loredano Pollegioni, Mirella Pilone, Gianluca Molla, Eugenio Cucchetti, Roberto Verga, Walter Cabri
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Publication number: 20080160586Abstract: A process for the purification and recovery of Tacrolimus (I) (17-allyl-1,14-dihydroxy-12-[2-(4-hydroxy-3-methoxycyclohexyl)-1-methyvinyl]-23,25- dimethoxy-13,19,21,27-tetramethyl-11,28-dioxi-4-azatricyclo-[22.3.1.04.9]octacos-18-en-2,3,10,16-tetraone), starting from Streptomyces sp fermentation broth. The process is particularly advantageous in terms of productivity and selectivity of the separation of impurities.Type: ApplicationFiled: October 24, 2005Publication date: July 3, 2008Applicant: Antibioticos S.p.A.Inventors: Walter Cabri, Paolo Paissoni, Jacopo Roletto, Luca Morra
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Patent number: 7173126Abstract: Cefdinir crystalline salts of formula (I), in which n ranges from 1 to 3, the preparation and use thereof for the preparation and purification of cefdinir are herein disclosed. The salts of formula (I) can be obtained from cefdinir intermediates or crude cefdinir by treatment with phosphoric acid.Type: GrantFiled: December 1, 2003Date of Patent: February 6, 2007Assignee: Antibioticos S.p.A.Inventors: Giovanni Pozzi, Patricio Martin Gomez, Marco Alpegiani, Walter Cabri
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Publication number: 20060111566Abstract: Disclosed are salts of the general formula (I) wherein R1, R2 and as defined in the description and a process for the preparation thereof. These salts are useful intermediates for the preparation of cefdinir.Type: ApplicationFiled: September 26, 2003Publication date: May 25, 2006Applicant: Antibioticos S.p.A.Inventors: Giovanni Pozzi, Patricio Martinez Gomez, Marco Alpegiani, Walter Cabri
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Patent number: 7019140Abstract: A process for the synthesis of pergolide (Formula (I)) (D-6-n-propyl-8?methylmercaptomethylergo line) from acid 9,10-dihydrolysergic is herein disclosed. The process can be carried out without isolating most intermediates and is particularly convenient both from the yield and safety standpoint.Type: GrantFiled: March 10, 2003Date of Patent: March 28, 2006Assignee: Antibioticos S.p.A.Inventors: Walter Cabri, Paolo Paissoni, Jacopo Roletto, Piera Fonte, Sara Olmo
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Patent number: 6844455Abstract: The present invention discloses a process for the semi-synthesis of 4-demethoxydaunomycinone, (8s-cis)-acetyl-10-hydroxy-7,8,9,10-tetrahydro-6,8,11-trihydroxy-5,12-naphthacenedione, of formula (I).Type: GrantFiled: May 10, 2001Date of Patent: January 18, 2005Assignee: Antibioticos S.p.A.Inventors: Valentina Pizzamiglio, Walter Cabri, Elio Mapelli
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Patent number: 6642378Abstract: The invention is directed towards a process for the preparation of Cefuroxime acid or for a corresponding pharmaceutically acceptable salt or ester. The process comprises the carbamoylation of a Cefuroxime precursor with an activated isocyanate. Additionally, the process is characterized by the fact that a carbonic C1-C4 alkyl ester is used as a solvent for the carbamoylation reaction.Type: GrantFiled: March 13, 2002Date of Patent: November 4, 2003Assignee: Antibioticos S.p.A.Inventors: Walter Cabri, Enrico Siviero, Paola Luigia Daverio, Tania Cristiano, Claudio Felisi, Davide Longoni
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Publication number: 20030004336Abstract: A process for the preparation of Cefuroxime acid (1), which comprises the following steps: (1) Extraction of deacetyl 7-glutaryl ACA (II) aqueous solution at acid pH with organic solvents (for example according to the procedures disclosed in U.S. Pat. No. 5,801,241); (2) drying the resulting solution while preventing lactonization of the intermediate; (3) carbamoylation of the hydroxymethyl group at the 3-position by reaction with chlorosulfonyl isocyanate or similar products; (7) extraction of the carbamoyl derivative from step 3 with water at neutral pH; (8) enzymatic hydrolysis of the amide at the 7-position of the cephalosporanic ring with glutaryl acylase; (6) acylation of the amino group by condensation with 2-furanyl(sin-methoxyimino)acetic acid chloride or mixed anhydride.Type: ApplicationFiled: August 22, 2002Publication date: January 2, 2003Applicant: Antibioticos S.p.A.Inventors: Enrico Siviero, Walter Cabri, Daniele Mario Terrassan
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Patent number: 6458558Abstract: A process for the preparation of Cefuroxime acid (I), which comprises the following steps: (1) Extraction of deacetyl 7-glutaryl ACA (II) aqueous solution at acid pH with organic solvents (for example according to the procedures disclosed in U.S. Pat. No. 5,801,241); (2) drying the resulting solution while preventing lactonization of the intermediate; (3) carbamoylation of the hydroxymethyl group at the 3-position by reaction with chlorosulfonyl isocyanate or similar products; (7) extraction of the carbamoyl derivative from step 3 with water at neutral pH; (8) enzymatic hydrolysis of the amide at the 7-position of the cephalosporanic ring with glutaryl acylase; (6) acylation of the amnino group by condensation with 2-furanyl(sin-methoxyimino)acetic acid chloride or mixed anhydride.Type: GrantFiled: February 11, 2002Date of Patent: October 1, 2002Assignee: Antibioticos S.p.A.Inventors: Enrico Siviero, Walter Cabri, Daniele Mario Terrassan
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Patent number: 6005101Abstract: A compound having the following general formula (I): ##STR1## where R is a hydrogen atom; a linear or branched C.sub.1 -C.sub.4 alkyl group, unsubstituted or substituted by at least a phenyl group or at least a hydrogen atom; a benzyl group substituted by at least a linear or branched C.sub.1 -C.sub.4 alkyl or alkoxy group or a nitro group; a silyl substituted by at least a linear or branched, unsubstituted or substituted C.sub.1 -C.sub.4 alkyl group; n is 0 or 1; and Y is a radical of formula ##STR2## wherein A is H, OH, Cl, CH.sub.2, CH.sub.2 X, where X is F, Cl, Br, I, OH or OR' and R' is COCH.sub.3 or a linear or branched, unsubstituted or substituted C.sub.1 -C.sub.4 alkyl group and - - - represents a single or a double bond, with the proviso that when n=0 and R is H, R' is not a methyl group.Type: GrantFiled: February 3, 1998Date of Patent: December 21, 1999Assignee: Antibioticos S.p.A.Inventors: Loris Sogli, Davide Longoni, Giovanni Pozzi, Enrico Siviero, Daniele Mario Terrassan, Ermanno Bernasconi, Francisco Salto
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Patent number: 5945414Abstract: Cefazolin, cefazedone, cefoperazone, cefamandole, cefatrizine or ceftriaxone is prepared by reacting glutaryl 7-ACA of the formula: ##STR1## with a compound of formula (II):R--SH (II)wherein R is 5-methyl-1,3,4-thiadiazol-2-yl, 1H-1,2,3-triazol-4-yl, 1-methyl-tetrazol-5-yl or1,2,5,6-tetrahydro-2-methyl-5,6-dioxo-1,2,4-triazin-3-yl group, and R.sup.1 and R.sup.2 are both hydrogen and the other is an acyl group, in an aqueous solution in an amount of 3-5 mols per mol of glutaryl 7-ACA to about 90.degree. C. and for a time from about 2 to about 10 hours;optionally recovering the excess of the compound of formula (II), thereby preparing a compound of formula (III) in an aqueous solution: ##STR2## wherein R is as above defined and optionally deacylating said compound of formula (III); andconverting the resulting compound of formula (I) ##STR3## wherein R, R.sup.1 and R.sup.2 are as defined above in the presence of a non-chlorinated solvent into one of said cephalosporins.Type: GrantFiled: December 2, 1997Date of Patent: August 31, 1999Assignee: Antibioticos S.p.A.Inventors: Loris Sogli, Daniele Terrassan, Ermanno Bernasconi, Francisco Salto
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Patent number: 5750682Abstract: There are disclosed new compounds of formula (I), wherein R is: a hydrogen atom: a linear or branched C1-C4 alkyl group, unsubstituted or substituted by at least a phenyl group or at least a hydrogen atom: a benzyl group substituted by at least a linear or branched C1-C4 alkyl or alkoxy group or a nitro group; a silyl substituted by at least a linear or branched, unsubstituted or substituted C1-C4 alkyl group; n is 0 or 1; and Y is a radical of formula (II), wherein A is H, OH, Cl, CH2, CH2X, where X is F, Cl, Br, I, OH or OR' and R' is COCH3 or a linear or branched, unsubstituted or substituted C1-C4 alkyl group and . . . represents a single or a double bond, with the proviso that when n=0 and R is H, R' is not a methyl group; and processes for obtaining them.Type: GrantFiled: March 13, 1996Date of Patent: May 12, 1998Assignee: Antibioticos S.p.A.Inventors: Loris Sogli, Davide Longoni, Giovanni Pozzi, Enrico Siviero, Daniele Mario Terrassan, Ermanno Bernasconi, Francisco Salto
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Patent number: 5424196Abstract: Transformation of Cephalosporin C or its derivatives and salts into 7-aminocephalosporanic acid or its derivatives by an enzymatic two stage process with enzymes immobilized on a solid matrix.Type: GrantFiled: January 3, 1994Date of Patent: June 13, 1995Assignee: Antibioticos S.p.A.Inventors: Stefano Cambiaghi, Sergio Tomaselli, Roberto Verga
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Patent number: 5387679Abstract: The present invention relates to a process for preparing a compound of formula (I) ##STR1## wherein R is an heterocyclic group which contains at least one nitrogen atom with or without oxygen or sulphur and R.sup.1 and R.sup.2 are both hydrogen atoms or one of them is an hydrogen atom and the other is an acyl group; the process comprising reacting a compound of formula (II) ##STR2## wherein R.sup.1 and R.sup.2 are each as defined above, and wherein, if necessary, any reactive group is protected by a suitable protective group, or a salt thereof, with a compound of formula (III)R--SH (III)wherein R is as defined above, or a salt thereof, in the presence of an acid and of a compound of formula (IV) ##STR3## wherein each of R.sup.3 and R.sup.4 is a C.sub.1 -C.sub.4 alkyl group or R.sup.3 and R.sup.4 taken together are a C.sub.2 or C.sub.3 alkylene chain and, if necessary, removing the protective groups possibly present.Type: GrantFiled: March 15, 1993Date of Patent: February 7, 1995Assignee: Antibioticos S.p.A.Inventors: Loris Sogli, Daniele Terrassan, Giuseppe Ribaldone
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Patent number: 5354667Abstract: A process for the production of an E. coli strain producing high yields of 7.beta.-(4-carboxybutanamido) cephalosporin acylase, comprises: (a) digesting the DNA of a microorganism whose DNA includes the sequence encoding 7.beta.-(4-carboxybutanamido) cephalosporin acylase, and forming a plasmidic library; (b) transforming, with the sequences in the plasmidic library, an auxotrophic E. coli host; (c) selecting for transformed E. coli hosts containing the acylase sequence by growth on a suitable medium; (d) isolating the vector containing the acylase sequence, digesting the vector, and ligating the DNA sequences obtained into an E. coli vector under control of an E. coli promoter; (e) repeating selection procedure of step (c) ; (f) using the vectors from the selected E. coli hosts to transform an E. coli host lacking substantial .beta.-lactamase activity. 7-Aminocephalosporanic acid and its derivatives can be prepared by reaction of substrates like 7.beta.-(4-carboxybutanamido) cephalosporanic acid with 7.beta.Type: GrantFiled: August 5, 1991Date of Patent: October 11, 1994Assignee: Antibioticos, S.p.A.Inventors: Christian Croux, Javier C. Perez, Jose L. B. Fuentes, Francisco S. Maldonado