Patents Assigned to Antibioticos S.p.A.
  • Patent number: 8313922
    Abstract: The present invention relates to an improved process for the production of Daptomycin by fermentation with Streptomyces roseosporus, in the presence of n-decanal or Cuphea oil as sources of the n-decanoyl side chain. These reagents allow to reduce toxicity effects on the bacteria and to avoid the use of solvents in the feeding solution.
    Type: Grant
    Filed: July 30, 2009
    Date of Patent: November 20, 2012
    Assignee: Antibioticos S.p.A.
    Inventors: Gianluca Bertetti, Antonella Malcangi, Roberto Muraca, Guido Trione, Alessia Rossi
  • Patent number: 8247426
    Abstract: Disclosed is a crystalline form of irinotecan hydrochloride (I) and processes for the preparation thereof from crude irinotecan hydrochloride or another polymorphic form of irinotecan. Said crystalline form is particularly suitable for industrial use, because it is easily filtered and possesses characteristics of high stability and purity.
    Type: Grant
    Filed: November 10, 2009
    Date of Patent: August 21, 2012
    Assignee: Antibioticos S.p.A.
    Inventors: Giovanni Pozzi, Paolo Ghetti, Gaetano Balsamo, Ettore Negri, Marco Alpegiani, Angelo Bedeschi, Roberta Pizzocaro
  • Patent number: 7405294
    Abstract: Disclosed are salts of the general formula (I) wherein R1, R2 and B are defined in the description. These salts are useful intermediates for the preparation of cefdinir. Also, disclosed are processes for the preparation thereof from the compounds of the general formula (II) and (III). Further, disclosed is a method of producing cefdinir from the salts of the general formula (I) including the steps of removing protecting groups.
    Type: Grant
    Filed: September 26, 2003
    Date of Patent: July 29, 2008
    Assignee: Antibioticos S.p.A.
    Inventors: Giovanni Pozzi, Patricio Martin Gomez, Marco Alpegiani, Walter Cabri
  • Patent number: 7399624
    Abstract: Enzymes with cephalosporin C acylase activity obtained by site-directed, random or “site-saturation” mutagenesis of a native sequence optimised for expression in E. coli.
    Type: Grant
    Filed: January 11, 2005
    Date of Patent: July 15, 2008
    Assignee: Antibioticos S.p.A.
    Inventors: Loredano Pollegioni, Mirella Pilone, Gianluca Molla, Eugenio Cucchetti, Roberto Verga, Walter Cabri
  • Publication number: 20080160586
    Abstract: A process for the purification and recovery of Tacrolimus (I) (17-allyl-1,14-dihydroxy-12-[2-(4-hydroxy-3-methoxycyclohexyl)-1-methyvinyl]-23,25- dimethoxy-13,19,21,27-tetramethyl-11,28-dioxi-4-azatricyclo-[22.3.1.04.9]octacos-18-en-2,3,10,16-tetraone), starting from Streptomyces sp fermentation broth. The process is particularly advantageous in terms of productivity and selectivity of the separation of impurities.
    Type: Application
    Filed: October 24, 2005
    Publication date: July 3, 2008
    Applicant: Antibioticos S.p.A.
    Inventors: Walter Cabri, Paolo Paissoni, Jacopo Roletto, Luca Morra
  • Patent number: 7173126
    Abstract: Cefdinir crystalline salts of formula (I), in which n ranges from 1 to 3, the preparation and use thereof for the preparation and purification of cefdinir are herein disclosed. The salts of formula (I) can be obtained from cefdinir intermediates or crude cefdinir by treatment with phosphoric acid.
    Type: Grant
    Filed: December 1, 2003
    Date of Patent: February 6, 2007
    Assignee: Antibioticos S.p.A.
    Inventors: Giovanni Pozzi, Patricio Martin Gomez, Marco Alpegiani, Walter Cabri
  • Publication number: 20060111566
    Abstract: Disclosed are salts of the general formula (I) wherein R1, R2 and as defined in the description and a process for the preparation thereof. These salts are useful intermediates for the preparation of cefdinir.
    Type: Application
    Filed: September 26, 2003
    Publication date: May 25, 2006
    Applicant: Antibioticos S.p.A.
    Inventors: Giovanni Pozzi, Patricio Martinez Gomez, Marco Alpegiani, Walter Cabri
  • Patent number: 7019140
    Abstract: A process for the synthesis of pergolide (Formula (I)) (D-6-n-propyl-8?methylmercaptomethylergo line) from acid 9,10-dihydrolysergic is herein disclosed. The process can be carried out without isolating most intermediates and is particularly convenient both from the yield and safety standpoint.
    Type: Grant
    Filed: March 10, 2003
    Date of Patent: March 28, 2006
    Assignee: Antibioticos S.p.A.
    Inventors: Walter Cabri, Paolo Paissoni, Jacopo Roletto, Piera Fonte, Sara Olmo
  • Patent number: 6844455
    Abstract: The present invention discloses a process for the semi-synthesis of 4-demethoxydaunomycinone, (8s-cis)-acetyl-10-hydroxy-7,8,9,10-tetrahydro-6,8,11-trihydroxy-5,12-naphthacenedione, of formula (I).
    Type: Grant
    Filed: May 10, 2001
    Date of Patent: January 18, 2005
    Assignee: Antibioticos S.p.A.
    Inventors: Valentina Pizzamiglio, Walter Cabri, Elio Mapelli
  • Patent number: 6642378
    Abstract: The invention is directed towards a process for the preparation of Cefuroxime acid or for a corresponding pharmaceutically acceptable salt or ester. The process comprises the carbamoylation of a Cefuroxime precursor with an activated isocyanate. Additionally, the process is characterized by the fact that a carbonic C1-C4 alkyl ester is used as a solvent for the carbamoylation reaction.
    Type: Grant
    Filed: March 13, 2002
    Date of Patent: November 4, 2003
    Assignee: Antibioticos S.p.A.
    Inventors: Walter Cabri, Enrico Siviero, Paola Luigia Daverio, Tania Cristiano, Claudio Felisi, Davide Longoni
  • Publication number: 20030004336
    Abstract: A process for the preparation of Cefuroxime acid (1), which comprises the following steps: (1) Extraction of deacetyl 7-glutaryl ACA (II) aqueous solution at acid pH with organic solvents (for example according to the procedures disclosed in U.S. Pat. No. 5,801,241); (2) drying the resulting solution while preventing lactonization of the intermediate; (3) carbamoylation of the hydroxymethyl group at the 3-position by reaction with chlorosulfonyl isocyanate or similar products; (7) extraction of the carbamoyl derivative from step 3 with water at neutral pH; (8) enzymatic hydrolysis of the amide at the 7-position of the cephalosporanic ring with glutaryl acylase; (6) acylation of the amino group by condensation with 2-furanyl(sin-methoxyimino)acetic acid chloride or mixed anhydride.
    Type: Application
    Filed: August 22, 2002
    Publication date: January 2, 2003
    Applicant: Antibioticos S.p.A.
    Inventors: Enrico Siviero, Walter Cabri, Daniele Mario Terrassan
  • Patent number: 6458558
    Abstract: A process for the preparation of Cefuroxime acid (I), which comprises the following steps: (1) Extraction of deacetyl 7-glutaryl ACA (II) aqueous solution at acid pH with organic solvents (for example according to the procedures disclosed in U.S. Pat. No. 5,801,241); (2) drying the resulting solution while preventing lactonization of the intermediate; (3) carbamoylation of the hydroxymethyl group at the 3-position by reaction with chlorosulfonyl isocyanate or similar products; (7) extraction of the carbamoyl derivative from step 3 with water at neutral pH; (8) enzymatic hydrolysis of the amide at the 7-position of the cephalosporanic ring with glutaryl acylase; (6) acylation of the amnino group by condensation with 2-furanyl(sin-methoxyimino)acetic acid chloride or mixed anhydride.
    Type: Grant
    Filed: February 11, 2002
    Date of Patent: October 1, 2002
    Assignee: Antibioticos S.p.A.
    Inventors: Enrico Siviero, Walter Cabri, Daniele Mario Terrassan
  • Patent number: 6005101
    Abstract: A compound having the following general formula (I): ##STR1## where R is a hydrogen atom; a linear or branched C.sub.1 -C.sub.4 alkyl group, unsubstituted or substituted by at least a phenyl group or at least a hydrogen atom; a benzyl group substituted by at least a linear or branched C.sub.1 -C.sub.4 alkyl or alkoxy group or a nitro group; a silyl substituted by at least a linear or branched, unsubstituted or substituted C.sub.1 -C.sub.4 alkyl group; n is 0 or 1; and Y is a radical of formula ##STR2## wherein A is H, OH, Cl, CH.sub.2, CH.sub.2 X, where X is F, Cl, Br, I, OH or OR' and R' is COCH.sub.3 or a linear or branched, unsubstituted or substituted C.sub.1 -C.sub.4 alkyl group and - - - represents a single or a double bond, with the proviso that when n=0 and R is H, R' is not a methyl group.
    Type: Grant
    Filed: February 3, 1998
    Date of Patent: December 21, 1999
    Assignee: Antibioticos S.p.A.
    Inventors: Loris Sogli, Davide Longoni, Giovanni Pozzi, Enrico Siviero, Daniele Mario Terrassan, Ermanno Bernasconi, Francisco Salto
  • Patent number: 5945414
    Abstract: Cefazolin, cefazedone, cefoperazone, cefamandole, cefatrizine or ceftriaxone is prepared by reacting glutaryl 7-ACA of the formula: ##STR1## with a compound of formula (II):R--SH (II)wherein R is 5-methyl-1,3,4-thiadiazol-2-yl, 1H-1,2,3-triazol-4-yl, 1-methyl-tetrazol-5-yl or1,2,5,6-tetrahydro-2-methyl-5,6-dioxo-1,2,4-triazin-3-yl group, and R.sup.1 and R.sup.2 are both hydrogen and the other is an acyl group, in an aqueous solution in an amount of 3-5 mols per mol of glutaryl 7-ACA to about 90.degree. C. and for a time from about 2 to about 10 hours;optionally recovering the excess of the compound of formula (II), thereby preparing a compound of formula (III) in an aqueous solution: ##STR2## wherein R is as above defined and optionally deacylating said compound of formula (III); andconverting the resulting compound of formula (I) ##STR3## wherein R, R.sup.1 and R.sup.2 are as defined above in the presence of a non-chlorinated solvent into one of said cephalosporins.
    Type: Grant
    Filed: December 2, 1997
    Date of Patent: August 31, 1999
    Assignee: Antibioticos S.p.A.
    Inventors: Loris Sogli, Daniele Terrassan, Ermanno Bernasconi, Francisco Salto
  • Patent number: 5750682
    Abstract: There are disclosed new compounds of formula (I), wherein R is: a hydrogen atom: a linear or branched C1-C4 alkyl group, unsubstituted or substituted by at least a phenyl group or at least a hydrogen atom: a benzyl group substituted by at least a linear or branched C1-C4 alkyl or alkoxy group or a nitro group; a silyl substituted by at least a linear or branched, unsubstituted or substituted C1-C4 alkyl group; n is 0 or 1; and Y is a radical of formula (II), wherein A is H, OH, Cl, CH2, CH2X, where X is F, Cl, Br, I, OH or OR' and R' is COCH3 or a linear or branched, unsubstituted or substituted C1-C4 alkyl group and . . . represents a single or a double bond, with the proviso that when n=0 and R is H, R' is not a methyl group; and processes for obtaining them.
    Type: Grant
    Filed: March 13, 1996
    Date of Patent: May 12, 1998
    Assignee: Antibioticos S.p.A.
    Inventors: Loris Sogli, Davide Longoni, Giovanni Pozzi, Enrico Siviero, Daniele Mario Terrassan, Ermanno Bernasconi, Francisco Salto
  • Patent number: 5424196
    Abstract: Transformation of Cephalosporin C or its derivatives and salts into 7-aminocephalosporanic acid or its derivatives by an enzymatic two stage process with enzymes immobilized on a solid matrix.
    Type: Grant
    Filed: January 3, 1994
    Date of Patent: June 13, 1995
    Assignee: Antibioticos S.p.A.
    Inventors: Stefano Cambiaghi, Sergio Tomaselli, Roberto Verga
  • Patent number: 5387679
    Abstract: The present invention relates to a process for preparing a compound of formula (I) ##STR1## wherein R is an heterocyclic group which contains at least one nitrogen atom with or without oxygen or sulphur and R.sup.1 and R.sup.2 are both hydrogen atoms or one of them is an hydrogen atom and the other is an acyl group; the process comprising reacting a compound of formula (II) ##STR2## wherein R.sup.1 and R.sup.2 are each as defined above, and wherein, if necessary, any reactive group is protected by a suitable protective group, or a salt thereof, with a compound of formula (III)R--SH (III)wherein R is as defined above, or a salt thereof, in the presence of an acid and of a compound of formula (IV) ##STR3## wherein each of R.sup.3 and R.sup.4 is a C.sub.1 -C.sub.4 alkyl group or R.sup.3 and R.sup.4 taken together are a C.sub.2 or C.sub.3 alkylene chain and, if necessary, removing the protective groups possibly present.
    Type: Grant
    Filed: March 15, 1993
    Date of Patent: February 7, 1995
    Assignee: Antibioticos S.p.A.
    Inventors: Loris Sogli, Daniele Terrassan, Giuseppe Ribaldone
  • Patent number: 5354667
    Abstract: A process for the production of an E. coli strain producing high yields of 7.beta.-(4-carboxybutanamido) cephalosporin acylase, comprises: (a) digesting the DNA of a microorganism whose DNA includes the sequence encoding 7.beta.-(4-carboxybutanamido) cephalosporin acylase, and forming a plasmidic library; (b) transforming, with the sequences in the plasmidic library, an auxotrophic E. coli host; (c) selecting for transformed E. coli hosts containing the acylase sequence by growth on a suitable medium; (d) isolating the vector containing the acylase sequence, digesting the vector, and ligating the DNA sequences obtained into an E. coli vector under control of an E. coli promoter; (e) repeating selection procedure of step (c) ; (f) using the vectors from the selected E. coli hosts to transform an E. coli host lacking substantial .beta.-lactamase activity. 7-Aminocephalosporanic acid and its derivatives can be prepared by reaction of substrates like 7.beta.-(4-carboxybutanamido) cephalosporanic acid with 7.beta.
    Type: Grant
    Filed: August 5, 1991
    Date of Patent: October 11, 1994
    Assignee: Antibioticos, S.p.A.
    Inventors: Christian Croux, Javier C. Perez, Jose L. B. Fuentes, Francisco S. Maldonado