Patents Assigned to Applied Biosystems
  • HIGH POTENCY SIRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES
    Publication number: 20140099715
    Abstract: The present invention provides improved methods of attenuating gene expression through the phenomenon of RNA interference. The invention provides methods of synthesis of double stranded RNAs (dsRNAs) of increased potency for use as small interfering RNA (siRNA). Surprisingly and unexpectedly, siRNAs made by the methods of the invention are significantly more potent than previously available siRNAs.
    Type: Application
    Filed: August 21, 2013
    Publication date: April 10, 2014
    Applicant: Applied Biosystems, LLC
    Inventors: David Brown, Lance P. Ford, Vince Pallotta, Brittan L. Pasloske, Richard A. Jarvis
  • High Speed, High Resolution Compositions, Methods and Kits for Capillary Electrophoresis
    Publication number: 20140090980
    Abstract: The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.
    Type: Application
    Filed: August 23, 2013
    Publication date: April 3, 2014
    Applicant: Applied Biosystems, LLC
    Inventors: Karl O. VOSS, Aldrich N.K. LAU
  • SiRNA Sequence-Independent Modification Formats for Reducing Off-Target Phenotypic Effects in RNAi, and Stabilized Forms Thereof
    Publication number: 20140094501
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Application
    Filed: August 23, 2013
    Publication date: April 3, 2014
    Applicant: Applied Biosystems, LLC
    Inventors: Nitin PURI, Irudaya CHARLES, Susan MAGDALENO, Alexander VLASSOV, Christopher BURNETT
  • Axial Illumination for Capillary Electrophoresis
    Publication number: 20140091233
    Abstract: System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.
    Type: Application
    Filed: September 20, 2013
    Publication date: April 3, 2014
    Applicant: Applied Biosystems, LLC
    Inventors: Richard REEL, Eric Nordman
  • Thermal cycler for PCR
    Patent number: 8685717
    Abstract: An apparatus comprising an assembly and a cover. The assembly comprises a sample block configured to receive a microtiter tray having a plurality of vials, each vial comprising a cap having a surface and a perimeter. The cover comprises a platen, vertically and horizontally displaceable in relationship to the sample block, the platen having a plurality of recesses. Each recess corresponds to a respective vial and is shaped and arranged to clear the surface of its respective cap when the cover is displaced onto the sample block. The cover includes a skirt in contact with and surrounding a perimeter of the platen. The skirt is configured for mating with a perimeter of a microtiter tray when a microtiter tray is received in the sample block, and also configured such that the cover contacts a microtiter tray when a microtiter tray is received in the sample block.
    Type: Grant
    Filed: January 17, 2007
    Date of Patent: April 1, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: John G. Atwood, Adrian Fawcett, Keith S. Ferrara, Paul M. Hetherington, Richard W. Noreiks, Douglas E. Olsen, John R. Widomski, Charles M. Wittmer
  • Device for carrying out chemical or biological reactions
    Patent number: 8676383
    Abstract: The invention relates to a device for carrying out of chemical or biological reactions with a reaction vessel receiving element for receiving a microtiter plate with several reaction vessels, wherein the reaction vessel receiving element has several recesses arranged in a regular pattern to receive the respective reaction vessels, a heating device for heating the reaction vessel receiving element, and a cooling device for cooling the reaction vessel. The invention is characterized by the fact that the reaction vessel receiving element is divided into several segments. The individual segments are thermally decoupled from one another, and each segment is assigned a heating device which may be actuated independently of the others. By means of the segmentation of the reaction vessel receiving element, it is possible for zones to be set and held at different temperatures.
    Type: Grant
    Filed: September 5, 2007
    Date of Patent: March 18, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Lim Hi Tan, Jew Kwee Ngui, Hon Siu Shin, Ui Leng Soh, Yang Hooi Kee, Hock Lai Khoo, Mark T. Reed, Ching Ong Lau, Wolfgang Heimberg
  • Methods and systems for using RFID in biological field
    Patent number: 8669849
    Abstract: Biological reagent carrier devices and methods are disclosed, which employ RFID techniques to associate information with biological reagents.
    Type: Grant
    Filed: November 29, 2012
    Date of Patent: March 11, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Richard D. Morris, Jerome A. Mack
  • Methods and systems for using RFID in biological field
    Patent number: 8669848
    Abstract: Biological reagent carrier devices and methods are disclosed, which employ RFID techniques to associate information with biological reagents.
    Type: Grant
    Filed: September 13, 2012
    Date of Patent: March 11, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Richard D. Morris, Jerome A. Mack
  • Methods and systems for using RFID in biological field
    Patent number: 8665071
    Abstract: Biological reagent carrier devices and methods are disclosed, which employ RFID techniques to associate information with biological reagents.
    Type: Grant
    Filed: November 29, 2012
    Date of Patent: March 4, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Richard D. Morris, Jerome A. Mack
  • Devices and methods for the detection of analytes
    Patent number: 8658093
    Abstract: System and methods for detecting analytes such as pathogenic cells are described. The methods allow for the direct measurement of analytes such as pathogenic organisms without the need for sample preparation and/or PCR. The devices can be used individually as point-of-use sensors for airborne pathogens and other pathogenic organisms in foods and agriculture products.
    Type: Grant
    Filed: July 3, 2008
    Date of Patent: February 25, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Aldrich N. K. Lau, Robert G. Eason, Kristian M. Scaboo, Handong Li
  • Luminescence reference standards
    Patent number: 8659755
    Abstract: The present teachings provide for systems, and components thereof, for detecting and/or analyzing light. These systems can include, among others, optical reference standards utilizing luminophores, such as nanocrystals, for calibrating, validating, and/or monitoring light-detection systems, before, during, and/or after sample analysis.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: February 25, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: J. Michael Phillips, Kevin S. Bodner, Aldrich N. K. Lau, Steven J. Boege, Mark F. Oldham, Donald R. Sandell, David H. Tracy
  • Automatic threshold setting and baseline determination for real-time PCR
    Patent number: 8649982
    Abstract: The invention discloses a system and methods for quantitating the presence of nucleic acid sequences by evaluation of amplification data generated using real-time PCR. In one aspect, the methods may be adapted to identify a threshold and threshold cycle for one or more reactions based upon evaluation of exponential and baseline regions for each amplification reaction. The methodology used in the analysis may be readily automated such that subjective user interpretation of the data is substantially reduced or eliminated.
    Type: Grant
    Filed: September 13, 2011
    Date of Patent: February 11, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: David Woo, Clinton Lewis, Nasser Abbasi
  • Fluid processing device for oligonucleotide synthesis and analysis
    Patent number: 8642354
    Abstract: The present teachings provide a fluid processing device adapted to produce different oligomers in a plurality of respective reaction sites. The fluid processing device can comprise a first manifold for delivering reactants to the plurality of reaction sites, and a second manifold for removing waste from, and optionally delivering wash fluid to, the plurality of reaction sites. Surface tension control valves can be disposed in fluid communication with the first manifold and can selectively allow reactants and/or fluids into the reaction sites. A method of making oligonucleotides is also provided.
    Type: Grant
    Filed: November 11, 2010
    Date of Patent: February 4, 2014
    Assignee: Applied Biosystems, LLC
    Inventor: Sergey Ermakov
  • Sample chamber array and method for processing a biological sample
    Patent number: 8641987
    Abstract: A sample chamber array is provided. The sample chamber array may comprise at least one reservoir in fluid communication with at least one sample chamber, and a movable portion defining the sample chamber. The reservoir is fillable with a liquid biological sample. The movable portion may be movable with respect to the remainder of the sample chamber from a first position to a second position. In the first position the movable portion is concave and the sample chamber is without biological sample. In the second position the movable portion is convex and the sample chamber comprises biological sample. The movement of the movable portion to the second position causes a pressure drop to transport the biological sample into the sample chamber from the at least one reservoir. Methods for processing a biological sample and methods of making a sample chamber array are also provided.
    Type: Grant
    Filed: January 24, 2003
    Date of Patent: February 4, 2014
    Assignee: Applied Biosystems, LLC
    Inventor: Donald R. Sandell
  • Optical camera alignment
    Patent number: 8638509
    Abstract: A camera alignment system that can enable alignment in at least one of three planes and about an axis of at least one of the planes. An alignment mount can mate to a camera and lens. The alignment mount can comprise a mechanism to adjust the camera relative to the lens to that an image plane of the camera aligns with an image plane of the lens in a predetermined orientation. One predetermined orientation can be that the image plane of the camera being parallel to the image plane of the lens.
    Type: Grant
    Filed: October 3, 2011
    Date of Patent: January 28, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Barry Blasenheim, H. Pin Kao, Mark Oldham
  • Non-fluorescent quencher compounds and biomolecular assays
    Patent number: 8637658
    Abstract: Bis-diazo, triaryl and aryldiazo-N-arylphenazonium quencher moieties, substituted with electron-withdrawing and electron-donating substituents which induce polarity in the delocalized aryl/diazo ring systems, are useful as labels when attached to biomolecules such as polynucleotides, nucleosides, nucleotides and polypeptides. The quencher moieties are non-fluorescent and accept energy transfer from fluorescent reporter labels by any energy-transfer mechanism, such as FRET. Fluorescence quencher compositions are useful in preparing quencher labeled biomolecules for various molecular biology assays based on fluorescence detection.
    Type: Grant
    Filed: September 27, 2012
    Date of Patent: January 28, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Gregory J. Ewing, Khairuzzaman Bashar Mullah, Ronald Graham
  • Methods of detecting target nucleic acids
    Patent number: 8637247
    Abstract: The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore.
    Type: Grant
    Filed: August 21, 2012
    Date of Patent: January 28, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Muhammad Sharaf, Timothy Woudenberg, Khairuzzaman Bashar Mullah
  • Linear amplification of short nucleic acids
    Patent number: 8628925
    Abstract: The present teachings provide novel methods for amplifying short nucleic acids. In some embodiments, the present teachings provide novel methods for linearly amplifying a collection of micro RNAs by using temperature cycling during a reverse transcription reaction. The cycling can comprise at least 20 cycles of an annealing temperature segment of 10° C.-30° C., and a denaturation temperature segment of 35° C.-60° C. In some embodiments, the temperature cycled reaction can comprise an osmolyte.
    Type: Grant
    Filed: June 6, 2011
    Date of Patent: January 14, 2014
    Assignee: Applied Biosystems, LLC
    Inventor: Will Bloch
  • Sample substrate having a divided sample chamber and method of loading thereof
    Patent number: 8628730
    Abstract: A sample substrate configured for samples of biological material is provided. The sample substrate has a dual chambered sample well separated by a wall that may be punctured or otherwise breached to allow mixing of material contained in the two initially separate chambers. The chambers are connected by channels to fluid reservoirs, wherein the channels can be staked to prevent further fluid flow into and out of the chambers. Methods of loading a sample substrate are also provided.
    Type: Grant
    Filed: February 15, 2008
    Date of Patent: January 14, 2014
    Assignee: Applied Biosystems, LLC
    Inventor: Donald R. Sandell
  • Methods and kits using extended rhodamine dyes
    Patent number: 8628975
    Abstract: Extended rhodamine compounds exhibiting favorable fluorescence characteristics having the structure are disclosed. In addition, novel intermediates for synthesis of these dyes are disclosed, such intermediates having the structure In addition, methods of making and using the dyes as fluorescent labels are disclosed.
    Type: Grant
    Filed: February 3, 2012
    Date of Patent: January 14, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Joe Y. L. Lam, Scott C. Benson, Steven M. Menchen