Abstract: Provided herein are methods of detecting unlabeled biomolecules. In some embodiments, the methods include disrupting a cell population sufficient to release unlabeled biomolecules from the cell population to produce released biomolecules in which the cell population is disposed on a first inner surface of a chamber that is disposed substantially within a fluidic device and in which the chamber comprises a fluidic material. In some embodiments, the methods also include binding the released biomolecules to a second inner surface of the chamber to produce surface-bound biomolecules, introducing an incident light toward the second inner surface of the chamber concurrent with, and/or after, producing the surface-bound biomolecules, and detecting light scattered by the surface-bound biomolecules over a duration to produce a set of biomolecule imaging data. Related fluidic devices, systems, and computer readable media are also provided.

Abstract: A system and method for computing in memory with artificial neurons. According to an embodiment of the present disclosure, there is provided a system, including: a computer-readable memory; a neuron processing element communicatively connected to the computer-readable memory, the neuron processing element including: a plurality of configurable processing circuits each having a plurality of outputs and a plurality of inputs; and a network connecting one or more of the outputs of the configurable processing circuits to one or more of the inputs of the configurable processing circuits, each of the configurable processing circuits including: an artificial neuron having a plurality of inputs; and a register connected to the inputs of the artificial neuron.

Abstract: An additive manufacturing process for forming a metallic layer on the surface of the substrate includes fabricating a substrate from a polymerizable composition by a stereolithographic process, and contacting the reactive surface with an aqueous solution including a metal precursor. The metal precursor includes a metal, and the polymerizable composition includes a multiplicity of multifunctional components. Each multifunctional component includes a reactive moiety extending from a surface of the substrate to form a reactive surface. An interface between the reactive surface and the aqueous solution is selectively irradiated to form nanoparticles including the metal in a desired pattern. The nanoparticles are chemically coupled to the reactive surface by reactive moieties, thereby forming a metallic layer on the surface of the substrate.

Abstract: Various embodiments of an amphibious submersible vehicle for use in non-destructive testing of pipe interiors and walls are disclosed herein. In one aspect, the vehicle is operable for amphibious submersible operation such that pipes of various diameters can be inspected under full, partially full, and dry conditions. In another aspect, the vehicle is equipped with a plurality of propellers for travel when fully or partially submerged in water and a plurality of wheels for traveling when in contact with a pipe wall or for traveling over debris. In some embodiments, the vehicle is equipped with a plurality of sensors configured for imaging and navigation which enable the vehicle for pipe inspection and identification of problem areas.

Abstract: Described herein are biosensor microarrays comprising detector polypeptide monolayers substantially free of contaminants. Also provided are methods for generation of such biosensor microarrays by capture of polypeptides by arrays comprising capture moieties and associated sensors.

Abstract: Treating plastic particles for use in concrete includes combining plastic particles with oil to yield a mixture, heating the mixture to yield a heated mixture, cooling the heated mixture to yield a cooled mixture, and removing excess oil from the cooled mixture to yield oil-treated plastic particles (e.g., oil-treated plastic particles for concrete). In one example, the oil is vegetable oil. The vegetable oil can be soybean oil, corn oil, canola oil, safflower oil, peanut oil, olive oil, grape seed oil, cocoa butter, palm oil, rice bran oil, or a combination thereof. The oil can be waste oil (e.g., waste vegetable oil, such as that recovered from restaurants). The plastic particles can be derived from post-consumer plastic, such as recycled plastic. In one example, the post-consumer plastic includes mixed plastics. A concrete composition can include rocks, sand, cement, and the oil-treated plastic particles.

Abstract: Provided herein are methods of detecting Ebola virus in a sample. The methods include contacting the sample with a plurality of gold nanoparticles (AuNPs) that are conjugated with antibodies, or antigen binding portions thereof, that bind to an epitope of a secreted glycoprotein (sGP) from the Ebola virus under conditions sufficient for the antibodies, or the antigen binding portions thereof, to bind to the epitope of the sGP from the Ebola virus in the sample to produce bound sGP. The methods also include detecting the sGP from the Ebola virus when aggregations of the bound sGP form with one another. Related reaction mixtures, devices, kits, and systems are also provided.

Abstract: The present disclosure relates to methods of culturing algae that overcomes catabolic repression of photosynthesis in mixotrophic growth.

Abstract: Provided herein are methods and systems for sensitive and multiplexed in situ analysis of samples such as biological samples using cleavable hapten linked targeting agents and cleavable detectably-labeled hapten-binding agents. In particular, provided herein are methods for multiplexed single-cell in situ biomolecule profiling in samples, including fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

Abstract: Described herein are polyhedral, three-dimensional tunable nanocages assembled with a multimeric protein covalently linked to a polynucleotide handle and a DNA origami base assembly including sequences complementary to the polynucleotide handles, wherein the polynucleotide handle and the complementary sequences hybridize to for double-stranded DNA helices.

Abstract: A fabric-based, soft-bodied aerial robot includes contact-reactive perching and embodied impact protection structures while remaining lightweight and streamlined. The aerial robot is operable to 1) pneumatically vary its body stiffness for collision resilience and 2) utilize a hybrid fabric-based, bistable (HFB) grasper to perform passive grasping. When compared to conventional rigid drone frames the soft-bodied aerial robot successfully demonstrates its ability to dissipate impact from head-on collisions and maintain flight stability without any structural damage. Furthermore, in dynamic perching scenarios the HFB grasper is capable to convert impact energy upon contact into firm grasp through rapid body shape conforming in less than 4 ms.

Abstract: Disclosed herein are compositions comprising a polypeptide with at least two domains, wherein the first domain is capable of binding CD3 and the second domain is capable of binding to a cancer cell. Also disclosed herein are methods of treating cancer in a subject, comprising: providing a composition comprising a polypeptide with at least two domains, wherein the first domain is capable of binding CD3 and the second domain is capable of binding to a cancer cell; and treating the cancer by administering a therapeutically effective dosage of the composition to the subject.

Abstract: Forming a lithium lanthanum zirconate thin film includes disposing zirconium oxide on a substrate to yield a zirconium oxide coating, contacting the zirconium oxide coating with a solution including a lithium salt and a lanthanum salt, heating the substrate to yield a dried salt coating on the zirconium oxide coating, melting the dried salt coating to yield a molten salt mixture, reacting the molten salt mixture with the zirconium oxide coating to yield lithium lanthanum zirconate, and cooling the lithium lanthanum zirconate to yield a lithium lanthanum zirconate coating on the substrate. In some cases, the zirconium oxide coating is contacted with an aqueous molten salt mixture including a lithium salt and a lanthanum salt, the molten salt mixture is reacted with the zirconium oxide coating to yield lithium lanthanum zirconate, and the lithium lanthanum zirconate is cooled to yield a lithium lanthanum zirconate coating on the substrate.

Abstract: A hyper-precise positioning and communications (HPPC) system and network are provided. The HPPC system is a next-generation positioning technology that promises a low-cost, high-performance solution to the need for more sophisticated positioning technologies in increasingly cluttered environments. The HPPC system is a joint positioning-communications radio technology that simultaneously performs relative positioning and secure communications. Both of these tasks are performed with a single, co-use waveform, which efficiently utilizes limited resources and supports higher user densities. Aspects of this disclosure include an HPPC system for a network which includes an arbitrary number of network nodes (e.g., radio frequency (RF) devices communicating over a joint positions-communications waveform). As such, networking protocols and design of data link and physical layers are described herein.

Abstract: The present technology relates to improved device and methods of use of insulator-based dielectrophoresis. This device provides a multi-length scale element that provides enhanced resolution and separation. The device provides improved particle streamlines, trapping efficiency, and induces laterally similar environments. Also provided are methods of using the device.

Abstract: Large intelligent surfaces (LISs) with sparse channel sensors are provided. Embodiments described herein provide efficient solutions for these problems by leveraging tools from compressive sensing and deep learning. Consequently, an LIS architecture based on sparse channel sensors is provided where all LIS elements are passive reconfigurable elements except for a few elements that are active (e.g., connected to baseband). Two solutions are developed that design LIS reflection matrices with negligible training overhead. First, compressive sensing tools are leveraged to construct channels at all the LIS elements from the channels seen only at the active elements. These full channels can then be used to design the LIS reflection matrices with no training overhead.

Abstract: Provided herein are synthetic nucleic acid molecules known as loop-mediated riboregulators that have single-nucleotide polymorphism (SNP) sensitivity and ultralow OFF state signal levels. Loop-mediated riboregulators can activate or repress gene expression in response to trigger RNAs bearing completely arbitrary sequences. Also provided herein are methods of using such synthetic nucleic acid molecules for detecting the presence or absence of a particular target RNA in, for example, a biological sample.

Abstract: A method for producing and recycling a crosslinked photopolymer is disclosed. The method includes mixing reactive thiols, multifunctional alkenes, and a photoinitiator to create a homogeneous mixture that is crosslinked through exposure to light. The method also includes decrosslinking the crosslinked photopolymer through base-catalyzed thiol-disulfide exchange reactions by mixing the crosslinked photopolymer with a reactive thiol, a base catalyst, and a solvent to create a decrosslinked material including recycled thiol oligomers which are reactive. The method further includes removing the base catalyst and the solvent and recrosslinking the recycled thiol oligomers by mixing stoichiometric amounts of the recycled thiol oligomers and a reactive alkene such that a molar ratio between thiol end groups and ene end groups is maintained at 1:1. The method includes adding a photoinitiator and mixing to create a homogeneous mixture, and recrosslinking the homogeneous mixture through exposure to light.

Abstract: A machine-learning framework for multi-fidelity modeling provides three components: multi-fidelity data compiling, multi-fidelity perceptive field and convolution, and deep neural network for mapping. This framework captures and utilizes implicit relationships between any high-fidelity datum and all available low-fidelity data using a defined local perceptive field and convolution. First, the framework treats multi-fidelity data as image data and processes them using a CNN, which is very scalable to high dimensional data with more than two fidelities. Second, the flexibility of nonlinear mapping facilitates the multi-fidelity aggregation and does not need to assume specific relationships among multiple fidelities. Third, the framework does not assume that multi-fidelity data are at the same order or from the same physical mechanisms (e.g., assumptions are needed for some error estimation-based multi-fidelity model).

Abstract: A dynamic nanophotonic filter and method for tuning and fabricating the same is disclosed. The filter includes a transparent substrate, a first layer of thermochromic VO2 deposed on the substrate with a first thickness, a spacer layer having a spacer thickness and composed of a dielectric material deposed on the first layer, and a second layer of thermochromic VO2 deposed on the spacer layer such that the spacer layer is sandwiched between the second and first layer. The dynamic nanophotonic filter changes between a semi-transparent state and an opaque state based on temperature. The semi-transparent state includes the first and second layers being insulating. The opaque state includes the first layer and the second layer both being metallic. The first thickness, the second thickness, and the spacer thickness are chosen to tune how the dynamic nanophotonic filter behaves in the semi-transparent state and the opaque states.