Abstract: The present invention relates to a method for the production of fatty acid esters which comprise unsaturated fatty acids with at least three double bonds, and to free unsaturated fatty acids with a content of at least 1% by weight based on the total fatty acids present in the plants, by expressing at least one nucleic acid sequence which encodes a polypeptide with ?6-desaturase activity and at least one nucleic acid sequence which encodes a polypeptide with ?6-elongase activity. Advantageously, these nucleic acid sequences can, if appropriate, be expressed in the transgenic plant together with a third nucleic acid sequence which encodes a polypeptide with ?5-desaturase activity. The invention furthermore relates to the use of defined nucleic acid sequences which encode polypeptides with a ?6-desaturase activity, ?6-elongase activity or ?5-desaturase activity selected from a group of nucleic acid sequences, and/or to the use of nucleic acid constructs comprising the abovementioned nucleic acid sequences.
Type:
Grant
Filed:
April 25, 2003
Date of Patent:
February 22, 2011
Assignee:
BASF Plant Science GmbH
Inventors:
Petra Cirpus, Andreas Renz, Jens Lerchl, Anne-Marie Kuijpers
Abstract: The invention relates to a plant seed oil, comprising arachidonic acid comprising approximately 7 to approximately 26 percent by weight of the total fatty acid content, the ratio of the percentages by weight of arachidonic acid to gamma-linolenic acid being approximately 1:1 to approximately 5:1 and the ratio of the percentages by weight of arachidonic acid to dihomo-gamma-linolenic acid being approximately 1:1 to approximately 5:1. The invention additionally relates to processes for the production of this plant seed oil and formulations and uses of the plant seed oil. In particular, the invention also makes available foodstuffs and baby food that contain the plant seed oil mentioned.
Type:
Application
Filed:
April 23, 2009
Publication date:
February 17, 2011
Applicant:
BASF Plant Science GmbH
Inventors:
Dietrich Rein, Toralf Senger, Jörg Bauer
Abstract: A transgenic plant transformed by a Protein Kinase Stress-Related Protein (PKSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated PKSRPs, and isolated nucleic acid coding PKSRPs, and vectors and host cells containing the latter.
Type:
Grant
Filed:
March 11, 2009
Date of Patent:
February 15, 2011
Assignee:
BASF Plant Science GmbH
Inventors:
Oswaldo da Costa e Silva, Nocha Van Thielen, Ruoying Chen, Hans J. Bohnert, Rodrigo Sarria-Millan
Abstract: The present invention relates to the field of agricultural biotechnology. Disclosed herein are methods, nucleic acid molecules and expression constructs or vectors thereof for an expression specific for the germinating embryo, transgenic plants and cells comprising such nucleic acids, vectors, expression constructs, as well as methods of making and using such DNA constructs and transgenic plant.
Abstract: Described herein are inventions in the field of genetic engineering of plants, including combinations of polynucleotides encoding LMPs to improve agronomic, horticultural, and quality traits. This invention also relates to the combination of polynucleotides encoding proteins that are related to the presence of seed storage compounds in plants. More specifically, the present invention relates to LMP polynucleotides encoding lipid metabolism proteins (LMP) and the use of these combinations of these sequences, their order and direction in the combination, and the regulatory elements used to control expression and transcript termination in these combinations in transgenic plants. In particular, the invention is directed to methods for manipulating fatty acid-related compounds and for increasing oil and starch levels and altering the fatty acid composition in plants and seeds.
Type:
Application
Filed:
December 10, 2008
Publication date:
February 10, 2011
Applicant:
BASF Plant Science GmbH
Inventors:
Gunnar Plesch, Piotr Puzio, Astrid Blau, Michael Manfred Herold, Birgit Wendel, Beate Kamlage, Florian Schauwecker, Thorsten Zank, Oliver Oswald, Tom Wetjen
Abstract: This invention relates generally to nucleic acid sequences encoding proteins that are associated with abiotic stress responses and abiotic stress tolerance in plants. This invention further relates to transformed plant cells with altered metabolic activity compared to a corresponding non transformed wild type plant cell, wherein the metabolic activity is altered by transformation with a Stress-Related Protein (SRP) coding nucleic acid and results in increased tolerance and/or resistance to an environmental stress as compared to a corresponding non-transformed wild type plant cell.
Abstract: A transgenic plant transformed by a Signal Transduction Stress-Related Protein (STSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated STSRPs, and isolated nucleic acid coding STSRPs, and vectors and host cells containing the latter.
Type:
Grant
Filed:
September 16, 2009
Date of Patent:
February 1, 2011
Assignee:
BASF Plant Science GmbH
Inventors:
Oswaldo da Costa e Silva, Nocha Van Thielen, Ruoying Chen, Hans J. Bohnert, Manabu Ishitani
Abstract: A transgenic plant transformed by a Lectin-like Protein Kinase Stress-Related Polypeptide (LPKSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased plant growth and/or increased tolerance to an environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated LPKSRPs, and isolated nucleic acids coding LPKSRPs, and vectors and host cells containing the latter.
Type:
Grant
Filed:
June 16, 2006
Date of Patent:
February 1, 2011
Assignee:
BASF Plant Science GmbH
Inventors:
Damian Allen, Lori V. Mills, Pilar Puente, Nocha van Thielen, Oswaldo da Costa e Silva
Abstract: The invention relates to a method for producing polyunsaturated fatty acids in an organism, according to which nucleic acids coding for polypeptides with an acyl-CoA:lysophospholipid-acyltransferase activity are introduced into the organism. Advantageously, the nucleic acid sequences can be expressed in the transgenic organism optionally together with other nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism. The invention also relates to the inventive nucleic acid sequences, nucleic acid constructs containing the inventive nucleic acid sequences, vectors containing the inventive nucleic acid sequences and/or the nucleic acid constructs, and transgenic organisms containing the nucleic acid sequences, nucleic acid constructs and/or vectors. The invention further relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use of the same.
Type:
Grant
Filed:
December 20, 2005
Date of Patent:
January 18, 2011
Assignee:
BASF Plant Science GmbH
Inventors:
Petra Cirpus, Jörg Bauer, Ernst Heinz, Amine Abbadi, Jelena Kirsch
Abstract: This invention relates generally to nucleic acid sequences encoding proteins that are related to the presence of seed storage compounds in plants. More specifically, the present invention relates to nucleic acid sequences encoding polypeptides being capable of altering the seed storage content and, in particular, oil, lipid and fatty acid metabolism regulator proteins and the use of these sequences in transgenic plants. In particular, the invention is directed to methods for manipulating seed storage compounds in plants and seeds. The invention further relates to methods of using these novel plant polypeptides to stimulate plant growth and/or to increase yield and/or composition of seed storage compounds.
Type:
Application
Filed:
August 22, 2008
Publication date:
January 13, 2011
Applicant:
BASF Plant Science GmbH
Inventors:
Thorsten Zank, Oliver Oswald, Tom Wetjen
Abstract: Polynucleotides are disclosed which are capable of enhancing a growth, yield under water-limited conditions, and/or increased tolerance to an environmental stress of a plant transformed to contain such polynucleotides. Also provided are methods of using such polynucleotides and transgenic plants and agricultural products, including seeds, containing such polynucleotides as transgenes.
Type:
Application
Filed:
November 27, 2008
Publication date:
December 30, 2010
Applicant:
BASF Plant Science GmbH
Inventors:
Amber Shirley, Damian Allen, Bryan D. McKersie, Nanfei Xu, Piotr Puzio, Richard Trethewey, Rodrigo Sarria-Millan, Amy McCaskill, Larissa Wilson, Lalitree Darnielle, Resham Kulkarni
Abstract: Isolated nucleic acids and proteins associated with lipid and sugar metabolism regulation are provided. In particular, lipid metabolism proteins (LMP) and encoding nucleic acids originating from Arabidopsis thaliana, Brassica napus, and Physcomitrella patens are provided. The nucleic acids and proteins are used in methods of producing trangenic plants and modulating levels of seed storage compounds. Preferably, the seed storage compounds are lipids, fatty acids, starches, or seed storage proteins.
Type:
Grant
Filed:
August 4, 2003
Date of Patent:
December 28, 2010
Assignee:
BASF Plant Science GmbH
Inventors:
Volker Mittendorf, Heiko A. Haertel, Joerg Bauer, Oliver Oswald
Abstract: A transgenic plant transformed by a Protein Kinase Stress-Related Protein (PKSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated PKSRPs, and isolated nucleic acid coding PKSRPs, and vectors and host cells containing the latter.
Type:
Grant
Filed:
August 24, 2009
Date of Patent:
December 28, 2010
Assignee:
BASF Plant Science GmbH
Inventors:
Oswaldo da Costa e Silva, Nocha Van Thielen, Ruoying Chen, Hans J. Bohnert, Rodrigo Sarria-Millan
Abstract: A transgenic plant transformed by a Protein Kinase Stress-Related Protein (PKSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated PKSRPs, and isolated nucleic acid coding PKSRPs, and vectors and host cells containing the latter.
Type:
Application
Filed:
September 1, 2010
Publication date:
December 23, 2010
Applicant:
BASF Plant Science GmbH
Inventors:
Oswaldo da Costa e Silva, Hans J. Bohnert, Nocha van Thielen, Ruoying Chen, Rodrigo Sarria-Millan
Abstract: The present invention relates generally to the field of molecular biology and concerns a method for increasing various plant yield-related traits by modulating expression in a plant of a nucleic acid sequence encoding a yield increasing polypeptide selected from the group consisting of: an AT-hook motif nuclear localized 19/20 (AHL19/20), a GRP (Growth Regulating Protein) (wherein said GRP polypeptide is a metallothionein 2a (MT2a) polypeptide), an alanine aminotransferase (AAT)-like polypeptide, and an alanine aminotransferase (AAT) polypeptide.
Abstract: A transgenic plant transformed by a Protein Kinase Stress-Related Protein (PKSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated PKSRPs, and isolated nucleic acid coding PKSRPs, and vectors and host cells containing the latter.
Type:
Grant
Filed:
August 24, 2009
Date of Patent:
December 21, 2010
Assignee:
BASF Plant Science GmbH
Inventors:
Oswaldo da Cosdta e Silva, Nocha Van Thielen, Ruoying Chen, Hans J. Bohnert, Rodrigo Sarria-Millan
Abstract: A transgenic plant transformed by a phosphatase stress-related protein (PHSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated PHSRPs, and isolated nucleic acid coding PHSRPs, and vectors and host cells containing the latter.
Type:
Grant
Filed:
February 13, 2009
Date of Patent:
December 7, 2010
Assignee:
BASF Plant Science GmbH
Inventors:
Oswaldo da Costa e Silva, Nocha Van Thielen, Ruoying Chen, Hans Bohnert, Manabu Ishitani
Abstract: This invention relates generally to nucleic acid sequences encoding proteins that are associated with abiotic stress responses and abiotic stress tolerance in plants. This invention further relates to transformed plant cells with altered metabolic activity compared to a corresponding non transformed wild type plant cell, wherein the metabolic activity is altered by transformation with a Stress-Related Protein (SRP) coding nucleic acid and results in increased tolerance and/or resistance to an environmental stress as compared to a corresponding non-transformed wild type plant cell.
Abstract: The present invention relates to a method for increasing the content of polyunsaturated long-chain fatty acids in an organism by introducing nucleic acids coding for polypeptides or proteins exhibiting a phospholipase, ketoacyl-CoA reductase and/or dehydratase activity. The present invention also provides nucleic acid sequences coding for polypeptides with enzyme activities of a phospholipase, ketoacyl-CoA reductase and/or dehydratase, and nucleic acid constructs, vectors and organisms containing the nucleic acid sequences according to the present invention. A further part of the present invention relates to oils, lipids and/or fatty acids produced according to the method of the present invention and the use thereof. Furthermore, the present invention relates to unsaturated fatty acids and triglycerides having an increased content of unsaturated fatty acids and the use thereof.
Type:
Grant
Filed:
July 15, 2005
Date of Patent:
November 30, 2010
Assignee:
BASF Plant Science GmbH
Inventors:
Petra Cirpus, Jörg Bauer, Xiao Qiu, Patricia Vrinten
Abstract: A transgenic plant transformed by a GTP Binding Stress-Related Protein (GBSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated GBSRPs, and isolated nucleic acid coding GBSRPs, and vectors and host cells containing the latter.
Type:
Grant
Filed:
March 19, 2007
Date of Patent:
November 23, 2010
Assignee:
BASF Plant Science GmbH
Inventors:
Ruoying Chen, Nocha Van Thielen, Oswaldo da Costa e Silva, Hans Bohnert