Abstract: Embodiments of the disclosure include methods and compositions in which two inter-chromosomal or intra-chromosomal genomic DNA regions are rearranged upon exposure of the genomic DNA to a chimeric RNA that produces fusion of the two regions. The chimeric RNA hybridizes to the two different parts of the genomic DNA and forms a DNA/RNA hybrid in a sequence-specific manner, thereby facilitating genomic rearrangement. In particular embodiments this mechanism is utilized to produce directed gene fusion in targeted genomic DNA regions.

Abstract: The disclosure provides a system and method for ultrasonic non-destructive testing to monitor the curing process of a resin and filled resin materials during manufacture. The inventors have discovered that ultrasound parameters revealed in ultrasonic signal analysis reveals the degree of cure and therefore important curing stages during processing for a proper cure and allow the product to perform as intended. Generally, the ultrasound parameters include signal speed of sound and/or instantaneous peak power relative to wall-clock time of the ultrasound signal. The system and method can monitor the cure of resin and filled resin materials using instantaneous data without the need for previous data for that individual scan, can use ultrasound portably such that carbon fiber laminates can be monitored in-situ, and is conducive to isothermal cure or cure using a varying temperature profile. Thus, the invention represents a step in manufacturing of resin-curing products heretofore unavailable.

Abstract: Equipotent indolocarbazole-derived analogs of staurosporine identified herein are prepared through C—H borylation chemistry. Functionality resides at C2 and C10 of the indolocarbazole aromatic region. Introducing functionality in this previously inaccessible region does not abrogate kinase activity and is shown to change the selectivity profile.

Abstract: A portable ventilator device suitable for emergency use, such as cardiac pulmonary resuscitation and well as ventilation in non-cardiac induced medical events. The device is connected to a display screen that receives simple inputs from an operator, including height, weight, or sex of the distressed patient, but does not require input of more complicated variables, such as tidal volume, respiratory rate, inspiratory airflow, or positive end-expiratory pressure. Based on the input height, weight, and/or sex, the device automatically correlates the input values with probably values for tidal volume, respiratory rate, inspiratory airflow, or positive end-expiratory pressure, based previous data point correlations. The device then begins delivering air from a ventilator to the patient based on the estimated, correlated values. While the device utilizes simple inputs, it still capable of operating in multiple modes with both pressure control and volume control.

Abstract: G protein-coupled receptor (GPCR) regulators and methods for their use are provided herein. The GPCR regulators described herein are useful in treating and/or preventing conditions or diseases associated with a GPCR, including ageing, cancer, cardiovascular disorders, hematologic disorders, infectious diseases, inflammatory diseases, metabolic diseases, neurodegenerative disorders, respiratory diseases, or urological disorders. Also provided are methods of regulating a G protein-coupled receptor in a cell using the compounds and compositions described herein.

Abstract: The disclosure provides a diamagnetically stabilized magnetically levitated gravimeter and related method that allows measurements of relative gravity in a simple, low power consumption device based on a magnetic levitation principle using permanent magnets instead of using a mechanical spring. The gravimeter uses magnetic forces to balance a float magnet against the force of gravity, allowing for accurate measurements. The gravimeter includes a float magnet that floats between two diamagnetic materials, such as diamagnetic plates, without a need for external energy input due to the interaction between the magnetic forces of the float magnet lifted by the lift magnet but stabilized between upper and lower diamagnetic materials. The gravimeter is less sensitive to drift in response to stresses than a mechanical spring, have a lower temperature sensitivity, and lower energy and power requirements to take similarly reliable gravity measurements, which in turn simplify deployment and prolong operational lifetime.

Abstract: Embodiments of the disclosure include a tissue and/or access device that allows for adjustability of placement of a needle for accessing a blood vessel of an individual in need thereof, particularly when used with an imaging device. The adjustability originates from both angle of the needle for insertion into the body of the individual and longitudinal placement of the needle on the body of the individual. In specific embodiments, a needle guide through which the needle traverses is detachable.

Abstract: Novel small molecule proteolysis-targeting chimeras (PROTACs) are provided, along with methods for their use as Bruton's tyrosine kinase (BTK) degraders. The small molecule PROTACs described herein are useful in treating and/or preventing BTK-related diseases, such as cancer, neurodegenerative disorders, inflammatory diseases, and metabolic disorders. Also provided are methods for inducing BTK degradation in a cell using the compounds and compositions described herein.

Abstract: Embodiments of the disclosure include high-throughput profiling of transcriptomes of subcellular compartments or structures, including a droplet-based single-cell total-RNA-seq method that enables profiling of transcripts localized in particular subcellular compartment or structures. In specific embodiments, the disclosure provides for transcriptome profiling of single nuclei that allows for construction of a cell atlas using only long non-coding RNA species that can be applied for tissue-wide identification of cell-type-specific lncRNA species.

Abstract: Embodiments of the present disclosure pertain to isolated peptides of any one of SEQ ID NOS: 1-14, derivatives thereof, analogs thereof, homologs thereof, and combinations thereof. The isolated peptides may be in aggregated form, fibrillated form, in the form of three-dimensional hydrogels, or combinations thereof. Additional embodiments of the present disclosure pertain to methods of delivering the isolated peptides of the present disclosure into cells by exposing the cells to the isolated peptides and/or nucleotide sequences that express them. In some embodiments, the exposing results in the conversion of the cells to pluripotent stem cells. In some embodiments, the exposing occurs in vitro. In some embodiments, the exposing occurs in vivo in a subject by administering the isolated peptides and/or nucleotide sequences of the present disclosure to the subject.

Abstract: The present disclosure provides a system and method for real-time visualization of a material during ultrasonic non-destructive testing. The system includes a graphical user interface (GUI) capable of showing a three-dimensional (3-D) image of a composite laminate constructed of a series of two-dimensional (2-D) cross sections. The GUI is capable of displaying the 3-D image as each additional 2-D cross section is scanned by an ultrasonic testing apparatus in real time or near real time, including probable defect regions that contain a flaw such as a hole, crack, wrinkle, or foreign object within the composite. Furthermore, in one embodiment, the system includes an artificial intelligence capable of highlighting defect areas within the 3-D image in real time or near real time and providing data regarding each defect area, such as the depth, size, and/or type of each defect.

Abstract: Systems and methods for a multi-primary color system for display. A multi-primary color system increases the number of primary colors available in a color system and color system equipment. Increasing the number of primary colors reduces metameric errors from viewer to viewer. A six-primary color system includes Red, Green, Blue, Cyan, Yellow, and Magenta primaries. The systems of the present invention maintain compatibility with existing color systems and equipment and provide systems for backwards compatibility with older color systems.

Abstract: The present invention includes systems and methods for a multi-primary color system for display. A multi-primary color system increases the number of primary colors available in a color system and color system equipment. Increasing the number of primary colors reduces metameric errors from viewer to viewer. One embodiment of the multi-primary color system includes Red, Green, Blue, Cyan, Yellow, and Magenta primaries. The systems of the present invention maintain compatibility with existing color systems and equipment and provide systems for backwards compatibility with older color systems.

Abstract: The present disclosure provides a system and method for real-time visualization of a material during ultrasonic non-destructive testing. The system includes a graphical user interface (GUI) capable of showing a three-dimensional (3-D) image of a composite laminate constructed of a series of two-dimensional (2-D) cross sections. The GUI is capable of displaying the 3-D image as each additional 2-D cross section is scanned by an ultrasonic testing apparatus in real time or near real time, including probable defect regions that contain a flaw such as an air pocket, delamination, or foreign object within the composite. Furthermore, in one embodiment, the system includes an artificial intelligence capable of highlighting foreign objects within the 3-D image in real time or near real time and providing data regarding each object area, such as the depth, size, and/or type of each defect.

Abstract: Systems and methods for a multi-primary color system for display. A multi-primary color system increases the number of primary colors available in a color system and color system equipment. Increasing the number of primary colors reduces metameric errors from viewer to viewer. One embodiment of the multi-primary color system includes Red, Green, Blue, Cyan, Yellow, and Magenta primaries. The systems of the present invention maintain compatibility with existing color systems and equipment and provide systems for backwards compatibility with older color systems.

Abstract: The present disclosure encompasses methods for generating cells or tissue from existing cells with one or more mutated variants of Yap. In specific embodiments, the disclosure regards treatment of existing cardiomyocytes with one or more mutated variants of Yap that causes them to divide and generate new cardiomyocytes. In specific cases, the mutated variant of Yap has serine-to-alanine substitutions at 1, 2, 3, 4, 5, 6, or more serines of Yap.

Abstract: Polymeric nanoparticles encapsulating microRNA are prepared according to described methods. The nanoparticles serve as an improved drug delivery system for treatment of cardiovascular disease.

Abstract: Systems and methods for a multi-primary color system for display. A multi-primary color system increases the number of primary colors available in a color system and color system equipment. Increasing the number of primary colors reduces metameric errors from viewer to viewer. One embodiment of the multi-primary color system includes Red, Green, Blue, Cyan, Yellow, and Magenta primaries. The systems of the present invention maintain compatibility with existing color systems and equipment and provide systems for backwards compatibility with older color systems.

Abstract: A novel network of tumorigenic prognostic factors is identified that plays a critical role in advanced pancreatic cancer (PC) pathogenesis. This interactome is interconnected through a central tumor suppressive microRNA, miR-198, which is able to both directly and indirectly modulate expression of the various members of this network to alter the molecular makeup of pancreatic tumors, with important clinical implications. When this tumor signature network is intact, miR-198 expression is reduced and patient survival is dismal; patients with higher miR-198 present an altered tumor signature network, better prognosis and increased survival. Further, according to the present disclosure, MiR-198 replacement reverses tumorigenicity in vitro and in vivo.

Abstract: Embodiments of the disclosure include methods and compositions for in situ cardiac cell regeneration, including transdifferentiation of cardiac cells to cardiomyocytes. In particular embodiments, in situ cardiac cell regeneration encompasses delivery of p63-TID and one or both of Hand2 and myocardin, and in specific embodiments further includes one or more of Gata4, Mef2c, and Tbx5, and/or one or more of ETV2 and VEGF. In specific aspects of the disclosure, adult cardiac fibroblasts are reprogrammed into cardiomyocytes using viral vectors that harbor p63-TID and one or both of the transcription factors Hand2 and myocardin.