Abstract: Disclosed are a super-resolution imaging system (1, 41, 51), a super-resolution imaging method, a biological sample identification system (4, 61) and method, a nucleic acid sequencing imaging system (5) and method, and a nucleic acid identification system (6) and method. The super-resolution imaging system (1, 41, 51) includes an illumination system (A) and an imaging system (B). The illumination system (A) outputs excitation light to irradiate a biological sample to generate excited light, and the imaging system (B) collects and records the excited light to generate an excited light image. The illumination system (A) includes an excitation light source (10, 10a) and a structured light generation and modulation device (11, 11a). The excitation light source (10, 10a) outputs the excitation light, and the structured light generation and modulation device (11, 11a) modulates the excitation light into structured light to irradiate the biological sample to generate the excited light.

Abstract: Provided are a magnetic bead-based detection method, a storage medium, and a detection device. The detection method includes: collecting a white light image of a to-be-detected solution, in which the to-be-detected solution is mixed with a to-be-detected sample and magnetic beads with a capture agent (S1); determining magnetic stripe regions in the white light image, and determining first magnetic bead regions based on the magnetic stripe regions (S2); selecting, by using a first neural network, second magnetic bead regions containing magnetic beads from the first magnetic bead regions, and obtaining a marker position of each of the magnetic beads (S3); and obtaining, by using a second neural network and based on each of the second magnetic bead regions, codes at code bits of a corresponding magnetic bead, and obtaining corresponding code information based on the codes of the code bits and the marker position of the magnetic bead (S4).

Abstract: A biological sample image collection device (100), comprising support (30) and an optical imaging assembly (50), also comprises: a plurality of movable platforms (40) for placing biological samples (20), wherein the plurality of movable platforms (40) are arranged on the support (30) in an array; the plurality of movable platforms (40) can move relative to the support (30); and forces acting on the support (30) during the movement of the movable platforms can cancel each other out, so as to avoid vibrations affecting the support (30) and the biological samples (20) are canceled. The optical imaging assembly (50) collects images of the biological samples (20) on the movable platforms (40) when the plurality of movable platforms (40) move, relative to the center of the array, in the same direction and at the same speed. Further provided is a gene sequencer including the biological sample image collection device (100).

Abstract: Provided in the present disclosure is a method for sequencing a double-stranded target polynucleotide. The method can keep ATP at a relatively constant concentration during sequencing, so that the sequencing rate can be better kept stable and unchanged. Further provided in the present disclosure is a kit for sequencing a double-stranded target polynucleotide, the kit including a transmembrane pore in the membrane, a helicase, an ATP-generating enzyme and an ATP-generating substrate.

Abstract: Provided is a use of Megamonas funiformis in the prevention and/or treatment of metabolic diseases. Megamonas funiformis has the function of preventing and/or treating metabolic diseases (such as obesity, diabetes, atherosclerosis-related diseases, cardiovascular disease and hyperuricemia).

Abstract: A nucleic acid synthesis device and a nucleic acid purification device, uses thereof, and a nucleic acid synthesis method and a nucleic acid purification method. The nucleic acid synthesis device includes a solid support, and the solid support includes a controlled pore glass (CPG), the CPG is an unmodified and bare CPG, a surface of the CPG has a hydroxyl group, and the hydroxyl group is attachable, though a covalent bond, to a phosphoramidite-protected nucleotide monomer or multimer for synthesis of nucleic acid. The nucleic acid synthesis device of the present disclosure can be used for not only synthesis of an oligonucleotide primer, but also for purification of enzymatic digestion and PCR product by using the oligonucleotide primer immobilized on the CPG, and has advantages of simple structure, small volume, light weight, high efficiency, low costs, and diversified functions.

Abstract: A gene chip includes a chip carrier, a plurality of DNA nanoballs assembled on the chip carrier, and a polymer film formed on the chip carrier and wrapping the DNA nanoballs. The polymer film includes at least one of a film of a positively charged polymer, a film of a positively charged polymer which is modified, a film of a zwitterionic polymer, and a composite polymer film. The composite polymer film is formed by a layer-by-layer self-assembly process of a positively charged polymer and a negatively charged polymer. The gene chip has good sequencing quality and different functions can be achieved by coating with different polymers, such as the chip surface rapidly drying out and surface non-specific adsorption. A method of preparing a gene chip is further disclosed.

Abstract: Reversibly blocked nucleoside analogues and methods of using such nucleoside analogues for sequencing of nucleic acids are provided.

Abstract: A method for predicting the relationship between a bacteriophage and a bacterial infection, comprising a learning model for a bacteriophage-host infection relationship and a construction method therefor. The method for constructing a learning model for a bacteriophage-host infection relationship comprises: training a learning model by using data of a training set of bacteriophages and bacterial strains, the data of the training set of bacteriophages and bacterial strains comprising genome data and phenotypic assay data. A method for using the learning model for a bacteriophage-host infection relationship to predict the bacteriophage that will infect bacteria and a method for predicting the bacteria infected by a bacteriophage, and a computer-executable medium comprising a computer program for performing the methods.

Abstract: Provided is a recombinant KOD polymerase, which is the following A) or B): the polymerase shown in A) is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 18 positions in a wild-type KOD DNA polymerase amino acid sequence: 675th, 385th, 710th, 674th, 735th, 736th, 606th, 709th, 347th, 349th, 590th, 676th, 389th, 589th, 680th, 384th, 496th and 383rd; the polymerase described by B) is a protein having DNA polymerase activity that is derived from A) by adding a tag sequence to an end of the amino acid sequence of the protein shown in A).

Abstract: Provided is a MART-1 (27-35) epitope-specific T cell receptor, comprising an ? chain and a ? chain. The ? chain comprises three complementary determining regions, respective sequences thereof being positions 61-66, positions 84-89, and positions 124-136 of SEQ ID No. 3. The ? chain comprises three complementary determining regions, respective amino acid sequences thereof being positions 46-50, positions 68-73, and positions 112-125 of SEQ ID No. 4. A T cell expressing the TCR can effectively recognize a MART-1 (27-35) epitope polypeptide supported on a T2 cell and secrete IFN-?, thereby demonstrating the functionality of the receptor. Use of the TCR with a relevant drug target allows for effective drug development.

Abstract: The present invention discloses an application of Alistipes shahii in preparing a composition for preventing and/or treating lipid metabolism related diseases, for example but not limited to, atherosclerosis related diseases, cardiovascular diseases and obesity.

Abstract: A method for constructing a library of cell-free DNAs in body fluids, comprising directly acting a transposase or an endonuclease on a body fluid sample, fragmenting the cell-free DNAs within, and performing amplification to obtain a library. Also provided is a test kit using the present method for prenatal diagnosis or early detection of cancer.

Abstract: A lens-free microscopic imaging system (11), which is used to image microbeads (15) with pattern codes, includes an illumination system (11a) and an imaging system (11b). The illumination system (11a) includes an illumination light source (111) and an excitation light source (112). The imaging system (11b) includes an image sensor (113). The illumination light source (111) is used to emit illumination light to irradiate the microbeads (15), causing the irradiated microbeads (15) to be imaged on the image sensor. The excitation light source (112) is used to emit excitation light to excite the microbeads (15) to generate specific signals. The image sensor (113) is used to collect the images of the microbeads (15) and the specific signals to generate images. The imaging system (11) does not require a lens system. The present disclosure improves a detection efficiency of the microbeads (15).

Abstract: Provided are a magnetic bead-based detection method, a storage medium, and a detection device. The detection method includes: collecting a white light image of a to-be-detected solution, in which the to-be-detected solution is mixed with a to-be-detected sample and magnetic beads with a capture agent (S1); determining magnetic stripe regions in the white light image, and determining first magnetic bead regions based on the magnetic stripe regions (S2); selecting, by using a first neural network, second magnetic bead regions containing magnetic beads from the first magnetic bead regions, and obtaining a marker position of each of the magnetic beads (S3); and obtaining, by using a second neural network and based on each of the second magnetic bead regions, codes at code bits of a corresponding magnetic bead, and obtaining corresponding code information based on the codes of the code bits and the marker position of the magnetic bead (S4).

Abstract: Techniques are described for preparing monodisperse polymer beads, such as using monosized seed particles, where the process of growing, polymerizing, or swelling the beads during preparation occurs at low, ambient, or room temperature. A variety of schemes are disclosed for growing, polymerizing, or swelling that avoid thermal initiation-based swelling. For example, photo polymerization, metal-free atom transfer radical polymerization, and redox polymerization schemes are disclosed. Additional features may be implemented in the monodisperse polymer beads, such as controlling bead density, controlling porous character, and inclusion of various chemical functionalities, including protected functionalities that can be activated or de-protected after preparation of the monodisperse polymer beads, such as during subsequent use.

Abstract: The present disclosure provides a fluorescence image registration method, the method includes: acquiring a fluorescence image of a biochip; selecting a preset local region of the fluorescence image; acquiring a position of a minimum value of a sum of brightness values of pixels in a first direction and a second direction, and obtaining pixel-level registration points; dividing the pixel-level registration points into non-defective pixels and defective pixels; if the fluorescence image meets the preset standard, correcting positions of the defective pixels according to positions of the non-defective pixels; acquiring a position of a center of gravity of image points of fluorescent molecules according to a center of gravity method; fitting straight lines in the first direction and the second direction respectively according to the position of the center of gravity; and acquiring boundary points of the fluorescence image and calculating the positions of the boundary points.

Abstract: Use of Anaerofustis stercorihominis in the prevention and/or treatment of metabolic diseases such as obesity, diabetes, atherosclerosis-related diseases, and cardiovascular diseases.

Abstract: The present invention relates to the field of biotechnology. A method and system of detecting fetal chromosomal abnormalities are disclosed.

Abstract: A method for generating a digital identity is provided, including: extracting a first preset number of short tandem repeat STRs and relevant information of each STR from whole genome data; generating a single STR digital code corresponding to each STR according to the relevant information of each STR, to obtain a plurality of single STR digital codes; performing sequence transformation on each single STR digital code with a preset rule, and generating a target STR digital code according to the single STR digital code after the sequence transformation; generating summary information of the target STR digital code, and determining the summary information as summary information of the STR to which the target STR digital code belongs; and determining the summary information of the STR as the generated digital identity.