Abstract: Disclosed are a conotoxin polypeptide ?-CPTx-bt105, a method for preparation thereof, and an application thereof. The conotoxin polypeptide of the present invention consists of 16 amino acids, has a molecular weight of 1626.62 daltons, and has the full sequence GICCVDDTCTTHSGCL (SEQ. ID NO. 1).

Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.

Abstract: A method for encoding and storing text information using DNA as a storage medium, a decoding method therefor and an application thereof. The method for using DNA to store text information comprises: encoding characters into computer binary digits by means of encoding, and converting the binary digits into DNA sequences by means of transcoding; and artificially synthesizing the DNA sequences encoded with character information, positioning the characters by means of a designed ligation adapter, and assembling the DNA sequences encoded with the character information according to a pre-set order. The method for using DNA to store text information has the advantages of a small storage volume, a large storage capacity, a strong stability and low maintenance costs.

Abstract: Provided are Christensenella intestinihominis and an application thereof, specifically, use of Christensenella intestinihominis in treating and preventing obesity and related diseases thereof. Also provided are a composition for treating and preventing obesity and related diseases thereof, comprising compositions of drugs, drinks, food, or animal feed, and a method for reducing weight and/or blood lipid.

Abstract: Provided are Faecalibacterium longum and an application thereof, specifically, use of Faecalibacterium longum in treating and preventing diabetes and related diseases thereof. Also provided are a composition for treating and preventing diabetes and related diseases thereof, comprising compositions of drugs, drinks, food, or animal feed, and a method for reducing weight, fasting plasma glucose and/or blood lipid, and improving glucose tolerance.

Abstract: Disclosed are a conotoxin polypeptide ?-CPTx-bt103, a method for preparation thereof, and an application thereof. The conotoxin polypeptide of the present invention consists of 29 amino acids, has a molecular weight of 3141.43 daltons, and has the full sequence RTNCGETCLKDEQCVGACQICVPSQLKCL (SEQ ID NO. 1).

Abstract: Disclosed are a conotoxin polypeptide ?-CPTx-bt104, a method for preparation thereof, and an application thereof. The conotoxin polypeptide of the present invention consists of 11 amino acids, has a molecular weight of 1313.47 daltons, and has the full sequence SLCCPEDRWCC (SEQ. ID NO. 1).

Abstract: Improved single molecule sequencing methods, compositions, and devices, are provided. In a first aspect, the present invention provides a multi-pass method of sequencing a target sequence using nanopore sequencing, the method comprising: i) providing a non-naturally occurring concatemer nucleic acid molecule comprising a plurality of copies of the target sequence; ii) nanopore sequencing at least three copies of the target sequence in the concatemer, thereby obtaining a multi-pass sequence dataset, wherein the multi-pass sequence dataset comprises target sequence datasets for the at least three copies of the target sequence; and iii) using the multi-pass sequence dataset to determine the target sequence.

Abstract: Provided are a conotoxin peptide ?-CPTx-bt103 and a derivative polypeptide. The amino acid sequence of the conotoxin peptide is indicated by SEQ ID NO: 1. Also provided are a conotoxin peptide preparation method and uses of the conotoxin peptide in the treatment of diseases related to a calcium ion channel.

Abstract: Biomarkers and methods related to microbiota for predicting the risk of a disease, particularly colorectal cancer (CRC), are described.

Abstract: Provided is a new conotoxin peptide ?-CPTx-bt101. The conotoxin peptide ?-CPTx-bt101 has derivative polypeptide that has an amino acid sequence indicated by SEQ ID NO:1, or has derivative polypeptide that has an amino acid sequence indicated by SEQ ID NO:1 and that is formed after substitution, addition or deletion of one or more amino acids are performed and has original functions. Also provided is polynucleotide for coding the peptide, comprising a constructed body of the polynucleotide, an expression carrier and transformed cells. A peptide preparation method and uses in the treatment of diseases related to a calcium ion channel.

Abstract: Disclosed are a stabilizer for storage of a biological sample, a composition for storage of a nucleic acid and/or a polypeptide in a biological sample under non-freezing conditions, a kit comprising the composition, a mixture comprising the composition and a biological sample, and a method for storage of a nucleic acid and/or a polypeptide in a biological sample under non-freezing conditions. The integrity and stability of the nucleic acid and/or polypeptide in a biological sample can be stored for a long time under non-freezing conditions by using the stabilizer according to the invention, and therefore the stabilizer has a good application prospect.

Abstract: Provided is a method of constructing a sequencing library. The method includes 1) providing a single-stranded DNA fragment from a biological sample; 2) subjecting the single-stranded DNA fragment to whole genomic amplification to obtain a whole genome amplification product; 3) fragmenting the whole genome amplification product using a transposase embedded with two adaptors to obtain a fragmented product with two adaptors respectively at two ends; and 4) amplifying the fragmented product with two adaptors respectively at two ends using a tag sequence and a pair of primers to obtain said sequencing library.

Abstract: Provided is a new conotoxin peptide ?-CPTx-btl02. The conotoxin peptide ?-CPTx-btl02 has derivative polypeptide that has an amino acid sequence indicated by SEQ ID NO:1, or has derivative polypeptide that has an amino acid sequence indicated by SEQ ID NO:1 and that is formed after substitution, addition or deletion of one or more amino acids are performed and has original functions. Also provided is polynucleotide for coding the peptide, comprising a constructed body of the polynucleotide, an expression carrier and transformed cells. A peptide preparation method and uses in the treatment of diseases related to a calcium ion channel.

Abstract: Provided are a conotoxin peptide ?-CPTx-btl04 and a derivative polypeptide. The amino acid sequence of the conotoxin peptide is indicated by SEQ ID NO: 1. Also provided are a conotoxin peptide preparation method and uses of the conotoxin peptide in the treatment of diseases related to a calcium ion channel.

Abstract: Disclosed is a high-throughput detection method for a DNA synthesis product. According to the method of the present invention, a high-throughput sequencing technology is applied to detection of a DNA chip synthesis product through a mixed library construction strategy, so that costs of product detection and accurate product screening are reduced.

Abstract: An intestinal microorganism metagenomic feature is a bacteroid intestinal pattern. By using the intestinal metagenomic pattern, it was found that type 2 diabetic patients with different intestinal parasitic bacterial flora had remarkably different responses to treatment with the diabetic hypoglycemic drug acarbose. Therefore, before treatments, the intestinal patterns of the patients can be assessed to select those patients likely to experience optimal curative effects, and to determine whether acarbose is suitable for the treatment of individual patients with type 2 diabetes. In addition, while intestinal patterns are conventionally distinguished by DNA sequencing or PCT amplification of parasitic bacteria in excrements, the intestinal patterns can be well distinguished using a bile acid composition, especially secondary bile acids, under baseline conditions. The intestinal patterns can be identified through markers in blood, and then be used as markers for diagnosis.

Abstract: Biomarkers for diabetes and usages thereof are provided. And the biomarkers are nucleotides having polynucleotide sequences defined in SEQ ID NOs: 1-50.

Abstract: Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.

Abstract: Improved single molecule sequencing methods, compositions, and devices, are provided. In a first aspect, the present invention provides a multi-pass method of sequencing a target sequence using nanopore sequencing, the method comprising: i) providing a non-naturally occurring concatemer nucleic acid molecule comprising a plurality of copies of the target sequence; ii) nanopore sequencing at least three copies of the target sequence in the concatemer, thereby obtaining a multi-pass sequence dataset, wherein the multi-pass sequence dataset comprises target sequence datasets for the at least three copies of the target sequence; and iii) using the multi-pass sequence dataset to determine the target sequence.