Patents Assigned to Bio-Rad Laboratories, Inc.
  • Patent number: 10732088
    Abstract: Disclosed is a sheath delivery system that uses a continuous flow of sheath fluid into a pressurized internal reservoir that substantially matches the outflow of sheath fluid through the nozzle of a flow cytometer. A substantially constant level of the sheath fluid is maintained. If the sheath fluid level falls below a desired level, or goes above a desired level, a dampened control system is used to reach the desired level. In addition, air pressure in the pressurized internal container is controlled so that an external sheath container can be removed and refilled with additional sheath fluid without stopping the sheath delivery system 100. Differences in pressure are detected by a droplet camera, which measures the droplet breakoff point to determine the pressure of the sheath fluid in the nozzle.
    Type: Grant
    Filed: July 9, 2018
    Date of Patent: August 4, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Daniel Nelson Fox, Nathan Michael Gaskill-Fox
  • Patent number: 10730030
    Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.
    Type: Grant
    Filed: January 6, 2017
    Date of Patent: August 4, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ronald Lebofsky, Nicholas Heredia
  • Patent number: 10732145
    Abstract: Gel electrophoresis receptacles and methods of using such receptacles are provided. In one embodiment, a receptacle comprises a cavity for receiving an electrophoresis gel, wherein at least a portion of the receptacle is formed from cyclic olefin copolymer or cyclic olefin polymer.
    Type: Grant
    Filed: September 22, 2016
    Date of Patent: August 4, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Clayton McKee, Cory Panattoni, Paul Liu
  • Patent number: 10730045
    Abstract: The present invention generally pertains to a system for performing droplet inflation, and methods and kits comprising the same. The system comprises at least one microfluidic channel comprising one or more droplets flowing therein, one or more fluid reservoirs, one or more inflators, one or more inflator nozzles, and at least one mechanism for disrupting an interface between a droplet and a fluid. The present invention provides for the inflation of a relatively controlled volume of fluid into a droplet resulting in an increase in the volume of the droplet relative to its volume prior to inflation and, accordingly, dilution of the concentration of species, if any, previously present and emulsified in the droplet.
    Type: Grant
    Filed: November 20, 2018
    Date of Patent: August 4, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Tony Hung, Adnan Esmail, Sepehr Kiani
  • Patent number: 10724082
    Abstract: The invention generally relates to methods for increasing the amount of DNA available for analysis when using partitioned samples and parallel processing. For example, double-stranded DNA can be dissociated into two single-stranded components, and the single strands partitioned into different droplets prior to analysis. The disclosed methods are useful for performing digital PCR analysis on samples where the target DNA is not in abundance, for example when the sample originates from a body fluid or an FFPE sample.
    Type: Grant
    Filed: December 8, 2017
    Date of Patent: July 28, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Michael Samuels
  • Patent number: 10724084
    Abstract: A fluorescence detection apparatus for analyzing samples located in a plurality of wells in a thermal cycler and methods of use are provided. In one embodiment, the apparatus includes a support structure attachable to the thermal cycler and a detection module movably mountable on the support structure. The detection module includes one or more channels, each having an excitation light generator and an emission light detector both disposed within the detection module. When the support structure is attached to the thermal cycler and the detection module is mounted on the support structure, the detection module is movable so as to be positioned in optical communication with different ones of the plurality of wells. The detection module is removable from the support structure to allow easy replacement.
    Type: Grant
    Filed: December 28, 2018
    Date of Patent: July 28, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Igor Kordunsky, Jeffrey A. Goldman, Michael J. Finney
  • Patent number: 10718007
    Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.
    Type: Grant
    Filed: November 28, 2016
    Date of Patent: July 21, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, Priyadarshini Gogoi, Saedeh Sepehri Javdani, Yi Zhou
  • Patent number: 10718008
    Abstract: Methods of detecting or quantifying short RNA or DNA molecules using split cycle amplification are provided.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: July 21, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Dianna Maar, Samantha Cooper, Wei Yang
  • Patent number: 10697881
    Abstract: Disclosed is a sampler that obtains fluid samples with a probe and washes the probe during the act of withdrawing the probe from a sample container. In addition, detection of a collision of the probe and/or the wash station with another object can be quickly detected using sensors that determine if a collision situation exists.
    Type: Grant
    Filed: February 9, 2017
    Date of Patent: June 30, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Rodney C. Harris
  • Patent number: 10697981
    Abstract: Methods are provided for end users of diagnostic measurement procedures to prepare quality controls having desired analyte recoveries, estimate recoveries of quality controls already prepared, and compare estimated and measured recoveries. To prepare a quality control containing a particular analyte, a desired recovery of a measurement procedure for the analyte can be scaled by a correlation factor to obtain a target nominal concentration of the analyte in the quality control. Alternatively, the nominal concentration of an analyte in a quality control can be scaled by a correlation factor to obtain a predicted recovery of a measurement procedure for the analyte. The correlation factors can be based on recovery data previously obtained using the measurement procedure and optionally one or more reference procedures, and can be calculated using regression analysis of these data.
    Type: Grant
    Filed: December 20, 2017
    Date of Patent: June 30, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Alireza Ebrahim, Karl De Vore, Christopher Spates
  • Patent number: 10688487
    Abstract: Lateral flow devices, methods and kits for performing lateral flow assays are provided.
    Type: Grant
    Filed: November 20, 2017
    Date of Patent: June 23, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: William Strong, Clayton T. McKee
  • Patent number: 10690650
    Abstract: A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.
    Type: Grant
    Filed: October 1, 2019
    Date of Patent: June 23, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, Kyle Gleason, Christopher Siemer, Priyadarshini Gogoi, Saedeh Sepehri Javdani, Gene Parunak, Aaron Kehrer, Jon Meines
  • Patent number: 10682789
    Abstract: A method for efficiently manufacturing and fabricating microfluidic chips, where a base mold is formed to have positive-relief features used to cast an intermediary template chip with negative-relief features having dimensions of a scale in the micron range. The intermediary template chip is used to case a production mold, which is formed of a reinforced epoxy resin that, once hardened into a solid epoxy member, can withstand the structural pressures of a CNC machining system. The production mold can be refined by a CNC machining, where the refined production mold is then used to cast production chips to be used as microfluidic chips.
    Type: Grant
    Filed: August 28, 2015
    Date of Patent: June 16, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Daniel Konstantinou, Alwin Wan, Tal Rosenzweig, Amir Sadri, Nenad Kircanski, Edmond Young
  • Patent number: 10682647
    Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In an exemplary method, a sample-containing fluid may be dispensed into a well through a sample port of a channel component. The channel component may include (a) a body having a bottom surface attached to the well, and a top surface with a microchannel formed therein, and (b) an input tube projecting into the well from the bottom surface of the body. The sample-containing fluid when dispensed may contact a bottom end of the input tube and may be retained, with assistance from gravity, out of contact with the microchannel. A pressure differential may be created that drives at least a portion of the sample-containing fluid from the well via the input tube and through the microchannel.
    Type: Grant
    Filed: June 26, 2017
    Date of Patent: June 16, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Anthony J. Makarewicz, Jr., Luc Bousse, Stefano Schiaffino
  • Patent number: 10682640
    Abstract: Solid supports and ligands are provided for purification of biomolecules by mixed-mode anion exchange-hydrophobic chromatography. Compositions can have the formula Support-(X)—N(R1, R2)-R3-L-Ar, or a salt thereof, wherein: Support is a chromatographic solid support; X is a spacer or absent; R1 and R2 are each selected from hydrogen and an alkyl comprising 1-6 carbons; R3 is an alkyl comprising 1-6 carbons or a cyclo alkyl comprising 1-6 carbons; L is NR4, O, or S; wherein R4 is hydrogen or an alkyl comprising 1-6 carbons; and Ar is an aryl. Methods are also provided for using solid supports and ligands to purify biomolecules such as monomeric antibodies.
    Type: Grant
    Filed: April 28, 2017
    Date of Patent: June 16, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Jiali Liao, Russell Frost
  • Patent number: 10683551
    Abstract: This invention provides methods of determining the biological, pathological, genetic, epigenetic or disease status in a biological sample by determining the methylation status of a subpopulation of genomic DNA in the sample.
    Type: Grant
    Filed: January 20, 2017
    Date of Patent: June 16, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Steven T. Okino, Yan Wang
  • Patent number: 10676736
    Abstract: Methods and compositions are provided herein for preparing high-throughput cDNA sequencing libraries.
    Type: Grant
    Filed: August 3, 2017
    Date of Patent: June 9, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ronald Lebofsky, Jeremy Agresti
  • Patent number: 10676502
    Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution.
    Type: Grant
    Filed: January 24, 2018
    Date of Patent: June 9, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Larry J. Cummings, Mark A. Snyder
  • Patent number: 10676778
    Abstract: Methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays, using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.
    Type: Grant
    Filed: November 20, 2017
    Date of Patent: June 9, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Adam Lowe, Geoff McDermott, Christopher M. Hindson, Erin R. Chia, Amy L. Hiddessen, Benjamin J. Hindson, Chunxiao Han, Yaqi Wang
  • Patent number: 10677693
    Abstract: System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.
    Type: Grant
    Filed: December 28, 2018
    Date of Patent: June 9, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Benjamin J. Hindson, Billy W. Colston, Jr., Kevin D. Ness, Donald A. Masquelier