Patents Assigned to Bio-Rad Laboratories, Inc.
  • DROPLET-BASED ASSAY SYSTEM
    Publication number: 20210220829
    Abstract: System for performing a flow-based assay. The system may comprise a droplet generator to produce an emulsion including droplets in a carrier fluid. The system also may comprise a thermocycler including two or more temperature-controlled zones and also including a channel connected to the droplet generator for receiving the emulsion. The channel may form a single-pass continuous fluid route traversing the temperature-controlled zones multiple times, such that droplets passing through the channel are thermally cycled. The system further may comprise a detection station downstream from the thermocycler and configured to detect a signal from the droplets after such droplets have been thermally cycled by passing through the channel.
    Type: Application
    Filed: December 29, 2020
    Publication date: July 22, 2021
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: Kevin D. NESS, Donald A. MASQUELIER, Billy W. COLSTON, JR., Benjamin J. HINDSON
  • Optical detection system with light sampling
    Patent number: 11067433
    Abstract: System, including methods and apparatus, for optical detection. The system may comprise a light source to generate a beam of light, an optical element including a light guide and an exit window, and at least one detector.
    Type: Grant
    Filed: May 6, 2019
    Date of Patent: July 20, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Denis Pristinski
  • Small nucleic acid quantification using split cycle amplification
    Patent number: 11066698
    Abstract: Methods of detecting or quantifying short RNA or DNA molecules using split cycle amplification are provided.
    Type: Grant
    Filed: February 17, 2016
    Date of Patent: July 20, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Dianna Maar, Samantha Cooper, Wei Yang
  • Emulsion chemistry for encapsulated droplets
    Patent number: 11060136
    Abstract: System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.
    Type: Grant
    Filed: June 20, 2019
    Date of Patent: July 13, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Amy L. Hiddessen, Benjamin J. Hindson
  • Systems and methods for sequencing in emulsion based microfluidics
    Patent number: 11053541
    Abstract: Methods, libraries, and kits for nucleotide sequencing are provided. For example, methods can comprise providing a plurality of partitions, the partitions comprising a set of two or more different primers and a target nucleic acid, wherein different partitions contain different sets of primers with a majority of primers in the sets being common with a different primer set but wherein any two different partitions having a common primer contain no more than one common primer; and hybridizing the target nucleic acid to the primers in the reaction partitions under conditions in which fully complementary primers hybridize to the target sequence and primers that are not fully complementary do not hybridize to the target nucleic acid.
    Type: Grant
    Filed: September 8, 2017
    Date of Patent: July 6, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: John Healy
  • System and method for isolating and analyzing cells
    Patent number: 11052396
    Abstract: A system and method for isolating and analyzing single cells, comprising: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.
    Type: Grant
    Filed: November 4, 2019
    Date of Patent: July 6, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, Priyadarshini Gogoi, Saedeh Javdani Sepehri, Kyle Gleason
  • Microfluidic system and method for arranging objects
    Patent number: 11045805
    Abstract: Microfluidic systems and methods for arranging a set of objects. In an exemplary method, the set of objects may be transported in carrier fluid along a microfluidic channel structure having a reformatting zone including an object-accessible region and at least one object-excluding region. A portion of the carrier fluid may be moved from the object-accessible region to the at least one object-excluding region in an upstream section of the reformatting zone, to reduce a spacing of objects of the set. The portion of the carrier fluid may be directed into the object-accessible region from the at least one object-excluding region in a downstream section of the reformatting zone, to increase a spacing of objects of the set. The steps of moving and directing in combination may increase the spacing between objects disproportionately for a subset of the objects that are closest to one another.
    Type: Grant
    Filed: November 1, 2018
    Date of Patent: June 29, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Lung-Hsin Hung, Robert Meltzer, Lucas Frenz
  • Method of reading the result of an electrophoretic assay comprising a digital image indicating the intensity of light emitted by chemiluminescence from the output medium of the electrophoretic assay
    Patent number: 11035722
    Abstract: A combination imaging system includes a housing having a base and a lid, the lid having a closed position against the base and having an open position. The imaging device further includes a contact area image sensor. The lid shields the contact area image sensor from ambient light when the lid is in the closed position. The imaging device also includes a camera. The camera includes a lens, and the field of view of the camera encompasses at least a portion of an imaging area of the contact area image sensor when the lid is in the open position. The device may be especially useful for capturing a chemiluminescent image of an electrophoretic assay result, and capturing a colorimetric image of the same result, so that non-chemiluminescent protein standards may be located with respect to chemiluminescent analytes of interest.
    Type: Grant
    Filed: December 13, 2018
    Date of Patent: June 15, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Itay Barak
  • METHOD OF PERFORMING DROPLET-BASED ASSAYS
    Publication number: 20210170412
    Abstract: Method of analysis. In the method, a first emulsion and a second emulsion substantially separated from one another by a spacer fluid may be formed. The first emulsion, the spacer fluid, and the second emulsion may be flowed in a channel from a fluid inlet to a fluid outlet of a heating and cooling station having two or more temperature-controlled zones, such that each emulsion is thermally cycled to promote amplification of a nucleic acid target in droplets of the emulsion. Amplification data may be collected from individual droplets of each emulsion downstream of the heating and cooling station. A level of the nucleic acid target present in each emulsion may be determined based on the amplification data collected from the individual droplets of the emulsion.
    Type: Application
    Filed: December 2, 2020
    Publication date: June 10, 2021
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: Kevin D. NESS, Donald A. MASQUELIER, Billy W. COLSTON, JR., Benjamin J. HINDSON
  • Dimeric reverse transcriptase
    Patent number: 11021693
    Abstract: Covalently-linked DNA polymerases are provided.
    Type: Grant
    Filed: July 29, 2019
    Date of Patent: June 1, 2021
    Assignee: BIO-RAD LABORATORIES, INC.
    Inventor: Xiao-Song Gong
  • RNA amplification methods
    Patent number: 11021735
    Abstract: Methods of detecting and amplifying short RNAs are provided.
    Type: Grant
    Filed: June 15, 2018
    Date of Patent: June 1, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Man Cheng, Xiao-Song Gong, Yan Wang, Adam M. McCoy
  • Droplet tagging contiguity preserved tagmented DNA
    Patent number: 11021738
    Abstract: A method of maintaining contiguity in chromosomal DNA following treatment with a tagmentase. Conditions are selected such that the tagmentase does not release from the DNA, and thus forms a bridge linking DNA segments that have the same relationship (haplotype) as occurred in the genomic DNA. Thus the tagmentase step can occur in bulk (before partitions are formed). The resulting tagmentase-bridged DNA segments can be added to partitions maintaining the bridged segments until they are introduced into different partitions. Once in partitions, the contiguous DNA segments can be barcoded with a partition-specific barcode, thereby allowing for later identification of contiguous DNA after sequencing in bulk (after partitions contents are merged).
    Type: Grant
    Filed: December 19, 2017
    Date of Patent: June 1, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ronald Lebofsky, Jeremy Agresti
  • Detection system with one-piece optical element to concentrate and homogenize light
    Patent number: 11022785
    Abstract: Detection system comprising an examination region, a one-piece optical element including a focusing portion to concentrate light received from the examination region and a guiding portion to homogenize light received from the focusing portion, and a detector configured to detect homogenized light received from the guiding portion.
    Type: Grant
    Filed: January 16, 2020
    Date of Patent: June 1, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Denis Pristinski, David P. Stumbo
  • METHOD AND SYSTEM FOR SAMPLING MATERIAL FROM CELLS
    Publication number: 20210155975
    Abstract: Methods, systems, and devices for sampling/isolating material from cells. An exemplary system may comprise a chip including an electrode array of sampling electrodes arranged along a surface of the chip. A cell-receiving area may be located adjacent the surface of the chip. The system also may comprise a tag array of tags supported by the chip and aligned with the electrode array. Each tag of the tag array may include an identifier that is unique to the tag within the tag array. Each tag may be configured to bind nucleic acids, or a capturing agent distinct from the tag may be aligned with each sampling electrode of the electrode array to capture a protein or other analyte of interest. The system further may comprise a control circuit configured to apply an individually controllable voltage to each sampling electrode of the electrode array and measure an electrical property of the sampling electrode.
    Type: Application
    Filed: November 25, 2020
    Publication date: May 27, 2021
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: Arkadiusz BIBILLO, Pranav PATEL, Christopher REGGIARDO, Jonathan PETERSEN
  • PARTITION-BASED DETERMINATION OF TARGET COPY NUMBER FOR SINGLE CELLS BY NON-ENDPOINT AMPLIFICATION
    Publication number: 20210147925
    Abstract: Methods of analyzing a sample including cells and/or cell-free nuclei. In an exemplary method, partitions may be formed, with each partition including a portion of the same sample. Each partition of at least a subset of the partitions may contain only one of the cells/nuclei from the sample. Cells and/or cell-free nuclei from the sample may be lysed in the partitions. At least one amplification reaction may be performed for a target or set of targets in the partitions. Amplification data may be collected from the partitions in an exponential/linear phase of each amplification reaction. A copy number of the target or set of targets may be determined for individual partitions using the amplification data, to determine if either a duplication or deletion is present in all or a subset of the cells analyzed.
    Type: Application
    Filed: November 13, 2020
    Publication date: May 20, 2021
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: George KARLIN-NEUMANN, Douglas GREINER
  • Synthesizing barcoding sequences utilizing phase-shift blocks and uses thereof
    Patent number: 10988808
    Abstract: Provided herein are compositions and methods for generating phase-shift barcode oligonucleotides for library construction for next-generation sequencing. In some cases, barcode oligonucleotides are attached to particles or beads. Also provided are methods and kits for using the phase-shift barcode oligonucleotides in sequencing assays.
    Type: Grant
    Filed: February 26, 2020
    Date of Patent: April 27, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Lucas Frenz, Jeremy Agresti
  • Reverse transcriptases and uses thereof
    Patent number: 10988762
    Abstract: Hybrid reverse transcriptases formed from portions of FLVRT and MLVRT are provided.
    Type: Grant
    Filed: February 13, 2018
    Date of Patent: April 27, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Xiao-Song Gong, Yan Wang
  • System and method for capturing and analyzing cells
    Patent number: 10975422
    Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.
    Type: Grant
    Filed: July 30, 2018
    Date of Patent: April 13, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, Priyadarshini Gogoi, Saedeh Sepehri Javdani, Yi Zhou
  • Mutant DNA polymerases
    Patent number: 10961518
    Abstract: Mutant Type-A DNA polymerases having increased resistance to one or more polymerization activity inhibitors are provided.
    Type: Grant
    Filed: January 15, 2019
    Date of Patent: March 30, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Xiangdong Meng, Man Cheng
  • Droplet velocity detection
    Patent number: 10962563
    Abstract: Methods and systems are provided for measuring a velocity of a droplet passing through a microfluidic channel.
    Type: Grant
    Filed: January 11, 2019
    Date of Patent: March 30, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Aaron Weber, Tony Hung, Sepehr Kiani