Abstract: The present invention includes a method and apparatus for the detection of a target material. The method and apparatus includes providing a substrate with a surface and forming a domains of deposited materials thereon. The deposited material can be placed on the surface and bound directly and non-specifically to the surface, or it may be specifically or non-specifically bound to the surface. The deposited material has an affinity for a specific target material. The domains thus created are termed affinity domains or deposition domains. Multiple affinity domains of deposited materials can be deposited on a single surface, creating a plurality of specific binding affinity domains for a plurality of target materials. Target materials may include, for example, pathogens or pathogenic markers such as viruses, bacteria, bacterial spores, parasites, prions, fungi, mold or pollen spores.
Type:
Grant
Filed:
May 30, 2002
Date of Patent:
May 24, 2005
Assignee:
BioForce Nanosciences, Inc.
Inventors:
Eric R. Henderson, Saju R. Nettikadan, Curtis L. Mosher
Abstract: The present invention is a method for selectively removing objects from a surface utilizing a probe. The probe is scanned over the surface utilizing a greater and greater relative amount of force so that a certain number of the objects are removed from the surface. The force required to remove the objects from the surface can be calculated utilizing Hook's law and the spring constant of the probe. After removal of the objects that have a relatively weaker binding affinity with the surface, the remaining objects can be harvested, characterized, and subjected to further study.
Abstract: The invention is a solid state process for analyzing genomes by visualizing sequence specific markers (e.g., proteins that bind a defined DNA sequence elements) by scanning probe microscopy. The method includes linear display of the nucleic acid on a solid surface, image acquisition by the scanning probe microscope, and digital data analysis. The acts of the method result in a bar code type display of each fragment of the DNA sample. These bar codes are then used to place the fragments in the order they appear on the original DNA sample.
Abstract: Provided are methods of detecting molecular interactions using arrays and near field scanning probe techniques. Also provided are methods of characterizing binding interactions under defined reaction parameters, methods of determining antibody binding specificity, methods of selecting a substrate for an array of immobilized molecules and methods of determining molecular occupancy time with respect to binding interactions.
Type:
Application
Filed:
April 30, 2003
Publication date:
October 2, 2003
Applicant:
BioForce Nanosciences, Inc.
Inventors:
Eric Henderson, James Johnson, Saju Nettikadan
Abstract: The invention is a method for the formation and analysis of novel miniature deposition domains. These deposition domains are placed on a surface to form a molecular array. The molecular array is scanned with an AFM to analyze molecular recognition events and the effect of introduced agents on defined molecular interactions. This approach can be carried out in a high throughput format, allowing rapid screening of thousands of molecular species in a solid state array. The procedures described here have the added benefit of allowing the measurement of changes in molecular binding events resulting from changes in the analysis environment or introduction of additional effector molecules to the assay system. The processes described herein are extremely useful in the search for compounds such as new drugs for treatment of undesirable physiological conditions.
Type:
Grant
Filed:
May 18, 2000
Date of Patent:
June 3, 2003
Assignee:
BioForce Nanosciences, Inc.
Inventors:
Eric Henderson, Curtis Mosher, Michael P. Lynch
Abstract: An apparatus for the stack formation is described, by means of which a practically continuous individual stack formation is possible from a stream of slices produced by means of a cutting apparatus. For this purpose a partial stack is formed by means of a stack receiver, and is transferred for the further stack formation to a portioning band, so that, during the continuation of the stack formation on the portioning band, a return guidance of the stack receiver can take place into a position from which it can be shot anew into the stream of slices for the formation of a next partial stack.