Abstract: Systems and methods for calibration of retinal prosthetics in accordance with embodiments of the invention are illustrated. A closed loop calibration process is described whereby a multi-electrode stimulation regime can be calibrated to a given user's retina. Multi-electrode stimulation can provide increased stimulation selectivity, but significantly increases complexity. Systems and methods described herein provide computational steps that significantly reduce the amount of trials and computation required in order to achieve clinically viable selectivity, making closed-loop calibration of retinal prosthetics possible in significantly less time than open-loop calibration.

Abstract: A dual ring resonator including a base substrate, and a silicon oxide layer positioned on the base substrate having a first waveguide, a primary ring resonator optically coupled to the first waveguide, a second waveguide, and a secondary ring resonator optically coupled to the primary ring resonator and the second waveguide. The dual ring resonator further includes at least one heater disposed partially on the primary ring resonator, and a trench within at least the silicon oxide layer and surrounding at least a portion of the first waveguide and the second waveguide.

Abstract: Methods and formulations of treatment for menstrual complications, gestational complications, and to prolong gestation are described. Treatments include administration of a compound related to regulation of gestational progress or uterine contractions.

Abstract: Chronic non-healing wounds represent a major source of morbidity for patients, and a significant economic burden. Current wound care treatments are passive and are unable to adapt to changes in the wound environment in real time. By integrating multimodal sensors and adding stimulators in a bandage, physiological monitoring is possible and provides an opportunity for active intervention into the complex wound environment. Here, we develop a battery-free flexible bioelectronic system consisting of wirelessly powered, closed-loop, autonomous sensing and stimulation circuits with tissue-interfacing tough conducting hydrogel electrodes for robust signal transduction, on-demand adhesion, and detachment.

Abstract: A retina clip in accordance with embodiments of the invention are illustrated. One embodiment includes a retina clip including an outer ring having a means of retention against the retina, and an inner platform configured to hold a payload against the retina of an eye, where the inner platform is suspended from the inner ring via several springs. In many embodiments, the springs are flexures. The springs can be modified to generate a desired deflection based on a given payload attached to the inner platform. In numerous embodiments, the payload is a microelectrode array used as part of a retinal prosthetic.

Abstract: Embodiments herein describe systems and methods to determine nucleic acid thermodynamics and uses thereof. Many embodiments utilize a sequencing chip, such as an Illumina flow cell as a high-throughput platform for performing massively parallel melt curve determination. In many embodiments, nucleic acid molecules possessing a region that forms a secondary structure are affixed to a sequencing chip and hybridized with one or more labeled oligonucleotides. As the secondary structure denatures, changes in fluorescence can be measured to determine a melt curve of specific sequences.

Abstract: A device for manipulating tissue at a vessel includes an elongated member having a proximal end and a distal end, a guide member at the distal end of the elongated member, the guide member having a blunt distal tip for engagement against an interior wall of the vessel, and a tissue cutting device at the distal end of the elongated member, wherein the tissue cutting device has a sharp tip that is proximal to the blunt distal tip of the guide member.

Abstract: Systems and methods for predicting and treating relapses for neurological conditions in accordance with embodiments of the invention are illustrated. One embodiment includes a method for predicting and treating a clinical neurological condition relapse. The method includes steps for selecting a threshold heart rate variability value for a patient suffering from a clinical neurological condition, monitoring, using a cardiac monitor, the heart rate variability of the patient over time, providing an indicator that a relapse is imminent when the heart rate variability of the patient falls below the threshold heart rate variability value, and treating the patient using a transcranial magnetic stimulation device by applying an accelerated theta burst stimulation protocol where the transcranial magnetic stimulation target is the left prefrontal dorsolateral cortex.

Abstract: A method for ultrasound power Doppler image reconstruction includes obtaining sparse sequences of compound frames of ultrasound data; inputting the sparse sequences into a convolutional neural network; and generating as output from the convolutional neural network a reconstructed time series of power Doppler images corresponding to the sparse sequences. The convolutional neural network is trained using a custom loss function to learn a reconstruction function using ground truth images from high-quality in vivo images.

Abstract: Provided herein are, for example, systems and methods of diagnosing and treating depression, in which a transcranial magnetic stimulation (TMS) therapy is administered to a subject in need thereof and measuring a TMS evoked response in the subject, are provided. The systems and methods allow tailoring or optimizing of a treatment protocol for maximal individual benefit, thereby providing an individualized and optimized treatment protocol for psychiatric disorders such as depression.

Abstract: A laser beam steering system is disclosed which includes a laser source which produces a pulsed laser light beam with a frequency comb spectrum, a first metasurface configured to i) directly receive the pulsed laser and ii) directly generate a diffracted pulsed laser output at different frequencies with a beam at a center frequency normal to the first metasurface; and a second metasurface configured to i) directly receive the diffracted pulsed laser output and ii) to focus onto different foci at a focal plane, light propagating from the focal plane leads to generation of one or more optical beams that are controlled in space and time.

Abstract: Activity-based probe compounds for use in labeling a cysteine protease are provided. The compounds are targeted to the protease through a specific targeting element. The compounds additionally include a detectable element, such as a fluorescent label, a radiolabel, or a chelator. In some cases, the compounds additionally include a quenching element that is released upon reaction with the protease. Also provided are compositions comprising the compounds and methods for using the compounds, for example in labeling a protease in an animal and in visualizing a tumor in an animal.

Abstract: Systems and methods for deep brain stimulation using kinematic feedback in accordance with embodiments of the invention are illustrated. One embodiment includes a deep brain stimulation system, including an implantable neurostimulator, a first inertial measurement unit (IMU), a second IMU, and a controller, where the controller is communicatively coupled to the implantable neurostimulator, the first IMU, and the second IMU, and where the controller is configured to obtain kinematic data from the first IMU and the second IMU, identify an abnormal movement event based on the kinematic data, and modify deep brain stimulation provided by the implantable neurostimulator based on the identified abnormal movement event.

Abstract: Engineered promiscuous biotin ligases and methods of using them in proximity labeling are described. In particular, the invention provides novel biotin ligase variants having increased promiscuous biotinylation activity capable of proximity labeling of proteins in live cells in as little as 10 minutes.

Abstract: Functional human cortico-spinal-muscle assembled spheroids are generated by in vitro culture. Complete cortico-spinal-muscle spheroids (hCS-hSC-hSkM) are assembled from component cultured cell systems, where each cultured cell system is designed to provide specific sets of neural and/or muscle cells, and which components are functionally integrated in the assembled spheroid.

Abstract: Methods are provided for the cell-based delivery of collagen VII for the treatment of Epidermolysis Bullosa and corneal erosion. The disclosure also provides a composition and a pharmaceutical composition comprises, comprise, or alternatively consist essentially of, or yet further consist of a keratinocyte sheet or a corneal cell sheet.

Abstract: Methods, systems, compositions and strategies for the delivery of WW domain-containing fusion proteins into cells in vivo, ex vivo, or in vitro via ARMMs are provided. Methods, systems, compositions and strategies for the delivery of Cas9 proteins and/or Cas9 variants into cells in vivo, ex vivo, or in vitro via fusion to ARMM associated proteins (e.g., ARRDC1 or TSG101) are also provided.

Abstract: A method is provided for detecting one or more analytes in a sample. The method relies, in part, on the ability of functionalized particles added to the sample to partially or completely inhibit the transmission of electromagnetic radiation into and out of the sample through a detection surface in a reaction vessel containing the sample. In a microarray format, the invention can be used to screen millions, billions or more biological elements, such as an organism, cell, protein, nucleic acid, lipid, saccharide, metabolite, or small molecules. Methods, apparatuses and kits are described.

Abstract: Compositions and methods for treating hyperinsulinemic hypoglycemia, such as hyperinsulinemic hypoglycemia after bariatric surgery, are provided. In some embodiments, an effective amount of the glucagon-like peptide-1 receptor antagonist exendin(9-39) is subcutaneously administered twice per day.

Abstract: A device for positioning an electrode in tissue includes: a lead body having a distal portion; an electrode array coupled to the lead distal portion; an anchoring element having an anchor tip and being operable in a first configuration in which the anchor tip is retracted within the lead and in a second configuration in which the anchor tip is extended outside the lead and configured to fixate within the tissue; and a displacement mechanism that is actuated to bias the electrode array or the anchoring element toward the tissue. A method for positioning an electrode in tissue includes: navigating, to the tissue, a lead with an electrode array, an anchoring element with a distal anchor tip, and a displacement mechanism; biasing the electrode array and anchoring element towards the tissue with the displacement mechanism; and deploying the anchoring element, and verifying fixation of the anchor tip within the tissue.