Abstract: The present invention provides a eukaryotic expression vector containing a gene to be expressed and a TATA box, whereby, 1-200 bp base pair (bp) upstream (5') of the TATA box, there is a first multimeric enhancer segment and 1-100 bp below (3') the gene to be expressed there is provided a second multimeric enhancer segment. The present invention also provides a process for the preparation of this expression vector. Furthermore, the present invention is concerned with the use of this expression vector.

Abstract: The present invention concerns compounds of the formula: ##STR1## where R.sub.1 is an alkyl or alkenyl radical containing up to 6 carbon atoms, a cycloalkyl radical containing 3 to 7 carbon atoms, an aralkyl, aralkenyl or aryl radical in which the aryl radical or moiety can be substituted one or more times by halogen, C.sub.1 -C.sub.6 -alkyl, C.sub.1 -C.sub.6 -alkoxy, hydroxyl, trifluoromethyl, cyano, nitro, amino, C.sub.1 -C.sub.6 -alkylamino, C.sub.2 -C.sub.12 -dialkylamino, C.sub.1 -C.sub.6 -acylamino, C.sub.1 -C.sub.16 -acyl, C.sub.1 -C.sub.6 -alkylsulphenyl, -sulphinyl or -sulphonyl or by azido, R.sub.2 is hydrogen atom or a C.sub.1 -C.sub.6 -alkyl, aralkyl, aralkenyl or acyl radical, A and B are saturated or unsaturated alkylene chains containing up to 10 carbon atoms which can be substituted one or more times by C.sub.1 -C.sub.

Abstract: The present invention provides a carrier for coating with immunologically-active material, wherein it consists of an injection molded synthetic resin with a content of adjuvant and additional materials of less than 1% by weight. The present invention also provides a carrier coated with immunologically-active material, wherein the carrier consists of synthetic resin with a content of adjuvant and additional materials of less than 1% by weight.

Abstract: The present invention provides a restriction endonuclease which recognizes palindromic sequences ##STR1## where C* is methylated, and cleaves these sequences at the position indicated by the arrows. This endonuclease is preferably from a microorganism of the genus Kluyvera. The present invention also provides a process for obtaining this new restriction endonuclease and a method for using the endonuclease.

Abstract: The invention comprises DNA expressing a Bacillus thuringiensis protein toxic to Coleoptera, as well as the gene which expresses the protein. Also included are plasmids which contain this DNA and non-Bacillus thuringiensis bacterium transformed by the DNA, the plasmid, or the gene.

Abstract: The invention relates to a composition useful in determining an analyte in a sample. The composition contains an indicator system which forms a detectable signal in the presence of said analyte, and which is not a coupling system, as well as a pyrogallol derivative. The pyrogallol derivative causes uniform formation of detectable signal. Also described are kits useful in determining an analyte, apparatus which incorporate the composition, and a method for determining an analyte using the composition.

Abstract: The present invention provides diphosphonic acid derivatives of the general formula: ##STR1## according to claim 1. These compounds are useful for the treatment or prophylaxis of calcium metabolism disturbance or disease.

Abstract: The present invention provides a carrier band which is movable stepwise in its longitudinal direction past an optical measurement device and on its side facing the measurement device carries test strips to be impregnated with a test liquid running transversely to its longitudinal direction, wherein the test strips are fixed on the carrier band at equal distances to one another at least next to the edges of the carrier band. The present invention also provides a device for fixing test strips on to a carrier band.

Abstract: The present invention provides a restriction endonuclease, characterized by the recognition sequence:5'-CTCTTCN.dwnarw.NNN-N-3'3'-GAGAAGN-NNN.uparw.N-5'and the cleavage position defined by the arrows. The present invention also provides a process for obtaining this new restriction endonuclease and a method for using the endonuclease.

Abstract: The present invention provides compounds of the general formula: ##STR1## wherein E is an alkylene or alkenylene radical containing 2 carbon atoms, R.sup.1 is a cycloaliphatic hydrocarbon radical containing 5 to 12 carbon atoms, which is optionally substituted by up to 4 lower alkyl radicals, as well as halogen atoms, and can optionally be mono- or polyunsaturated, R.sup.2 and R.sup.3, which can be the same or different, are further cycloaliphatic hydrocarbon radicals containing 3 to 7 carbon atoms, hydrogen or halogen atoms, trifluoromethyl radicals or alkyl or alkoxy radicals containing up to 7 carbon atoms which can optionally also be substituted with methyl radicals, X is hydroxyl, Y is a residue of an alcohol or a residue of an amine or the group OZ, Z is hydrogen, an alkali metal or ammonium ion or X and Y taken together are --O--. Thus, these compounds are lactones or hydroxy acids of the formulae: ##STR2## wherein E, R.sup.1, R.sup.2 and R.sup.

Abstract: The present invention provides a process for the determination of a bindable analyte according to the principle of heterogeneous immunoassay by incubation of a sample solution which contains the analyte with a labelled first receptor specifically bindable with the analyte and present in dissolved phase and a second receptor present in a solid phase which does not cross-react with the first receptor and can fix a complex which contains the analyte and first receptor, separation of the phases after incubation and quantitative measurement of the labelling bound to the solid phase, wherein there is determined the back dissociation velocity of the labelling bound to the solid phase into the dissolved phase and the quotients of the back dissociation velocity and measurement value are used as a measure for the correctness of the test result of the first measurement.

Abstract: A transmission for converting rotary motion to simultaneous reciprocating motion of a tool in two mutually transverse directions. A driver is selectively attached to one end of an input shaft and the opposite end of the input shaft terminates at an eccentric cam. An output shaft extends between a driven end and a working end. The driven end is apertured to rotatably receive the eccentric cam and the output shaft is slidably received in the diametrically extending bore of a spherical bearing member. Upon rotation of the input shaft, the working end of the output shaft is caused to reciprocate toward and away from the input shaft and, simultaneously, laterally relative to a longitudinal axis of the input shaft. The working end of the output shaft may be provided with appropriate cutting teeth to enable its use, for example, as a power broach for the precise removal of bone to enable proper implantation of a prosthetic device.

Abstract: The invention provides new redox indicators and uses for these redox compounds (I): ##STR1## Wherein X is oxygen or sulphur, R.sub.1 is julolidine or tetrahydroquinoline, which can carry an alkyl radical on the nitrogen atom which, in turn, can be substituted by a sulphuric, phosphonic or carboxylic acid residues, or is (II); wherein R.sub.4 is hydroxyl or a mono- or dialkylated amino group, R.sub.5 and R.sub.6, which can be the same or different, are hydrogen, alkyl or alkoxy, R.sub.2 is hydrogen or alkyl, julolidine or tetrahydroquinoline, which can carry an alkyl on the nitrogen atom which alkyl can be substituted by sulphuric, phosphonic or carboxylic acids, or is III; wherein R.sub.5 and R.sub.6 are the same as in R.sub.1 and R'.sub.4 is hydroxyl, an amino or a mono-or dialkylated amino group, whereby the alkyl radicals can be substituted one or more times by hydroxyl, alkoxy, halogen, morpholine or a sulphuric, carboxylic or phosphonic acid residue, which acids can also be esterified, and R.sub.

Abstract: Improved resistance to linear displacement through bone is observed in a prosthetic patellar joint when the joint is attached to the patella by a protuberance which has a concave shape.

Abstract: The present invention provides a recombinant DNA, wherein it contains the consensus sequence: ##STR1## and a promoter which has the DNA sequence GGN.sub.10 GC. The present invention also provides a process for the isolation of this recombinant DNA, wherein a DNA sequence containing the consensus sequence is identified from a gene bank of a micro-organism, which contains a gene which is repressible by oxygen and inducible by formate under anaerobic conditions, isolated and combined with an appropriate promotor according to known methods.This recombinant gene can be used for the inducible and repressible expression of a foreign gene by bringing about the induction by formate under anaerobic conditions and the repression by oxygen.

Abstract: A regulated power supply employs two separate sources of power which are serially connected by a variable impedance element, such as a transistor, connected between the two power sources. Output voltage of the power supply is equal to the sum of the individual power supply voltages minus a voltage drop across the variable impedance element. A sensor circuit employing standby and active mode branches is coupled between output terminals of the power supply for control of the variable impedance element. The active branch of the sensor is switchably connected to an output power terminal of the supply so as to be operative only during the active mode. The standby branch comprises a resistive network coupled to a reference diode while the active branch comprises a feedback amplifier coupled to the reference diode.

Abstract: The present invention provides an enzymatically-inactive, immunologically-active .beta.-galactosidase mutein, wherein, in the region between the amino acids 430 and 550, at least one amino acid of the natural sequence is changed to another amino acid and the enzymatic activity does not amount to more than 1%, referred to the native enzyme. The present invention also provides a process for the production of this mutein. Furthermore, the present invention is concerned with the use of this mutein in the immunological determination of serum proteins by the enzyme immunoassay principle.

Abstract: The new pyrrolobenzimidazoles according to the present invention are compounds of the general formula: ##STR1## wherein R.sub.1 is a hydrogen atom or an alkyl, alkenyl or cycloalkyl radical, R.sub.2 is a hydrogen atom, an alkyl or alkenyl radical or a cyano group or a carbonyl group substituted by a hydroxyl or hydrazino group or by an alkyl, alkoxy, amino, alkylamino or dialkylamino group or together with R.sub.1 represents a cycloalkylene radical or R.sub.1 and R.sub.2 together form an alkylidene or cycloalkylidene radical, X is a valency bond, a C.sub.1 -C.sub.4 alkylene radical or a vinylene radical, T is an oxygen or sulphur atom and Py is a 2-, 3- or 4-pyridyl radical which optionally carries an oxygen atom on the ring heteroatom and/or can be substituted one or more times by alkyl, alkoxy, hydroxyl, cyano or nitro, as well as by halogen; the tautomers thereof and the physiologically acceptable salts thereof with inorganic and organic acids.

Abstract: The present invention provides a process for the determination of iron in body fluids by liberation of the bound iron, reduction to Fe.sup.2+, addition of a color system appropriate for the detection of iron and photometric measurement in a tenside-containing sample solution, wherein a fatty acid polyethylene glycol ester, an alkanol polyglycol ether and at least 1 mole/liter quanidine hydrochloride are added to the sample solution. The present invention also provides a reagent for the determination of iron in serum, containing a reducing agent, a color material system appropriate for the detection of iron and guanidine hydrochloride and at least one tenside, wherein it contains a fatty acid polyethylene glycol ester and a alkanol polyglycol ether.

Abstract: A dynamic bone fixation screw for rejoining fracture fragments of a bone to their approximate original mutual dispositions. It includes a threaded distal end member for threaded engagement with one of the bone fragments and a head member at a proximal end for engagement with another of the bone fragments. A spring member integral with and extending between the distal end member and the head member enables a predetermined torque to be initially imparted to the bone. When stress relaxation in the bone occurs, the spring member relaxes accordingly while continuing to draw the bone fragments together. The bone screw is fabricated in a unique manner.