Patents Assigned to Caliper Life Sciences, Inc.
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Publication number: 20160377562Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.Type: ApplicationFiled: June 27, 2016Publication date: December 29, 2016Applicants: CALIPER LIFE SCIENCES, INC., CANON U.S. LIFE SCIENCES, INC.Inventors: Steven A. Sundberg, Michael R. Knapp, Ivor T. Knight, Deborah J. Boles, Aaron Rulison, Wesley B. Dong, Andrew Fabans, Allen Boronkay, Edwawrd Donlon, Robert Moti, Michael Slater
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Patent number: 9446151Abstract: Compositions and methods are described for detecting enzyme activity in a live organism (e.g., animal) are provided.Type: GrantFiled: April 13, 2012Date of Patent: September 20, 2016Assignee: Caliper Life Sciences, Inc.Inventors: Daniel Ansaldi, Kevin P. Francis, Rajendra Singh, Ning Zhang
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Patent number: 9420148Abstract: The invention provides a method and apparatus for isolating individual target cells. The apparatus includes a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction. A plurality of trapping channels intersect the collection channel, each trapping channel having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell. The apparatus also includes an imaging system configured to image individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyses of the imaging data; and a directing system configured to direct the individual target cells. A pressure source is in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.Type: GrantFiled: October 19, 2015Date of Patent: August 16, 2016Assignee: CALIPER LIFE SCIENCES, INC.Inventors: Rajiv Bharadwaj, Bahram Fathollahi
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Patent number: 9376718Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.Type: GrantFiled: April 11, 2012Date of Patent: June 28, 2016Assignees: Caliper Life Sciences, Inc., Canon U.S. Life Sciences, Inc.Inventors: Steven A. Sundberg, Michael R. Knapp, Ivor T. Knight, Deborah J. Boles, Aaron J. Rulison, Wesley B. Dong, Edward P. Donlon, Robert J. Moti, Andrew G. Fabans, Allen R. Boronkay, Michael Slater
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Patent number: 9314218Abstract: An integrated microtomography and optical imaging system includes a rotating table that supports an imaging object, an optical stage, and separate optical and microtomography imaging systems. The table rotates the imaging object about a vertical axis running therethrough to a plurality of different rotational positions during a combined microtomography and optical imaging process. The optical stage can be a trans-illumination, epi-illumination or bioluminescent stage. The optical imaging system includes a camera positioned vertically above the imaging object. The microtomography system includes an x-ray source positioned horizontally with respect to the imaging object. Optical and x-ray images are both obtained while the imaging object remains in place on the rotating table. The stage and table are included within an imaging chamber, and all components are included within a portable cabinet.Type: GrantFiled: June 20, 2011Date of Patent: April 19, 2016Assignee: Caliper Life Sciences, Inc.Inventors: Daniel G. Stearns, David G. Nilson, Bradley W. Rice
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Patent number: 9297784Abstract: The invention provides devices, systems, and methods for extracting target objects from a sample. In the method, a stream of sample containing a plurality of target and non-target objects is directed by first and second streams of buffer through a sample inlet channel into a fluid junction and through the fluid junction into a sample waste channel. In response to detecting a target object within the stream of sample, an actuator is energized to close a normally open valve, resulting in a transient burst of cross-flow into the fluid junction that briefly diverts the flow of sample within the fluid junction and results in an aliquot of sample being directed into an aliquot delivery channel. The combination of the valve and the actuator acts as a self-limiting pulse generator.Type: GrantFiled: March 15, 2013Date of Patent: March 29, 2016Assignee: CALIPER LIFE SCIENCES, INC.Inventors: Joshua I. Molho, Daniel G. Stearns, I-Jane Chen, Danh Tran, Bradley W. Rice, Tobias Daniel Wheeler, Alexander V. Dukhovny
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Patent number: 9278354Abstract: The invention provides methods of controlling environmental conditions within a fluidic system, where such environmental conditions can affect the operation of the system in its desired function, and fluidic channels, devices, and systems that are used in practicing these methods. Such methods are generally directed to environmental control fluids, the movement of such fluids through these systems, and the interaction of these fluids with other components of the system, e.g., other fluids or solid components of the system.Type: GrantFiled: March 17, 2015Date of Patent: March 8, 2016Assignee: CALIPER LIFE SCIENCES, INC.Inventors: J. Wallace Parce, Yung-Mae M. Yao, Donald J Morrissey
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Publication number: 20160044212Abstract: The invention provides a method and apparatus for isolating individual target cells. The apparatus includes a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction. A plurality of trapping channels intersect the collection channel, each trapping channel having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell. The apparatus also includes an imaging system configured to image individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyses of the imaging data; and a directing system configured to direct the individual target cells. A pressure source is in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.Type: ApplicationFiled: October 19, 2015Publication date: February 11, 2016Applicant: Caliper Life Sciences, Inc.Inventors: Rajiv Bharadwaj, Bahram Fathollahi
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Patent number: 9207249Abstract: The present invention is an automated microfluidic chip processing apparatus that includes a deck for holding at least one microfluidic chip and capable of being accessed by a liquid handling system, a fluid control system, and a detection system, wherein a chip handling device transports the chip from the deck to the fluid control system and the detection system. The present invention also includes a chip for use with an automated microfluidic chip processing apparatus, and a method for processing a microfluidic chip using such an apparatus.Type: GrantFiled: July 29, 2013Date of Patent: December 8, 2015Assignee: Caliper Life Sciences, Inc.Inventors: Michael Greenstein, Colin B Kennedy, James C Mikkelsen, Jr.
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Patent number: 9149466Abstract: Dopamine reuptake inhibitors, and their analogs, are disclosed for treating and delaying the progression of autoimmune diseases.Type: GrantFiled: March 7, 2014Date of Patent: October 6, 2015Assignee: Caliper Life Sciences, Inc.Inventors: Hao Chen, Alexei Miagkov, Lisa Leary, Ming Liu, Qi Su
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Patent number: 9119843Abstract: Method of using dopamine reuptake inhibitors, e.g., sydnonimine derivatives, for the management of diabetic symptoms and associated complications or conditions, such as hyperglycemia and diabetic neuropathy.Type: GrantFiled: December 3, 2010Date of Patent: September 1, 2015Assignee: Caliper Life Sciences, Inc.Inventors: Hao Chen, Ming Liu, Malathi Sathyamoorthy, Qi Su, Lisa Leary, Wayne Shaobin Zhong
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Patent number: 9101928Abstract: An array of transportable particle sets is used in a microfluidic device for performing chemical reactions in the microfluidic device. The microfluidic device comprises a main channel and intersecting side channels, the main channel and side channels forming a plurality of intersections. The array of particle sets is disposed in the main channel, and the side channels are coupled to reagents. As the particle sets are transported through the intersections of the main channel and the side channels, reagents are flowed through the side channels into contact with each array member (or selected array members), thereby providing a plurality of chemical reactions in the microfluidic system.Type: GrantFiled: January 27, 2011Date of Patent: August 11, 2015Assignee: Caliper Life Sciences, Inc.Inventors: Tammy Burd Mehta, Anne R. Kopf-Sill, J. Wallace Parce, Andrea W. Chow, Luc J. Bousse, Michael R. Knapp, Theo T. Nikiforov, Steve Gallagher
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Patent number: 9097723Abstract: The invention provides methods and apparatuses that allow a protein sample to undergo reduction, alkylation, and digestion in a continuous flow process carried out within a microfluidic device. Methods and apparatuses in accordance with the invention can be employed as part of an automated proteomics analysis carried out in an integrated proteomics system.Type: GrantFiled: April 3, 2006Date of Patent: August 4, 2015Assignee: Caliper Life Sciences, Inc.Inventors: Bahram Fathollahi, Javier A. Farinas, Andrea W. Chow, Stephane Mouradian
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Publication number: 20150198593Abstract: A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip; electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region; applying a magnetic field to the magnetic holding region to fix the protein-attached beads in place within the magnetic holding region; binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.Type: ApplicationFiled: January 15, 2015Publication date: July 16, 2015Applicant: CALIPER LIFE SCIENCES, INC.Inventors: Andrew Barry, Laurel Provencher, Seth Cohen, I-Jane Chen, Jun Yan, Jingjing Wang
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Publication number: 20150193928Abstract: The invention provides a method and apparatus for isolating individual target cells. The apparatus includes a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction. A plurality of trapping channels intersect the collection channel, each trapping channel having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell. The apparatus also includes an imaging system configured to image individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyses of the imaging data; and a directing system configured to direct the individual target cells. A pressure source is in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.Type: ApplicationFiled: January 13, 2015Publication date: July 9, 2015Applicant: CALIPER LIFE SCIENCES, INCInventors: Rajiv Bharadwaj, Bahram Fathollahi
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Patent number: 9034196Abstract: A microfluidic device with a filter includes a substrate; a flowpath including a well formed in the substrate in fluid communication with a channel formed in the substrate; and a filter disposed across the flowpath and associated with the channel.Type: GrantFiled: September 21, 2011Date of Patent: May 19, 2015Assignee: Caliper Life Sciences, Inc.Inventors: Stephane Mouradian, Josh Molho, Ming Wu, Hayley Wu, Javier A. Farinas
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Patent number: 9012206Abstract: The invention provides methods of performing a sizing analysis. In the methods, a sizing ladder used in performing the sizing analysis is corrected. In one method, the sizing ladder is corrected for batch-to-batch variations in a sieving gel. In another method, the sizing ladder is corrected for a sample concentration that is different from the archival sizing ladder concentration. Methods are also provided in which the sizing ladder is corrected using a standard marker in a sample and/or using a real-time standard sizing ladder. The methods may be used individually or in combination.Type: GrantFiled: October 28, 2014Date of Patent: April 21, 2015Assignee: Caliper Life Sciences, Inc.Inventors: Dan Camporese, Advit Bhatt, Josh Molho, Hui Xu, Ken Summers
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Patent number: 8980644Abstract: The invention provides methods of controlling environmental conditions within a fluidic system, where such environmental conditions can affect the operation of the system in its desired function, and fluidic channels, devices, and systems that are used in practicing these methods. Such methods are generally directed to environmental control fluids, the movement of such fluids through these systems, and the interaction of these fluids with other components of the system, e.g., other fluids or solid components of the system.Type: GrantFiled: April 23, 2012Date of Patent: March 17, 2015Assignee: Caliper Life Sciences, Inc.Inventors: J. Wallace Parce, Yung-mae M. Yao, Donald J. Morrissey, Jr.
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Publication number: 20150047980Abstract: The invention provides methods of performing a sizing analysis. In the methods, a sizing ladder used in performing the sizing analysis is corrected. In one method, the sizing ladder is corrected for batch-to-batch variations in a sieving gel. In another method, the sizing ladder is corrected for a sample concentration that is different from the archival sizing ladder concentration. Methods are also provided in which the sizing ladder is corrected using a standard marker in a sample and/or using a real-time standard sizing ladder. The methods may be used individually or in combination.Type: ApplicationFiled: October 28, 2014Publication date: February 19, 2015Applicant: Caliper Life Sciences, Inc.Inventors: Dan Camporese, Advit Bhatt, Josh Molho, Hui Xu, Ken Summers
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Patent number: 8934700Abstract: The invention provides a method and apparatus for isolating individual target cells. The apparatus includes a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction. A plurality of trapping channels intersect the collection channel, each trapping channel having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell. The apparatus also includes an imaging system configured to image individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyzes of the imaging data; and a directing system configured to direct the individual target cells. A pressure source is in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.Type: GrantFiled: March 4, 2013Date of Patent: January 13, 2015Assignee: Caliper Life Sciences, Inc.Inventors: Rajiv Bharadwaj, Bahram Fathollahi