Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.
Type:
Application
Filed:
June 27, 2016
Publication date:
December 29, 2016
Applicants:
CALIPER LIFE SCIENCES, INC., CANON U.S. LIFE SCIENCES, INC.
Inventors:
Steven A. Sundberg, Michael R. Knapp, Ivor T. Knight, Deborah J. Boles, Aaron Rulison, Wesley B. Dong, Andrew Fabans, Allen Boronkay, Edwawrd Donlon, Robert Moti, Michael Slater
Abstract: The invention provides a method and apparatus for isolating individual target cells. The apparatus includes a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction. A plurality of trapping channels intersect the collection channel, each trapping channel having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell. The apparatus also includes an imaging system configured to image individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyses of the imaging data; and a directing system configured to direct the individual target cells. A pressure source is in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.
Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.
Type:
Grant
Filed:
April 11, 2012
Date of Patent:
June 28, 2016
Assignees:
Caliper Life Sciences, Inc., Canon U.S. Life Sciences, Inc.
Inventors:
Steven A. Sundberg, Michael R. Knapp, Ivor T. Knight, Deborah J. Boles, Aaron J. Rulison, Wesley B. Dong, Edward P. Donlon, Robert J. Moti, Andrew G. Fabans, Allen R. Boronkay, Michael Slater
Abstract: An integrated microtomography and optical imaging system includes a rotating table that supports an imaging object, an optical stage, and separate optical and microtomography imaging systems. The table rotates the imaging object about a vertical axis running therethrough to a plurality of different rotational positions during a combined microtomography and optical imaging process. The optical stage can be a trans-illumination, epi-illumination or bioluminescent stage. The optical imaging system includes a camera positioned vertically above the imaging object. The microtomography system includes an x-ray source positioned horizontally with respect to the imaging object. Optical and x-ray images are both obtained while the imaging object remains in place on the rotating table. The stage and table are included within an imaging chamber, and all components are included within a portable cabinet.
Type:
Grant
Filed:
June 20, 2011
Date of Patent:
April 19, 2016
Assignee:
Caliper Life Sciences, Inc.
Inventors:
Daniel G. Stearns, David G. Nilson, Bradley W. Rice
Abstract: The invention provides devices, systems, and methods for extracting target objects from a sample. In the method, a stream of sample containing a plurality of target and non-target objects is directed by first and second streams of buffer through a sample inlet channel into a fluid junction and through the fluid junction into a sample waste channel. In response to detecting a target object within the stream of sample, an actuator is energized to close a normally open valve, resulting in a transient burst of cross-flow into the fluid junction that briefly diverts the flow of sample within the fluid junction and results in an aliquot of sample being directed into an aliquot delivery channel. The combination of the valve and the actuator acts as a self-limiting pulse generator.
Type:
Grant
Filed:
March 15, 2013
Date of Patent:
March 29, 2016
Assignee:
CALIPER LIFE SCIENCES, INC.
Inventors:
Joshua I. Molho, Daniel G. Stearns, I-Jane Chen, Danh Tran, Bradley W. Rice, Tobias Daniel Wheeler, Alexander V. Dukhovny
Abstract: The invention provides methods of controlling environmental conditions within a fluidic system, where such environmental conditions can affect the operation of the system in its desired function, and fluidic channels, devices, and systems that are used in practicing these methods. Such methods are generally directed to environmental control fluids, the movement of such fluids through these systems, and the interaction of these fluids with other components of the system, e.g., other fluids or solid components of the system.
Type:
Grant
Filed:
March 17, 2015
Date of Patent:
March 8, 2016
Assignee:
CALIPER LIFE SCIENCES, INC.
Inventors:
J. Wallace Parce, Yung-Mae M. Yao, Donald J Morrissey
Abstract: The invention provides a method and apparatus for isolating individual target cells. The apparatus includes a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction. A plurality of trapping channels intersect the collection channel, each trapping channel having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell. The apparatus also includes an imaging system configured to image individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyses of the imaging data; and a directing system configured to direct the individual target cells. A pressure source is in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.
Abstract: The present invention is an automated microfluidic chip processing apparatus that includes a deck for holding at least one microfluidic chip and capable of being accessed by a liquid handling system, a fluid control system, and a detection system, wherein a chip handling device transports the chip from the deck to the fluid control system and the detection system. The present invention also includes a chip for use with an automated microfluidic chip processing apparatus, and a method for processing a microfluidic chip using such an apparatus.
Type:
Grant
Filed:
July 29, 2013
Date of Patent:
December 8, 2015
Assignee:
Caliper Life Sciences, Inc.
Inventors:
Michael Greenstein, Colin B Kennedy, James C Mikkelsen, Jr.
Abstract: The invention provides a method and apparatus for isolating individual target cells. The apparatus includes a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction. A plurality of trapping channels intersect the collection channel, each trapping channel having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell. The apparatus also includes an imaging system configured to image individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyses of the imaging data; and a directing system configured to direct the individual target cells. A pressure source is in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.
Abstract: Method of using dopamine reuptake inhibitors, e.g., sydnonimine derivatives, for the management of diabetic symptoms and associated complications or conditions, such as hyperglycemia and diabetic neuropathy.
Type:
Grant
Filed:
December 3, 2010
Date of Patent:
September 1, 2015
Assignee:
Caliper Life Sciences, Inc.
Inventors:
Hao Chen, Ming Liu, Malathi Sathyamoorthy, Qi Su, Lisa Leary, Wayne Shaobin Zhong
Abstract: An array of transportable particle sets is used in a microfluidic device for performing chemical reactions in the microfluidic device. The microfluidic device comprises a main channel and intersecting side channels, the main channel and side channels forming a plurality of intersections. The array of particle sets is disposed in the main channel, and the side channels are coupled to reagents. As the particle sets are transported through the intersections of the main channel and the side channels, reagents are flowed through the side channels into contact with each array member (or selected array members), thereby providing a plurality of chemical reactions in the microfluidic system.
Type:
Grant
Filed:
January 27, 2011
Date of Patent:
August 11, 2015
Assignee:
Caliper Life Sciences, Inc.
Inventors:
Tammy Burd Mehta, Anne R. Kopf-Sill, J. Wallace Parce, Andrea W. Chow, Luc J. Bousse, Michael R. Knapp, Theo T. Nikiforov, Steve Gallagher
Abstract: The invention provides methods and apparatuses that allow a protein sample to undergo reduction, alkylation, and digestion in a continuous flow process carried out within a microfluidic device. Methods and apparatuses in accordance with the invention can be employed as part of an automated proteomics analysis carried out in an integrated proteomics system.
Type:
Grant
Filed:
April 3, 2006
Date of Patent:
August 4, 2015
Assignee:
Caliper Life Sciences, Inc.
Inventors:
Bahram Fathollahi, Javier A. Farinas, Andrea W. Chow, Stephane Mouradian
Abstract: A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip; electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region; applying a magnetic field to the magnetic holding region to fix the protein-attached beads in place within the magnetic holding region; binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.
Type:
Application
Filed:
January 15, 2015
Publication date:
July 16, 2015
Applicant:
CALIPER LIFE SCIENCES, INC.
Inventors:
Andrew Barry, Laurel Provencher, Seth Cohen, I-Jane Chen, Jun Yan, Jingjing Wang
Abstract: The invention provides a method and apparatus for isolating individual target cells. The apparatus includes a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction. A plurality of trapping channels intersect the collection channel, each trapping channel having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell. The apparatus also includes an imaging system configured to image individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyses of the imaging data; and a directing system configured to direct the individual target cells. A pressure source is in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.
Abstract: A microfluidic device with a filter includes a substrate; a flowpath including a well formed in the substrate in fluid communication with a channel formed in the substrate; and a filter disposed across the flowpath and associated with the channel.
Type:
Grant
Filed:
September 21, 2011
Date of Patent:
May 19, 2015
Assignee:
Caliper Life Sciences, Inc.
Inventors:
Stephane Mouradian, Josh Molho, Ming Wu, Hayley Wu, Javier A. Farinas