Patents Assigned to Capitalbio Corporation
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Patent number: 7918981Abstract: This invention relates generally to the field of cell separation or isolation. In particular, the invention provides a method for separating cells, which method comprises: a) selectively staining cells to be separated with a dye so that there is a sufficient difference in a separable property of differentially stained cells; and b) separating said differentially stained cells via said separable property. Preferably, the separable property is dielectrophoretic property of the differentially stained cells and the differentially stained cells are separated or isolated via dielectrophoresis. Methods for separating various types of cells in blood samples are also provided. Centrifuge tubes useful in density gradient centrifugation and dielectrophoresis isolation devices useful for separating or isolating various types of cells are further provided.Type: GrantFiled: November 14, 2006Date of Patent: April 5, 2011Assignees: CapitalBio Corporation, Tsinghua UniversityInventors: Gaoshan Jing, Jian Zhang, Jing Cheng
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Patent number: 7875466Abstract: This invention provides nanometer-sized fluorescent magnetic particles and processes of making them. The nanoparticle has a core particle comprising a magnetic material and a fluorescent material, and the particle size is less than about 1 micrometer. The nanoparticles can be coated with an inorganic or organic layer and can be surface-modified. The nanoparticles can be used in many biological assays.Type: GrantFiled: August 13, 2003Date of Patent: January 25, 2011Assignees: CapitalBio Corporation, Tsinghua UniversityInventors: Huachang Lu, Guangshun Yi, Depu Chen, Lianghong Guo, Jing Cheng
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Publication number: 20100260984Abstract: This invention relates generally to the field of moiety or molecule analysis, isolation, detection and manipulation and library synthesis. In particular, the invention provides a microdevice, which microdevice comprises: a) a substrate; and b) a photorecognizable coding pattern on said substrate. Preferably, the microdevice does not comprise an anodized metal surface layer. Methods and kits for isolating, detecting and manipulating moieties, and synthesizing libraries using the microdevices are also provided. The invention further provides two-dimensional optical encoders and uses thereof. In certain embodiments, the invention provides a microdevice, which microdevice comprises: a) a magnetizable substance; and b) a photorecognizable coding pattern, wherein said microdevice has a preferential axis of magnetization. Systems and methods for isolating, detecting and manipulating moieties and synthesizing libraries using the microdevices are also provided.Type: ApplicationFiled: April 14, 2010Publication date: October 14, 2010Applicants: AVIVA BIOSCIENCES CORPORATION, TSINGHUA UNIVERSITY, CAPITALBIO CORPORATIONInventors: Lei WU, Xiaobo Wang, Guoliang Tao, Junquan Xu, Jing Cheng, Mingxian Huang, Baoquan Sun, Wei Shao, Litian Liu, Depu Chen, David M. Rothwarf, Weiping Yang
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Patent number: 7776580Abstract: This invention relates generally to the field of cell seperation. In particular, the invention provides processes for isolating a target cell, cellular organelle or virus from a sample, using inter alia, nonspecific binding between a target cell, cellular organelle or virus with a magnetic microbead modified to comprise hydroxyl, carboxyl or epoxy groups.Type: GrantFiled: December 31, 2002Date of Patent: August 17, 2010Assignees: CapitalBio Corporation, Tsinghua UniversityInventors: Xu Zhang, Xin Xie, Depu Chen, Weiyang Fei, Jing Cheng
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Patent number: 7767438Abstract: This invention relates generally to the field of microarray reaction devices and uses thereof. In particular, the invention provides a microarray reaction device wherein a plurality of reaction spaces are formed between a first and second plurality of projections, the heights of said plurality of reaction spaces being substantially identical and controllable by a supporting structure, and the relative positions between the first and second plurality of projections being controllable by a positioning structure. Articles of manufacture comprising the microarray reaction device and methods for assaying an analyte using the microarray reaction device are also provided.Type: GrantFiled: March 28, 2003Date of Patent: August 3, 2010Assignee: CapitalBio CorporationInventors: Wanli Xing, Fei Jun Xian, Xianhua Wang, Jing Cheng
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Publication number: 20100184115Abstract: Methods and apparatus for designing and measuring a cell-electrode impedance sensor to detect chemical and biological samples, including biological cells. The method of designing a cell-electrode impedance sensor comprises: determining a cell free cell-electrode impedance and a cell-covered cell-electrode impedance; obtaining a sensor sensitivity of the cell-electrode impedance measurement system; and choosing one or more design parameters of the cell-electrode impedance sensor to maximize the sensor sensitivity. When the frequency of AC signal between electrodes ranges from 10 kHz to 40 kHz, the sensitivity of the sensor is maximized.Type: ApplicationFiled: July 25, 2007Publication date: July 22, 2010Applicant: CAPITALBIO CORPORATIONInventors: Wang Lei, Mitchelson Keith, Wang He, Cheng Jing
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Publication number: 20100151448Abstract: The present invention discloses an asymmetric PCR amplification method, its special primer and application, aims to provide a simple, effective PCR amplification for preparation of single-stranded product. The asymmetric PCR primer of the invention comprises some PCR primer pairs, in which an unrelated nucleic acids sequence to target sequence to be detected is added onto 5?-terminal of one primer. The asymmetric PCR amplification provided includes the steps: 1) preparative denaturing; 2) repetitiously denaturing, primers annealing, extending cycles as the first stage of PCR amplification; 3) repetitiously denaturing, primer extending cycles as the second stage of PCR amplification, wherein an unrelated nucleic acids sequence to target sequence to be detected is added onto 5?-terminal of one PCR primer of each pair in extension.Type: ApplicationFiled: November 22, 2004Publication date: June 17, 2010Applicant: CapitalBio CorporationInventors: Zhiwei Zhang, Can Wang, Lingxiang Zhu, Qiong Zhang, Jing Cheng
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Patent number: 7718362Abstract: This invention relates generally to the field of nucleic acid analysis. In particular, the invention provides a method for typing a target gene, using, inter alia, a chip comprising a support suitable for use in nucleic acid hybridization having immobilized thereon an oligonucleotide probe complementary to said target nucleotide sequence and at least one of the following oligonucleotide control probes: a positive control probe, a negative control probe, a hybridization control probe and an immobilization control probe. Oligonucleotide probes or probes arrays for typing a HLA target gene are also provided.Type: GrantFiled: July 18, 2003Date of Patent: May 18, 2010Assignees: CapitalBio Corporation, Tsinghua UniversityInventors: Huafang Gao, Xuemei Ma, Chi Zhang, Qian Chen, Yiming Zhou, Dong Wang, Yizhe Zhang, Yue Tian, Rui Zhang, Gengxin Lan, Yuxiang Zhou, Jing Cheng
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Patent number: 7706419Abstract: The invention provides a novel optical system for use in a microarray scanner, comprising an aperture-containing reflecting mirror comprising an aperture and a reflecting surface. The aperture of the aperture-containing reflecting mirror allows an excitation light to pass through, and the reflecting surface of the aperture-containing reflecting mirror allows emission light from a microarray to be reflected. The optical system may also comprises various other components such as laser generators, beam splitter, reflecting mirrors, excitation and emission light filters, excitation and emission objective lens, pinhole, and detector. The optical system described herein has high efficiency, high sensitivity, low background noise, structurally simple, and high versatility.Type: GrantFiled: June 3, 2005Date of Patent: April 27, 2010Assignee: Capitalbio CorporationInventors: Xianhua Wang, Guoliang Huang, Jing Cheng, Hong Zhang, Taishan Gao
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Patent number: 7615758Abstract: The invention provides a laser microarray scanner for microarray scanning, comprising an optical system, a scanning platform, and a data processing system. During scanning, the optical system remains fixed, and the microarray placed on the scanning platform moves relative to the optical system.Type: GrantFiled: June 2, 2005Date of Patent: November 10, 2009Assignee: Capitalbio CorporationInventors: Xianhua Wang, Hui Xu, Jianxin Ye, Yelei Sun, Guoliang Huang, Jing Cheng
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Publication number: 20090251155Abstract: Techniques, systems and apparatus are disclosed for detecting impedance. In one aspect, a microelectrode sensing device includes a substrate and an array of microelectrode sensors formed on the substrate. Each sensor includes at least one conductive layer formed above the substrate and patterned to include a counter electrode and multiple sensing electrodes to detect an electrical signal in absence and presence of one or more target cells positioned on at least a portion of a surface of each sensing electrode. The sensing electrodes are spaced apart and arranged around the counter electrode to provide a spatially averaged value of the detected electrical signal.Type: ApplicationFiled: October 24, 2008Publication date: October 8, 2009Applicants: CapitalBio Corporation, Tsinghua UniversityInventors: Lei Wang, Jing Zhu, Cheng Deng, Jing Cheng
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Patent number: 7578612Abstract: A low-cost, easy to operate, three-phase tilting agitator for microarrays, including large area microarrays, provides experimentally verified improvements in hybridization intensity and uniformity. Motion is coupled from a single motor to a sample holder via three suspension tethers. The microarrays may be immersed in a water bath during agitation to maintain a temperature for the hybridization reaction. The use of traditional cover slips for the microarrays minimizes the volume requirement for target sample solution.Type: GrantFiled: July 29, 2005Date of Patent: August 25, 2009Assignee: CapitalBio CorporationInventors: Liang Zhang, Fei J. Xian, Renyuan Chen, Lianshan Zhao, Jianxing Ye, Xianhua Wang
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Patent number: 7527719Abstract: The present invention provides a capillary electrophoresis chip apparatus for detecting nucleotide polymorphism or single nucleotide polymorphism belonging to capillary electrophoresis apparatuses. The apparatus comprises an upper channel layer comprising a one-, two-, or multi-dimensional microfluid channel and an electrode aperture structure for loading sample, a middle electrode layer for sealing the microfluid channel to form an intact capillary and providing the needed voltage for electrophoresis; and a lower heating layer for providing a stable temperature gradient for electrophoresis. The upper, middle and lower layers are thermal conductive and adhesive to each other.Type: GrantFiled: November 29, 2002Date of Patent: May 5, 2009Assignees: Capitalbio Corporation, Tsinghua UniversityInventors: Peng Liu, Wanli Xing, Dong Liang, Jing Cheng
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Publication number: 20080267828Abstract: The present invention relates to a micro-volume liquid ejection system, including an air pressure module, a micro-ejection unit which is connected with the air pressure module by means of pipes, and a control circuit which is connected with the air pressure module and the micro-ejection unit respectively. In the present invention, due to air is used as the pressure medium, on one hand, as the sample does not contact with the pressure regulating module, the efficiency of cleaning process is improved, on the other hand, the volume of the sample needed in the sample ejecting process is only equal to the cavity-dimension of the micro-ejection unit by not need to fill the whole pipe with liquid. During the sample ejecting process, it is not need to regulate the pressure. After the sample ejecting is finished, the sample can be put into its original place, so as to greatly save the sample.Type: ApplicationFiled: November 22, 2004Publication date: October 30, 2008Applicant: CAPITALBIO CORPORATIONInventors: Dong Wang, Kun Zou, Jianxing Ye, Yelei Sun, Hui Zhu, Xianhua Wang, Jing Cheng
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Publication number: 20080220979Abstract: This invention relates to the field of detecting nucleic acid molecules using microarrays. The invention provides a method for detecting a target nucleic acid molecule in a biological sample by hybridizing a cell lysate directly probes immobilized on microarrays without any nucleic acid purification.Type: ApplicationFiled: August 27, 2003Publication date: September 11, 2008Applicants: CAPITALBIO CORPORATION, TSINGHUA UNIVERSITYInventors: Dong Wang, Gang Li, Xuemei Ma, Chengxun Liu, Yuxiang Zhou, Jing Cheng
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Publication number: 20070054273Abstract: This invention relates generally to the field of microarray chips and uses thereof. In particular, the invention provides a microarray reaction device that can be used in assaying the interaction between various moieties, e.g., nucleic acids, immunoreactions involving proteins, interactions between a protein and a nucleic acid, a ligand-receptor interaction, and small molecule and protein or nucleic acid interactions, etc. Articles of manufacture and kits comprising the microarray reaction device and assaying methods using the microarray reaction device are also provided.Type: ApplicationFiled: August 13, 2003Publication date: March 8, 2007Applicant: CAPITALBIO CORPORATIONInventors: Feijun Xian, Wanli Xing, Jing Cheng, Dong Liang, Liang Zhang, Dong Wang
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Patent number: 7153648Abstract: This invention relates generally to the field of cell separation or isolation. In particular, the invention provides a method for separating cells, which method comprises: a) selectively staining cells to be separated with a dye so that there is a sufficient difference in a separable property of differentially stained cells; and b) separating said differentially stained cells via said separable property. Preferably, the separable property is dielectrophoretic property of the differentially stained cells and the differentially stained cells are separated or isolated via dielectrophoresis. Methods for separating various types of cells in blood samples are also provided. Centrifuge tubes useful in density gradient centrifugation and dielectrophoresis isolation devices useful for separating or isolating various types of cells are further provided.Type: GrantFiled: March 20, 2002Date of Patent: December 26, 2006Assignees: Capitalbio Corporation, Tsinghua UniversityInventors: Gaoshan Jing, Jian Zhang, Jing Cheng
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Patent number: 6998236Abstract: This invention relates generally to the field of microarray technology. In particular, the invention provides an integrated microarray device, which device comprises a substrate comprising a plurality of distinct microlocations and a plurality of microarray chips, wherein the number of said microlocations equals to or is more than the number of said microarray chips. In preferred embodiments, the devices also comprises a temperature controller at some or all of the microlocations. The use of the integrated microarray devices for detecting interactions among various moieties in various fields, such as clinical diagnostics, drug discovery, environmental monitoring and forensic analysis, etc., are further provided.Type: GrantFiled: August 5, 2002Date of Patent: February 14, 2006Assignees: Capitalbio Corporation, Tsinghua UniversityInventors: Wei Shao, Junquan Xu, Wan-Li Xing, Jing Cheng