Abstract: A microtome includes: a base element; a mid element movably connected to the base element via a joint; a first cutting apparatus element; a second cutting apparatus element; a first actuator apparatus configured to move the first cutting apparatus element relative to the mid element in a delivery direction in order thereby to set a delivery of the object to the blade; a measuring apparatus configured to measure a position of the mid element or of the first cutting apparatus element in the delivery direction relative to a reference; and a controller configured to control the first actuator apparatus on the basis of the position measured by the measuring apparatus. This can compensate the effect of the mechanical play of the joint on the first cutting apparatus element.

Abstract: A microscope objective for imaging a specimen using a microscope, the microscope objective being designed as an air objective for microscopy without an immersion medium or as an oil immersion objective for microscopy with an oil-based immersion medium or as a water immersion objective for microscopy with a water-based immersion medium. The front lens of the microscope objective is provided with a coating which repels an immersion medium and is lipophobic and hydrophobic if the objective is an air objective, only lipophobic if the objective is a water immersion objective, and only hydrophobic if the objective is an oil immersion objective.

Abstract: A detection optical unit (112) of an optical apparatus is configured to produce an imaged representation (151, 152) of a sample object (150) on a detector (114). An adjustable filter element (119) is arranged in a beam path of the detection optical unit (112) that defines the imaged representation (151, 152). A controller is configured to drive the adjustable filter element (119) to filter the spectrum of the beam path with a first filter pattern (301-308) and with a second filter pattern (301-308) and to drive the detector (114) to capture a first image, associated with the first filter pattern (301-308), and to capture a second image, which is associated with the second filter pattern (301-308). The controller is furthermore configured to determine a focus position (181) of the sample object (150) based on the first image and the second image. The first filter element (301-308) here defines a first line and the second filter element (301-308) defines a second line.

Abstract: A microscope system having a plurality of microscope modules connected to one another for data transfer purposes. The microscope system includes a central clock generator, the clock signal of which is provided to all microscope modules. The microscope modules are configured to use the clock signal or a clock derived therefrom as an internal clock. Moreover, a corresponding method for operating such a microscope system is described.

Abstract: A slide system for optical microscopy using immersion fluid including a cover glass having a first refractive index and a transparent film having a second refractive index. The second refractive index of the transparent film is matched to the first refractive index of the cover glass. The transparent film protects the cover glass from immersion fluid used for optical microscopy and the transparent film is configured such that the immersion fluid used can be removed by lifting off the transparent film.

Abstract: A microscopy method for three-dimensionally imaging an object, including imaging the object along a beam path into a first image on a first image plane. A first microlens array is arranged on the first image plane, and a second microlens array with the same pitch is arranged downstream of the first array. The two arrays laterally segment the first image and image same into a second image in which the segments are spaced apart and separated by gaps. On a pupil plane downstream of the microlens array, a provided phase mask generates a spot for each segment of the second image according to a pixel diffusion function. A detector detects the shape and structure of the spot, and a controller ascertains a lateral intensity distribution and depth specification from the shape and/or structure of the spot for each segment and generates a depth-resolved image of the object therefrom.

Abstract: A light microscope comprises: a structuring optical unit comprising a waveguide chip for providing a structured illumination; an input selection device for variably directing light to one of several inputs of the waveguide chip; the waveguide chip further comprising a light guide path following each of the inputs; each light guide path divides into several path divisions; and each path division leads to one output of the wave-guide chip. The outputs of the waveguide chip can be arranged at a pupil plane of the light microscope, and an exit direction of light from the outputs is transverse to a plane defined by the waveguide chip. A method for providing structured illumination light using the light microscope is also described.

Abstract: An illumination unit for microscopes has an MID structure for the free spatial arrangement of different electronic and optical components, so that surfaces of a three-dimensional carrier are available as a replacement for printed circuit boards. The MID structure designed as an illumination unit contains a closed annular main part having an annular free beam opening, where columns are arranged on the main part. On the free ends of the columns, illumination means are provided which can be contacted via conductor paths provided on the surface of the MID structure. The columns contain a cooling structure on a side surface facing away from the free beam opening, and a plug for the power supply and a control unit for controlling the illumination means are provided on the main part of the MID structure, The MID structure designed as an illumination unit is provided in a housing of a microscope objective.

Abstract: Various approaches in which an image-recording parameter is varied between a plurality of images of an object and a stereo image pair is displayed on the basis of the images recorded thus are described. Here, in particular, the image-recording parameter can be a focal plane or an illumination direction.

Abstract: Various examples relate to determining a number and/or a confluency of cells in a microscopy image. To that end, the microscopy image is firstly rescaled and then processed.

Abstract: The invention relates to a localization microscopy method for localizing signal sources. Here, at least once for each pixel of a detector, values of an error parameter are ascertained and stored in a calibration data record in a manner assigned to the relevant pixel. Captured image data are used to identify regions of origin of signal sources and fit a point spread function to the pixel values of the respective regions of origin. The respective signal source is localized on the basis of the point spread function. The pixel-specific error parameter of each pixel can be compared to a threshold. If the threshold is exceeded, these pixels are either ignored or replaced by means of interpolation when fitting the point spread function. In addition or as an alternative thereto, the real noise performance of the pixels is ascertained and corrected on the basis of derived pixel-specific error parameters.

Abstract: One or more images of a sample may be split into image data split according to dyes. The sample has at least two different dyes, in particular fluorescent dyes. The method for splitting the one or more images includes providing the one or more images of the sample and inputting the one or more images into a machine learning system. The method then includes generating the image data split according to dyes from the image or the images, using the machine learning system. The machine learning system removes at least one partial structure of the sample that is present in the image data split according to dyes of more than one dye from the image data of one or more dyes.

Abstract: An optical assembly for scanning excitation radiation and/or manipulation radiation in a laser scanning microscope. The assembly an optical scanning unit as a first focusing device for providing a first pupil plane, a first beam deflecting device, which is made of a first scanner arranged in the first pupil plane, for scanning the excitation radiation and/or manipulation radiation in a first coordinate direction, and a second focusing device for generating a second pupil plane, which is optically conjugated to the first pupil plane. A second beam deflecting device is provided for deflecting the excitation radiation and/or manipulation radiation, said second deflecting device being arranged in the second pupil plane. A third focusing device is provided in order to generate a third pupil plane, optically conjugated to the first pupil plane.

Abstract: The system described herein relates to a method for operating a beam apparatus, such as a particle beam apparatus or laser beam apparatus, a computer program product and a beam apparatus for carrying out the method, and to an object holder for an object that, for example, is able to be arranged in a particle beam apparatus. The method includes generating a marking on an object holder using a laser beam of a laser beam device and/or using a particle beam of the particle beam apparatus, where the particle beam includes charged particles, arranging an object on the object holder, moving the object holder, positioning the particle beam and/or the laser beam in relative fashion in relation to the object using the marking, and processing, imaging and/or analyzing the object using the particle beam and/or the laser beam.

Abstract: The invention relates firstly to a method for determining a mechanical deviation on a displacement path of an optical zoom lens (03), in particular on a displacement path of an optical zoom lens (03) of a microscope. The optical zoom lens (03) is arranged in a beam path (01) between an object (19) to be recorded and an electronic image sensor (04). In a first method step, an optical marker is introduced into the beam path (01) at a position of the beam path (01) located between the object (19) to be recorded and the optical zoom lens (03), such that the optical marker passes the optical zoom lens (03) and then is depicted on an image in which a position of the optical marker is detected and determined. This is compared with a reference position of the optical marker in order to determine the mechanical deviation on the displacement path of the optical zoom lens (03).

Abstract: In a method for deconvolving image data, image data of an object are captured with a number n of confocal beam paths. The image data are converted into resultant image data by means of a point spread function. The resultant image data are deconvolved again in the frequency domain using a deconvolution function, wherein the deconvolution function contains the formation of at least a number n of sum terms and a Wiener parameter w. The results of the sum terms are stored in retrievable fashion; the Wiener parameter W is modified at least once proceeding from its original value and the deconvolution is carried out by means of the deconvolution function with the modified Wiener parameter w and the stored results of the sum terms.

Abstract: The invention relates first to a camera module for a microscope. The camera module comprises an optical interface for inserting the camera module into an imaging beam path of the microscope, which imaging beam path images a nominal intermediate image. Furthermore, the camera module comprises an electronic image converter and a functional element for changing an intermediate image plane, which functional element is arranged in a beam path of the camera module between the optical interface and the image converter. The camera module also comprises a first optical assembly having an optical power, which first optical assembly is arranged in the beam path between the optical interface and the image converter. According to the invention, a distance between the functional element and the optical interface along the beam path or a distance between the first optical assembly and the optical interface along the beam path can be changed. The invention further relates to a method for operating the camera module.

Abstract: A microscope system includes a microscope with at least one microscope sensor. Each microscope sensor has a measurement device for recording sample signals; an analog-to-digital converter for converting recorded sample signals to digital data; a data compression device which produces a compressed data stream from the digital data; and a data output interface, which outputs the compressed data stream and a raw data stream that comprises digital data that were not compressed. The microscope system additionally includes a user computer to which the compressed data stream is transmitted and also a data memory to which the raw data stream is transmitted. The user computer calculates real-time images from the compressed data stream and reads and processes the raw data stream from the data memory for subsequent data analysis. In addition, a method for operating such a microscope system is described.

Abstract: A particle beam generator for a particle beam device may be operated. A liquid metal may be provided from a container of a particle source to an emission device of the particle source and a first heating cycle for cleaning the particle source performed, which may comprise supplying a heating current to a heating device arranged at the emission device using a current supply unit, heating the emission device during a heating time period, measuring a value of a voltage drop at the heating device and/or at the current supply unit and adjusting at least one of: the heating current and the heating time period using the current supply unit, depending on the measured value of the voltage drop. A second heating cycle for cleaning the particle source may include using at least one of: the adjusted heating current and the adjusted heating time period, for heating the emission device.

Abstract: A method for executing and translating a computer program in a computer network, in particular for controlling a microscope, wherein in the case of at least one function call to the relevant second function, multiple return values from multiple preceding function calls are transferred as separate parameters. This allows significantly more complex and flexible function calls. More flexibility in the execution of container-based functions of a computer program in a computer network can additionally be achieved in that the computer program has not only function calls but also instructions for controlling the program flow, for example instructions for the conditional execution of program sections and jump instructions. The development of container-based computer programs can be simplified and speeded up by providing a database that contains the execution requirements of the function modules and also the interface definitions thereof. This permits a static typing of variables.