Patents Assigned to Cellular Dynamics International, Inc.
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Patent number: 8765470Abstract: Methods and composition for providing induced pluripotent stem (iPS) cells are provided. For example, in certain aspects methods including reprogramming B lymphocytes transformed by episomal vectors such as Epstein-Barr virus-based vectors are described. Furthermore, the invention provides induced pluripotent stem cells essentially free of exogenous elements and having B cell immunoglobin variable region rearrangement.Type: GrantFiled: August 3, 2011Date of Patent: July 1, 2014Assignee: Cellular Dynamics International, Inc.Inventors: James Thomson, Deepika Rajesh, Sarah Jane Dickerson, Amanda Mack, Michael Miller
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Patent number: 8741648Abstract: Methods and compositions relating to the production of induced pluripotent stem cells (iPS cells) are disclosed. For example, induced pluripotent stem cells may be generated from CD34+ hematopoietic cells, such as human CD34+ blood progenitor cells, or T cells. Various iPS cell lines are also provided. In certain embodiments, the invention provides novel induced pluripotent stem cells with a genome comprising genetic rearrangement of T cell receptors.Type: GrantFiled: June 4, 2010Date of Patent: June 3, 2014Assignee: Cellular Dynamics International, Inc.Inventors: Deepika Rajesh, Amanda Mack
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Patent number: 8691574Abstract: Methods and compositions relating to the production of induced pluripotent stem cells (iPS cells) are disclosed. For example, induced pluripotent stem cells may be generated from peripheral blood cells, such as human blood progenitor cells, using episomal reprogramming and feeder-free or xeno-free conditions. In certain embodiments, the invention provides novel methods for improving overall reprogramming efficiency with low number of blood progenitor cells.Type: GrantFiled: June 14, 2011Date of Patent: April 8, 2014Assignee: Cellular Dynamics International, Inc.Inventor: Amanda Mack
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Publication number: 20140037600Abstract: The invention generally regards methods for providing ematopoietic cells and precursors of hematopoietic cells from a variety of cell sources, such as pluripotent stem cells or somatic cells. Also provided are therapeutic compositions including the provided hematopoietic cells and precursors of hematopoietic cells, and methods of using such for the treatment of subjects.Type: ApplicationFiled: February 7, 2012Publication date: February 6, 2014Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.Inventors: Junying Yu, Maksym A. Vodyanyk
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Publication number: 20140038293Abstract: Methods and composition of induction of pluripotent stem cells and other desired cell types are disclosed. For example, in certain aspects methods for generating essentially vector-free induced pluripotent stem cells are described. Furthermore, the invention provides induced pluripotent stem cells and desired cell types essentially free of exogenous vector elements with the episomal expression vectors to express differentiation programming factors.Type: ApplicationFiled: August 7, 2013Publication date: February 6, 2014Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.Inventor: Amanda MACK
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Publication number: 20130316445Abstract: The invention concerns methods for automated culture of embryonic stem cells (ESCs) such as human ESCs. In some aspects, methods of the invention employ optimized culture media and limited proteolytic treatment of cells to separate cell clusters for expansion. Automated systems for passage and expansion of ESCs are also provided.Type: ApplicationFiled: March 19, 2013Publication date: November 28, 2013Applicant: Cellular Dynamics International, Inc.Inventors: Nathaniel BEARDSLEY, Veit BERGENDAHL, Megan FITZGERALD, Christine DAIGH
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Patent number: 8557580Abstract: The present invention provides methods and compositions for the production of hematopoietic progenitor cells or endothelial progenitor cells from human pluripotent stem cells using a defined cell culture medium without the need to utilize feeder cells or serum. In some embodiments, differentiation is accomplished using hypoxic atmospheric conditions. The defined medium of the present invention may contain growth factors and a matrix component. The hematopoietic progenitor cells may be further differentiated into cell lineages including red blood cells, macrophages, granulocytes, and megakaryocytes. The endothelial progenitor cells may be further differentiated into endothelial cells. Also disclosed are screening assays for identification of candidate substances that affect differentiation of pluripotent stem cells into progenitor cells.Type: GrantFiled: February 22, 2010Date of Patent: October 15, 2013Assignee: Cellular Dynamics International, Inc.Inventors: Christine Daigh, Peter Fuhrken, Giorgia Salvagiotto
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Patent number: 8546140Abstract: Methods and composition of induction of pluripotent stem cells and other desired cell types are disclosed. For example, in certain aspects methods for generating essentially vector-free induced pluripotent stem cells are described. Furthermore, the invention provides induced pluripotent stem cells and desired cell types essentially free of exogenous vector elements with the episomal expression vectors to express differentiation programming factors.Type: GrantFiled: June 4, 2009Date of Patent: October 1, 2013Assignee: Cellular Dynamics International, Inc.Inventors: Amanda Mack, James Thomson
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Publication number: 20130251694Abstract: The invention generally features methods for providing hepatocytes from a variety of cell sources, particularly pluripotent stem cells, therapeutic compositions featuring such cells, and methods of using them for the treatment of subjects.Type: ApplicationFiled: June 5, 2013Publication date: September 26, 2013Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.Inventors: Junying YU, Fongching Kevin CHAU, Jinlan JIANG, Yong JIANG, Maksym A. VODYANYK
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Patent number: 8513012Abstract: Provided are methods for generating mast cells from pluripotent stem cells in vitro. Methods are disclosed for the differentiation of pluripotent cells, such as iPS cells and/or human embryonic stem cells, into mast cells. The resulting mast cells may be used for various purposes including screening cells for drug development and research. Growth factors which may be included in culture media according to the present invention include stem cell factor (SCF), FLT-3 ligand, thrombopoietin (TPO), interleukin-3 (IL-3), and/or interleukin-6 (IL-6).Type: GrantFiled: May 4, 2009Date of Patent: August 20, 2013Assignee: Cellular Dynamics International, Inc.Inventors: Christine Daigh, Deepika Rajesh
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Publication number: 20130210141Abstract: Provided herein are methods for the in vitro maintenance, expansion, culture, and/or differentiation of pluripotent cells, such as human embryonic stem cells (hESC) or induced pluripotent cells (iPSC), into hematopoietic precursor cells or endothelial cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the pluripotent cells into hematopoietic precursor cells or endothelial cells. The resulting hematopoietic precursor cells may be further differentiated into various myeloid or lymphoid lineages.Type: ApplicationFiled: February 1, 2013Publication date: August 15, 2013Applicant: Cellular Dynamics International, Inc.Inventor: Cellular Dynamics International, Inc.
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Publication number: 20130196437Abstract: Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.Type: ApplicationFiled: March 11, 2013Publication date: August 1, 2013Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.Inventor: Cellular Dynamics International, Inc.
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Publication number: 20130196436Abstract: Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.Type: ApplicationFiled: March 11, 2013Publication date: August 1, 2013Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.Inventor: Cellular Dynamics International, Inc.
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Publication number: 20130189778Abstract: Methods and composition of induction of pluripotent stem cells and other desired cell types are disclosed. For example, in certain aspects methods for generating essentially vector-free induced pluripotent stem cells are described. Furthermore, the invention provides induced pluripotent stem cells and desired cell types essentially free of exogenous vector elements with the episomal expression vectors to express differentiation programming factors.Type: ApplicationFiled: March 11, 2013Publication date: July 25, 2013Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.Inventor: CELLULAR DYNAMICS INTERNATIONAL, INC.
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Patent number: 8481317Abstract: The invention generally features methods for providing hepatocytes from a variety of cell sources, particularly pluripotent stem cells, therapeutic compositions featuring such cells, and methods of using them for the treatment of subjects.Type: GrantFiled: April 13, 2011Date of Patent: July 9, 2013Assignee: Cellular Dynamics International, Inc.Inventors: Junying Yu, Fongching Kevin Chau, Jinlan Jiang, Yong Jiang, Maksym A. Vodyanyk
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Patent number: 8415155Abstract: Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.Type: GrantFiled: October 19, 2010Date of Patent: April 9, 2013Assignee: Cellular Dynamics International, Inc.Inventors: Casey Stankewicz, Matt Riley, Nathaniel Beardsley, Wen Bo Wang, Peter Fuhrken, Steven Kattman
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Patent number: 8372642Abstract: Provided herein are methods for the in vitro maintenance, expansion, culture, and/or differentiation of pluripotent cells, such as human embryonic stem cells (hESC) or induced pluripotent cells (iPSC), into hematopoietic precursor cells or endothelial cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the pluripotent cells into hematopoietic precursor cells or endothelial cells. The resulting hematopoietic precursor cells may be further differentiated into various myeloid or lymphoid lineages.Type: GrantFiled: March 1, 2010Date of Patent: February 12, 2013Assignee: Cellular Dynamics International, Inc.Inventors: Deepika Rajesh, Rachel Lewis
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Publication number: 20120322670Abstract: Methods for the determination of tissue-specific genetic variation are provided. For example, in certain aspects methods for using iPS cell-derived specific cell types for differential molecular analysis of tissue-specific genetic variation are described.Type: ApplicationFiled: August 26, 2010Publication date: December 20, 2012Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.Inventors: James Thomson, Nicholas Seay
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Publication number: 20120171771Abstract: Methods and composition for generation of genetically modified induced pluripotent stem cells and hematopoietic cell derived therefrom are provided. For example, in certain aspects those cells comprise a modified gene structure related to HIV cellular entry, such as CCR5 mutants.Type: ApplicationFiled: July 7, 2010Publication date: July 5, 2012Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.Inventors: James Thomson, Nicholas Seay
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Publication number: 20120009618Abstract: The invention generally regards methods for providing endothelial cells and precursors of endothelial cells from a variety of cell sources, such as pluripotent stem cells. Also provided are therapeutic compositions including the provided endothelial cells, and methods of using them for the treatment of subjects.Type: ApplicationFiled: July 7, 2011Publication date: January 12, 2012Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.Inventors: Junying Yu, Maksym A. Vodyanyk