Patents Assigned to Cellular Dynamics International, Inc.
  • METHOD FOR REPRODUCIBLE DIFFERENTIATION OF CLINICAL-GRADE RETINAL PIGMENT EPITHELIUM CELLS
    Publication number: 20190169569
    Abstract: Provided herein are methods of producing an RPE cell population from a starting cell suspension, such as a single cell suspension, of pluripotent stem cells (PSCs). Such a method may comprise culturing the starting single cell suspension of PSCs in differentiation media to produce human RPE cells.
    Type: Application
    Filed: September 7, 2016
    Publication date: June 6, 2019
    Applicants: The United States of America, as represented by the Secretary, Department of Health and Human Serv, Cellular Dynamics International, Inc.
    Inventors: Kapil Bharti, Lucas Chase, Feng Xuezhu, Balendu Shekhar Jha
  • METHODS FOR THE PRODUCTION OF IPS CELLS USING NON-VIRAL APPROACH
    Publication number: 20180340150
    Abstract: Methods and composition of induction of pluripotent stem cells and other desired cell types are disclosed. For example, in certain aspects methods for generating essentially vector-free induced pluripotent stem cells are described. Furthermore, the invention provides induced pluripotent stem cells and desired cell types essentially free of exogenous vector elements with the episomal expression vectors to express differentiation programming factors.
    Type: Application
    Filed: December 15, 2017
    Publication date: November 29, 2018
    Applicant: Cellular Dynamics International, Inc.
    Inventor: Amanda MACK
  • Cardiomyocyte production
    Patent number: 9957482
    Abstract: Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.
    Type: Grant
    Filed: March 23, 2015
    Date of Patent: May 1, 2018
    Assignee: Cellular Dynamics International, Inc.
    Inventors: Casey Stankewicz, Matt Riley, Nathaniel Beardsley, Wen Bo Wang, Peter Fuhrken, Steven Kattman
  • METHODS FOR DIFFERENTIATING PLURIPOTENT CELLS
    Publication number: 20180051248
    Abstract: Methods are provided, in some aspects, for differentiating pluripotent cells into midbrain dopaminergic (DA) neurons using a mono-SMAD inhibition or inhibition of SMAD signaling with only one SMAD inhibitor. In some embodiments, mono-SMAD inhibition utilizes a single inhibitor of bone morphogenic protein (BMP) for differentiating pluripotent cells into midbrain DA neurons.
    Type: Application
    Filed: August 16, 2017
    Publication date: February 22, 2018
    Applicant: Cellular Dynamics International, Inc.
    Inventors: Christopher W. McMAHON, Lauren E. LITTLE, Wen Bo WANG, Nathaniel A. Elliott
  • PRODUCTION OF MIDBRAIN DOPAMINERGIC NEURONS AND METHODS FOR THE USE THEREOF
    Publication number: 20180021383
    Abstract: Methods are provided for efficient production of midbrain dopaminergic (DA) neurons. In some aspects, methods involve differentiation and selection of DA neurons for a transgenic pluripotent cell population (e.g., cells comprising a selectable marker gene). Cell populations produced by the instant methods and methods of their use are likewise provided.
    Type: Application
    Filed: June 6, 2017
    Publication date: January 25, 2018
    Applicant: Cellular Dynamics International, Inc.
    Inventors: Matt GEORGE, Carrie CHAVEZ, Chris McMAHON, Wen Bo WANG, Lucas CHASE, Brad SWANSON
  • REPROGRAMMING T CELLS AND HEMATOPOIETIC CELLS
    Publication number: 20170226484
    Abstract: Methods and compositions relating to the production of induced pluripotent stem cells (iPS cells) are disclosed. For example, induced pluripotent stem cells may be generated from CD34+ hematopoietic cells, such as human CD34+ blood progenitor cells, or T cells. Various iPS cell lines are also provided. In certain embodiments, the invention provides novel induced pluripotent stem cells with a genome comprising genetic rearrangement of T cell receptors.
    Type: Application
    Filed: April 26, 2017
    Publication date: August 10, 2017
    Applicant: Cellular Dynamics International, Inc.
    Inventors: Matthew BROWN, Elizabeth Rondon DOMINGUEZ, Randy LEARISH, Emile NUWAYSIR, Deepika RAJESH, Amanda MACK
  • CARDIOMYOCYTE PRODUCTION
    Publication number: 20170226481
    Abstract: Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.
    Type: Application
    Filed: April 27, 2017
    Publication date: August 10, 2017
    Applicant: Cellular Dynamics International, Inc.
    Inventors: Casey STANKEWICZ, Matt RILEY, Nathaniel BEARDSLEY, Wen Bo WANG, Peter FUHRKEN, Steven KATTMAN
  • Production of midbrain dopaminergic neurons and methods for the use thereof
    Patent number: 9694036
    Abstract: Methods are provided for efficient production of midbrain dopaminergic (DA) neurons. In some aspects, methods involve differentiation and selection of DA neurons for a transgenic pluripotent cell population (e.g., cells comprising a selectable marker gene). Cell populations produced by the instant methods and methods of their use are likewise provided.
    Type: Grant
    Filed: March 20, 2015
    Date of Patent: July 4, 2017
    Assignee: Cellular Dynamics International, Inc.
    Inventors: Matt George, Carrie Chavez, Chris McMahon, Wen Bo Wang, Lucas Chase, Brad Swanson
  • Methods for the production of IPS cells using Epstein-Barr (EBV)-based reprogramming vectors
    Patent number: 9644184
    Abstract: Methods and composition of induction of pluripotent stem cells and other desired cell types are disclosed. For example, in certain aspects methods for generating essentially vector-free induced pluripotent stem cells are described. Furthermore, the invention provides induced pluripotent stem cells and desired cell types essentially free of exogenous vector elements with the episomal expression vectors to express differentiation programming factors.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: May 9, 2017
    Assignee: Cellular Dynamics International, Inc.
    Inventor: Amanda Mack
  • GENERATION OF INDUCED PLURIPOTENT STEM CELLS FROM SMALL VOLUMES OF PERIPHERAL BLOOD
    Publication number: 20170088818
    Abstract: Methods and compositions relating to the production of induced pluripotent stem cells (iPS cells) are disclosed. For example, induced pluripotent stem cells may be generated from peripheral blood cells, such as human blood progenitor cells, using episomal reprogramming and feeder-free or xeno-free conditions. In certain embodiments, the invention provides novel methods for improving overall reprogramming efficiency with low number of blood progenitor cells.
    Type: Application
    Filed: August 10, 2016
    Publication date: March 30, 2017
    Applicant: Cellular Dynamics International, Inc.
    Inventor: Amanda MACK
  • MACS-BASED PURIFICATION OF STEM CELL-DERIVED RETINAL PIGMENT EPITHELIUM
    Publication number: 20170067017
    Abstract: Provided herein are methods of enriching a retinal pigment epithelium (RPE) cell population derived from stem cells. Such a method may comprise removing contaminating cells through the depletion of CD24 positive cells, CD56 positive cells, and/or CD90 positive cells from a starting population of RPE cells.
    Type: Application
    Filed: September 7, 2016
    Publication date: March 9, 2017
    Applicant: Cellular Dynamics International, Inc.
    Inventors: Nathan MEYER, Lucas CHASE, Casey STANKEWICZ
  • Hematopoietic precursor cell production by programming
    Patent number: 9574179
    Abstract: The invention generally regards methods for providing hematopoietic cells and precursors of hematopoietic cells from a variety of cell sources, such as pluripotent stem cells or somatic cells. Also provided are therapeutic compositions including the provided hematopoietic cells and precursors of hematopoietic cells, and methods of using such for the treatment of subjects.
    Type: Grant
    Filed: February 7, 2012
    Date of Patent: February 21, 2017
    Assignee: Cellular Dynamics International, Inc.
    Inventors: Junying Yu, Maksym A. Vodyanyk
  • Identifying genetic variation in affected tissues
    Patent number: 9506114
    Abstract: Methods for the determination of tissue-specific genetic variation are provided. For example, in certain aspects methods for using iPS cell-derived specific cell types for differential molecular analysis of tissue-specific genetic variation are described.
    Type: Grant
    Filed: August 26, 2010
    Date of Patent: November 29, 2016
    Assignee: Cellular Dynamics International, Inc.
    Inventors: James Thomson, Nicholas Seay
  • Priming of pluripotent stem cells for neural differentiation
    Patent number: 9487752
    Abstract: Methods and composition for differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including priming stem cells for neural differentiation in a culture medium essentially free of growth factors such as FGF and TGF?. As an advantage, the neural cells may be provided with improved consistency and purity.
    Type: Grant
    Filed: March 30, 2012
    Date of Patent: November 8, 2016
    Assignee: Cellular Dynamics International, Inc.
    Inventors: Nathan Meyer, Matthew George, Casey Stankewicz, Deepika Rajesh
  • METHODS FOR CELL REPROGRAMMING AND GENOME ENGINEERING
    Publication number: 20160281063
    Abstract: Methods for producing engineered induced pluripotent stem (iPS) cells are provided comprising introducing a first nucleic acid into somatic cells for integration into their genome and reprogramming the cells to produce engineered iPS cells having the nucleic acid integrated into their genome. For example, in certain aspects the cells are reprogrammed by introduction of a genetic element that expresses one or more reprogramming factor and culturing of the cells under conditions sufficient to produce reprogrammed cells.
    Type: Application
    Filed: February 23, 2016
    Publication date: September 29, 2016
    Applicant: Cellular Dynamics International, Inc.
    Inventors: Thomas J. BURKE, Michael MILLER, Michael McLACHLAN, Sarah Jane DICKERSON, Anne STROUSE
  • Generation of induced pluripotent stem cells from small volumes of peripheral blood
    Patent number: 9447382
    Abstract: Methods and compositions relating to the production of induced pluripotent stem cells (iPS cells) are disclosed. For example, induced pluripotent stem cells may be generated from peripheral blood cells, such as human blood progenitor cells, using episomal reprogramming and feeder-free or xeno-free conditions. In certain embodiments, the invention provides novel methods for improving overall reprogramming efficiency with low number of blood progenitor cells.
    Type: Grant
    Filed: February 12, 2014
    Date of Patent: September 20, 2016
    Assignee: Cellular Dynamics International, Inc.
    Inventor: Amanda Mack
  • REPROGRAMMING T CELLS AND HEMATOPOIETIC CELLS
    Publication number: 20160257939
    Abstract: Methods and compositions relating to the production of induced pluripotent stem cells (iPS cells) are disclosed. For example, induced pluripotent stem cells may be generated from CD34+ hematopoietic cells, such as human CD34+blood progenitor cells, or T cells. Various iPS cell lines are also provided. In certain embodiments, the invention provides novel induced pluripotent stem cells with a genome comprising genetic rearrangement of T cell receptors.
    Type: Application
    Filed: April 27, 2016
    Publication date: September 8, 2016
    Applicant: Cellular Dynamics International, Inc.
    Inventors: Matthew BROWN, Elizabeth Rondon DOMINGUEZ, Randy LEARISH, Emile NUWAYSIR, Deepika RAJESH, Amanda MACK
  • Endothelial cell production by programming
    Patent number: 9404122
    Abstract: The invention generally regards methods for providing endothelial cells and precursors of endothelial cells from a variety of cell sources, such as pluripotent stem cells. Also provided are therapeutic compositions including the provided endothelial cells, and methods of using them for the treatment of subjects.
    Type: Grant
    Filed: June 12, 2014
    Date of Patent: August 2, 2016
    Assignee: Cellular Dynamics International, Inc.
    Inventors: Junying Yu, Maksym A. Vodyanyk
  • Cardiomyocyte production
    Patent number: 9365827
    Abstract: Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: June 14, 2016
    Assignee: Cellular Dynamics International, Inc.
    Inventors: Casey Stankewicz, Matt Riley, Nathaniel Beardsley, Wen Bo Wang, Peter Fuhrken, Steven Kattman
  • Cardiomyocyte medium with dialyzed serum
    Patent number: 9365826
    Abstract: Methods and composition for maintenance of cardiomyocytes are provided. For example, in certain aspects methods including culturing the cardiomyocytes in a medium essentially free of serum or containing dialyzed serum to maintain long-term purity. In further aspects, methods for modulation of cardiomyocytes may be provided.
    Type: Grant
    Filed: June 20, 2011
    Date of Patent: June 14, 2016
    Assignee: Cellular Dynamics International, Inc.
    Inventors: Nathan Meyer, Brad Swanson, Steve Fiene