Abstract: The present invention relates to a trypsin-free cell stamp system and a use thereof. According to the present invention, an increase in the passage number of stem cells can be prevented compared with conventional methods of isolating cells from a cell culture dish, while providing a support, which is an essential condition of cell growth, by introducing the trypsin-free cell stamp system, and cells can be continuously supplied for a polymer-based fiber support without an additional subculture process since the empty space of a cell culture dish is filled as times passes. In addition, the artificial effects on cells can be minimized since the cells migrate to a polymer-based nano/micro-fiber support without other external stimulation, and thus the potency of stem cells is increased, thereby inducing more effective differentiation, such that the present invention, as a cell therapeutic agent, can be utilized in general fields of regenerative medicine and tissue engineering.
Type:
Application
Filed:
October 20, 2015
Publication date:
October 26, 2017
Applicant:
COLLEGE OF MEDICINE POCHON CHA UNIVERSITY INDUSTRY -ACADEMIC COOPERATION FOUNDATION
Inventors:
Han Soo PARK, Ah Yeon RYANG, Ha Ram LEE, Soo Hong LEE, In Bo HAN, Byoung Ju KIM
Abstract: The present invention provides a method for proliferating neural progenitor cells and a composition for treating neurological diseases, the composition including a proliferated neural progenitor cell. When a fetal neural progenitor cell is cultured under a hypoxia condition and/or in a medium containing tocoperol, tocoperol acetate, or a mixture thereof, the improved cell proliferation rates of the fetal neural progenitor cell are confirmed. In addition, considering an effect of the neural progenitor cell on preventing differentiation thereof into neurons at the time of proliferation, the present disclosure may contribute to mass production of neural stem cells, and accordingly, the proliferated neural progenitor cell is expected to be utilized in the treatment of a neurological disease.
Type:
Application
Filed:
October 10, 2014
Publication date:
October 19, 2017
Applicant:
COLLEGE OF MEDICINE POCHON CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
Abstract: The present invention provides a method for differentiating human neural progenitor cells into dopaminergic neurons, comprising the step of culturing human neural progenitor cells in a medium containing fusaric acid. In addition, the present invention provides a medium for differentiation of human neural progenitor cells into dopaminergic neurons.
Type:
Application
Filed:
June 7, 2011
Publication date:
April 11, 2013
Applicant:
College of Medicine Pochon Cha University Industry - Academic Cooperation Foundation
Abstract: The present invention provides a medium for maintaining stemness of stem cells comprising a protein kinase C inhibitor in a basal medium; and a method for culturing stem cells while maintaining stemness thereof, using the same. And also, the present invention provides a medium for inducing differentiation of stem cells comprising a protein kinase C activator in a basal medium; and a method for inducing differentiation of stem cells, using the same.
Type:
Application
Filed:
March 18, 2011
Publication date:
September 20, 2012
Applicant:
COLLEGE OF MEDICINE POCHON CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
Abstract: Provided are methods for the vitrification of human oocytes, which comprises: (a) placing human oocytes on a transfer instrument; and (b) placing the transfer instrument and the human oocytes directly into a slushed nitrogen (N2 slush), wherein the human oocytes are directly exposed to the N2 slush thereby undergoing vitrification, and wherein the human oocytes are able to live for a period of time after the human oocytes are devitrified.
Type:
Grant
Filed:
April 17, 2006
Date of Patent:
May 24, 2011
Assignees:
Chabio&Diostech Co. Ltd., College of Medicine Pochon Cha University Industry-Academic Cooperation Foundation
Inventors:
Tae Ki Yoon, Dong Ryul Lee, Hyung Min Chung, Kwang Yul Cha
Abstract: The present invention provides a process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonic stem cells, which comprises: (a) treating embryoid bodies differentiated from embryonic stem cells with 0.005-0.015% trypsin and 0.05-0.15 mM ethylenediaminetetraacetate (EDTA) to obtain vascular endothelial cell clusters; and (b) treating the vascular endothelial cell clusters with 0.1-0.5% trypsin and 0.5-2 mM EDTA so as to separate the vascular endothelial cell clusters into single cells.
Type:
Grant
Filed:
June 13, 2007
Date of Patent:
April 5, 2011
Assignees:
Chabio & Diostech Co., Ltd., College of Medicine Pochon Cha University Industry-Academic Cooperation Foundation
Inventors:
Hyung-Min Chung, Sung-Hwan Moon, Ju-Mi Kim, Soo-Hong Lee
Abstract: The present invention provides a process for differentiation of vascular endothelial progenitor cells from embryoid bodies derived from embryonic stem cells, the process comprising: (a) treating a culture medium comprising embryoid bodies derived from embryonic stem cells such that the concentration of oxygen dissolved in the culture medium is in the range of about 1 ppm to about 5 ppm; (b) culturing the culture medium prepared in step (a) in an incubator in which the oxygen (O2) tension is equal to or less than about 15% to differentiate the embryoid bodies into vascular endothelial progenitor cells; and (c) isolating the vascular endothelial progenitor cells from the culture medium obtained in step (b).
Type:
Application
Filed:
August 14, 2008
Publication date:
September 2, 2010
Applicants:
CHABIO & DIOSTECH CO., LTD., COLLEGE OF MEDICINE POCHON CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION,
Inventors:
Hyung-Min Chung, Ju-Mi Kim, Soo-Hong Lee, Sung-Hwan Moon
Abstract: The present invention relates to novel miRNA molecules, more particularly to novel miRNA molecules isolated from human embryonic stem cells. The miRNA molecules provided by the present invention can be usefully used as a molecular marker for early developmental stages of undifferentiated human embryonic stem cells. Also, the miRNA molecules of the present invention may play an important role in the regulation of mammalian embryonic stem cells. Therefore, the miRNA molecules can be usefully used for analyzing regulatory networks of human embryonic stem cells.
Type:
Grant
Filed:
December 15, 2004
Date of Patent:
March 9, 2010
Assignee:
College of Medicine, Pochon Cha University Industry -Academic Cooperation Foundation
Inventors:
Kye-Seong Kim, Mi-Ra Suh, Vit-Narry Kim
Abstract: The present invention relates to a method for culturing human embryonic stem cells (hESCs) in a hESC culture medium comprising a porous membrane, feeder cells being attached to a bottom of the porous membrane and a method for recovering human embryonic stem cells using the same.
Type:
Application
Filed:
May 31, 2007
Publication date:
May 21, 2009
Applicants:
CHABIOTECH CO., LTD., COLLEGE OF MEDICINE POCHON CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
Inventors:
Hyung-Min Chung, Soo-Hong Lee, Si-Nae Kim, Min-Jeong Kim