Patents Assigned to Charles River Laboratories
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Publication number: 20130316394Abstract: The invention relates to a method of detecting viable cells in a cell sample, using a membrane permeable fluorescent label that permeates both viable and non-viable cells and a membrane impermeant quencher that selectively permeates non-viable cells.Type: ApplicationFiled: May 2, 2013Publication date: November 28, 2013Applicant: Charles River Laboratories, Inc.Inventor: Charles River Laboratories, Inc.
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Publication number: 20130309686Abstract: The invention relates to a method of detecting viable cells in a cell sample, using a membrane permeable fluorescent label that permeates both viable and non-viable cells and a membrane impermeant quencher that selectively permeates non-viable cells.Type: ApplicationFiled: May 2, 2013Publication date: November 21, 2013Applicant: CHARLES RIVER LABORATORIES, INC.Inventor: CHARLES RIVER LABORATORIES, INC.
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Patent number: 8440394Abstract: The invention provides methods and compositions for the detection and/or quantification of a Gram positive bacterial contaminant in a sample. In particular, the invention provides hemocyte-based preparations, methods of making such hemocyte-based preparations, and methods of using such hemocyte-based preparations for the detection and/or quantification of the Gram positive bacterial contaminant.Type: GrantFiled: April 11, 2011Date of Patent: May 14, 2013Assignee: Charles River Laboratories, Inc.Inventors: Norman R. Wainwright, Foster T. Jordan
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Publication number: 20110201049Abstract: The invention provides methods and compositions for the detection and/or quantification of a microbial contaminant, for example, a bacterial endotoxin or a glucan, in a sample. In particular, the invention provides a test cartridge useful in the practice of hemocyte lysate-based assays for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides methods of making and using such cartridges. In addition, the invention provides a rapid, sensitive, multi-step kinetic hemocyte lysate-based assay for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides a glucan-specific lysate that can be used in a variety of assay formats, including, for example, a test cartridge, optionally configured to perform a kinetic assay.Type: ApplicationFiled: April 27, 2011Publication date: August 18, 2011Applicant: Charles River Laboratories, Inc.Inventors: Norman R. Wainwright, Foster T. Jordan
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Patent number: 7968280Abstract: The invention provides methods and compositions for the detection and/or quantification of a Gram positive bacterial contaminant in a sample. In particular, the invention provides hemocyte-based preparations, methods of making such hemocyte-based preparations, and methods of using such hemocyte-based preparations for the detection and/or quantification of the Gram positive bacterial contaminant.Type: GrantFiled: December 1, 2005Date of Patent: June 28, 2011Assignee: Charles River Laboratories, Inc.Inventors: Norman R. Wainwright, Foster T. Jordan
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Patent number: 7939291Abstract: The invention provides methods and compositions for the detection and/or quantification of a microbial contaminant, for example, a bacterial endotoxin or a glucan, in a sample. In particular, the invention provides a test cartridge useful in the practice of hemocyte lysate-based assays for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides methods of making and using such cartridges. In addition, the invention provides a rapid, sensitive, multi-step kinetic hemocyte lysate-based assay for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides a glucan-specific lysate that can be used in a variety of assay formats, including, for example, a test cartridge, optionally configured to perform a kinetic assay.Type: GrantFiled: August 1, 2007Date of Patent: May 10, 2011Assignee: Charles River Laboratories, Inc.Inventors: Norman R. Wainwright, Foster T. Jordan
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Patent number: 7901899Abstract: The invention provides a method using a hemocyte preparation, for example, Limulus amebocyte lysate, for detecting in a single assay the presence of at least one of a Gram negative bacterium, a Gram positive bacterium, and a fungus in a sample of interest. The method exploits the differential reactivity of Gram negative bacteria, Gram positive bacteria, and fungi with the hemocyte preparation to produce measurable changes in a property, for example, an optical property, of the mixture. Because the Gram negative bacteria, Gram positive bacteria and fungi each produce different changes in a given property, for example, an optical property, it is possible to classify the type of microorganism present in the sample of interest.Type: GrantFiled: October 23, 2008Date of Patent: March 8, 2011Assignee: Charles River Laboratories, Inc.Inventors: Norman R. Wainwright, Dana M. Nutter
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Publication number: 20100330597Abstract: The application provides heat-treated Limulus amebocyte lysates useful for detecting ?-glucans.Type: ApplicationFiled: June 25, 2010Publication date: December 30, 2010Applicant: Charles River Laboratories, Inc.Inventor: Masakazu Tsuchiya
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Patent number: 7479375Abstract: The invention provides a method using a hemocyte preparation, for example, Limulus amebocyte lysate, for detecting in a single assay the presence of at least one of a Gram negative bacterium, a Gram positive bacterium, and a fungus in a sample of interest. The method exploits the differential reactivity of Gram negative bacteria, Gram positive bacteria, and fungi with the hemocyte preparation to produce measurable changes in a property, for example, an optical property, of the mixture. Because the Gram negative bacteria, Gram positive bacteria and fungi each produce different changes in a given property, for example, an optical property, it is possible to classify the type of microorganism present in the sample of interest.Type: GrantFiled: January 13, 2006Date of Patent: January 20, 2009Assignee: Charles River Laboratories, Inc.Inventors: Norman R. Wainwright, Dana M. Nutter
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Patent number: 7329538Abstract: The invention provides methods and compositions for the detection and/or quantification of a microbial contaminant, for example, a bacterial endotoxin or a glucan, in a sample. In particular, the invention provides a test cartridge useful in the practice of hemocyte lysate-based assays for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides methods of making and using such cartridges. In addition, the invention provides a rapid, sensitive, multi-step kinetic hemocyte lysate-based assay for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides a glucan-specific lysate that can be used in a variety of assay formats, including, for example, a test cartridge, optionally configured to perform a kinetic assay.Type: GrantFiled: March 17, 2004Date of Patent: February 12, 2008Assignee: Charles River Laboratories, Inc.Inventors: Norman R. Wainwright, Foster T. Jordan, Frank C. Rumore, Charles Balas, Jr.
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Publication number: 20040241788Abstract: The invention provides methods and compositions for the detection and/or quantification of a microbial contaminant, for example, a bacterial endotoxin or a glucan, in a sample. In particular, the invention provides a test cartridge useful in the practice of hemocyte lysate-based assays for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides methods of making and using such cartridges. In addition, the invention provides a rapid, sensitive, multi-step kinetic hemocyte lysate-based assay for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides a glucan-specific lysate that can be used in a variety of assay formats, including, for example, a test cartridge, optionally configured to perform a kinetic assay.Type: ApplicationFiled: March 17, 2004Publication date: December 2, 2004Applicant: Charles River Laboratories, Inc.Inventors: Norman R. Wainwright, Foster T. Jordan, Frank C. Rumore, Charles Balas
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Patent number: 6725859Abstract: Apparatus and methods for delivery of air-borne materials from pulsatile delivery devices by passing the emissions from such devices and air in a coaxial manner through a suitable mixing chamber. Preferred apparatus include a chamber defining an axis of air flow, a plurality of pulsatile delivery devices, an actuator in communication with the devices for selective actuation thereof, and a device for introducing air at a first end of the chamber and for flowing that air substantially along the axis. The pulsatile delivery devices are positioned at the first end of the chamber such that actuation of the devices emits an air-borne substance substantially along the axis of air flow.Type: GrantFiled: January 14, 1998Date of Patent: April 27, 2004Assignee: Charles River LaboratoriesInventors: Simon J. Rothenberg, Hsu Chi Yeh, Bernard J. Greenspan
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Publication number: 20030104501Abstract: The invention provides methods and compositions for the detection and/or quantification of bacterial endotoxins. In particular, provided herein is an inexpensive and reproducible method for producing an improved amebocyte lysate preparation having reduced Factor G activity. Provided also is an endotoxin-specific amebocyte lysate preparation produced by such a method. In addition, the invention provides methods and compositions for enhancing the sensitivity to endotoxins of amebocyte lysate preparations having reducing Factor G activity. In particular, the sensitivity of such amebocyte lysate preparations to endotoxins can be enhanced by the addition of exogenous (1→3) &bgr;-D-glucan.Type: ApplicationFiled: April 26, 2002Publication date: June 5, 2003Applicant: Charles River LaboratoriesInventors: Foster T. Jordan, Hui-Ti Chiang, James F. Cooper, Norman R. Wainwright
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Patent number: 6391570Abstract: The invention provides methods and compositions for the detection and/or quantification of bacterial endotoxins. In particular, provided herein is an inexpensive and reproducible method for producing an improved amebocyte lysate preparation having reduced Factor G activity. Provided also is an endotoxin-specific amebocyte lysate preparation produced by such a method. In addition, the invention provides methods and compositions akin for enhancing the sensitivity to endotoxins of amebocyte lysate preparations having reducing Factor G activity. In particular, the sensitivity of such amebocyte lysate preparations to endotoxins can be enhanced by the addition of exogenous (1→3) &bgr;-D-glucan.Type: GrantFiled: September 18, 2000Date of Patent: May 21, 2002Assignee: Charles River LaboratoriesInventors: Foster T. Jordan, Hui-Ti Chiang, James F. Cooper, Norman R. Wainwright
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Patent number: 6270982Abstract: The invention provides methods and compositions for the detection and/or quantification of bacterial endotoxins. In particular, provided herein is an inexpensive and reproducible method for producing an improved amebocyte lysate preparation having reduced Factor G activity. Provided also is an endotoxin-specific amebocyte lysate preparation produced by such a method. In addition, the invention provides methods and compositions for enhancing the sensitivity to endotoxins of amebocyte lysate preparations having reducing Factor G activity. In particular, the sensitivity of such amebocyte lysate preparations to endotoxins can be enhanced by the addition of exogenous (1→3) &bgr;-D-glucan.Type: GrantFiled: October 9, 1997Date of Patent: August 7, 2001Assignee: Charles River LaboratoriesInventors: Foster T. Jordan, Hui-Ti Chiang, James F. Cooper, Norman R. Wainwright
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Patent number: 5370079Abstract: A container for transporting laboratory-type animals comprises a base and a lid, wherein the base and the lid when assembled define a volume for receiving one or more laboratory animals therewithin. The base includes a flange for contacting the lid, wherein the lid is removably attached to the flange of the base with removable stitching.Type: GrantFiled: May 3, 1993Date of Patent: December 6, 1994Assignee: Charles River LaboratoriesInventor: Roger W. Smith
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Patent number: D472324Type: GrantFiled: April 5, 2002Date of Patent: March 25, 2003Assignee: Charles River Laboratories, Inc.Inventors: Frank C. Rumore, Charles Balas, Jr.
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Patent number: D667022Type: GrantFiled: February 11, 2011Date of Patent: September 11, 2012Assignee: Charles River Laboratories, Inc.Inventors: Robert A. LoBosco, Yanning Zhu, Richard E. Long, Courosh Mehanian, Lucas C. Siler
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Patent number: D667417Type: GrantFiled: February 11, 2011Date of Patent: September 18, 2012Assignee: Charles River Laboratories, Inc.Inventors: Richard E. Long, Lucas C. Siler, Robert A. LoBosco, Yanning Zhu, Courosh Mehanian
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Patent number: D667418Type: GrantFiled: February 11, 2011Date of Patent: September 18, 2012Assignee: Charles River Laboratories, Inc.Inventors: Robert A. LoBosco, Courosh Mehanian, Lucas C. Siler, Yanning Zhu, Richard E. Long