Abstract: A system for measuring oxygen consumption uses a chamber holding culture medium with a biological material. A resistively gas-permeable membrane has a first side contacting culture medium in the chamber and a first sensor for measuring oxygen in the culture medium. A second sensor measures oxygen in gas adjacent a second side of the membrane. The sensors provide data to a processor having a memory with machine readable instructions for determining oxygen consumption from the data. Alternatively, a method of measuring oxygen flux begins with placing biological material, with fluid, in the chamber; exposing the fluid to a resistively-permeable membrane with oxygen-containing gas on a second side of the membrane; measuring oxygen in the fluid; measuring oxygen in the gas, and calculating the oxygen flux of the biological material from an oxygen permeability constant of the membrane and a difference between the oxygen concentrations of the fluid and gas.
Abstract: Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a nucleic acid encoding a mutant von Willebrand Factor protein to correct a point mutation associated with a disease or disorder, e.g., with von Willebrand disease. The methods provided are useful for correcting a vWF point mutation within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins of Cas9 and nucleic acid editing enzymes or enzyme domains, e.g., deaminase domains, are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of Cas9 and nucleic acid editing enzymes or domains, are provided.
Type:
Application
Filed:
July 8, 2014
Publication date:
June 18, 2015
Applicant:
President and Fellows of Harvard College
Abstract: Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins of Cas9 and nucleic acid editing enzymes or enzyme domains, e.g., deaminase domains, are provided. In some embodiments, methods for targeted nucleic acid editing are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of Cas9 and nucleic acid editing enzymes or domains, are provided.
Type:
Application
Filed:
July 8, 2014
Publication date:
June 18, 2015
Applicant:
President and Fellows of Harvard College
Abstract: A computing device determines a first table included in a plurality of tables, wherein the plurality of tables are included in the database. The computing device determines a dependency corresponding to the first table, wherein the dependency identifies a second table that is included in the plurality of tables. The computing device determines a distribution corresponding to the dependency, wherein the distribution identifies a correlation corresponding to the first table and to the second table. The computing device analyzes the correlation to determine a group of data values of the first table and the second table. The computing device selects a subset of data values from the group of data values. The computing device populates a sample with the subset.
Type:
Application
Filed:
December 18, 2013
Publication date:
June 18, 2015
Applicants:
University College Dublin, International Business Machines Corporation
Inventors:
Teodora S. Buda, Morten K. Kristiansen, Nirmala Venkatraman
Abstract: The present invention provides novel moenomycin analogs as well as pharmaceutical compositions thereof, methods of synthesis, and methods of use in treating an infection by administering an inventive compound to a subject in need thereof. The moenomycin analogs may be prepared synthetically, biosynthetically, or semi-synthetically. The analogs are particularly useful in treating or preventing infections caused by Gram-positive organisms. Certain inventive compounds may have a broader spectrum of coverage, which includes Gram-negative organisms.
Type:
Application
Filed:
December 9, 2013
Publication date:
June 18, 2015
Applicant:
President and Fellows of Harvard College
Inventors:
Daniel Evan Kahne, Suzanne Walker Kahne, Masaatsu Adachi, Emma Doud, Shinichiro Fuse, Xiaonan Lin, Yi Zhang, Hirokazu Tsukamoto, Bohdan Omelyanovich Ostash
Abstract: Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a nucleic acid encoding a mutant Caspase-9 protein to correct a point mutation associated with a disease or disorder, e.g., with neuroblastoma. The methods provided are useful for correcting a Caspase-9 point mutation within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins of Cas9 and nucleic acid editing enzymes or enzyme domains, e.g., deaminase domains, are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of Cas9 and nucleic acid editing enzymes or domains, are provided.
Type:
Application
Filed:
July 8, 2014
Publication date:
June 18, 2015
Applicant:
President and Fellows of Harvard College
Abstract: Methods of fabricating micro- and nanostructures comprise top-down etching of lithographically patterned GaN layer to form an array of micro- or nanopillar structures, followed by selective growth of GaN shells over the pillar structures via selective epitaxy. Also provided are methods of forming micro- and nanodisk structures and microstructures formed from thereby.
Type:
Application
Filed:
December 12, 2014
Publication date:
June 18, 2015
Applicants:
University of Maryland, College Park, Northrop Grumman Systems Corporation, United States of America, as Represented by the Secretary of Commerce
Inventors:
Abhishek Motayed, Sergiy Krylyuk, Albert V. Davydov, Matthew King, Jong-Yoon Ha
Abstract: Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a nucleic acid encoding a mutant Presenilin1 protein to correct a point mutation associated with a disease or disorder, e.g., with familial Alzheimer's disease. The methods provided are useful for correcting a PSEN1 point mutation within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins of Cas9 and nucleic acid editing enzymes or enzyme domains, e.g., deaminase domains, are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of Cas9 and nucleic acid editing enzymes or domains, are provided.
Type:
Application
Filed:
July 8, 2014
Publication date:
June 18, 2015
Applicant:
President and Fellows of Harvard College
Abstract: Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins of Cas9 and nucleic acid editing enzymes or enzyme domains, e.g., deaminase domains, are provided. In some embodiments, methods for targeted nucleic acid editing are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of Cas9 and nucleic acid editing enzymes or domains, are provided.
Type:
Application
Filed:
July 8, 2014
Publication date:
June 18, 2015
Applicant:
President and Fellows of Harvard College
Abstract: The invention provides methods, cells and constructs for optical measurement of membrane potential. These methods can be used in cells that are not accessible to presently available methods using electrodes. The methods can be directed to, for example, high-throughput drug screening assays to determine agents that can affect membrane potential of a target cell.
Type:
Grant
Filed:
August 23, 2011
Date of Patent:
June 16, 2015
Assignee:
President and Fellows of Harvard College
Inventors:
Adam E. Cohen, Joel M. Kralj, Adam D. Douglass
Abstract: An artificial composite object combines a quantum of sound with a matter excitation. A phonon in a confinement structure containing the matter excites it from an initial state to an excited state corresponding to a frequency of the phonon. Relaxation of the matter back to the initial state emits a phonon of the same frequency into the confinement structure. The phonon confinement structure, for example, a cavity, traps the emitted phonon thereby allowing further excitation of the matter. The coupling between the phonon and the matter results in a quantum quasi-particle referred to as a phoniton. The phoniton can find application in a wide variety of quantum systems such as signal processing and communications devices, imaging and sensing, and information processing.
Type:
Grant
Filed:
March 15, 2013
Date of Patent:
June 16, 2015
Assignees:
University of Maryland College Park, The United States od America, as represented by the Director, National Security Agency
Inventors:
Charles George Tahan, Rousko Todorov Hristov, Oney O. Soykal
Abstract: Parallel uses of microfluidic methods and devices for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid are described. In some aspects, the present invention relates generally to flow-focusing-type technology, and also to microfluidics, and more particularly parallel use of microfluidic systems arranged to control a dispersed phase within a dispersant, and the size, and size distribution, of a dispersed phase in a multi-phase fluid system, and systems for delivery of fluid components to multiple such devices.
Type:
Grant
Filed:
March 12, 2010
Date of Patent:
June 16, 2015
Assignee:
President and Fellows of Harvard College
Inventors:
Mark Romanowsky, Adam R. Abate, David A. Weitz
Abstract: The present invention is generally related to systems and methods for producing droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, at least one droplet is used to create a plurality of droplets, using techniques such as flow-focusing techniques. In one set of embodiments, a plurality of droplets, containing varying species, can be divided to form a collection of droplets containing the various species therein. A collection of droplets, according to certain embodiments, may contain various subpopulations of droplets that all contain the same species therein. Such a collection of droplets may be used as a library in some cases, or may be used for other purposes.
Type:
Grant
Filed:
October 26, 2010
Date of Patent:
June 16, 2015
Assignee:
President and Fellows of Harvard College
Abstract: The present invention generally relates to systems and methods for treating certain oxidative stress conditions. In one aspect, compositions and methods of the invention can be used to treat a subject having an oxidative stress condition, for example, a subject having pulmonary fibrosis. In some embodiments, an inhibitor of ERp57 (for example, thiomuscimol) and/or an inhibitor of GSTP (for example, TLK-199) may be used to treat the subject. Also provided in certain aspects of the present invention are kits for such therapies, methods for promoting such therapies, and the like.
Type:
Application
Filed:
June 20, 2013
Publication date:
June 11, 2015
Applicant:
The University of Vermont and State Agricultural College
Inventors:
Yvonne M. Janssen-Heininger, Vikas Anathy
Abstract: A method, computer-readable storage device and apparatus for calculating a health quality measure are disclosed. For example, a method receives characteristics of motion information, wherein the characteristics of motion information is based upon gait information, monitors the characteristics of motion information over a time period to determine a plurality of different modes of motion within the time period, and calculates the health quality measure based upon the plurality of different modes of motion.
Type:
Application
Filed:
December 6, 2013
Publication date:
June 11, 2015
Applicants:
President and Fellows of Harvard College, AT&T Intellectual Property I, L.P.
Inventors:
Saeed S. Ghassemzadeh, Lusheng Ji, Robert Raymond Miller, II, Manish Gupta, Vahid Tarokh