Abstract: A filament assembly can include: a button having a planar emitter region with one or more apertures extending from an emission surface of the planar emitter region to an internal surface opposite of the emission surface; an inlet electrical lead coupled to the button at a first side; an outlet electrical lead coupled to the button at a second side opposite of the first side; and a low work function object positioned adjacent to the internal surface of the planar emitter region and retained to the button. The planar emitter region can include a plurality of apertures. The low work function object can include a porous ceramic material having the barium, and may have a polished external surface. An electron gun can include the filament assembly. An additive manufacturing system can include the electron gun having the filament assembly.

Abstract: A bistable gripper for an aerial vehicle may include a base, a first finger, a second finger, a switching pad, a first elastic connector, and a second elastic connector. The base may include a first beam and a second beam. The first finger may be pivotably attached to the first beam at a first joint, and the second finger may be pivotably attached to the second beam at a second joint. The switching pad may be configured for moving relative to the base. The first elastic connector may be attached to the switching pad and the first finger, and the second elastic connector may be attached to the switching pad and the second finger. The bistable gripper may be configured for switching between a closed stable state and an open stable state.

Abstract: A new solvent-based method is presented for making low-cost composite graphite electrodes containing a thermoplastic binder. The electrodes, termed thermoplastic electrodes (TPEs), are easy to fabricate and pattern, give excellent electrochemical performance, and have high conductivity (1500 S m?1). The thermoplastic binder enables the electrodes to be hot embossed, molded, templated, and/or cut with a CO2 laser into a variety of intricate patterns. These electrodes show a marked improvement in peak current, peak separation, and resistance to charge transfer over traditional carbon electrodes. The impact of electrode composition, surface treatment (sanding, polishing, plasma treatment), and graphite source were found to impact fabrication, patterning, conductivity, and electrochemical performance. Under optimized conditions, electrodes generated responses similar to more expensive and difficult to fabricate graphene and highly oriented pyrolytic graphite electrodes.

Abstract: The invention discloses a class of new polymers, trans-ring-fused poly(4-hydroxybutyrate)s (RF-P4HB) that exhibit a unique set of properties, including robust thermal stability and mechanical strength, quantitative recyclability to the building block monomers via thermolysis and/or chemical catalysis, and convenient production from the chemical ring-opening polymerization under ambient temperature and pressure. Another unique property is the formation of crystalline stereocomplexed polymers with high melting temperature upon mixing the two enantiomeric RF-P4HB chains via stereocomplexing co-crystallization. This invention also provides the corresponding ring-fused lactone monomer structures that enable the synthesis of the RF-P4HB polymers, through trans-fusing of rings to the parent ?-butyrolactone ring. Furthermore, a polymerization or copolymerization process for the synthesis of RF-P4HB polymers and copolymers is disclosed.

Abstract: The present invention provides efficient processes for preparing brush polymers. In general, the process comprises three distinct reaction steps utilizing two separate catalysts. In the first step, the initiating compound comprising norbornene is contacted with a silane in the presence of a catalyst, thereby forming a silated intermediate. This silated intermediate is then contacted with a monomer in the presence of a catalyst via Group Transfer Polymerization (GTP). The resulting compound from GTP is contacted with a ring opening metathesis polymerization (ROMP) catalyst to prepare the brush polymer. Surprisingly, the brush polymers obtained from the above process are accessed in an efficient and rapid GTP methodology as compared to prior methods.

Abstract: A novel technique for label-free, rapid detection of ultra-low concentrations of virus specific antibodies is described. We have developed a simple, robust capacitive biosensor using microwires coated with Zika or Chikungunya virus envelope antigen. With little discernable nonspecific binding, the sensor can detect as few as 10 antibody molecules in a small volume (10 molecules/30 ?L) within minutes. It can also be used to rapidly, specifically, and accurately determine the isotype of antigen-specific antibodies. Finally, we demonstrate that anti-Zika virus antibody can be sensitively and specifically detected in dilute mouse serum and can be isotyped using the sensor. Overall, our findings indicate that our microwire sensor platform can be used as a reliable, sensitive, and inexpensive diagnostic tool to detect immune responses at the point of care.

Abstract: Disclosed are yeast-based immunotherapeutic compositions and fusion proteins for the treatment and/or prevention of TB infection and symptoms thereof, as well as methods of using the yeast-based immunotherapeutic compositions and fusion proteins for the prophylactic and/or therapeutic treatment of TB and/or symptoms thereof.

Abstract: The present disclosure provides co-cultures of human pluripotent stem cell derived hepatocytes and at least one non-parenchymal cell population in vitro, methods of preparing the co-cultures and methods of using the co-cultures for high throughput screening and evaluation of drug candidates. The stem cell derived hepatocyte co-culture system provides an in vitro model in which cell viability and relatively mature hepatocyte phenotype of stem cell derived hepatocytes are maintained for extended periods relative to conventional monoculture.

Abstract: The present disclosure relates to consortia of bacteria strains and composition comprising one or more bacterial strains disclosed herein. These consortia of isolated bacteria cultures and compositions comprising said cultures, having greater activity than would be observed for the individual bacteria cultures and compositions. A composition of the disclosure may advantageously be used for enhancing the availability of soil phosphorus and other macronutrients and/or micronutrients to plants, and thereby enhancing their growth and yield.

Abstract: A photonic-crystal-fiber-delivered laser-triggered high-voltage gas switch can deliver a peak irradiance of greater than 5.1×1011 W/cm2 to the AK gap for a laser having a wavelength of 1064 nm. The switch is capable of operating at pressures up to 2200 psi; voltages across the gap of greater than 200 kV; operation at less than 70% self-break voltage; shot-to-shot jitter of less than 3 ns; AK gap distances of 3 mm or smaller; and triggering via a fiber-delivered laser pulse energy of as low as 500 ?J.

Abstract: According to some embodiments, the present disclosure may relate to a system including a gaseous fuel consuming engine operating at an air to fuel ratio (AFR) and including a throttle valve controlling a speed of engine, and an engine controller coupled to the engine. The engine controller may be configured to obtain the speed of the engine and obtain the AFR of the engine. The engine controller may also be configured to, based on a transient event affecting the engine, coordinate modification of both the throttle valve to change the speed of the engine and trim valve to change the AFR of the engine to maintain at least one of the speed and the AFR of the engine within a threshold deviance.

Abstract: The disclosure encompasses resurfaced cell-penetrating nanobodies and their methods of use. The resurfacing of nanobodies with positively-charged amino acids facilitates their penetration into a cell and allows targeting of a specific intracellular protein.

Abstract: A new solvent-based method is presented for making low-cost composite graphite electrodes containing a thermoplastic binder. The electrodes, termed thermoplastic electrodes (TPEs), are easy to fabricate and pattern, give excellent electrochemical performance, and have high conductivity (1500 S m?1). The thermoplastic binder enables the electrodes to be hot embossed, molded, templated, and/or cut with a CO2 laser into a variety of intricate patterns. These electrodes show a marked improvement in peak current, peak separation, and resistance to charge transfer over traditional carbon electrodes. The impact of electrode composition, surface treatment (sanding, polishing, plasma treatment), and graphite source were found to impact fabrication, patterning, conductivity, and electrochemical performance. Under optimized conditions, electrodes generated responses similar to more expensive and difficult to fabricate graphene and highly oriented pyrolytic graphite electrodes.

Abstract: Analogs of largazole are described herein. Methods of treating cancer and blood disorders using largazole and largazole analogs and pharmaceutical compositions comprising the same are additionally described herein. Methods for preparing largazole analogs are likewise described.

Abstract: A loading device, a monitoring system, and a method thereof can measure stiffness of a structural member (SM), such as a bone, and monitor progress or property over time. The loading device includes two types of displacement sensors, one type being an antenna. As the SM, which is in a magnetic or electromagnetic field and electromagnetically coupled to the antenna without contact, undergoes displacement under known loads, characteristics of the electromagnetic field coupling between the antenna and the SM change over time due to the displacement of the SM. The shift in the characteristics of the electromagnetic field coupling between the antenna and the SM can be used to determine the displacement of the SM. Based on the changes in the displacement over time, diagnosis of the SM being monitored over an evaluation period can be made.

Abstract: The present disclosure provides methods for detecting early Lyme disease. The present disclosure provides a biosignature indicative of the presence or absence of Borrelia burgdorferi infection.

Abstract: The disclosure relates to a dual catalytic method for forming aryl carbon-nitrogen bonds. The method comprises contacting an aryl halide with an amine in the presence of a dual catalytic solution comprising a Ni(II) salt catalyst, a photocatalyst, and an optional base, thereby forming a reaction mixture; exposing the reaction mixture to light under reaction condition sufficient to produce the aryl carbon-nitrogen bonds. In certain embodiments, the amine may be present in a molar excess to the aryl halide. In certain embodiment, the photocatalyst may be [Ru(bpy)3]Cl2 or an organic phenoxazine. In certain embodiments, the Ni salt catalyst solution includes a Ni(II) salt and a polar solvent, wherein the Ni(II) salt is dissolved in the polar solvent.

Abstract: 2-Aminothiophene derivatives, uses of the same, and methods of making the same, are described.

Abstract: The present disclosure provides a biosignature that distinguishes Lyme disease, including early Lyme disease, from STARI. The present disclosure also provides methods for detecting Lyme disease and STARI, as well as methods for treating subjects diagnosed with Lyme disease or STARI.

Abstract: A monitoring method and system include an antenna disposed spaced from a structural member (SM), which itself can be the target object or attachable to a target object, without using any in-dwelling strain sensor. The antenna is arranged to not touch the SM in at least the no load condition. As the target object undergoes displacement and/or deformation, the SM undergoes displacement and/or deformation. The SM is juxtaposed, partially contained with, or fully contained within a magnetic or electromagnetic field and electromagnetically coupled to the emitting antenna. Characteristics of the electromagnetic field coupling between the antenna and the SM shift over time due to the displacement and/or deformation applied to the SM. The shift in the characteristics of the electromagnetic field coupling between the antenna and the SM over time can be used to determine the temporal change in deformation and/or displacement of the SM over time to enable diagnosis of the target structural object being monitored.