Abstract: This disclosure is related to a method of sequencing a single-stranded DNA using deoxynucleotide polyphosphate analogues and translocation of tags from incorporated deoxynucleotide polyphosphate analogues through a nanopore.

Abstract: Media for making esophageal organoids, methods of making an esophageal organoid using that media, and esophageal organoids produced by those methods

Abstract: Optical imaging or spectroscopy described can use laminar optical tomography (LOT), diffuse correlation spectroscopy (DCS), or the like. An incident beam is scanned across a target. An orthogonal or oblique optical response can be obtained, such as concurrently at different distances from the incident beam. The optical response from multiple incident wavelengths can be concurrently obtained by dispersing the response wavelengths in a direction orthogonal to the response distances from the incident beam. Temporal correlation can be measured, from which flow and other parameters can be computed. An optical conduit can enable endoscopic or laparoscopic imaging or spectroscopy of internal target locations. An articulating arm can communicate the light for performing the LOT, DCS, or the like. The imaging can find use for skin cancer diagnosis, such as distinguishing lentigo maligna (LM) from lentigo maligna melanoma (LMM).

Abstract: Provided herein are methods and related devices for preprocessing a biological sample during transit. The method may comprise the steps of: storing a biological sample in a storage container having walls that defines a storage volume; transporting the storage container with the stored biological sample to a sample processing facility; controlling one or more storage container parameters during the transporting step to initiate preprocessing of the biological sample; wherein the controlling step improves a processing parameter at the sample processing facility.

Abstract: Devices for radiative cooling and optical waveguiding are provided, wherein the devices comprise a fabric including one or more fibers extending for a length in a longitudinal direction and a plurality of void structures positioned within each of the one or more fibers and extended over the length of each of the one or more fibers. Each of the plurality of void structures is configured to scatter at least a portion of an electromagnetic radiation received thereon to thereby radiatively cool the object.

Abstract: Mechanisms for defending a computing system from attack are provided. The mechanisms include: maintaining a round counter that tracks a round number for a local host; determining a location in a graph for each of a plurality of hosts including the local host; determining monitor hosts of the plurality of hosts that are monitoring the local host; determining monitoree hosts of the plurality of hosts that are being monitored by the local host; sending a message to each of the monitor hosts identifying a value of the round counter; forwarding a first set of heartbeat messages from previous monitoree hosts to the monitor hosts; attempting to receive messages from the monitoree hosts; determining whether any messages were not received from the monitoree hosts; and in response to determining that one or more messages were not received from the monitoree hosts, generating an alert.

Abstract: Examples of syringe assemblies and sets of components are disclosed for transferring a fluid sample for testing. In some examples, the syringe assembly comprises a housing, a barrel, a plunger reciprocally moveable in the barrel, a narrow tube connected to the barrel, and a metering actuator configured to control movement of the plunger at least in a proximal direction relative to the barrel. The metering actuator is configured to draw a precise microvolume of the fluid sample into a fluid chamber within the syringe assembly. In some examples, while the fluid chamber holds the fluid sample, the distal tip of the narrow tube remains covered, for example inside the housing or a reaction container. The syringe assembly is configured to expel the fluid sample from the fluid chamber into the reaction container.

Abstract: Exemplary methods, compositions and uses thereof can be provided for preventing, reducing and/or treating loss of muscle function. In particular, e.g., it is possible to administer to a subject a pharmaceutical composition comprising a therapeutically effective amount of an agent that enhances Interleukin-6 (IL) release during exercise, and optionally a pharmaceutically acceptable carrier or excipient.

Abstract: Systems and methods of performing temperature swing solvent extraction (TSSE) descaling of produced water and desalination of high-salinity brines, e.g., those having a total dissolved solids (TDS) greater than about 250,000 ppm are capable of producing descaled water products including less than about 5% weight percent TDS. The brine/produced water feedstreams and combined with a solvent having temperature-dependent water solubility at a temperature TL. Water is extracted from the feedstream into the solvent to form a water-in-solvent extract component and a raffinate component, from which a solid phase can be precipitated as more water is portioned in the solvent and basicity increases. Heating of the water-in-solvent extract component reduces the solubility of the water therein, producing a biphasic mixture of dewatered solvent and descaled water that can be separated.

Abstract: The present inhibitory polypeptides/peptides are derived from an envelope (E) protein of a coronavirus (e.g., human coronaviruses such as SARS-CoV-2). The polypeptides/peptides against coronaviruses can be used to treat or prevent infection by a coronavirus in a subject.

Abstract: Composites, are provided, the composites comprising: mixed conducting particles; and an ion conducting scaffolding matrix. In some embodiments, the mixed conducting particles are made from poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate). In some embodiments, the ion conducting scaffolding matrix includes a chitosan (CS)-based polymer. In some embodiments, devices are provided, the devices comprising: a composite comprising mixed conducting particles and an ion conducting scaffolding matrix; and three electrodes, wherein: each of the three electrodes is in contact with the composite; a first pair of the three electrodes are on opposite sides of the composite and are a distance h apart; a second pair of the three electrodes are on a same side of the composite and are a distance d1 apart; a particle size of the mixed conducting particles is between h and d1; a mean-free-path of the mixed conducting particles is less than d1; and the composite behaves like an anisotropic conductor.

Abstract: Multiple fluorophores within a sample can be imaged by merging a plurality of beams from different wavelength light sources of excitation light into a single path, directing the excitation light into the sample, and detecting light emitted by the fluorophores within the sample on two different arrays of pixels (e.g., two regions within a single camera sensor chip). The light sources are activated during respective timeslots, and captured image data is processed. For at least one of the timeslots, the processing of the image data comprises using the image data captured using the first array of pixels to detect a presence of a given fluorophore, and using the image data captured using the second array of pixels to detect a presence of a different fluorophore. This arrangement enables a system that includes only N light sources to image more than N fluorophores.

Abstract: Circuits and methods for multi-phase clock generators and phase interpolators are provided. The multi-phase clock generators include a delay line and multi-phase injection locked oscillator. At each stage of the multi-phase injection locked oscillator, injection currents are provided from a corresponding stage of the delay line. Outputs of the multi-phase injection locked oscillator and provided to mixers which produce inputs to an operational transconductance amplifier which provides feedback to the delay line and the multi-phase injection locked oscillator. The phase interpolator uses a technique of flipping certain input clock signals to reduce the number of components required while still being able to interpolate phase over 360 degrees and to reduce noise.

Abstract: The disclosed subject matter relates to a device, system and method for locating and repairing a pulmonary air leak in a patient.

Abstract: The disclosed subject matter relates to a cloud-accessible quantum simulator based on programmable atom arrays. An example cloud-accessible quantum simulator can include an atomic platform, a laser and photonics system, a timing and control box, a user interface, and quantum algorithms. The disclosed system provides a platform for developing and implementing quantum algorithms in multiple fields.

Abstract: Encoding environmental parameters by modulating swarm patterns of bacteria on substrates and correlating spatiotemporal changes in the environmental parameters to changes in swarm patterns using one or more trained machine learning models.

Abstract: Systems and methods of performing temperature swing solvent extraction (TSSE) descaling of produced water and desalination of high-salinity brines, e.g., those having a total dissolved solids (TDS) greater than about 250,000 ppm are capable of producing descaled water products including less than about 5% weight percent TDS. The brine/produced water feedstreams and combined with a solvent having temperature-dependent water solubility at a temperature TL. Water is extracted from the feedstream into the solvent to form a water-in-solvent extract component and a raffinate component, from which a solid phase can be precipitated as more water is portioned in the solvent and basicity increases. Heating of the water-in-solvent extract component reduces the solubility of the water therein, producing a biphasic mixture of dewatered solvent and descaled water that can be separated.

Abstract: In one aspect the present invention is directed to mutant NGAL proteins that have the ability to bind to siderophores, such as enterochelin, and to chelate and transport iron, and that are excreted in the urine. Such NGAL mutants, and complexes thereof with siderophores, can be used to clear excess iron from the body, for example in the treatment of iron overload. The NGAL mutants of the invention also have antibacterial activity and can be used in the treatment of bacterial infections, such as those of the urinary tract.

Abstract: An animal positioned on a perforated platform can be stimulated in an automated fashion. Images of the animal's paw are captured using a bottom-view camera positioned below the platform. Based on these images, an upwards-pointing tool is moved until it is directly beneath the paw. The animal is then stimulated automatically by moving the tool upwards to contact the paw, then moving it back down to cease the contact. Images of the animal are captured using at least one side-view camera, and these images can be analyzed to ascertain the animal's response to the stimulus.

Abstract: The disclosed subject matter relates to a photonically integrated atomic tweezer clock. An example atomic tweezer clock can include a laser system, a holographic metasurface, a vacuum system, and a cold atoms source, wherein the holographic metasurface generates an optical tweezer array, and the atoms are trapped by the optical tweezer array in the vacuum system for generating an atomic tweezer clock. In certain embodiments, the laser system is integrated with frequency combs in chip-scale to ensure compactness and robustness.