Abstract: The present invention relates to the use of non-maltogenic exoamylases of retarding the detrimental retrogradation of starch. Furthermore, the invention relates to a novel non-maltogenic exoamylase from Bacillus Clausii.
Type:
Application
Filed:
September 25, 2003
Publication date:
March 4, 2004
Applicant:
Danisco A/S
Inventors:
Karsten M. Kragh, Bjarne Larsen, Preben Rasmussen, Lene Duedahl-Olesen, Wolfgang Zimmermann
Abstract: The present invention relates to a process for making a bread product. The process includes the addition of a non-maltogenic exoamylase that hydrolyses starch to a starch medium, and the application of heat to the starch medium. The non-maltogenic exomylase cleaves one or more linear malto-oligosaccharides, predominantly consisting of from four to eight D-glucopyranosyl units, from non-reducing ends of amylopectin side chains. The non-maltogenic exoamylase has an endoamylase activity of less than 0.5 endoamylase units (EAU) per unit of exoamylase activity.
Type:
Grant
Filed:
February 28, 2001
Date of Patent:
December 23, 2003
Assignee:
Danisco A/S
Inventors:
Karsten M. Kragh, Bjarne Larsen, Preben Rasmussen, Lene Duedahl-Olesen, Wolfgang Zimmermann
Abstract: A method of preparing the sugar 1,5-D-anhydrofructose is described. The method comprises treating an &agr;-1,4-glucan with an &agr;-1,4-glucan lyase wherein the enzyme is used in substantially pure form. In a preferred embodiment, if the glucan contains links other than and in addition to the &agr;-1,4-links, the &agr;-1,4-glucan lyase is used in conjunction with a suitable reagent that can break the other links.
Type:
Application
Filed:
May 30, 2003
Publication date:
November 6, 2003
Applicant:
DANISCO A/S
Inventors:
Shukun Yu, Kirsten Bojsen, Karsten Kragh, Maja Bojko, John Nielsen, Jan Marcussen, Tove Christensen
Abstract: The present invention provides a method for producing a secretable polypeptide in a host cell. In the method, a peptidyl prolyl cis-trans isomerase is overexpressed in a host cell, thereby increasing the yield of the secreted polypeptide.
Abstract: A method of improving the Theological and/or machineability properties of a flour dough and/or the quality of the product made from the dough, comprising adding to the dough a combination comprising a Hox and an emulsifying agent.
Type:
Application
Filed:
October 1, 2002
Publication date:
October 16, 2003
Applicant:
Danisco A/S
Inventors:
Torkil Steenholt Olsen, Inge Lise Povlsen, Jorn Borch Soe, Charlotte Horsmans Poulsen, Pernille Bak Hostrup
Abstract: A method of preparing the sugar 1,5-D-anhydrofructose is described. The method comprises treating an &agr;-1,4-glucan with an &agr;-1,4-glucan lyase wherein the enzyme is used in substantially pure form. In a preferred embodiment, if the glucan contains links other than and in addition to the &agr;-1,4-links, the &agr;-1,4-glucan lyase is used in conjunction with a suitable reagent that can break the other links.
Type:
Grant
Filed:
March 29, 1999
Date of Patent:
October 14, 2003
Assignee:
Danisco A/S
Inventors:
Shukun Yu, Kirsten Bojsen, Karsten Kragh, Maja Bojko, John Nielsen, Jan Marcussen, Tove Christensen
Abstract: A process for treating a pectin with a pectin methyl esterase (PME) is descibed. Here, the PME is not a plant PME—but the PME is capable of exhibiting at least one plant PME property; wherein the at least one plant PME property comprises at least block—wise de-esterification of the pectin.
Type:
Grant
Filed:
July 16, 2001
Date of Patent:
September 30, 2003
Assignee:
Danisco A/S
Inventors:
Tove Martel Ida Else Christensen, Anette Amstrup Pedersen, Janne Brunstedt, Jorn Dalgaard Mikkelsen
Abstract: The present invention provides a method for producing a secretable polypeptide in a host cell. In the method, a peptidyl prolyl cis-trans isomerase is overexpressed in a host cell, thereby increasing the yield of the secreted polypeptide.
Abstract: The subject invention lies in the field of microorganism mutation and selection of the mutants. In particular, the invention is directed at obtaining metabolic mutants in a simple, direct and specific manner. In a preferred embodiment it is also possible to obtain desired mutants not comprising recombinant DNA, thereby facilitating incorporation thereof in products for human consumption or application, due to shorter legislative procedures. The method according to the invention involves random mutation and specific selection of the desired metabolic mutant. Knockout mutants wherein a gene associated with metabolism is absent or inactivated and mutants with increased or decreased DNA binding capacity are also claimed.
Type:
Grant
Filed:
July 11, 2000
Date of Patent:
July 29, 2003
Assignee:
Danisco Ingredients A/S (Danisco A/S)
Inventors:
Leendert Hendrik De Graaff, Henriëtta Catharina Van Den Broeck, Jacob Visser
Abstract: Recombinantly produced &agr;-glucuronidases which are useful in food manufacturing and as a feed additive to enhance the utilization of the feed components, and in other industrial applications such as pulp processing are provided. Genes coding for such enzymes can e.g. be isolated from Aspergillus sp. including A. tubigensis or A. niger.
Type:
Grant
Filed:
November 10, 1998
Date of Patent:
May 6, 2003
Assignee:
Danisco A/S
Inventors:
Charlotte Horsmans Poulsen, Masoud R. Zargahi, Ronald Peter de Vries, Jacob Visser
Abstract: A method of preparing 1,5-D-anhydrofructose in large quantities includes treating &agr;-1,4-glucan with a substantially pure &agr;-1,4-glucan lyase, which has been isolated from algae alone, wherein 1,5-D-anhydrofructose is produced directly from the &agr;-1,4-glucan.
Type:
Grant
Filed:
March 24, 1999
Date of Patent:
April 1, 2003
Assignee:
Danisco A/S
Inventors:
Shukun Yu, Kirsten Bojsen, Jan Marcussen
Abstract: There is provided use of a pectin composition to prepare an aqueous composition having high yield stress characteristics without substantial gelation, wherein the pectin composition comprises at least a population of pectin that is calcium sensitive; wherein the pectin composition is capable of imparting high yield stress characteristics to an aqueous composition without causing substantial gelation of the aqueous composition.
Abstract: There is provided a composition comprising (a) an oil and/or an oil mimetic component; (b) a triglyceride fatty acid and/or a high melting point emulsifier component; (c) a particulate component; wherein the particulate component (c) is in a stable suspension within a crystal matrix formed by component (b); with the proviso that the particulate component (c) does not form a crystal matrix.
Type:
Grant
Filed:
April 9, 1999
Date of Patent:
February 25, 2003
Assignee:
Danisco A/S
Inventors:
Bent Kvist Vogensen, Hanne Valsted Thygesen, Jørn Borch Søe
Abstract: The present invention is concerned with the use of the sequence CGGCAGGGTCTC, or a variant, homologue or fragment thereof, as a control element for activating transcription of a nucleotide sequence or nucleotide sequences from a promoter. The invention accordingly provides nucleic acid constructs in which at least one heterologous copy of the element is operatively liniked upstream or downstream of a promoter which is itself operatively linked to a nucleotide sequence.
Abstract: A method for affecting enzymatic activity in a starch producing organism is described. The method comprises expressing in the organism: (a) a first nucleotide which comprises, partially or completely, a first intron of a gene encoding a class A starch branching enzyme in an antisense orientation, wherein the first nucleotide sequence does not contain a sequence that is antisense to an exon sequence naturally associated with the first intron, (b) together with a second nucleotide sequence which comprises, partially or completely, a second intron of a class B starch branching enzyme in an antisense or sense orientation. Also described are antisense sequences, constructs, vectors, transformed cells, and transgenic organisms.
Abstract: A process is provided for separating essential oils from an essential oil-containing material. The process comprises the steps of subjecting the essential oil-containing material to steam distillation using a steam distillation vessel or extraction using an extraction vessel. This produces a mixture containing the essential oil and at least one hydrophilic phase. This mixture is then contacted with a hydrophobic adsorbent in an adsorption vessel. The hydrophilic phase leaving the adsorption vessel is recycled to the steam distillation vessel or extraction vessel. Finally, the essential oils are desorbed from the adsorbent.
Abstract: The present invention provides a method for producing a secretable polypeptide in a host cell, comprising overexpressing a peptidyl proly cis-trans isomerase in the host cell, thereby increasing the yield of the secreted polypeptide, and a novel peptidyl proly cis-trans isomerase from A. niger useful in such a method.
Abstract: A selection method for selecting from a population of plant cells one or more genetically transformed plant cells is described. In the method, the population of plant cells includes selectable genetically transformed plant cells and possible non-transformed plant cells. Each of the selectable genetically transformed plant cells comprises a first expressible nucleotide sequence and optionally a second expressible nucleotide sequence. In the method, a component or a metabolic derivative thereof when present in a low concentration in a medium is a nutrient for both the selectable genetically transformed plant cells and the non-transformed plant cells. In the method, the component or the metabolic derivative thereof when present in a high concentration in a medium is toxic to the non-transformed plant cells.
Type:
Grant
Filed:
December 23, 1999
Date of Patent:
September 3, 2002
Assignee:
Danisco A/S
Inventors:
Iain A. Donaldson, Kirsten Bojsen, Kirsten Jorgensen, Morten Jorsboe
Abstract: A method of improving the rheological properties of a flour dough and the quality of bread, alimentary paste products, noodles and cakes produced therefrom, wherein a combination of (a) a glycerol oxidase which does not require a co-factor to oxidize glycerol, and (b) a lipase, is added to the dough to produce a synergistic effect upon said rheological properties; and dough improving compositions containing such components.
Type:
Grant
Filed:
October 22, 1999
Date of Patent:
June 18, 2002
Assignee:
Danisco A/S
Inventors:
Jørn Borch Søe, Charlotte Horsmans Poulsen, Preben Rasmussen, Susan Mampusti Madrid, Masoud R. Zargahi