Patents Assigned to Danisco US Inc.
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Patent number: 11976266Abstract: The present strains and methods of the disclosure relate to genetic modifications in filamentous fungi that give rise to variant strains having altered phenotypes, altered morphologies, altered growth characteristics, and the like. More specifically, as presented and described herein, such variant strains of filamentous fungi comprising reduced viscosity phenotypes are well-suited for growth in submerged cultures (e.g., for the large-scale production of enzymes and other proteins or metabolites for commercial applications).Type: GrantFiled: April 16, 2019Date of Patent: May 7, 2024Assignee: DANISCO US INC.Inventors: Elizabeth A. Bodie, Robert James Pratt, II, Michael Ward, Jonathan M. Palmer
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Patent number: 11946080Abstract: Disclosed herein is one or more subtilisin variants, nucleic acids encoding same, and compositions and methods related to the production and uses thereof, including one or more subtilisin variants that has improved stability and/or soil removal compared to one or more reference subtilisin.Type: GrantFiled: June 18, 2017Date of Patent: April 2, 2024Assignee: DANISCO US INC.Inventors: Richard R. Bott, David A. Estell, Frits Goedegebuur, Harm Mulder, Sina Pricelius
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Patent number: 11946078Abstract: Disclosed herein are cellulase variants, or active fragments thereof, and polynucleotides encoding same, where the cellulase variants, or active fragments thereof, have endoglucanase activity. Also disclosed herein are compositions comprising the cellulase variants, or active fragments thereof; vectors and/or host cells comprising the polynucleotides encoding the cellulase variants, or active fragments thereof; and methods for making and/or using the cellulase variants, or active fragments thereof and/or compositions containing same; where the cellulase variants, or active fragments thereof, have endoglucanase activity.Type: GrantFiled: April 22, 2021Date of Patent: April 2, 2024Assignee: Danisco US Inc.Inventors: Neeraj Pandey, Sina Pricelius, Annapurna Sachan, Stepan Shipovskov, Richard Bott, Ajit Kumar Satapathy
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Patent number: 11946081Abstract: Disclosed herein is one or more subtilisin variant. nucleic acid encoding same. and compositions and methods related to the production and use thereof. including one or more Bacillus gibsonii-clade subtilisin variant that has improved soil removal compared to one or more reference subtilisin.Type: GrantFiled: December 19, 2017Date of Patent: April 2, 2024Assignee: Danisco US Inc.Inventors: Lilia Maria Babe, Lydia Dankmeyer, Frits Goedegebuur, Thijs Kaper, Harm Mulder, Sina Pricelius
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Patent number: 11920170Abstract: Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.Type: GrantFiled: December 9, 2016Date of Patent: March 5, 2024Assignee: DANISCO US INC.Inventors: William Cuevas, Vivek Sharma, David E. Wildes, Sang-Kyu Lee, Dina Finan
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Patent number: 11918011Abstract: Described are compositions and methods relate to stable, high-payload, non-porous enzyme-containing coated granules with improved resistance to activity loss during steam pelleting.Type: GrantFiled: May 30, 2019Date of Patent: March 5, 2024Assignee: DANISCO US INC.Inventors: Samuel A. Maurer, Nathaniel T. Becker, Douglas A. Dale, Peyman Moslemy, Michael Reichman, Alan Martin Allgeier
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Patent number: 11898177Abstract: Disclosed are compositions and methods relating to variant maltohexaose-forming alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.Type: GrantFiled: July 12, 2021Date of Patent: February 13, 2024Assignee: DANISCO US INC.Inventors: Luis G Cascao-Pereira, David A Estell, Marc Anton Bernhard Kolkman, Harm Mulder
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Patent number: 11879127Abstract: The instant disclosure is generally related to compositions and methods for obtaining and constructing Bacillus licheniformis host cells (e.g., protein production host cells, cell factories) having increased protein production capabilities. Certain embodiments of the disclosure are directed to efficient genetic modifications of B. licheniformis cells and the subsequent selection of such B. licheniformis cells having increased protein production capabilities. Certain other embodiments of the disclosure are generally related to methods and compositions for producing/obtaining auxotrophic B. licheniformis cells, wherein certain other embodiments of the disclosure are directed to methods and compositions for restoring prototrophy in auxotrophic B. licheniformis cells, and expressing genes of interest (GOIs) in such restored prototrophy B. licheniformis cells.Type: GrantFiled: August 21, 2018Date of Patent: January 23, 2024Assignee: DANISCO US INC.Inventors: Dennis De Lange, Marc Anton Bernhard Kolkman, Frank Wouter Koopman, Chris Leeflang
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Patent number: 11866713Abstract: The present disclosure is generally related to compositions and methods for obtaining Bacillus licheniformis cells/strains having increased protein production capabilities. Certain embodiments of the disclosure are related to genetically modified Bacillus licheniformis cells/strains derived from parental B. licheniformis cells/strains comprising a variant rghR2 gene.Type: GrantFiled: February 22, 2018Date of Patent: January 9, 2024Assignee: DANISCO US INC.Inventors: Marc Anton Bernhard Kolkman, Steven D. Doig, Ryan L. Frisch, Hongxian He, Frank Wouter Koopman, Chris Leeflang
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Patent number: 11859216Abstract: Bacillus agaradhaerens strain WDG185 expresses an inulosucrase that efficiently synthesizes a broad range of IOS with a GF range of GF3-GF30. The isolated and/or purified inulosucrase, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the inulosucrase, its variants, or its active fragments, host cells comprising the synthetic nucleic acids, and compositions comprising the inulosucrase are provided. Methods of using the compositions include the manufacture of inulooligosaccharides.Type: GrantFiled: September 17, 2020Date of Patent: January 2, 2024Assignee: Danisco US Inc.Inventors: Slavko Kralj, Marc Kolkman, Chris Leeflang, Johannes G. De Nobel, Arjen Hoekstra, Veli Alkan
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Patent number: 11802275Abstract: Reactions are disclosed herein comprising water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and an alpha-1,4-glucan phosphorylase. Novel alpha-1,4-glucan phosphorylase enzymes are also disclosed. Additional disclosures herein regard sucrose phosphorylase enzymes and methods of use thereof to produce alpha-G1P.Type: GrantFiled: July 18, 2019Date of Patent: October 31, 2023Assignee: Danisco US Inc.Inventors: Slavko Kralj, Zheyong Yu, Zhenghong Zhang
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Patent number: 11781125Abstract: Compositions and methods are provided for variant Cas systems and elements comprising such systems, including, but not limiting to, Cas endonuclease variants, guide polynucleotide/Cas endonuclease complexes comprising Cas endonuclease variants, as well as guide polynucleotides and guide RNA elements that can interact with Cas endonuclease variants. Compositions and methods are provided for genome modification of a target sequence in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system comprising a Cas9 endonuclease variant to provide an effective system for modifying or altering target sequences within the genome of a cell or organism.Type: GrantFiled: December 11, 2018Date of Patent: October 10, 2023Assignee: DANISCO US INC.Inventors: Ryan L. Frisch, Hongxian He
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Patent number: 11781147Abstract: The present disclosure is generally related to novel promoter sequences and methods thereof for enhanced protein production in Bacillus sp. (host) cells. As set forth herein, the novel promoter sequences of the disclosure, when operably linked to a gene or open reading frame encoding a protein of interest, are particularly well suited for use in large scale production of industrially relevant proteins.Type: GrantFiled: November 25, 2019Date of Patent: October 10, 2023Assignee: Danisco US Inc.Inventors: Stacey Irene Robida Stubbs, Cristina Bongiorni, Ryan L. Frisch, Chris Leeflang
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Patent number: 11685937Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, isoprenoids, and/or acetyl-CoA-derived products in recombinant microorganisms by engineering the microorganisms to comprise one or more acetylating proteins such that the expression and/or activity of the one or more acetylating proteins is modulated.Type: GrantFiled: May 11, 2020Date of Patent: June 27, 2023Assignee: Danisco US Inc.Inventors: Marguerite A. Cervin, Michael C. Miller, Derek H. Wells, Alan J. Wolfe, Madhukar S. Dasika
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Patent number: 11661567Abstract: Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.Type: GrantFiled: May 31, 2017Date of Patent: May 30, 2023Assignee: Danisco US Inc.Inventors: David Marquez, Michael Stoner, James T. Kellis, Jr., Adam Garske, Joshua Roy Basler, Katherine Augustyn, Priyanka Chandrasekaran, Miles Christopher Scotcher, Lilia Maria Babe, Richard R. Bott, David A. Estell, Gudrun Vogtentanz, Sina Pricelius, Jian Yao
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Publication number: 20230049452Abstract: The present disclosure provides lipolytic enzyme variants having two or more modifications as compared to a parent lipolytic enzyme. Specifically, the present disclosure provides one or more lipolytic enzyme variants that has at least one improved performance when compared to one or more reference lipolytic enzymes. In addition, the present disclosure provides compositions comprising a lipolytic enzyme variant of the disclosure. The present disclosure also provides methods of cleaning using compositions comprising a lipolytic enzyme variant of the disclosure.Type: ApplicationFiled: January 13, 2021Publication date: February 16, 2023Applicant: DANISCO US INCInventors: Felipe BENDEZU OSEAS, Angel ELIZABETH GRAC D'OLIVIERA, David A. ESTELL, Brian PAUL LAMB, Wilmelys MENZEZ, Jahnavi CHANDRA PRASAD, Yixin REN
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Patent number: 11525151Abstract: Described are methods for saccharifying starch-containing materials using a glucoamylase, methods for producing fermentation products, and fermentation products produced by the method thereof as well as methods for increasing starch digestibility in a ruminant using at least one of the glucoamylases described herein.Type: GrantFiled: March 6, 2019Date of Patent: December 13, 2022Assignee: DANISCO US INC.Inventors: Jing Ge, Xiaogang Gu, Helong Hao, Karsten Matthias Kragh, Jinahua (Jalsen) Li, Wenting Li, Zhongmei Tang, Shukun Yu, Bo Zhong, Kun Zhong, Zhengzheng Zou
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Patent number: 11499146Abstract: Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more Bacillus gibsonii-clade subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.Type: GrantFiled: June 17, 2016Date of Patent: November 15, 2022Assignee: DANISCO US INC.Inventors: Lilia Maria Babe, Richard R. Bott, David A. Estell, Harm Mulder, Frits Goedegebuur, Jian Yao, Sina Pricelius, Miles Scotcher
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Patent number: 11473075Abstract: The present disclosure provides AprL-clade protease enzymes, including variant AprL-clade protease enzymes, nucleic acids encoding same, and compositions and methods related to the production and use thereof, including an AprL-clade variant subtilisin enzyme that has improved stability and/or soil removal compared to a parent AprL-clade subtilisin enzyme.Type: GrantFiled: December 19, 2019Date of Patent: October 18, 2022Assignee: DANISCO US INC.Inventors: David A. Estell, Frits Goedegebuur, Marc Anton Bernhard Kolkman, Rie Mejldal, Katherine Augustyn, Lilia Maria Babe, Richard R Bott, Jian Yao, Roopa Santosh Ghirnikar
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Patent number: 11453871Abstract: Described herein is at least one novel trypsin-like serine protease polypeptide and uses thereof. Further described herein are cleaning compositions containing at least one polypeptide described herein, wherein said composition can be used to clean fabrics and hard surfaces. Even further described herein is at least one cleaning composition selected from a laundry detergent, a dishwashing detergent (e.g., automatic and hand dish), and a personal care composition. Even still further, at least one polypeptide having improved soil removal and/or stability compared to at least one reference polypeptide is described herein.Type: GrantFiled: March 8, 2018Date of Patent: September 27, 2022Assignee: Danisco US Inc.Inventors: Stepan Shipovskov, Zhengzheng Zou, Shukun Yu, Xiaogang Gu