Patents Assigned to Danisco US Inc.
  • Patent number: 11781147
    Abstract: The present disclosure is generally related to novel promoter sequences and methods thereof for enhanced protein production in Bacillus sp. (host) cells. As set forth herein, the novel promoter sequences of the disclosure, when operably linked to a gene or open reading frame encoding a protein of interest, are particularly well suited for use in large scale production of industrially relevant proteins.
    Type: Grant
    Filed: November 25, 2019
    Date of Patent: October 10, 2023
    Assignee: Danisco US Inc.
    Inventors: Stacey Irene Robida Stubbs, Cristina Bongiorni, Ryan L. Frisch, Chris Leeflang
  • Patent number: 11685937
    Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, isoprenoids, and/or acetyl-CoA-derived products in recombinant microorganisms by engineering the microorganisms to comprise one or more acetylating proteins such that the expression and/or activity of the one or more acetylating proteins is modulated.
    Type: Grant
    Filed: May 11, 2020
    Date of Patent: June 27, 2023
    Assignee: Danisco US Inc.
    Inventors: Marguerite A. Cervin, Michael C. Miller, Derek H. Wells, Alan J. Wolfe, Madhukar S. Dasika
  • Patent number: 11661567
    Abstract: Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.
    Type: Grant
    Filed: May 31, 2017
    Date of Patent: May 30, 2023
    Assignee: Danisco US Inc.
    Inventors: David Marquez, Michael Stoner, James T. Kellis, Jr., Adam Garske, Joshua Roy Basler, Katherine Augustyn, Priyanka Chandrasekaran, Miles Christopher Scotcher, Lilia Maria Babe, Richard R. Bott, David A. Estell, Gudrun Vogtentanz, Sina Pricelius, Jian Yao
  • Publication number: 20230049452
    Abstract: The present disclosure provides lipolytic enzyme variants having two or more modifications as compared to a parent lipolytic enzyme. Specifically, the present disclosure provides one or more lipolytic enzyme variants that has at least one improved performance when compared to one or more reference lipolytic enzymes. In addition, the present disclosure provides compositions comprising a lipolytic enzyme variant of the disclosure. The present disclosure also provides methods of cleaning using compositions comprising a lipolytic enzyme variant of the disclosure.
    Type: Application
    Filed: January 13, 2021
    Publication date: February 16, 2023
    Applicant: DANISCO US INC
    Inventors: Felipe BENDEZU OSEAS, Angel ELIZABETH GRAC D'OLIVIERA, David A. ESTELL, Brian PAUL LAMB, Wilmelys MENZEZ, Jahnavi CHANDRA PRASAD, Yixin REN
  • Patent number: 11525151
    Abstract: Described are methods for saccharifying starch-containing materials using a glucoamylase, methods for producing fermentation products, and fermentation products produced by the method thereof as well as methods for increasing starch digestibility in a ruminant using at least one of the glucoamylases described herein.
    Type: Grant
    Filed: March 6, 2019
    Date of Patent: December 13, 2022
    Assignee: DANISCO US INC.
    Inventors: Jing Ge, Xiaogang Gu, Helong Hao, Karsten Matthias Kragh, Jinahua (Jalsen) Li, Wenting Li, Zhongmei Tang, Shukun Yu, Bo Zhong, Kun Zhong, Zhengzheng Zou
  • Patent number: 11499146
    Abstract: Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more Bacillus gibsonii-clade subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.
    Type: Grant
    Filed: June 17, 2016
    Date of Patent: November 15, 2022
    Assignee: DANISCO US INC.
    Inventors: Lilia Maria Babe, Richard R. Bott, David A. Estell, Harm Mulder, Frits Goedegebuur, Jian Yao, Sina Pricelius, Miles Scotcher
  • Patent number: 11473075
    Abstract: The present disclosure provides AprL-clade protease enzymes, including variant AprL-clade protease enzymes, nucleic acids encoding same, and compositions and methods related to the production and use thereof, including an AprL-clade variant subtilisin enzyme that has improved stability and/or soil removal compared to a parent AprL-clade subtilisin enzyme.
    Type: Grant
    Filed: December 19, 2019
    Date of Patent: October 18, 2022
    Assignee: DANISCO US INC.
    Inventors: David A. Estell, Frits Goedegebuur, Marc Anton Bernhard Kolkman, Rie Mejldal, Katherine Augustyn, Lilia Maria Babe, Richard R Bott, Jian Yao, Roopa Santosh Ghirnikar
  • Patent number: 11453871
    Abstract: Described herein is at least one novel trypsin-like serine protease polypeptide and uses thereof. Further described herein are cleaning compositions containing at least one polypeptide described herein, wherein said composition can be used to clean fabrics and hard surfaces. Even further described herein is at least one cleaning composition selected from a laundry detergent, a dishwashing detergent (e.g., automatic and hand dish), and a personal care composition. Even still further, at least one polypeptide having improved soil removal and/or stability compared to at least one reference polypeptide is described herein.
    Type: Grant
    Filed: March 8, 2018
    Date of Patent: September 27, 2022
    Assignee: Danisco US Inc.
    Inventors: Stepan Shipovskov, Zhengzheng Zou, Shukun Yu, Xiaogang Gu
  • Patent number: 11447782
    Abstract: The instant disclosure is generally related to novel engineered (hybrid) promoters. In certain embodiments, the instant disclosure is directed to one or more nucleic acid compositions comprising engineered promoters operably linked to nucleic acids encoding proteins of interest. Thus, the disclosure set forth herein described methods and compositions for the production of proteins of interest using one or more novel engineered (hybrid) promoters of the disclosure.
    Type: Grant
    Filed: March 6, 2017
    Date of Patent: September 20, 2022
    Assignee: DANISCO US INC.
    Inventors: Cristina Bongiorni, Marc Kolkman, Chris Leeflang, Virgil Arthur Rhodius, Anita Van Kimmenade
  • Patent number: 11447762
    Abstract: The present invention provides serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.
    Type: Grant
    Filed: July 10, 2018
    Date of Patent: September 20, 2022
    Assignee: Danisco US Inc.
    Inventors: Viktor Yuryevich Alekseyev, Neelam S. Amin, Luis G. Cascao-Pereira, Katherine D. Collier, David A. Estell, James T. Kellis, Jr., Ayrookaran J. Poulose, Philip F. Souter, Glenn S. Ward, Katherine Augustyn, Jian Yao
  • Patent number: 11447783
    Abstract: Described are compositions and methods relating to modified yeast that over-express polyadenylate-binding protein (PAB1). The yeast produces a deceased amount of acetate compared to parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates where acetate in an undesirable end product.
    Type: Grant
    Filed: March 4, 2019
    Date of Patent: September 20, 2022
    Assignee: DANISCO US INC.
    Inventors: Min Qi, Paula Johanna Maria Teunissen, Quinn Qun Zhu
  • Patent number: 11441139
    Abstract: Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
    Type: Grant
    Filed: August 20, 2018
    Date of Patent: September 13, 2022
    Assignee: DANISCO US INC (157111)
    Inventors: Jonathan K. Lassila, Sandra W. Ramer
  • Patent number: 11427829
    Abstract: Compositions and methods are provided for genome modification at a target site in the genome of a filamentous fungal cell. The methods and compositions are drawn to a guide polynucleotide/Cas endonuclease system for modifying or altering the target site. Aspects in which the filamentous fungal cell being modified has a defective non-homologous end joining pathway are also provided.
    Type: Grant
    Filed: December 15, 2015
    Date of Patent: August 30, 2022
    Assignee: DANISCO US INC
    Inventors: Benjamin S. Bower, Jimmy Chan, Jing Ge, Xiaogang Gu, Susan Mampusti Madrid, Danfeng Song, Mingmin Song, Michael Ward
  • Patent number: 11414643
    Abstract: The instant disclosure is generally related to novel Bacillus sp. mutants capable of producing increased amounts of industrially relevant proteins of interest. Certain embodiments of the disclosure are related to modified Bacillus sp. cells comprising an introduced polynucleotide encoding a variant GlcT protein. Other embodiments are related to methods and compositions for producing endogenous and/or heterologous proteins of interest in the modified Bacillus sp. (daughter) cells, whereas certain other embodiments are directed to nucleic acid sequences, particularly polynucleotide open reading frame (ORF) sequences, vectors thereof and DNA expression constructs thereof, encoding variant GlcT proteins of the disclosure.
    Type: Grant
    Filed: December 12, 2018
    Date of Patent: August 16, 2022
    Assignee: DANISCO US INC.
    Inventors: Shannon Del Chase, Carol Marie Fioresi, Ryan L. Frisch, Helen Olivia Masson, Anita Van Kimmenade
  • Patent number: 11401522
    Abstract: Compositions and methods are provided for genome modification at a target site in the genome of a fungal cell. The methods and compositions are drawn to a guide polynucleotide/Cas endonuclease system for promoting modification of the DNA sequence at a target site in a fungal host cell genome.
    Type: Grant
    Filed: December 16, 2015
    Date of Patent: August 2, 2022
    Assignee: DANISCO US INC
    Inventors: Benjamin S. Bower, Jimmy Chan, Jing Ge, Xiaogang Gu, Susan Mampusti Madrid, Danfeng Song, Mingmin Song, Michael Ward, Steven Sungin Kim
  • Patent number: 11384348
    Abstract: The present disclosure relates to serine proteases cloned from Bacillus gibsonii, and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.
    Type: Grant
    Filed: December 4, 2019
    Date of Patent: July 12, 2022
    Assignee: DANISCO US INC
    Inventors: Marc Kolkman, Frits Goedegebuur, Lilia Maria Babe, Rie Mejldal, Harm Mulder, Anja Hemmingsen Kellett-Smith, Richard R Bott, Miles Christopher Scotcher
  • Patent number: 11371035
    Abstract: This present invention relates to cultured recombinant cells comprising heterologous phosphoketolase (PKL) polypeptides that are capable of increased production of acetyl coenzyme A-derived metabolites, as well as methods for producing and using the same. In some embodiments, the recombinant cells further comprise one or more mevalonate (MVA) pathway polypeptides for the production of isoprenoid precursors, isoprene and isoprenoids.
    Type: Grant
    Filed: September 24, 2020
    Date of Patent: June 28, 2022
    Assignee: Danisco US Inc.
    Inventors: Zachary Q. Beck, Jeffrey W. Munos, Derek H. Wells, Jian Yao
  • Patent number: 11306319
    Abstract: The present disclosure is generally related to modified Gram positive bacterial cells producing increased amounts of one or more protein(s) of interest and modified Gram positive bacterial cells having increased genetic competency. Thus, certain embodiments of the disclosure are directed to modified Gram positive bacterial cells expressing an increased amount of a protein of interest, relative to an unmodified (parental) Gram positive bacterial cell expressing the same protein of interest, wherein the modified bacterial cell comprises at least one mutation in a rpoC gene encoding a variant RNA-polymerase (RNAP) ??-subunit polypeptide. In certain embodiments, the rpoC gene encoding the variant ??-subunit polypeptide is integrated into the chromosome of the modified cell. In other embodiments, the rpoC gene encoding the variant ??-subunit polypeptide is comprised on an extrachromosomal plasmid introduced into the modified cell.
    Type: Grant
    Filed: October 27, 2016
    Date of Patent: April 19, 2022
    Assignee: Danisco US Inc.
    Inventors: Cristina Bongiorni, Marguerite A. Cervin, George England, Chao Zhu, Frank Wouter Koopman
  • Publication number: 20220112454
    Abstract: Entomopathogenic fungal strains, compositions, and methods and compositions of producing and using the strains for reducing overall insect damage.
    Type: Application
    Filed: December 22, 2021
    Publication date: April 14, 2022
    Applicant: DANISCO US INC.
    Inventors: ANNE-SOPHIE MADELEINE ELISABETH ALDIGUIER, JACOB ANDREW LATONE, NICOLAS LEIVA, JOSHUA KLAUS MICHEL, ALEKSANDRA VIRAG
  • Patent number: 11278041
    Abstract: Processes of treating grain (e.g., corn), involving milling the grain to produce milled grain wherein the grain germ remains intact in the milled grain, and producing a mixture by mixing the milled grain with water and at least one enzyme selected from the group consisting of protease, alpha amylase, glucoamylase, cell wall degrading enzyme, and mixtures thereof, wherein the pH of the mixture is optionally adjusted to a pH of about 3.5 to about 6.5, and incubating the mixture for about 1 to about 3 hours to produce an incubated mixture.
    Type: Grant
    Filed: January 30, 2020
    Date of Patent: March 22, 2022
    Assignees: The United States of America, as represented by the Secretary of Agriculture, DANISCO US INC.
    Inventors: David Johnston, Jayarama K. Shetty, Bart C. Koops