Abstract: The present disclosure is generally related to novel promoter sequences and methods thereof for enhanced protein production in Bacillus sp. (host) cells. As set forth herein, the novel promoter sequences of the disclosure, when operably linked to a gene or open reading frame encoding a protein of interest, are particularly well suited for use in large scale production of industrially relevant proteins.
Type:
Grant
Filed:
November 25, 2019
Date of Patent:
October 10, 2023
Assignee:
Danisco US Inc.
Inventors:
Stacey Irene Robida Stubbs, Cristina Bongiorni, Ryan L. Frisch, Chris Leeflang
Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, isoprenoids, and/or acetyl-CoA-derived products in recombinant microorganisms by engineering the microorganisms to comprise one or more acetylating proteins such that the expression and/or activity of the one or more acetylating proteins is modulated.
Type:
Grant
Filed:
May 11, 2020
Date of Patent:
June 27, 2023
Assignee:
Danisco US Inc.
Inventors:
Marguerite A. Cervin, Michael C. Miller, Derek H. Wells, Alan J. Wolfe, Madhukar S. Dasika
Abstract: Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.
Type:
Grant
Filed:
May 31, 2017
Date of Patent:
May 30, 2023
Assignee:
Danisco US Inc.
Inventors:
David Marquez, Michael Stoner, James T. Kellis, Jr., Adam Garske, Joshua Roy Basler, Katherine Augustyn, Priyanka Chandrasekaran, Miles Christopher Scotcher, Lilia Maria Babe, Richard R. Bott, David A. Estell, Gudrun Vogtentanz, Sina Pricelius, Jian Yao
Abstract: The present disclosure provides lipolytic enzyme variants having two or more modifications as compared to a parent lipolytic enzyme. Specifically, the present disclosure provides one or more lipolytic enzyme variants that has at least one improved performance when compared to one or more reference lipolytic enzymes. In addition, the present disclosure provides compositions comprising a lipolytic enzyme variant of the disclosure. The present disclosure also provides methods of cleaning using compositions comprising a lipolytic enzyme variant of the disclosure.
Type:
Application
Filed:
January 13, 2021
Publication date:
February 16, 2023
Applicant:
DANISCO US INC
Inventors:
Felipe BENDEZU OSEAS, Angel ELIZABETH GRAC D'OLIVIERA, David A. ESTELL, Brian PAUL LAMB, Wilmelys MENZEZ, Jahnavi CHANDRA PRASAD, Yixin REN
Abstract: Described are methods for saccharifying starch-containing materials using a glucoamylase, methods for producing fermentation products, and fermentation products produced by the method thereof as well as methods for increasing starch digestibility in a ruminant using at least one of the glucoamylases described herein.
Type:
Grant
Filed:
March 6, 2019
Date of Patent:
December 13, 2022
Assignee:
DANISCO US INC.
Inventors:
Jing Ge, Xiaogang Gu, Helong Hao, Karsten Matthias Kragh, Jinahua (Jalsen) Li, Wenting Li, Zhongmei Tang, Shukun Yu, Bo Zhong, Kun Zhong, Zhengzheng Zou
Abstract: Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more Bacillus gibsonii-clade subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.
Type:
Grant
Filed:
June 17, 2016
Date of Patent:
November 15, 2022
Assignee:
DANISCO US INC.
Inventors:
Lilia Maria Babe, Richard R. Bott, David A. Estell, Harm Mulder, Frits Goedegebuur, Jian Yao, Sina Pricelius, Miles Scotcher
Abstract: The present disclosure provides AprL-clade protease enzymes, including variant AprL-clade protease enzymes, nucleic acids encoding same, and compositions and methods related to the production and use thereof, including an AprL-clade variant subtilisin enzyme that has improved stability and/or soil removal compared to a parent AprL-clade subtilisin enzyme.
Type:
Grant
Filed:
December 19, 2019
Date of Patent:
October 18, 2022
Assignee:
DANISCO US INC.
Inventors:
David A. Estell, Frits Goedegebuur, Marc Anton Bernhard Kolkman, Rie Mejldal, Katherine Augustyn, Lilia Maria Babe, Richard R Bott, Jian Yao, Roopa Santosh Ghirnikar
Abstract: Described herein is at least one novel trypsin-like serine protease polypeptide and uses thereof. Further described herein are cleaning compositions containing at least one polypeptide described herein, wherein said composition can be used to clean fabrics and hard surfaces. Even further described herein is at least one cleaning composition selected from a laundry detergent, a dishwashing detergent (e.g., automatic and hand dish), and a personal care composition. Even still further, at least one polypeptide having improved soil removal and/or stability compared to at least one reference polypeptide is described herein.
Abstract: The instant disclosure is generally related to novel engineered (hybrid) promoters. In certain embodiments, the instant disclosure is directed to one or more nucleic acid compositions comprising engineered promoters operably linked to nucleic acids encoding proteins of interest. Thus, the disclosure set forth herein described methods and compositions for the production of proteins of interest using one or more novel engineered (hybrid) promoters of the disclosure.
Type:
Grant
Filed:
March 6, 2017
Date of Patent:
September 20, 2022
Assignee:
DANISCO US INC.
Inventors:
Cristina Bongiorni, Marc Kolkman, Chris Leeflang, Virgil Arthur Rhodius, Anita Van Kimmenade
Abstract: The present invention provides serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.
Type:
Grant
Filed:
July 10, 2018
Date of Patent:
September 20, 2022
Assignee:
Danisco US Inc.
Inventors:
Viktor Yuryevich Alekseyev, Neelam S. Amin, Luis G. Cascao-Pereira, Katherine D. Collier, David A. Estell, James T. Kellis, Jr., Ayrookaran J. Poulose, Philip F. Souter, Glenn S. Ward, Katherine Augustyn, Jian Yao
Abstract: Described are compositions and methods relating to modified yeast that over-express polyadenylate-binding protein (PAB1). The yeast produces a deceased amount of acetate compared to parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates where acetate in an undesirable end product.
Type:
Grant
Filed:
March 4, 2019
Date of Patent:
September 20, 2022
Assignee:
DANISCO US INC.
Inventors:
Min Qi, Paula Johanna Maria Teunissen, Quinn Qun Zhu
Abstract: Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
Abstract: Compositions and methods are provided for genome modification at a target site in the genome of a filamentous fungal cell. The methods and compositions are drawn to a guide polynucleotide/Cas endonuclease system for modifying or altering the target site. Aspects in which the filamentous fungal cell being modified has a defective non-homologous end joining pathway are also provided.
Type:
Grant
Filed:
December 15, 2015
Date of Patent:
August 30, 2022
Assignee:
DANISCO US INC
Inventors:
Benjamin S. Bower, Jimmy Chan, Jing Ge, Xiaogang Gu, Susan Mampusti Madrid, Danfeng Song, Mingmin Song, Michael Ward
Abstract: The instant disclosure is generally related to novel Bacillus sp. mutants capable of producing increased amounts of industrially relevant proteins of interest. Certain embodiments of the disclosure are related to modified Bacillus sp. cells comprising an introduced polynucleotide encoding a variant GlcT protein. Other embodiments are related to methods and compositions for producing endogenous and/or heterologous proteins of interest in the modified Bacillus sp. (daughter) cells, whereas certain other embodiments are directed to nucleic acid sequences, particularly polynucleotide open reading frame (ORF) sequences, vectors thereof and DNA expression constructs thereof, encoding variant GlcT proteins of the disclosure.
Type:
Grant
Filed:
December 12, 2018
Date of Patent:
August 16, 2022
Assignee:
DANISCO US INC.
Inventors:
Shannon Del Chase, Carol Marie Fioresi, Ryan L. Frisch, Helen Olivia Masson, Anita Van Kimmenade
Abstract: Compositions and methods are provided for genome modification at a target site in the genome of a fungal cell. The methods and compositions are drawn to a guide polynucleotide/Cas endonuclease system for promoting modification of the DNA sequence at a target site in a fungal host cell genome.
Type:
Grant
Filed:
December 16, 2015
Date of Patent:
August 2, 2022
Assignee:
DANISCO US INC
Inventors:
Benjamin S. Bower, Jimmy Chan, Jing Ge, Xiaogang Gu, Susan Mampusti Madrid, Danfeng Song, Mingmin Song, Michael Ward, Steven Sungin Kim
Abstract: The present disclosure relates to serine proteases cloned from Bacillus gibsonii, and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.
Type:
Grant
Filed:
December 4, 2019
Date of Patent:
July 12, 2022
Assignee:
DANISCO US INC
Inventors:
Marc Kolkman, Frits Goedegebuur, Lilia Maria Babe, Rie Mejldal, Harm Mulder, Anja Hemmingsen Kellett-Smith, Richard R Bott, Miles Christopher Scotcher
Abstract: This present invention relates to cultured recombinant cells comprising heterologous phosphoketolase (PKL) polypeptides that are capable of increased production of acetyl coenzyme A-derived metabolites, as well as methods for producing and using the same. In some embodiments, the recombinant cells further comprise one or more mevalonate (MVA) pathway polypeptides for the production of isoprenoid precursors, isoprene and isoprenoids.
Type:
Grant
Filed:
September 24, 2020
Date of Patent:
June 28, 2022
Assignee:
Danisco US Inc.
Inventors:
Zachary Q. Beck, Jeffrey W. Munos, Derek H. Wells, Jian Yao
Abstract: The present disclosure is generally related to modified Gram positive bacterial cells producing increased amounts of one or more protein(s) of interest and modified Gram positive bacterial cells having increased genetic competency. Thus, certain embodiments of the disclosure are directed to modified Gram positive bacterial cells expressing an increased amount of a protein of interest, relative to an unmodified (parental) Gram positive bacterial cell expressing the same protein of interest, wherein the modified bacterial cell comprises at least one mutation in a rpoC gene encoding a variant RNA-polymerase (RNAP) ??-subunit polypeptide. In certain embodiments, the rpoC gene encoding the variant ??-subunit polypeptide is integrated into the chromosome of the modified cell. In other embodiments, the rpoC gene encoding the variant ??-subunit polypeptide is comprised on an extrachromosomal plasmid introduced into the modified cell.
Type:
Grant
Filed:
October 27, 2016
Date of Patent:
April 19, 2022
Assignee:
Danisco US Inc.
Inventors:
Cristina Bongiorni, Marguerite A. Cervin, George England, Chao Zhu, Frank Wouter Koopman
Abstract: Entomopathogenic fungal strains, compositions, and methods and compositions of producing and using the strains for reducing overall insect damage.
Type:
Application
Filed:
December 22, 2021
Publication date:
April 14, 2022
Applicant:
DANISCO US INC.
Inventors:
ANNE-SOPHIE MADELEINE ELISABETH ALDIGUIER, JACOB ANDREW LATONE, NICOLAS LEIVA, JOSHUA KLAUS MICHEL, ALEKSANDRA VIRAG
Abstract: Processes of treating grain (e.g., corn), involving milling the grain to produce milled grain wherein the grain germ remains intact in the milled grain, and producing a mixture by mixing the milled grain with water and at least one enzyme selected from the group consisting of protease, alpha amylase, glucoamylase, cell wall degrading enzyme, and mixtures thereof, wherein the pH of the mixture is optionally adjusted to a pH of about 3.5 to about 6.5, and incubating the mixture for about 1 to about 3 hours to produce an incubated mixture.
Type:
Grant
Filed:
January 30, 2020
Date of Patent:
March 22, 2022
Assignees:
The United States of America, as represented by the Secretary of Agriculture, DANISCO US INC.
Inventors:
David Johnston, Jayarama K. Shetty, Bart C. Koops