Patents Assigned to DH Technologies Development Pte. Ltd.
  • Patent number: 12224171
    Abstract: An electrospray probe for use in an electrospray ion source is disclosed, which comprises a cannula extending from a proximal end having an inlet aperture for receiving a liquid sample containing at least one analyte to a discharge emitter end having an outlet aperture through which charged liquid droplets containing ions of said analyte are discharged, and an electrically conductive coating covering at least a portion of an external surface and at least a portion of an internal surface of said emitter end.
    Type: Grant
    Filed: May 29, 2020
    Date of Patent: February 11, 2025
    Assignee: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
    Inventors: Leigh Bedford, Goran Ristic, Bradley B. Schneider
  • Patent number: 12209989
    Abstract: MS-based methods and systems are provided herein in which a desorption solvent desorbs one or more analyte species from an SPME device within a sampling interface that is fluidly coupled to an ion source for subsequent mass spectrometric analysis. In accordance with various aspects of the applicants teachings, the sampling interface includes an internal sampling conduit that provides increased interaction between the desorption solvent and the sampling substrate, thereby improving mass transfer (e.g., increased extraction or desorption speed).
    Type: Grant
    Filed: September 29, 2020
    Date of Patent: January 28, 2025
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: Thomas R. Covey, Chang Liu
  • Publication number: 20250022700
    Abstract: Methods and systems for mass spectrometry are disclosed. In one example, a method comprises: receiving, by a mass spectrometer via a sampling system operably connected thereto, at least one sample containing at least one known compound; modulat-ing at least one instrument parameter of the mass spectrometer through a plurality of instrument parameter values; analyzing the at least one sample while applying each of the plurality of instrument parameter values; acquiring a plurality of mass spectral (MS) datasets each corresponding to one of the applied plurality of instrument parameter values; encoding each of the plurality of MS datasets to generate a corresponding plurality of MS results each corresponding to one of the applied instrument parameter values; and compiling and storing the MS datasets and MS results in a spectral library in association with the applied instrument parameter values.
    Type: Application
    Filed: November 30, 2022
    Publication date: January 16, 2025
    Applicant: DH Technologies Development Pte. Ltd.
    Inventors: Stephen A. TATE, Christopher LOCK
  • Patent number: 12196709
    Abstract: A DMS device receives a curtain gas that includes a chemical modifier into its curtain plate. Before or while receiving ions of an analyte, the DMS device steps the CoV through a series of values in order to apply different CoV values to at least one precursor ion derived from the chemical modifier. For each CoV value of the series of values, a mass spectrometer selects and mass analyzes the at least one precursor ion. An intensity is produced for each CoV value of the series of values. An intensity versus CoV value peak is calculated from the intensities measured. A representative CoV value is calculated for the peak. The difference between the representative CoV value and known CoV values that represent an uncontaminated curtain plate is calculated. If the difference is greater than or equal to a predetermined threshold value, the curtain plate is determined to be contaminated.
    Type: Grant
    Filed: January 14, 2021
    Date of Patent: January 14, 2025
    Assignee: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
    Inventors: John Lawrence Campbell, Brendon Seale
  • Patent number: 12188944
    Abstract: Method and devices for performing top down analysis of an antibody are described which involve utilizing an ion source to generate a plurality of ions from a sample containing at least one intact antibody. Further, transmitting said plurality of ions through a quadrupole rod set while applying an RF signal thereto and in the absence of a resolving DC voltage so as to preferentially transmit precursor ions having an m/z value greater than a low mass cutoff of about 1500 m/z from the quadrupole rod set to an ECD cell. The method and device can also performing an ECD reaction in the ECD cell on said precursor ions and also detect reaction products from the ECD reaction.
    Type: Grant
    Filed: May 13, 2020
    Date of Patent: January 7, 2025
    Assignee: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
    Inventors: Takashi Baba, Pavel Ryumin
  • Patent number: 12191132
    Abstract: Before a sample is introduced into a liquid sample delivery device, an ion source device receives aqueous mobile phase solution from the liquid sample delivery device and ionizes compounds of the aqueous mobile phase solution, producing an ion beam. A tandem mass spectrometer performs a first neutral loss scan of the ion beam with a first neutral loss value set to a molecular weight of a first known solvent, producing a first intensity, and performs a second neutral loss scan of the ion beam with a second neutral loss value set to a molecular weight of a second known solvent, producing a second intensity. A ratio of the first intensity to the second intensity is calculated. It is determined if the aqueous mobile phase solution is properly being delivered by the liquid sample delivery device based on the ratio.
    Type: Grant
    Filed: August 22, 2020
    Date of Patent: January 7, 2025
    Assignee: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
    Inventors: Yves Le Blanc, David M. Cox
  • Patent number: 12191131
    Abstract: A high-voltage power supply system for a mass spectrometer comprises a ground-referenced power supply with a first transformer having a primary winding and a secondary winding, the primary winding is electrically coupled to a first source of AC power, and a floated bias voltage power supply with a second transformer having a primary winding and a secondary winding, the primary winding of the second transformer is electrically coupled to a second source of AC power. A return electrical path of the floated bias voltage power supply is electrically coupled to the ground-referenced power supply to bias an output voltage of the ground-referenced power supply. A floating shield is around the floating bias voltage power supply, and at least one resistive element is in the return electrical path of the floated bias voltage power supply to reduce noise coupled from the floated bias voltage power supply to the ground-referenced power supply.
    Type: Grant
    Filed: September 24, 2020
    Date of Patent: January 7, 2025
    Assignee: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
    Inventors: Manuel Faur, Ernesto Gradin
  • Publication number: 20240412962
    Abstract: Methods and systems for adjusting instrument setting, improving fidelity of isotope pattern for mass spectra, and/or determining molecular mass with improved accuracy are disclosed. In one example, a method for determining Mmono of a compound of interest in a sample using a mass spectrometer is provided. The method comprises: (1) tuning or adjusting instrument setting of the mass spectrometer using at least one known compound, wherein the instrument setting comprises at least one parameter for improving accuracy; (2) analyzing the compound of interest using the adjusted instrument setting to obtain a mass spectrum thereof, wherein the mass spectrum comprises an isotope pattern thereof; and determining the Mmono of the compound of interest from the mass spectrum thereof.
    Type: Application
    Filed: September 9, 2022
    Publication date: December 12, 2024
    Applicant: DH Technologies Development Pte. Ltd.
    Inventors: Eva DUCHOSLAV, Yves LE BLANC
  • Publication number: 20240404807
    Abstract: A method for determining a convolved peak intensity in a sample trace includes ejecting a plurality of sample ejections from a sample well plate. An ejection time log is generated which includes an ejection time of each of the plurality of sample ejections from the sample well plate. The plurality of sample ejections is analyzed with a mass analyzer. The sample trace of intensity versus time values is produced for the plurality of sample ejections based on the analysis. A known peak shape is obtained. A convolved peak intensity is determined for a convolved peak of the sample trace based at least in part on the known peak shape and the ejection time log.
    Type: Application
    Filed: September 23, 2022
    Publication date: December 5, 2024
    Applicant: DH Technologies Development Pte. Ltd.
    Inventors: Gordana IVOSEV, Chang LIU
  • Publication number: 20240404646
    Abstract: Methods and system that determines disulfide and trisulfide linkages within analytes (e.g., polypeptides) is described. In certain aspects. a sample comprising polypeptides (such as an antibody) may be subjected to dissociation using an electron activated dissociation (which can include electron capture dissociation and electron transfer dissociation) and the fragmentated portions are analyzed using a mass spectrometer to produce a spectrum. The spectrum is analyzed by a processor to identify peaks from the spectrum that are related to one another in the spectra by a separation of 32 mass units. In identifying an antibody comprising peptide segments linked via a trisulfide bond. for example, four different peaks representing two different peptides are searched for and identified representing a first peptide portion having mass/charge of A and A+32 and a second peptide having mass/charge of B and B+32.
    Type: Application
    Filed: September 21, 2022
    Publication date: December 5, 2024
    Applicant: DH Technologies Development Pte. Ltd.
    Inventor: Yuzhuo ZHANG
  • Publication number: 20240395527
    Abstract: A method of ejecting a sample from a nebulizer nozzle fluidically coupled to a port via a transfer conduit includes receiving at the port a transport liquid and the sample. The transport liquid and the sample in the transfer conduit is transported from the port to a transfer conduit exit comprising an electrode tip. The transport liquid is ejected from the transfer conduit exit. The sample is ejected from the transfer conduit exit substantially simultaneously with ejecting the transport liquid. During ejection of the transport liquid and the sample from the transfer conduit exit, a pressure is generated at the transfer conduit exit substantially similar to a vapor pressure of the transport liquid.
    Type: Application
    Filed: September 9, 2022
    Publication date: November 28, 2024
    Applicant: DH Technologies Development Pte. Ltd.
    Inventor: Peter KOVARIK
  • Patent number: 12154776
    Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g.
    Type: Grant
    Filed: March 14, 2023
    Date of Patent: November 26, 2024
    Assignees: Labcyte Inc., DH Technologies Development Pte. Ltd.
    Inventors: Sammy Datwani, Don W. Arnold, Lucien P. Ghislain, Chang Liu, Thomas Covey
  • Publication number: 20240377369
    Abstract: Methods and systems for identifying analytes in a sample using mass spectrometry are provided. A method for identifying analytes in mass spectrometry data comprises: introducing a sample to a mass spectrometer; analyzing the sample with the mass spectrometer in a plurality of cycles; generating, for each cycle, a mass spectrum comprising at least one peak; annotating peaks in the mass spectrum based on their relationships; assigning best ion types to each peak; processing each cycle of the mass spectrum to assign a score to each of the at least one peak thereof with respect to the likely neutral mass related to the peak; grouping peaks that share a common neutral mass; and outputting the analyte neutral mass.
    Type: Application
    Filed: September 9, 2022
    Publication date: November 14, 2024
    Applicant: DH Technologies Development Pte. Ltd.
    Inventors: Eva DUCHOSLAV, Pradeep NARAYANASWAMY, Stephen TATE
  • Patent number: 12138606
    Abstract: A fluid processing system that can include a sample container having a sample chamber for containing a fluid and a plurality of magnetic particles and at least one movable magnetic assembly configured to be movably inserted into or out of the sample chamber. The movable magnetic assembly can include a plurality of electromagnets that generate a magnetic field within at least a portion of the sample chamber when the assembly is inserted at least partially into the sample chamber. The fluid processing system can also include a signal generator that applies electrical signals, e.g., AC electrical signals, to the electromagnets of the magnetic assembly and a controller coupled to the signal generator that is configured to control phases of the electrical signals applied to the electromagnets to generate magnetic field gradients within the portion of the sample chamber effective to magnetically influence the plurality of the magnetic particles.
    Type: Grant
    Filed: November 20, 2018
    Date of Patent: November 12, 2024
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: Don W Arnold, Thomas R. Covey, Chang Liu
  • Publication number: 20240371474
    Abstract: Methods and systems for building and using an analyte library are provided. One aspect is a method for building an analyte library, the method comprising receiving mass spectrum data from analysis of a sample using mass spectrometry, mass spectrum data including a mass spectrum and a sample matrix, and the sample including an analyte, identifying peaks in the mass spectrum, assigning at least one ion type to the peaks, annotating the peaks for the analyte based on the sample matrix, extracting an ion fingerprint for the analyte based on the annotated peaks and storing an analyte identification entry including the ion fingerprint for the analyte.
    Type: Application
    Filed: September 8, 2022
    Publication date: November 7, 2024
    Applicant: DH Technologies Development Pte. Ltd.
    Inventors: Eva DUCHOSLAV, Pradeep NARAYANASWAMY, Stephen TATE
  • Patent number: 12135969
    Abstract: Electromagnetic systems and corresponding methods for assembling the electromagnetic systems are described. The electromagnetic systems can be used in fluid processing systems that include a plurality of fluid containers, each configured to define a fluid chamber that receives a fluid and a plurality of magnetic particles, and a plurality of electromagnets configured to generate a magnetic field within at least one of the plurality of the fluid containers. The fluid processing system can also include a PCB board that supplies the electromagnets with electrical current by establishing an electrical connection between electrical contact terminals included on the PCB board and spring loaded connections included on each electromagnet.
    Type: Grant
    Filed: July 19, 2019
    Date of Patent: November 5, 2024
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: Don W. Arnold, Thomas R. Covey, Chang Liu, Stanislaw Potyrala, Alex Tsipirovich
  • Publication number: 20240355605
    Abstract: A method for improved mass spectrometry by determining charge state of precursor ions from an analysis of product ions, includes receiving sample ions. A group of precursor ions is selected from the received sample ions based on mobility. A fragmentation device fragments the group of precursor ions to produce a group of product ions. A tandem mass spectrometry analysis is performed on the group of product ions to generate an intensity and mass-to-charge ratio (m/z) of the group of product ions. An ionogram is generated, based on the generated intensities and mass, to charge ratios for the groups of product ions generated for each of the mobility selection. The ionogram includes a first axis representing compensation voltage value and another axis representing intensity. A product ion peak is identified in the ionogram. At least one peak characteristic is identified of the product ion peak.
    Type: Application
    Filed: September 2, 2022
    Publication date: October 24, 2024
    Applicant: DH Technologies Development Pte. Ltd.
    Inventors: Yves LE BLANC, Gordana IVOSEV
  • Publication number: 20240355604
    Abstract: Systems and methods are disclosed for analyzing a sample using overlapping precursor isolation windows. A mass analyzer of a tandem mass spectrometer is instructed to select and fragment at least two overlapping precursor isolation windows across a precursor ion mass range of a sample using a processor. The tandem mass spectrometer includes a mass analyzer that allows overlapping precursor isolation windows across the mass range of the sample.
    Type: Application
    Filed: July 1, 2024
    Publication date: October 24, 2024
    Applicants: DH Technologies Development Pte. Ltd., ETH Zurich
    Inventors: Ronald F. Bonner, Stephen A. Tate, Rudolf Aebersold, Pedro Jose Navarro Alvarez, Oliver Rinner, Lukas Reiter, Ludovic Gillet
  • Publication number: 20240347331
    Abstract: Methods for correlating a product ion in a mass spectrum to a precursor ion are disclosed herein, comprising determining a precursor ion ml z corresponding to the product ion as an m/z at which the product ion appears in a maximum amount of the series of mass spectra. Methods also can comprise obtaining a series of mass spectra for a sample across a mass range, each of the series of mass spectra having a precursor ion transmission window defined by a width (W) that overlaps with that of at least two of the series of mass spectra by a step size (S).
    Type: Application
    Filed: August 10, 2022
    Publication date: October 17, 2024
    Applicant: DH Technologies Development Pte. Ltd.
    Inventors: Gordana IVOSEV, Nic BLOOMFIELD
  • Publication number: 20240347141
    Abstract: Methods and systems for identifying one or more analytes in a sample are provided. One aspect is a method of predicting an identity of analytes in an unknown sample, the method comprising accessing a database comprising a plurality of results from analyzing samples using mass spectrometry to identify analytes, the plurality of results including annotated ion fingerprints, training a machine learning model with the plurality of results, and applying the machine learning model to the unknown sample to predict an identity of one or more analytes in the unknown sample.
    Type: Application
    Filed: September 8, 2022
    Publication date: October 17, 2024
    Applicant: DH Technologies Development Pte. Ltd.
    Inventors: Eva DUCHOSLAV, Pradeep NARAYANASWAMY, Stephen TATE