Abstract: A computerized method and system for electronic management of input content provides for summary document generation. The method and system determines section lines for each of multiple content sections. Therein, field lines are determined for each field within the content section, including dividing up the number of section lines amongst the plurality of fields. The method and system updates an electronic user interface indicating fields and number of lines for each of fields. Via the user interface, the method and system includes receiving user input for each of the plurality of fields and tracking the user input for each of the plurality of fields relative to the number of field lines for each of the plurality of fields. Thereby, the method and system manages summary documentation generation by interacting with the user to modify the user input and available field lines, as well as updating corresponding content sections.

Abstract: Provided is an apparatus for disinfecting an object by at least partially removing a biologically-active contaminant there from. A housing encloses a disinfection chamber in which a portion of the object is to be received to be disinfected, and includes an inlet aperture through which the object is introduced to the apparatus. An ultraviolet light source emits ultraviolet light to be imparted on the portion of the object introduced to the disinfection chamber for deactivating at least a portion of the biologically-active contaminant present on the object. A controller controls operation of at least one of a feeder and the ultraviolet light to achieve a level of disinfection of the object, rendering the object suitable for use in a substantially-sterile application.

Abstract: Degenerate primers which hybridize with various classes of antibiotic biosynthesis genes were used to amplify fragments of DNA from soil and lichen extracts. Cloning and sequencing of the amplified products showed that these products included a variety of novel and previously uncharacterized antibiotic biosynthesis gene sequences, the products of which have the potential to be active as antibiotics, immunosuppressors, antitumor agents, etc.

Abstract: Xylanase DNA is recovered from soil by PCR amplification using degenerate primers. Because of the complexity of the soil samples, it is likely that the recovered product will include more than one species of polynucleotide. These recovered copies may be cloned into a host organism to produce additional copies of each individual species prior to characterization by sequencing. Recovered DNA which is found to vary from known xylanases can be used in several ways to facilitate production of novel xylanases for industrial application. First, the recovered DNA, or probes corresponding to portions thereof, can be used as a probe to screen DNA libraries and recover intact xylanase genes including the unique regions of the recovered DNA. Second, the recovered DNA or polynucleotides corresponding to portions thereof, can be inserted into a known xylanase gene to produce a recombinant xylanase gene with the sequence variations of the recovered DNA.

Abstract: A number of bacterial cultures were screened for the ability to inhibit sporulation using a strain Streptomyces 85E. Evaluations of the cultures which tested positive in this assay led to the discovery that lipopeptide biosurfactants such as surfactin and viscosin are effective inhibitors of eukaryotic protein kinase activity. Thus, a method for inhibiting eukaryotic protein kinase activity present in a sample or organism includes the step of adding to the sample or organism an effective inhibitory amount of a lipopeptide biosurfactant.

Abstract: Degenerate primers which hybridize with various classes of antibiotic biosynthesis genes were used to amplify fragments of DNA from soil and lichen extracts. Cloning and sequencing of the amplified products showed that these products included a variety of novel and previously uncharacterized antibiotic biosynthesis gene sequences, the products of which have the potential to be active as antibiotics, immunosuppressors, antitumor agents, etc.

Abstract: Xylanase DNA is recovered from soil by PCR amplification using degenerate primers. Because of the complexity of the soil samples, it is likely that the recovered product will include more than one species of polynucleotide. These recovered copies may be cloned into a host organism to produce additional copies of each individual species prior to characterization by sequencing. Recovered DNA which is found to vary from known xylanases can be used in several ways to facilitate production of novel xylanases for industrial application. First, the recovered DNA, or probes corresponding to portions thereof, can be used as a probe to screen DNA libraries and recover intact xylanase genes including the unique regions of the recovered DNA. Second, the recovered DNA or polynucleotides corresponding to portions thereof, can be inserted into a known xylanase gene to produce a recombinant xylanase gene with the sequence variations of the recovered DNA.

Abstract: Materials can be assayed for activity as an inhibitor of post-translational protein phosphorylation by adding the material to a growing culture of a prokaryotic organism such as a streptomycete; allowing the culture to grow for a period of time in the presence of the material; and observing the culture for altered development relative to development of the prokaryotic organism grown in the absence of the material. Observation of altered development is indicative that the material has activity as an inhibitor of post-translational protein phosphorylation. In particular, the material to be tested can be added to a growing culture of the prokaryotic organism by placing a carrier disk bearing the material on a freshly seeded plate. Inhibition of the development of aerial mycelia and spore formation is an indicator that the material has activity as an inhibitor of post-translational protein phosphorylation.

Abstract: An insert for an inflatable, buoyant toroid has a bottom portion with one or more contoured, concave surfaces radially disposed about a circular base and one or more seating portions above the bottom portion. The center of gravity of the insert is on a vertical axis passing substantially through the center of the circular base whether the insert contains a passenger or not. A flotation device comprising the insert and an inflatable, buoyant toroid has positive stability in water. The center of gravity of the insert is adjusted by a ballasting apparatus. The insert is provided with a variety of comfort enhancing options. The insert includes fastening devices for fastening the insert to an inflatable buoyant toroid. An insert can be provided with a top portion including an access door for using the insert as an equipment storage device.