Abstract: The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT).

Abstract: 3-substituted dihydronepetalactone is prepared by deprotonation of nepetalactone and treatment with Grignard reagent to form alkyl and aryl substituted compounds. The compounds so prepared are odorous and have a wide range of utility ranging from insect repellents to fragrance compounds, such as perfumes, among others.

Abstract: A novel tyrosine ammonia lyase enzyme was identified in the bacterium Rhodobacter sphaeroides. This enzyme has a higher activity for tyrosine than for phenylalanine and is useful for the production of para-hydroxycinnamic acid directly from tyrosine. The gene encoding this enzyme was cloned by direct amplification using the genomic DNA and was expressed in E. coli.

Abstract: This invention relates to a process which produces flat, distortion-free, zero-shrink, low-temperature co-fired ceramic (LTCC) bodies, composites, modules or packages from precursor green (unfired) laminates of three or more different dielectric tape chemistries that are configured in an uniquely or pseudo-symmetrical arrangement in the z-axis of the laminate.

Abstract: Use of water-insoluble organic nitrites and/or nitrates as additives for electrodeposition coating compositions, and process for the production of an electrodeposition lacquer with improved adhesion for subsequent coats in which one or more water-insoluble organic nitrites and/or nitrates is added to the electrodeposition coating composition and the coating film obtained by electrodeposition is stoved in an indirectly heated circulating air oven.

Abstract: In an air-velocity sensor and a method for operating an air-velocity sensor, the air-velocity sensor includes a temperature sensor arranged in an air flow and sequential electronics. The sequential electronics are used to cyclically impinge upon the temperature sensor with a define heat output and to determine the temperature rise time required by the temperature sensor to heat up to a given temperature difference after being impinged upon with the heat output. Temperature measurements are performed at a defined measuring frequency. The velocity of the air flow is determined on the basis of temperature rise time.

Abstract: This invention relates to a process which produces flat, distortion-free, zero-shrink, low-temperature co-fired ceramic (LTCC) bodies, composites, modules or packages from precursor green (unfired) laminates of three or more different dielectric tape chemistries that are configured in an uniquely or pseudo-symmetrical arrangement in the z-axis of the laminate.

Abstract: A method and system for the insertion of local signals, including digital media advertisements, into statistically multiplexed streams is presented. The rate control and timing information is computed and is used to specify the insertion time and rate parameters for digital advertisements. In one embodiment, a maximum bit rate over the advertisement duration is specified. The maximum bit rate may be constant or may vary in time, such that high bit rate portions of the advertisement are supported. High bit rate portions of the advertisements in different program streams may be staggered, such that the total bandwidth required does not exceed a maximum, but allowing for high bit rate portions of advertisements. Custom bit rate profiles for advertisements may also be defined, with the profiles being defined at a high granularity or a low granularity.

Abstract: A repeat prescription ordering system for allowing patients requiring resupply of medication or medical products to access a server using a portable communications and data processing device such as a smart phone or personal digital assistant. The server supplies to the patient a list of medication and medical products which they are authorized to order. The patient can select the products required, and the order is logged by the server and allocated to a supplier for completion of the order. The server maintains an estimate of the amount of medication or medical product held by the patient, this being based on the prescribed dosage regimen and information entered by the patient on their usage and, optionally, on checks on their own health. The patient may be alerted when the estimate indicates that their supplies are running low. The estimate is allowed to go below zero, this implying a possible departure from the prescribed medication regimen.

Abstract: Genes have been isolated from Rhodococcus and Deinococcus which encode a specific lycopene ?-cyclase capable of converting acyclic carotenoids with at least one ?-end group to the corresponding asymmetric carotenoid containing a single ?-ionone ring end group. The genes are new. Transformed host cells expressing the present genes and methods for the bio-conversion of acylic carotenoid substrates to corresponding asymmetric carotenoid are also provided.

Abstract: The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, and seed thereof, transformed with the nucleic acid molecules of the invention so as to confer disease resistance on the plant.

Abstract: This invention relates to the generation of animals by the transfer of somatic cells which have been denatured preferably by heating, into enucleated metaphase II oocytes. This invention may be useful for the cloning of mammals including but not being limited to genetically selected and/or transgenic animals.

Abstract: The invention provides in one aspect a process for preparing a microgel by RAFT polymerizing in the presence of a RAFT chain transfer agent, one or more solvophobic monomers and one or more solvophilic monomers to form one or more block copolymers comprising one or more solvophobic blocks and one or more solvophilic blocks wherein the solvophobic block is insoluble in a dispersing medium and the solvophilic block is soluble in the dispersing medium. The block copolymer is dispersed in the dispersing medium to form micelles, which is then stabilized to form the microgel. The dispersing medium can be aqueous or lipophilic. The method of the present invention also permits one to encapsulate a wide variety of materials, such as pigments, dye molecules, and aluminum flakes used in metallized paints.

Abstract: The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, and seed thereof, transformed with the nucleic acid molecules of the invention so as to confer disease resistance on the plant.

Abstract: A process for preparing beta-fructofuranosidase enzyme and a process for producing fructooligosaccharides, in which the preparation of the enzyme is obtained by cultivating the fungus Aspergillus niger, either wild or mutated, in a preferably semi-solid culture medium, in order to produce an extracellular enzyme, which is submitted to transfructosylation for producing fructooligosaccharides comprising sugars which are formed by one unit of sacrose and by two, three and four units of fructose.

Abstract: Genes have been isolated from strain DC260, a member of the Enterobacteriaceae family, encoding geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), phytoene synthase (CrtB), phytoene desaturase (CrtI), lycopene cyclase (CrtY), ?-carotene hydroxylase (CrtZ), and zeaxanthin glucosyl transferase (CrtX) activity. The genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.

Abstract: The present invention is generally directed to luminescent lanthanide compounds with phosphine oxide, phosphine oxide-sulfide, pyridine N-oxide, and phosphine oxide-pyridine N-oxide ligands. It also relates to electronic devices in which the active layer includes a lanthanide complex.

Abstract: Primary and auxiliary lens assemblies are provided that allow a wearer easy usage with a reduced risk of misalignment or detachment through the use of micromagnets. Conventional magnetic auxiliary lens assemblies are difficult to manufacture and functionally limited due to the limitations of conventional magnets and the composition of the auxiliary frames. The improved lens assemblies disclosed herein instead employ high strength micromagnets advantageously aligned and embedded in the auxiliary lens frame to improve the ease and reliability of coupling to a primary frame, and to provide aesthetic and functional advantageous over the known art.

Abstract: A method for displaying flipping pages of an electronic book via an electromechanical information browsing device that controls page flipping actions of information displayed as an e-book on a display device. The browsing device includes a controller that provides tactile feedback so as to lessen the user-interface differences between conventional books and e-books. Sensors and control mechanisms in the controller generate a series of electrical signals that are interpreted by the computer that displays the e-book to control various browsing operations, such as flipping pages, controlling a rate of page flipping, jumping to pre-set locations in the e-book, etc.

Abstract: A method of manufacturing an electro-active lens is disclosed. The lens is manufactured by providing a lens blank having a front and back surface, a thickness, and an index of refraction. An electro-active element is placed on one of the front or back surfaces of the lens blank. A covering surface is then formed over the surface of the lens blank containing the electro-active element. In some embodiments, the electro-active lens may then be surfaced to provide a desired fixed optical power and edged to fit within a spectacles frame.