Patents Assigned to Eppendorf Array Technologies
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Patent number: 8288128Abstract: The present invention relates to a method and apparatus for monitoring a real-time quantification of multiple target molecules during their binding on capture molecules of a micro-array. The method comprises the steps of: placing, in a chamber (14), a support (15) having fixed upon its surface a micro-array comprising at least 5 capture molecules (20) being immobilized in specifically localized areas (21) of said support; introducing said labeled target molecules solution (13) into the chamber; incubating said labeled target molecules under stable and controlled temperature conditions to allow the binding between said target and capture molecules; directing an excitation light (2) from a light source (1) on the surface of the micro-array; measuring the electromagnetic light emission (7) from the bound target molecules in response to said excitation light in presence of the solution containing the target molecules wherein the surface of emission for a localized area is comprised between about 0.Type: GrantFiled: November 18, 2004Date of Patent: October 16, 2012Assignee: Eppendorf Array Technologies S.A.Inventors: José Remacle, Isabelle Alexandre, Sylvain Margaine, Dieter Husar
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Patent number: 8247196Abstract: The present invention relates to a method and apparatus for monitoring on a micro-array a PCR amplification of a nucleotide molecule being present in a solution.Type: GrantFiled: November 18, 2005Date of Patent: August 21, 2012Assignee: Eppendorf Array Technologies S.A.Inventors: Jose Remacle, Isabelle Alexandre, Sylvain Margaine, Dieter Husar
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Patent number: 7892818Abstract: A method for obtaining an activation profile of a biological sample by disposing onto a solid support in a pre-determined spatial arrangement a subset of capture molecules able to interact with one or more activated transcription factor(s) present in the biological sample, contacting the biological sample upon the solid support under conditions allowing their interaction, monitoring signals resulting from their interaction, and providing a cellular activation profile from the detected signals.Type: GrantFiled: July 1, 2008Date of Patent: February 22, 2011Assignee: Eppendorf Array Technologies S.A.Inventors: Jose Remacle, Patricia Renard, Muriel Art
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Patent number: 7875442Abstract: Disclosed is a system and method conducting real-time PCR. Unlabeled capture molecules of a specific design are immobilized on a solid support, and contacted with amplicons produced in one or more PCR cycles. Detection of amplicons may take place during or between the PCR cycles while the solid support is in fluidic contact with the PCR solution. In an alternate embodiment detection of the amplicons takes place when the solid support is not in fluidic contact with the PCR solution. The method is suitable for the simultaneous detection and quantification of closely homologous target molecules.Type: GrantFiled: May 17, 2006Date of Patent: January 25, 2011Assignee: Eppendorf Array TechnologiesInventors: Isabelle Alexandre, Sylvain Margaine, Dieter Husar, Nathalie Zammatteo, Heinz Koehn, José Remacle
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Patent number: 7829313Abstract: Disclosed is a system and method conducting real-time PCR. Capture molecules of a specific design are immobilized on a solid support, and contacted with amplicons produced in one or more PCR cycles. Detection of amplicons may take place during or between the PCR cycles while the solid support is in fluidic contact with the PCR solution. In an alternate embodiment detection of the amplicons takes place when the solid support is not in fluidic contact with the PCR solution. The method is suitable for the simultaneous detection and quantification of closely homologous target molecules.Type: GrantFiled: May 24, 2006Date of Patent: November 9, 2010Assignee: Eppendorf Array TechnologiesInventors: Isabelle Alexandre, Sylvain Margaine, Dieter Husar, Nathalie Zammatteo, Heinz Koehn, José Remacle
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Publication number: 20100273678Abstract: An amplification and micro-array detection method is performed in a same buffer and/or in a same reaction chamber. A kit for an amplification of multiple nucleotide targets, uses low primer concentration.Type: ApplicationFiled: December 24, 2008Publication date: October 28, 2010Applicant: Eppendorf Array Technologies S.A.Inventors: Isabelle Alexandre, Neil Piront, Josè Remacle
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Publication number: 20100196358Abstract: The present invention is related to a method and kit (or device) for the detection and/or the quantification of biomarkers related to tumorigenesis. Said method is advantageously used to propose or adapt an anti-tumoral therapeutic protocol to be administered to a subject. Furthermore, said method and kit (or device) are a technical platform for the identification of new compounds, which are preferably used at (a) specific step(s) of an anti-tumoral therapeutic protocol.Type: ApplicationFiled: February 16, 2006Publication date: August 5, 2010Applicant: Eppendorf Array Technologies S.A.Inventors: Veronique Mainfroid, Jose Remacle
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Publication number: 20100137144Abstract: The present invention is related to a method for detecting and/or quantifying hierarchical (regulated) molecular changes of a cell in response to any external stimulus.Type: ApplicationFiled: June 23, 2006Publication date: June 3, 2010Applicant: Eppendorf Array Technologies S.A.Inventors: Jose Remacle, Vincent Bertholet, Sylvain Margaine, Francoise Delongueville, Christophe Van Huffel, Veronique Mainfroid, Christelle Plennevaux
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Publication number: 20100113301Abstract: The present invention is related to a method for the identification and/or the quantification of a target compound obtained from a sample, preferably a biological sample, comprising the steps of putting into contact the target compound with a capture molecule in order in order to allow a specific binding between said target compound with a capture molecule, said capture molecule being fixed upon a surface of a solid support according to an array comprising a density of at least 20 discrete regions per cm2, each of said discrete regions being fixed with one species of capture molecules, performing a reaction leading to a precipitate formed at the location of said binding, determining the possible presence of precipitate(s) in discrete region(s), and correlating the presence of the precipitate(s) at the discrete region(s) with the identification and/or a quantification of said target compound.Type: ApplicationFiled: December 1, 2009Publication date: May 6, 2010Applicant: Eppendorf Array TechnologiesInventors: Jose Remacle, Joseph Demarteau, Nathalie Zammatteo, Isabelle Alexandre, Sandrine Hamels, Yves Houbion, Francoise De Longueville
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Publication number: 20090239234Abstract: A method for identifying a genetically modified plant by identification and/or quantification of different, multiple nucleotide sequence elements corresponding to at least a portion of an exogenous nucleotide sequence integrated into the genome of the genetically modified plant. The method involves amplifying a nucleic acid sequence element into target nucleic acid sequences, contacting the target nucleic acid sequences with single stranded capture nucleotide sequences, detecting binding of the target nucleotide sequences, constructing a genetic map, and identifying the genetically modified plant.Type: ApplicationFiled: April 23, 2009Publication date: September 24, 2009Applicant: Eppendorf Array Technologies S.A.Inventors: Jose Remacle, Sandrine Hamels
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Publication number: 20090082223Abstract: A method for obtaining an activation profile of a biological sample by disposing onto a solid support in a pre-determined spatial arrangement a subset of capture molecules able to interact with one or more activated transcription factor(s) present in the biological sample, contacting the biological sample upon the solid support under conditions allowing their interaction, monitoring signals resulting from their interaction, and providing a cellular activation profile from the detected signals.Type: ApplicationFiled: July 1, 2008Publication date: March 26, 2009Applicant: Eppendorf Array Technologies S.A.Inventors: Jose Remacle, Patricia Renard, Muriel Art
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Publication number: 20080214407Abstract: A method quantifies a target compound selected from the group consisting of a polynucleotide or a protein present in a sample solution. The method includes putting into contact a target compound with a capture probe and detecting signals resulting from the binding between the target compound and its corresponding capture probe and resulting from the printed detection molecule in the different discrete regions. The method obtains a detection curve of the detected signals of the detection molecule and converts the signal obtained from the target compound bound to a specific capture probe into a concentration value and quantifies the target compound by converting the concentration value into a target amount using a target concentration curve.Type: ApplicationFiled: October 11, 2007Publication date: September 4, 2008Applicant: Eppendorf Array Technologies S.A.Inventors: Jose Remacle, Veronique Mainfroid, Sylvain Margaine
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Patent number: 7407748Abstract: The present invention relates to a method for obtaining an activation profile of a biological sample comprising disposing onto a solid support in a pre-determined spatial arrangement a subset of capture molecules able to interact with one or more activated transcription factor(s) present in the biology cell sample, contacting the biological sample upon the solid support under conditions allowing their interaction, monitoring signals resulting from their interaction, and providing a cellular activation profile from the detected signals.Type: GrantFiled: January 7, 2003Date of Patent: August 5, 2008Assignee: Eppendorf Array Technologies S.A.Inventors: José Remacle, Patricia Renard, Muriel Art
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Patent number: 7396643Abstract: The present invention is related to a screening, detection and/or quantification method of one or more transcriptional factor(s) (1) possibly present in a biological sample, said method comprising the steps of: possibly extracting and isolating said transcriptional factor (1) from said biological sample, putting into contact the transcriptional factor (1) with a double-stranded DNA sequence (2) bound to an insoluble solid support (3), and detecting and/or quantifying said fixed transcriptional factor (1), said double-stranded DNA sequence having a specific sequence able to be fixed by the transcriptional factor (1) and being preferably located at a distance of at least about 6.8 nm from the surface of the solid support (3), and said double-stranded DNA sequence being bound to the surface of the insoluble solid support (3) at a concentration of at least 0.01 pmole/cm2 of solid support surface (3). The present invention is also related to the kit comprising means and media for performing said method.Type: GrantFiled: March 23, 2001Date of Patent: July 8, 2008Assignee: Eppendorf Array Technologies, S.A.Inventors: José Remacle, Patricia Renard, Muriel Art
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Publication number: 20080085515Abstract: The present invention is related to a method for identifying and/or quantifying an organism or part of an organism in a sample by detecting a nucleotide sequence specific for said organism, among at least 4 other nucleotide sequences from other organisms or from parts of the organisms.Type: ApplicationFiled: March 30, 2007Publication date: April 10, 2008Applicant: Eppendorf Array Technologies SA (EAT)Inventors: Jose Remacle, Sandrine Hamels, Nathalie Zammatteo, Isabelle Alexandre, Francoise de Longueville
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Patent number: 7338763Abstract: The invention relates to a method and a kit for the specific identification and/or quantification of one or several among at least 7 organisms or parts thereof, in a biological sample being possibly contaminated by at least 4 other organisms, by detecting at least one nucleotide sequence specific of each of the organisms possibly present in said biological sample, wherein said nucleotide sequence is homologous with at least 4 other nucleotide sequences.Type: GrantFiled: June 2, 2004Date of Patent: March 4, 2008Assignee: Eppendorf Array Technologies S.A.Inventors: Jose Remacle, Sandrine Hamels, Sophie Burteau, Nathalie Zammatteo
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Patent number: 7321829Abstract: The present invention is related to a method for the identification and/or the quantification of a target compound obtained from a sample, preferably a biological sample, comprising the steps of putting into contact the target compound with a capture molecule in order to allow a specific binding between the target compound with a capture molecule, the capture molecule being fixed upon a surface of a solid support according to an array comprising a density of at least 20 discrete regions per cm2, each of the discrete regions being fixed with one species of capture molecules, performing a reaction leading to a precipitate formed at the location of the binding, determining the possible presence of precipitate(s) in discrete region(s), and correlating the presence of the precipitate(s) at the discrete region(s) with the identification and/or a quantification of the target compound.Type: GrantFiled: May 19, 2000Date of Patent: January 22, 2008Assignee: Eppendorf Array Technologies (E.A.T.)Inventors: José Remacle, Joseph Demarteau, Nathalie Zammatteo, Isabelle Alexandre, Sandrine Hamels, Yves Houbion, Francoise De Longueville
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Publication number: 20070298423Abstract: The present invention is related to a method for identifying and/or quantifying an organism or part of an organism in a sample by detecting a nucleotide sequence specific of said organism, among at least 4 other nucleotide sequences from other organisms or from parts of the organism.Type: ApplicationFiled: March 30, 2007Publication date: December 27, 2007Applicant: Eppendorf Array Technologies SA (EAT)Inventors: Jose Remacle, Sandrine Hamels, Nathalie Zammatteo, Isabelle Alexandre, Francoise de Longueville
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Patent number: 7205104Abstract: A method for identifying or quantifying an organism by a detecting its nucleotide sequence among at least 4 other homologous sequences comprising amplifying nucleic acids from the organism to generate target nucleotide sequences to be detected; contacting the target nucleotide sequences with single stranded capture nucleotide sequences bound by a single predetermined link to an insoluble solid support and discriminating the binding of a target nucleotide sequence specific of an organism with a signal resulting from a hybridization by complementary base pairing between the target nucleotide sequence and its corresponding capture nucleotide sequence is disclosed. The capture nucleotide sequence is bound to the insoluble solid support at a specific location on an array having a density of at least 4 different bound single stranded capture nucleotide sequences/cm2. The location of the signal on the array allows identification or quantification of the organism.Type: GrantFiled: March 23, 2001Date of Patent: April 17, 2007Assignee: Eppendorf Array Technologies SA (EAT)Inventors: José Remacle, Sandrine Hamels, Nathalie Zammatteo, Laurence Lockman, Sophie Dufour, Isabelle Alexandre, Francoise De Longueville
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Patent number: 7202026Abstract: The present invention is related to an identification and/or quantification method of a large number of biological organisms groups at different levels (family, genus, species) or part of those (possibly present in a biological sample) by a detection of their nucleotide sequence.Type: GrantFiled: January 23, 2002Date of Patent: April 10, 2007Assignee: Eppendorf Array Technologies SA (EAT)Inventors: José Remacle, Sandrine Hamels, Nathalie Zammatteo, Laurence Lockman, Sophie Dufour, Isabelle Alexandre, Francoise De Longueville