Abstract: The present invention relates to a method and apparatus for monitoring on a micro-array a PCR amplification of a nucleotide molecule being present in a solution.
Type:
Application
Filed:
November 18, 2005
Publication date:
September 13, 2012
Applicant:
EPPENDORF ARRAY TECHNOLOGIES
Inventors:
Jose Remacle, Isabelle Alexandre, Sylvain Margaine, Dieter Husar
Abstract: The present invention relates to a method, apparatus, cartridge and kit for monitoring on a micro-array a real-time PCR amplification of a polynucleotide molecule being present in a solution.
Type:
Application
Filed:
March 31, 2008
Publication date:
March 1, 2012
Applicant:
EPPENDORF ARRAY TECHNOLOGIES
Inventors:
Jose Remacle, Isabelle Alexandre, Sven De Roeck, Dieter Husar, Nathalie Zammatteo, Heinz Koehn
Abstract: Disclosed is a system and method conducting real-time PCR. Unlabeled capture molecules of a specific design are immobilized on a solid support, and contacted with amplicons produced in one or more PCR cycles. Detection of amplicons may take place during or between the PCR cycles while the solid support is in fluidic contact with the PCR solution. In an alternate embodiment detection of the amplicons takes place when the solid support is not in fluidic contact with the PCR solution. The method is suitable for the simultaneous detection and quantification of closely homologous target molecules.
Abstract: Disclosed is a system and method conducting real-time PCR. Capture molecules of a specific design are immobilized on a solid support, and contacted with amplicons produced in one or more PCR cycles. Detection of amplicons may take place during or between the PCR cycles while the solid support is in fluidic contact with the PCR solution. In an alternate embodiment detection of the amplicons takes place when the solid support is not in fluidic contact with the PCR solution. The method is suitable for the simultaneous detection and quantification of closely homologous target molecules.
Abstract: The present invention is related to a method for the identification and/or the quantification of a target compound obtained from a sample, preferably a biological sample, comprising the steps of putting into contact the target compound with a capture molecule in order in order to allow a specific binding between said target compound with a capture molecule, said capture molecule being fixed upon a surface of a solid support according to an array comprising a density of at least 20 discrete regions per cm2, each of said discrete regions being fixed with one species of capture molecules, performing a reaction leading to a precipitate formed at the location of said binding, determining the possible presence of precipitate(s) in discrete region(s), and correlating the presence of the precipitate(s) at the discrete region(s) with the identification and/or a quantification of said target compound.
Type:
Application
Filed:
December 1, 2009
Publication date:
May 6, 2010
Applicant:
Eppendorf Array Technologies
Inventors:
Jose Remacle, Joseph Demarteau, Nathalie Zammatteo, Isabelle Alexandre, Sandrine Hamels, Yves Houbion, Francoise De Longueville
Abstract: The present invention relates to a method and apparatus for monitoring on a micro-array a PCR amplification of a nucleotide molecule being present in a solution.
Type:
Application
Filed:
November 18, 2005
Publication date:
June 18, 2009
Applicant:
EPPENDORF ARRAY TECHNOLOGIES
Inventors:
Jose Remacle, Isabelle Alexandre, Sylvain Margaine, Dieter Husar