Abstract: The invention relates to a method and a kit for the specific identification and/or quantification of one or several among at least 7 organisms or parts thereof, in a biological sample being possibly contaminated by at least 4 other organisms, by detecting at least one nucleotide sequence specific of each of the organisms possibly present in said biological sample, wherein said nucleotide sequence is homologous with at least 4 other nucleotide sequences.
Type:
Grant
Filed:
June 2, 2004
Date of Patent:
March 4, 2008
Assignee:
Eppendorf Array Technologies S.A.
Inventors:
Jose Remacle, Sandrine Hamels, Sophie Burteau, Nathalie Zammatteo
Abstract: The present invention is related to a method for the identification and/or the quantification of a target compound obtained from a sample, preferably a biological sample, comprising the steps of putting into contact the target compound with a capture molecule in order to allow a specific binding between the target compound with a capture molecule, the capture molecule being fixed upon a surface of a solid support according to an array comprising a density of at least 20 discrete regions per cm2, each of the discrete regions being fixed with one species of capture molecules, performing a reaction leading to a precipitate formed at the location of the binding, determining the possible presence of precipitate(s) in discrete region(s), and correlating the presence of the precipitate(s) at the discrete region(s) with the identification and/or a quantification of the target compound.
Type:
Grant
Filed:
May 19, 2000
Date of Patent:
January 22, 2008
Assignee:
Eppendorf Array Technologies (E.A.T.)
Inventors:
José Remacle, Joseph Demarteau, Nathalie Zammatteo, Isabelle Alexandre, Sandrine Hamels, Yves Houbion, Francoise De Longueville
Abstract: The present invention is related to a method for identifying and/or quantifying an organism or part of an organism in a sample by detecting a nucleotide sequence specific of said organism, among at least 4 other nucleotide sequences from other organisms or from parts of the organism.
Type:
Application
Filed:
March 30, 2007
Publication date:
December 27, 2007
Applicant:
Eppendorf Array Technologies SA (EAT)
Inventors:
Jose Remacle, Sandrine Hamels, Nathalie Zammatteo, Isabelle Alexandre, Francoise de Longueville
Abstract: The present invention concerns a new and simplified method for obtaining a polymer surface activated with a high density of aldehyde functions which are stable with time and ready to react covalently with amino groups present on a great number of different molecules used as capture probes according to a microarray.
Type:
Application
Filed:
April 19, 2007
Publication date:
October 25, 2007
Applicant:
EPPENDORF ARRAY TECHNOLOGIES S.A.
Inventors:
Jose Remacle, Sandrine Hamels, Heinz Koehn
Abstract: A method for identifying or quantifying an organism by a detecting its nucleotide sequence among at least 4 other homologous sequences comprising amplifying nucleic acids from the organism to generate target nucleotide sequences to be detected; contacting the target nucleotide sequences with single stranded capture nucleotide sequences bound by a single predetermined link to an insoluble solid support and discriminating the binding of a target nucleotide sequence specific of an organism with a signal resulting from a hybridization by complementary base pairing between the target nucleotide sequence and its corresponding capture nucleotide sequence is disclosed. The capture nucleotide sequence is bound to the insoluble solid support at a specific location on an array having a density of at least 4 different bound single stranded capture nucleotide sequences/cm2. The location of the signal on the array allows identification or quantification of the organism.
Type:
Grant
Filed:
March 23, 2001
Date of Patent:
April 17, 2007
Assignee:
Eppendorf Array Technologies SA (EAT)
Inventors:
José Remacle, Sandrine Hamels, Nathalie Zammatteo, Laurence Lockman, Sophie Dufour, Isabelle Alexandre, Francoise De Longueville
Abstract: The present invention is related to an identification and/or quantification method of a large number of biological organisms groups at different levels (family, genus, species) or part of those (possibly present in a biological sample) by a detection of their nucleotide sequence.
Type:
Grant
Filed:
January 23, 2002
Date of Patent:
April 10, 2007
Assignee:
Eppendorf Array Technologies SA (EAT)
Inventors:
José Remacle, Sandrine Hamels, Nathalie Zammatteo, Laurence Lockman, Sophie Dufour, Isabelle Alexandre, Francoise De Longueville
Abstract: The present invention is related to a method for making microarrays comprising the steps of: submitting the surface of a solid support to an oxidation of chemical groups present on said surface in order to allow the formation of aldehyde functions upon the surface of said solid support, covalently binding upon said aldehyde functions capture molecules designed for the detection, the identification, the quantification and/or the recovery of complementary target biological or chemical molecules of interest; said covalent binding resulting in an array comprising a density of at least 4 or more discrete regions/cm2 of solid support surface, each of said discrete surface regions being bound with a species of capture molecules.
Type:
Grant
Filed:
April 10, 2001
Date of Patent:
May 23, 2006
Assignee:
Eppendorf Array Technologies SA (EAT)
Inventors:
Laszlo Hevesi, Laurent Jeanmart, José Remacle