Patents Assigned to Erasmus Universiteit Rotterdam
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Patent number: 9487830Abstract: The invention relates to the field of immunology and immunodiagnostics. Provided is a method for determining the replicative history of a lymphocyte, preferably a B cell, the method comprising detecting a signal joint nucleotide sequence on an extrachromosomal circular excision product in the lymphocyte, wherein the excision product is deleted from a chromosome to give a chromosomal-coding joint nucleotide sequence, wherein the coding joint is retained in the chromosome, and detecting the coding joint nucleotide sequence in the lymphocyte. Also provided are primers, probes and a control cell for use in a method of the invention.Type: GrantFiled: April 24, 2006Date of Patent: November 8, 2016Assignee: Erasmus Universiteit RotterdamInventors: Jacobus J. M. van Dongen, Thomasz Szczepanski
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Publication number: 20140037616Abstract: The present invention relates to a method for the generation of single chain immunoglobulins in a mammal. In particular, the present invention relates to a method for the generation of single chain camelid VHH antibodies in a mammal which undergo the process of class-switching and affinity maturation found within antibody producing B cells. Single chain antibodies generated using the method of the present invention and the uses thereof are also described.Type: ApplicationFiled: July 26, 2013Publication date: February 6, 2014Applicant: Erasmus Universiteit RotterdamInventor: Frank Grosveld
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Publication number: 20140033335Abstract: The present invention relates to a method for the generation of single chain immunoglobulins in a mammal. In particular, the present invention relates to a method for the generation of single chain camelid VHH antibodies in a mammal which undergo the process of class-switching and affinity maturation found within antibody producing B cells. Single chain antibodies generated using the method of the present invention and the uses thereof are also described.Type: ApplicationFiled: July 30, 2013Publication date: January 30, 2014Applicant: Erasmus Universiteit RotterdamInventor: Frank Grosveld
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Patent number: 8507748Abstract: The present invention relates to a method for the generation of single chain immunoglobulins in a mammal. In particular, the present invention relates to a method for the generation of single chain camelid VHH antibodies in a mammal which undergo the process of class-switching and affinity maturation found within antibody producing B cells. Single chain antibodies generated using the method of the present invention and the uses thereof are also described.Type: GrantFiled: July 6, 2010Date of Patent: August 13, 2013Assignee: Erasmus Universiteit RotterdamInventor: Frank Grosveld
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Patent number: 8502014Abstract: The present invention relates to a method for the generation of single chain immunoglobulins in a mammal. In particular, the present invention relates to a method for the generation of single chain camelid VHH antibodies in a mammal which undergo the process of class-switching and affinity maturation found within antibody producing B cells. Single chain antibodies generated using the method of the present invention and the uses thereof are also described.Type: GrantFiled: July 9, 2010Date of Patent: August 6, 2013Assignee: Erasmus Universiteit RotterdamInventor: Frank Grosveld
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Patent number: 7993850Abstract: The invention relates to the detection of fusion proteins. Described are a set of at least a first and a second molecular probe, each probe provided with a dye wherein the dyes together allow energy transfer, at least one probe provided with a reactive group allowing juxtaposing at least the first and second probes wherein the reactive group allows modulation of juxtaposing the probes such that there is an increased likelihood of energy transfer between the dyes. A method is provided which permits detecting the presence of a fusion protein in a cell at the single cell level.Type: GrantFiled: August 6, 2009Date of Patent: August 9, 2011Assignee: Erasmus Universiteit RotterdamInventor: Jacobus Johannes Maria van Dongen
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Patent number: 7820374Abstract: A method is provided for determining whether an agent is capable of inducing a DNA lesion in a eukaryotic cell, including exposing the eukaryotic cell to the agent and determining whether an HR23 protein-binding molecule accumulates in the cell, where the HR23 protein-binding molecule is preferably xeroderma pigmentosum group C (XPC), 3-methyladenine DNA glycosylase (MAG), CREB, p53, or a functional part, derivative, and/or analogue thereof. Preferably the cell overexpresses HR23A and/or HR23B protein. A rapid and sensitive test is provided with significant advantages over the Ames test. A method is provided for determining whether an agent is capable of inhibiting a cellular process, the process resulting in accumulation of HR23 protein-binding molecule within a cell. A method for determining whether a cell has an impaired DNA repair system is provided. An impaired DNA repair system is indicative for diseases such as xeroderma pigmentosum, cockayne syndrome, and/or trichothiodystrophy.Type: GrantFiled: November 20, 2002Date of Patent: October 26, 2010Assignee: Erasmus Universiteit RotterdamInventors: Jan H. J. Hoeijmakers, Steven Bergink, Gijsbertus Theodoras Johannes van der Horst, Wim Vermeulen, Mei Yin Ng
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Patent number: 7651480Abstract: Assembly of a needle (1) and a fluid supply device (7b), the needle (1) being provided with needle coupling means (2), the supply device (7b) being provided with a nozzle (9b), the needle coupling means (2) comprising a standardized construction with standardized internal dimensions according to the Luer standard, the needle coupling means (2) further comprising a projection (5), which renders fitting the needle (1) to a supply device with a nozzle (7a) arranged for cooperation with needle coupling means having standardized construction and dimensions according to the Luer standard impossible, the nozzle (7b) of the supply device (9b) being lengthened relative to a standardized nozzle (9a), such that the needle coupling means (2) with projection (5) can still be coupled to the lengthened nozzle (9b). The invention further relates to a needle (1), a syringe (7b), a method for performing an intraneural puncture and a method for preparing an insertion device for intraneural administration.Type: GrantFiled: September 17, 2001Date of Patent: January 26, 2010Assignee: Erasmus Universiteit RotterdamInventors: Cathelijne Dine Augustijn, Christiaan Johannes Snijders, Wilhelmus Sigebertus C. J. M. Van der Pol
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Publication number: 20090305305Abstract: The invention relates to the detection of fusion proteins. Described are a set of at least a first and a second molecular probe, each probe provided with a dye wherein the dyes together allow energy transfer, at least one probe provided with a reactive group allowing juxtaposing at least the first and second probes wherein the reactive group allows modulation of juxtaposing the probes such that there is an increased likelihood of energy transfer between the dyes. A method is provided which permits detecting the presence of a fusion protein in a cell at the single cell level.Type: ApplicationFiled: August 6, 2009Publication date: December 10, 2009Applicant: Erasmus Universiteit RotterdamInventor: Jacobus Johannes Maria van Dongen
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Patent number: 7575874Abstract: This invention relates to the detection of fusion proteins. The invention provides a set of at least a first and a second molecular probe, each probe provided with a dye wherein the dyes together allow energy transfer, at least one probe provided with a reactive group allowing juxtaposing at least the first and second probes wherein the reactive group allows to modulate of juxtaposing the probes such that there is an increased likelihood of energy transfer between the dyes. A method is provided which permits detecting the presence of a fusion protein in a cell at the single cell level.Type: GrantFiled: May 5, 2005Date of Patent: August 18, 2009Assignee: Erasmus Universiteit RotterdamInventor: Jacobus Johannes Maria van Dongen
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Publication number: 20080305965Abstract: The present invention relates to method of genetic analysis for the classification, diagnosis and prognosis of acute myeloid leukemia (AML). The invention provides a method for producing a classification scheme for AML comprising the steps of a) providing a plurality of reference samples, said reference samples comprising cell samples from a plurality of reference subjects affected by AML; b) providing reference profiles by establishing a gene expression profile for each of said reference samples individually; c) clustering said individual reference profiles according to similarity; and d) assigning an AML class to each cluster. The invention further relates to a method for classifying the AML of an AML affected subject, to a method for diagnosing AML in a subject, and to a method of determining the prognosis for an AML affected subject.Type: ApplicationFiled: February 23, 2005Publication date: December 11, 2008Applicant: ERASMUS UNIVERSITEIT ROTTERDAMInventors: Michael John Moorhouse, Petrus Jaobus Maria Valk, Hendrik Rudolf Delwell, Bob Lowenberg, Petrus Johannes van der Spek
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Publication number: 20070087333Abstract: The invention relates to a novel non-radioactive method to detect cytolytic activity that provides a measure of the existence and magnitude of an immune response against a particular antigen or immunogen. Provided is a method for detecting cytolytic activity of cells or a substance against a population of target cells, comprising the steps of providing target cells with a first nucleic acid sequence encoding a reporter molecule and a second nucleic acid sequence encoding an antigen of interest; co-culturing the target cells with a sample containing cells or a substance suspected of having cytolytic activity; and detecting the viability of target cells provided with the reporter molecule. Also provided are a kit and a nucleic acid for use in a method according to the invention.Type: ApplicationFiled: August 18, 2006Publication date: April 19, 2007Applicant: Erasmus Universiteit RotterdamInventors: Robertus Gruters, Carel van Baalen, Gustaaf Rimmelzwaan, Albertus Dominicus Marcellinus Osterhaus
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Publication number: 20070003951Abstract: The invention relates to the field of immunology and immunodiagnostics. Provided is a method for determining the replicative history of a lymphocyte, preferably a B cell, the method comprising detecting a signal joint nucleotide sequence on an extrachromosomal circular excision product in the lymphocyte, wherein the excision product is deleted from a chromosome to give a chromosomal-coding joint nucleotide sequence, wherein the coding joint is retained in the chromosome, and detecting the coding joint nucleotide sequence in the lymphocyte. Also provided are primers, probes and a control cell for use in a method of the invention.Type: ApplicationFiled: April 24, 2006Publication date: January 4, 2007Applicant: Erasmus Universiteit RotterdamInventors: Jacobus van Dongen, Thomasz Szczepanski
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Patent number: 7034144Abstract: The invention relates to the field of cytogenetics and the application of genetic diagnostic techniques in pathology and hematology. Specifically, the invention relates to nucleic acid probes that can be used in hybridization techniques for the detection of chromosomal aberrations and other gene rearrangements such as immunoglobulin and T-cell receptor gene rearrangements. The probes provided by the invention are a distinct and balanced set of probes of comparable size, each preferably being from 1 to 100 kb, or smaller, and flanking a potential breakpoint in a chromosome.Type: GrantFiled: December 18, 2003Date of Patent: April 25, 2006Assignee: Erasmus Universiteit RotterdamInventors: Jacobus Johannus Maria van Dongen, Anthonie Willem Langerak
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Publication number: 20050260660Abstract: This invention relates to the field of detecting interacting molecules. The invention provides a set of a first and a second probe, each such probe provided with a dye allowing energy transfer; at least one probe provided with a reactive group allowing juxtaposing the first and second probe. A method is provided for detecting at least two interacting molecules at the single cell level using of a set of probes according to the invention.Type: ApplicationFiled: May 5, 2005Publication date: November 24, 2005Applicant: Erasmus Universiteit RotterdamInventors: Jacobus van Dongen, Frank Staal
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Publication number: 20050260684Abstract: This invention relates to the detection of fusion proteins. The invention provides a set of at least a first and a second molecular probe, each probe provided with a dye wherein the dyes together allow energy transfer, at least one probe provided with a reactive group allowing juxtaposing at least the first and second probes wherein the reactive group allows to modulate juxtaposing the probes such that there is an increased likelihood of energy transfer between the dyes. A method is provided which permits detecting the presence of a fusion protein in a cell at the single cell level.Type: ApplicationFiled: May 5, 2005Publication date: November 24, 2005Applicant: Erasmus Universiteit RotterdamInventor: Jacobus van Dongen
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Patent number: 6921751Abstract: The invention relates to the field of immunology. Specifically, the invention relates to the field of immune-mediated disorders such as allergies, auto-immune disease, transplantation-related disease or inflammatory disease. The invention provides for an immunoregulator (IR), use of an IR in preparing a pharmaceutical composition for treating an immune-mediated disorder and a method for treating an immune-mediated disorder.Type: GrantFiled: November 20, 2000Date of Patent: July 26, 2005Assignee: Erasmus Universiteit RotterdamInventors: Nisar Ahmed Khan, Hubertus Franciscus Josef Savelkoul, Robbert Benner
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Patent number: 6844315Abstract: The invention relates to the field of immunology. Specifically, the invention relates to the field of immune-mediated disorders such as allergies, auto-immune disease, transplantation-related disease or inflammatory disease. The invention provides for an immunoregulator (IR), use of an IR in preparing a pharmaceutical composition for treating an immune-mediated disorder and a method for treating an immune-mediated disorder.Type: GrantFiled: March 29, 2001Date of Patent: January 18, 2005Assignee: Erasmus Universiteit RotterdamInventors: Nisar Ahmed Khan, Robbert Benner
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Patent number: 6808881Abstract: The present invention relates to prognostic method and means for determining susceptibility to heart disease in a subject by screening for polymorphisms in the Vitamin D receptor gene. In particular, the present invention provides a method for determining susceptibility to heart disease, the method comprising analysing the genetic material of a subject to determine which of the B/b, A/a or T/t alleles of the restriction enzyme sites BsmI, ApaI and TaqI respectively are present. Specific combinations of alleles represent a haplotype which is associated with susceptibility to heart disease.Type: GrantFiled: May 30, 2001Date of Patent: October 26, 2004Assignee: Erasmus Universiteit RotterdamInventors: Andreas Gerardus Uitterlinden, Johannes Petrus Thomas Maria Van Leeuwen, Huibert Adriaan Pieter Pols
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Patent number: 6803197Abstract: The present invention relates to a prognostic method and means for determining susceptibility to bone damage in a subject, by screening for polymorphisms in the Vitamin D receptor or collagen I&agr;1 genes. In particular, the method for determining susceptibility to bone damage comprises analyzing the genetic material of a subject to determine which of the B/b, A/a or T/t alleles of the restriction enzyme sites BsmI, ApaI and TaqI respectively are present. The method may further comprise determining which allele is present at die SpI restriction site of the collagen I&agr;1 gene. Specific combinations of the above alleles represent a haplotype which is associated with susceptibility to bone damage.Type: GrantFiled: May 10, 2001Date of Patent: October 12, 2004Assignee: Erasmus Universiteit RotterdamInventors: Andreas Gerardus Uitterlinden, Johannes Petrus Thomas Maria Van Leeuwen, Huibert Adriaan Pieter Pols